Professional Documents
Culture Documents
, 1 (1):
47-62,
Winter 2015
Global
J. Environ.
Sci. Manage., 1 (1): 47-62, Winter 2015
ISSN 2383 - 3572
A. Tariq; 2*M. Athar; 1J. Ara; 3V. Sultana; 4S. Ehteshamul-Haque; 5M. Ahmad
revised 20 November 2014; accepted 30 November 2014; available online 1 December 2014
ABSTRACT: In the present study ethanol and water extracts of 15 seaweeds, Dictyota dichotoma var. velutricata,
Dictyota indica, Iyengaria stellata, Padina pavonia, Sargassum swartzii, Sargassum variegatum, Stoechospermum
marginatum, Stokeyia indica, Jolyna laminarioides, Caulerpa taxifolia, Halimeda tuna, Ulva fasciata, Ulva lactuca,
Solieria robusta, and Melanothamnus afaqhusainii, were evaluated for their antioxidant potential by ABTS, superoxide
and total antioxidant capacity (TAC) assays. The activity was concentration dependent and the variation in antioxidant
potential was also observed by different assays in both extracts. Ethanol extract of D. dichotoma var. velutricata, D. indica
and S. marginatum demonstrated highest activity by TAC assay. The antioxidant potential in organic solvent fractions of
seaweeds namely P. pavonia, S. swartzii, S. marginatum and M. afaqhusainii was also determined and chloroform fraction
of all the four seaweeds showed highest activity by superoxide assay. Antioxidant activity of extracted fractions of
polysaccharides from S. indica, C. taxifolia and D. dichotoma var. velutricata was also evaluated by superoxide method.
Polysaccharide fractions of S. indica obtained from HCl (at 70 0C and room temperature) and water extract demonstrated
highest activity respectively. All the polysaccharide fractions of C. taxifolia showed excellent activity except CaClF70 C.
Polysaccharide fractions of D. dichotoma var. velutricata also exhibited very good activity.
Keywords: Antioxidant activity, Karachi coast, Polysaccharide fractions, Seaweeds
INTRODUCTION
Membrane lipid peroxidation is induced by free
radicals or reactive oxygen species, which are formed
in the body as a result of many biochemical reactions
as well as in electron transport chain (Ewing et al.,
1989). These free radicals damage cells and begin a
series of chain reaction leading to oxidation of various
compounds like proteins of cell membrane, which
impair the function of various enzymes that are
responsible for maintaining membrane integrity and
neutralizing metabolic oxidative products.
Antioxidants play a vital role against various diseases
like cancer, cardiovascular diseases, inflammation,
ageing process, rheumatoid arthritis, diabetes, as well
as disease associated with cartilage and Alzheimers
*Corresponding Author Email: athar.tariq@cdfa.ca.gov
Tel.: +1 916 262 0855; Fax: +1 916 262 0855
48
Antioxidant
Global J. Environ.
activity Sci.
and Manage.,
polysaccharides
1 (1): in
47-62,
seaweeds
Winterof2015
Karachi
Concentration (mg/ml)
1
Standards:
BHA
BHT
-tochopherol
Brown:
Dictyota dichtoma var. velutricata
Dictyota indica
Iyengaria stellata
Padina pavonia
Sargassum swartzii
Sargassum variegatum
Stoechospermum marginatum
Stokeyia indica
Jolynala minarioides
Green:
Caulerpa taxifolia
Halemida tuna
Ulva fasciata
Ulva lactuca
Red:
Solieria robusta
Melanothamnus afaqhusainii
LSD 0.05
Seaweed= 1.411
93.85 a 0.14
92.97 a 0.36
51.92 b 1.31
94.21 a 0.09
94.19 a 0.08
76.28 b 1.82
2.08 lm 0.50
3.03 jkl 1.10
1.03 m 0.25
6.93 fg 0.5
13.38 c 1.28
5.76gh 0.35
9.49 d 1.52
3.84 ijk 0.16
2.50 klm 0.71
5.8i 0.7
32.37 d 1.87
6.61 i 1.85
13.48 g 1.27
20.86 f 0.07
7.36 hi 0.46
24.42 e 1.63
8.25 hi 2.35
5.68 i 2.18
8.70 de 0.53
4.76 hi 1.34
0.95 m 0.80
3.29 ijkl 1.37
20.10 f
9.88 h
3.05 j
5.94 i
6.34 i 0.59
46.03 c 2.57
2.50
1.03
0.16
0.56
Concentration= 0.47 2
Mean values in column bearing same superscript letter are not significantly different according to Duncans multiple
range test (one way ANOVA).
