Poster

P139

P141

Tissue engineering an insinuating

science fiction
R. Pandurang Dhonnar*, R. S. Dolas,
S. N. Santhosh Kumar, A. R. Agrawal,
S. A. Baig, S. Bagade
Dr. D.Y. Patil Dental College And Hospital, Dr. D.Y. Patil Prathisthan, Pimpri,
Pune 411018, India

The effect of calcium phosphate surface

treatment of bioactive glass on adipose
stem cell differentiation into osteogenic
lineages
S. P. Haimi*, S. Miettinen, E. Pirhonen,
L. Moimas, Y. R. Lou, R. Suuronen
Regea, University of Tampere Biokatu 12,
33520 Tampere, Finland

Tissue Engineering or Regenerative Medicine has emerged as a new and exciting

alternative for Maxillofacial Reconstruction. Its a new option that supplemenst
existing treatment for reconstruction of
teeth, periodontium, bones, soft tissues,
salivary glands, Tempromandibularjoint,
blood vessels, muscles, tendons and nerves.
It draws support from multiple disciplines,
including cell culture and extra-cellular
matrix biochemistry. This is done in conjunction with molecular cell biology and
genetic engineering that are further integrated into basic concepts of chemical,
mechanical and material sciences engineering. We should be aware of these advances
and become an active participant in translation of this technology into the area of
Maxillofacial Reconstruction.

In this study, the effects of calcium phosphate surface treatment of 3D bioactive

glass scaffolds on the distribution, proliferation and differentiation of adipose
stem cells was investigated. Adipose stem
cells were found to attach, spread and
proliferate on the all scaffold types as
observed by fluorescent staining. Examination using scanning electron microscopy provided evidence of cells on the
surfaces and in the middle of the scaffolds. Alkaline phosphate activity and
staining showed osteogenic differentiation in each scaffold type. Our results
indicate that bioactive glass scaffolds
with or without calcium phosphate surface treatment can be used in bone tissue
engineering.
doi:10.1016/j.ijom.2007.09.142

doi:10.1016/j.ijom.2007.09.140

P140

P142

Effect of recombinant Adv.CMV.HGF

with gelatin sponge on the repair in the
temporomandibular joint
S. H. Shin*, I. K. Chung, H. S. Seong,
H. J. Lee, D. S. Hwang, B. J. Kim
Department of Oral & Maxillofacial Surgery, School of Dentistry, Pusan National
University, Pusan, Republic of Korea

Alveolar bone regeneration using

absorbable poly(L-lactide-co-ecaprolactone)/Alphagamma TCP
membrane and bFGF incorporatinggelatin sponge
An experimental study in the canine
mandible
Y. Kinoshita*, M. Matsuo, S. Ozono,
K. Tomihata, T. Shimamoto, Y. Ikada
Institute for Frontier Oral Science, Kanagawa Dental College, Japan

Objective: To investigate the therapeutic

use of Hepatocyte growth factor
(Adv.CMV.HGF) in temporomandibular
joint disc defect. Methods: Twelve white
rabbits were used. Defects (2 mm in diameter) were created in their TMJ discs.
Recombinant Adv.CMV.HGF with gelatin sponge (Gelfoam) as carrier was
implanted in the defects. Results: The
experimental group showed a significant
increase in the number of chondroblasts
and active cell differentiation at the margin of the defects. And chondroblasts
increased and chondrocytes showed a
columnar arrangement, which is witnessed
at the time of cell differentiation. Conclusion: Avd.CMV.HGF may be useful in the
repair of articular disc of the rabbit TMJ.
doi:10.1016/j.ijom.2007.09.141

We evaluated the effects of combining a

porous poly(L-lactide-co-e- caprolactone)/
Y
-TCP membrane with a bFGF incorA
porating -gelatin sponge on bone regeneration in mandibular ridges. Domeshaped membranes and the gelatin
sponges were implanted in full-thickness
saddle-type defects created in mandibular
alveolar ridges of beagles. Three and 6
months later, bone regeneration was
examined by soft X-ray, micro-CT, pQCT,
and histologically. New-bone volume and
density were higher in the experimental
group than in the control. Inflammatory
infiltration in the surrounding tissue of the
membranes was slight. Based on these
promising findings we suggest, that this

1109

technique will be applicable for alveolar

ridge reconstruction.
doi:10.1016/j.ijom.2007.09.143

TMJ
P143
Management of massive TMJ
reankylosis a case report
A. V. Akhilesh*, T. Joseph, R. Nair,
M. J. Michael
Department of OMFS, Govt. Dental College, Calicut 673008, India
Ankylosis of TMJ is a devastating affliction, which usually affects children, the
most common cause being childhood
trauma and infection. The afflicted joint
gets ankylosed resulting in functional
debility and facial deformity due to compromised growth. The typical clinical features are trismus and retruded lower jaw
amounting to almost a birds face appearance. We hereby present a case of Massive
Bilateral TMJ Re-ankylosis with medial
extensions of 3.5 cm on right side and
2.5 cm on the left side which was previously treated with resection and interpositional arthroplasty with silastic implants.
The clinical features, roentgenographic
features and management are discussed.
doi:10.1016/j.ijom.2007.09.144

P144
Condylar hyperplasia a report of two
cases
A. Gandhi*, S. Girish Rao
Department of Oral and Maxillofacial
Surgery, R.V. Dental College and Hospital, Bangalore, India
Objective: To present two cases of condylar hyperplasia treated with high condylectomy without disturbing mandibular
function. Methods: Two patients a male
and a female with condylar hyperplasia
were treated with high condylectomy after
appropriate investigations. Results: Both
patients had excellent facial appearance,
acceptable occlusion and painless temporomandibular joint function. Conclusions:
In cases of proven abnormally high growth
activity high condylectomy gives an opportunity to restrict excessive growth and
examine the condylar area histologically.
This surgery in itself may be adequate or
may be followed by further surgery.
doi:10.1016/j.ijom.2007.09.145