Safety

Personal Protective Equipment

Glasses vs. Goggles

Gloves
Lab Coat
Face Shields

Safety Cabinets
Orientation

Gas Shuts off

Fire Extinguishers
Fire blankets
Eyewash/Shower

Biotechnology: A Laboratory Skills Course

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Laboratory Notebooks
Importance of
recording actual
data as it happens.
Formats
Purpose

Biotechnology: A Laboratory Skills Course

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Laboratory Notebooks
What to include
Numbered
procedures
Tables
Calculations with all
math and units
displayed
Sketches, model
numbers etc
Signatures
To and From

Biotechnology: A Laboratory Skills Course

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Equipment
Graduated Cylinders

Biotechnology: A Laboratory Skills Course

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Equipment
Volumetric Flasks

Biotechnology: A Laboratory Skills Course

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Equipment
Serological Pipet

Biotechnology: A Laboratory Skills Course

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Equipment
Pipet Pumps

Biotechnology: A Laboratory Skills Course

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Equipment
Transfer Pipets

Biotechnology: A Laboratory Skills Course

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Equipment
Burette

Biotechnology: A Laboratory Skills Course

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Equipment
Adjustable Micropipets

Biotechnology: A Laboratory Skills Course

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Equipment
Erlenmeyer flask

Biotechnology: A Laboratory Skills Course

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Equipment
Reagent Bottles

Biotechnology: A Laboratory Skills Course

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Equipment
Triple Beam Balance

Biotechnology: A Laboratory Skills Course

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Equipment
Electronic Balance

Biotechnology: A Laboratory Skills Course

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Equipment
Weigh Boats

Biotechnology: A Laboratory Skills Course

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Equipment
pH Meters

Biotechnology: A Laboratory Skills Course

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Equipment
Autoclave

Biotechnology: A Laboratory Skills Course

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How to Wash Glassware

Add small amount of soap and use a
brush to make sure all surfaces are
washed.
Rinse with tap water until no suds are
evident. Normally at least 3 times.
Rinse twice with distilled water.
Air dry or use oven to dry.

Biotechnology: A Laboratory Skills Course

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Labeling

Name of Solution
Concentration
Date made
Name of person who
made it
Potential Hazards if
necessary

Biotechnology: A Laboratory Skills Course

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Hazards
Universal
Hazards
Label

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Significant Figures
Rules

Biotechnology: A Laboratory Skills Course

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Significant Figures
Rules

Biotechnology: A Laboratory Skills Course

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Significant Figures
Rules

Biotechnology: A Laboratory Skills Course

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Scientific Notation
Shorthand method
to display numbers
that convey their
level of precision.
Uses a number
that is multiplied by
10 raised to a
positive or
negative number.

Biotechnology: A Laboratory Skills Course

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Units of Measure

Biotechnology: A Laboratory Skills Course

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Making Solutions
Percent Solutions
Mass to Volume

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Making Solutions
Percent Solutions
Volume to Volume

Biotechnology: A Laboratory Skills Course

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Making Solutions
Dilutions

Biotechnology: A Laboratory Skills Course

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Making Solutions
Molar Solutions
Proportional Method

Biotechnology: A Laboratory Skills Course

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Making Solutions
Molar Solutions
Unit Cancellation
Method

Biotechnology: A Laboratory Skills Course

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Diluting Molar Solutions

Biotechnology: A Laboratory Skills Course

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