1
Mean values in column showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
2
Mean values in row showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
Values are means Standard deviation with n=4.
Tariq et al.
Global J. Environ. Sci.A.Manage.,
1 (1): 47-62, Winter 2015
Table 2: Antioxidant activity of water extract of seaweeds by ABTS cation radical scavenging assay.
Antioxidant Activity (%)
Concentration (mg/ml)
Sample
Standards:
Ascorbic acid
94.27 a 0.21
Gallic acid
94.41 a 0.29
92.3 0.42
93.30 a 0.08
50.29 b 1.67
76.87 c 1.77
Dictyota indica
23.93 d 1.45
73.16 d 0.15
Brown:
Iyengaria stellata
Padina pavonia
8.61 2.24
54.87 g 2.73
15.83 e 2.00
53.42 g 2.5
Sargassum swartzii
16.69 1.99
61.27 f 0.07
Sargassum variegatum
23.55 d 1.83
86.93 b 2.76
Stoechospermum marginatum
15.02 1.48
67.83 e 2.11
Stokeyia indica
14.08 e 2.43
46.20 h 1.92
Jolynala minarioides
fg
8.43 1.02
24.54 j 1.24
27.00 c 0.81
71.32 d 1.65
1.03 i 0.92
3.32 l 2.53
Green:
Caulerpa taxifolia
Halemida tuna
Ulva fasciata
7.67 2.70
34.22 i 2.51
Ulva lactuca
6.03 fg 2.10
17.99 k 1.41
3.21 hi 0.0
26.49 j 2.30
fg
Red:
Solieria robusta
Melanothamnus afaqhussainii
LSD 0.05
Seaweed= 2.021
5.64
gh
0.0
17.02 k 1.96
Concentration= 0.69 2
Mean values in column bearing same superscript letter are not significantly different according to Duncans multiple
range test (one way ANOVA).
1
Mean values in column showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
2
Mean values in row showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
Values are means Standard deviation with n=4.
Concentration (mg/ml)
0.5
BHA
6.82 0.62(conc:0.05mg/ml)
N. T
BHT
26.56 1.56(conc:0.05mg/ml)
N. T
-tochopherol
12.29 0.61(conc:0.05mg/ml)
N. T
72.78 d 2.04
90.99 b 1.06
Dictyota indica
79.87 b1.75
96.73 a 1.89
Iyengaria stellata
23.95 m 0.63
29.31 k 1.45
Padina pavonia
73.30 d 2.47
97.12 a 2.54
Sargassum swartzii
94.44 a 1.26
99.03 a 0.53
Sargassum variegatum
36.02 k 0.09
55.45 j 0.79
Stoechospermum marginatum
69.46 e 1.31
97.79 a 1.31
Stokeyia indica
65.71 fg 1.43
75.95 g 0.55
Jolynala minarioides
27.04 l 0.22
76.99 fg 1.79
Caulerpa taxifolia
66.28 f 2.26
81.9 e 0.55
Halemida tuna
62.89 g 1.90
72.25 h 1.63
Ulva fasciata
40.16 j 2.46
88.25 c 2.60
Ulva lactuca
57.86 h 0.74
78.95 f 1.05
Solieria robusta
51.78 i 1.78
68.52 i 2.31
Melanothamnus afaqhusainii
76.37 c 2.62
85.38 d 1.78
Standards:
Brown:
Green:
Red:
LSD 0.05
Seaweed= 1.781
Concentration= 0.59 2
Mean values in column bearing same superscript letter are not significantly different according to Duncans multiple
range test (one way ANOVA).
1
Mean values in column showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
2
Mean values in row showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
Values are means Standard deviation with n=4.
Antioxidant
activity and
seaweeds
of Karachi
Global J. Environ.
Sci.polysaccharides
Manage., 1 (1):in47-62,
Winter
2015
Table 4: Antioxidant activity of water extract of seaweeds by superoxide anion radical scavenging assay.
Antioxidant Activity (%)
Concentration (mg/ml)
0.5
Sample
Standards:
Ascorbic acid
93.65 2.81(conc:0.05mg/ml)
N. T
Gallic acid
96.17 1.27(conc:0.05mg/ml)
N. T
71.87 c 1.99
91.8 b 1.29
Dictyota indica
71.47 c 0.32
86.18 cd 0.40
Iyengaria stellata
50.33 f 0.58
60.58 f 2.88
Padina pavonia
56.91 e 0.87
69.64 e 1.87
Sargassum swartzii
90.79 b 1.51
92.54 b 1.78
Sargassum variegatum
72.77 c 1.25
91.39 b 1.56
Stoechospermum marginatum
47.86 f 1.61
72.83 e 2.65
Stokeyia indica
93.82 a 2.6
97.05 a 1.96
Jolynala minarioides
39.91 g 1.61
87.50 c 0.83
Caulerpa taxifolia
60.11 d 1.11
82.82 d 2.68
Halemida tuna
34.88 h 0.96
85.67 cd 0.58
Ulva fasciata
34.65 h 2.4
72.32 e 1.52
Ulva lactuca
13.1 k 1.99
24.20 h 2.90
24.2 j 1.6
37.67 g 2.52
29.03 i 1.40
72.13 e 1.63
Brown:
Green:
Red:
Solieria robusta
Melanothamnus afaqhussainii
LSD 0.05
Seaweed= 2.071
Concentration= 0.71 2
Mean values in column bearing same superscript letter are not significantly different according to Duncans multiple
range test (one way ANOVA).
1
Mean values in column showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
2
Mean values in row showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
Values are means Standard deviation with n=4.
Sample
Brown:
Dictyota dichtoma var. velutricata
481.29 d1.15
648.25 d0.89
Dictyota indica
484.66 c0.44
652.44 c1.04
Iyengaria stellata
13.45 p0.85
22.50 p2.61
Padina pavonia
112.69 m1.44
242.57 i 1.8
Sargassum swartzii
295.44 f0.88
299.88 g0.55
Sargassum variegatum
149.31 k0.8
227.49 k0.59
Stoechospermum marginatum
437.83 e1.89
580.51 e0.81
Stokeyia indica
61.22 o0.23
233.77 j 1.11
Jolynala minarioides
10.09 q 2.89
32.87 o0.95
Caulerpa taxifolia
143.41 l 0.95
186.86 m2.95
Halemida tuna
290.96 g0.77
303.06 f0.78
Ulva fasciata
76.98 n 0.85
154.36 n1.29
Ulva lactuca
175.88 i 0.95
187.90 m0.52
Solieria robusta
215.17 h0.63
256.64 h1.21
Melanothamnus afaqhusainii
165.88 j 0.76
202.11 l 1.50
Green:
Red:
LSD 0.05
Seaweed= 1.641
Concentration= 0.56 2
Mean values in column bearing same superscript letter are not significantly different according to Duncans multiple
range test (one way ANOVA).
1
Mean values in column showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
2
Mean values in row showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
Values are means Standard deviation with n=4.
Sample
Brown:
Dictyota dichtoma var. velutricata
129.45 b0.63
325.24 b2.87
Dictyota indica
113.48 c0.83
211.53 d1.02
Iyengaria stellata
26.26 i 1.03
66.47 l 1.11
Padina pavonia
48.83 f0.29
127.94 g0.43
Sargassum swartzii
39.02 g0.63
91.79 j 1.05
Sargassum variegatum
83.99 d 0.63
138.88 e0.47
Stoechospermum marginatum
64.41 e0.76
106.69 i 0.25
Stokeyia indica
65.28 e0.13
134.83 f0.87
Jolyna laminarioides
15.66 j 0.25
52.49 n 2.36
Caulerpa taxifolia
49.68 f2.15
123.04 h0.49
Halemida tuna
26.73 i 0.60
56.11 m0.4
Ulva fasciata
33.08 h 0.75
71.72 k2.65
Ulva lactuca
4.29 l 2.64
15.76 p0.38
Solieria robusta
13.35 k1.23
42.99 o1.58
Melanothamnus afaqhusainii
82.76 d 1.64
233.34 c0.75
Green:
Red:
LSD 0.05
Seaweed= 1.511
Concentration= 0.53 2
Mean values in column bearing same superscript letter are not significantly different according to Duncans multiple
range test(one way ANOVA).
1
Mean values in column showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
2
Mean values in row showing difference greater than LSD values are significantly different at p<0.05(two way ANOVA).
Values are means Standard deviation with n=4.
Seaweeds
Brown:
Padina pavonia
Sargassum swartzii
Stoechospermum marginatum
Red:
Melanothamnus afaqhusainii
LSD
Ethanol extract
Hexane fraction
Chloroform fraction
Methanol fraction
73.30 b 2.47
94.44 a 1.26
69.46 b 1.31
83.93 a 0.37
88.08 b 1.05
77.83 a 2.18
83.81 a 0.58
95.38 a 0.27
77.75 a 0.55
39.72 c 0.57
83.31 c 0.36
-
76.37 c 2.62
2.47
70.98 d 0.76
1.60
89.11 a 0.42
2.45
82.63 b 0.61
2.66
Mean values in column bearing same superscript letters are not significantly different (p<0.05) according to Duncans multiple range test. 1Mean values in
row showing difference greater than LSD values are significantly different at p<0.05. N.T. =Not tested. Values are means Standard deviation with n=4.
Seaweeds
Brown:
Padina pavonia
Sargassum swartzii
Stoechospermum marginatum
Red:
Melanothamnus afaqhusainii
LSD
Ethanol extract
Hexane fraction
Chloroform fraction
Methanol fraction
31.85 c 1.16
49.42 a 1.37
57.08 a 2.85
32.59 c 0.21
37.07 c 0.37
39.83 c 0.27
44.09 a 0.18
22.28 d 0.62
46.34 b 0.17
34.86 b 0.18
44.47 b 0.39
-
45.78 a 1.27
1.14
43.19 b 0.26
1.50
36.31 c 0.47
2.70
42.82 b 0.22
1.32
Mean values in column bearing same superscript letters are not significantly different (p<0.05) according to Duncans multiple range test. 1Mean values in
row showing difference greater than LSD values are significantly different at p<0.05.
N.T. =Not tested.
Values are means Standard deviation with n=4.
Polysaccharides fractions
EFR
WF70C
HClFR
EF70C
WF R
CaCl2FR
HClF70C
H2 O Ext.
CaCl2F70C
LSD10.05
Mean values in column bearing same superscript letters are not significantly
different (p<0.05) according to Duncans multiple range test.
1
Mean values in column showing difference greater than LSD values are
significantly different at p<0.05.
Values are means Standard deviation with n=4.
WFR= Water fraction at room temperature; WF70C= Water fraction at 70C;
EFR= Ethanol fraction at room temperature; EF70C= Ethanol fraction at 70C;
HClFR= HCl fraction at room temperature; HClF70C= HCl fraction at 70C;
CaCl2FR= CaCl2 fraction at room temperature; CaCl2F70C= CaCl2 fraction at
70 C.
Mean values in column bearing same superscript letters are not significantly
different (p<0.05) according to Duncans multiple range test.
1
Mean values in column showing difference greater than LSD values are
significantly different at p<0.05.
Values are means Standard deviation with n=4.
WFR= Water fraction at room temperature; WF70C = Water fraction at 70C;
EFR= Ethanol fraction at room temperature; EF70C = Ethanol fraction at 70C;
HClFR= HCl fraction at room temperature; HClF70C= HCl fraction at 70C;
CaCl2FR= CaCl2 fraction at room temperature; CaCl2F70C = CaCl2 fraction at
70 C.
57
Antioxidant
activity and
seaweeds
of 2015
Karachi
Global J. Environ.
Sci. polysaccharides
Manage., 1 (1):in47-62,
Winter
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Antioxidant
activity and
seaweeds
of Karachi
Global J. Environ.
Sci.polysaccharides
Manage., 1 (1):in47-62,
Winter
2015
spectroscopy of carrageenans: application in research and
industry. Trends. Food. Sci., (13): 73-92 (20 pages).
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sulfated polysaccharides extracted from seaweeds at
various temperatures. Agric. Biol. Chem., (44): 589593 (5 pages).
Yan, X.; Nagata, T.; Fan, X.,(1998). Antioxidant activities
in some common seaweeds. Plant. Food. Human. Nutr.,
(52): 253-262 (10 pages).
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