Professional Documents
Culture Documents
LC Kingston Campus
LABORATORY
TY RULES
This is an important document. A faculty member and/or your coordinator will go through this
document as it pertains to your program annually. Each student must individually acknowledge
that they have downloaded, read and understood this document using the Blackboard link
provided by their program. Students will print and sign the Signature Form (at the back of this
document) indicating they have read and understood the rules. Students must ensure this
printed signed Signature Form is given to their coordinator. Every student must follow the
Laboratory Safety Rules as they pertain to everyone using the SLC Science Labs and the Labs at
the Kingston Human Society. Failure to obey the Laboratory Safety Rules can result in loss of
access to the facilities by the student at the discretion of the SLC employee witnessing the
infraction. This may also be reported to the Coordinator and Associate Dean of the School(s) as
necessary.
Approved By:f
Approved By: IlAte
Approved By `'
Date:
Date:
St. Lawrence
College
Table of Contents
General Lab Rules............................................................................ page 3
Biohazardous Areas Rules................................................................ page 4
Disposal of Needles and Other Blood Collection Devices page 5
Waste Disposal Methods. page 6
Biohazard Spill Clean Up................................................................ page 7
Zoonosis................................................................page 8-9
Access to Labs.................................................................................. page 10-11
Wound Procedures........................................... page 12
Puncture Wounds from Known Biohazardous Substances.. page 13
Incident / Accident Reporting Procedure......................................... page 13
Safety Systems and Equipment........................................................ page 14
X-ray Safety....................................................................... page 14
Emergency Equipment and Procedures.. page 15
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19.
Biohazardous Areas
Labs which contain the following biological materials are considered to be Biohazard Labs:
A.
Blood or any blood products (plasma, serum, controls) screened or unscreened.
B.
Other body fluids and biological materials (urine, feces, pusetc.)
C.
Bacteria, fungi, parasitic organisms
D.
Non preserved tissues
E.
Cell cultures
In addition to the GENERAL LAB RULES, the following rules apply to BIOHAZARD LABS:
1.
2.
3.
4.
5.
6.
Gloves must be worn when handling any biological materials containing blood or
blood products, clinical samples, unknown bacterial cultures or other soft tissue
materials. The gloves should be resistant to any chemicals being used in the
procedure. Gloves must be worn during all stages of phlebotomy training and during
subsequent collections.
-NON-LATEX GLOVES and/or NITRILE GLOVES are suitable for use with aqueous
solutions including blood, serum, and bacterial specimens. They will dissolve when
exposed to most organic solvents.
-VINYL gloves can be used for Histological staining procedures.
-POLYETHYLENE OR OTHER PLASTIC GLOVES should always be used if non-aqueous
reagents are used.
Note: Gloves must be worn at all times if your skin is NOT INTACT, e.g. cuts, broken
skin.
Consider used gloves as biohazardous waste. NEVER PUT USED GLOVES IN REGULAR
GARBAGE.
Keep all sample tubes upright and capped. Be sure not to contaminate the outer
surface. In the case of vacuum draw tubes, be sure no residual blood remains on the
top. Use spill disinfectant (red coded wash bottles) or alcohol wipes to clean any
suspect tubes.
Cap or seal all tubes being centrifuged.
To avoid creating aerosols do not pour biological samples. Use a pipette for transfers
and ensure that the outflow is against the side of the container and not directly on
the liquid surface.
Dispose of all biohazardous substances into appropriately labeled biohazard
containers. NEVER DISPOSE OF BIOHAZARDOUS SUBSTANCES INTO
REGULAR GARBAGE.
Place cap on flat surface while sharp is in use. Slide sharp end into the cap opening
Scoop the cap up and over sharp end so it completely covers needle
Secure cap using one of the following methods
a.
Secure cap at base of cap and needle using thumb and finger of hand holding
syringe
b.
Press tip of plastic cap against an inanimate object until it is firmly seated
on needle.
NOTE: Never use two hands to begin the recapping process. All needles are disposed into the
red or yellow safety approved needle disposal containers. PHLEBOTOMY SYRINGES MUST
NEVER BE PLACED IN REGULAR GARBAGE. All used syringes must be placed into the red or
yellow safety approved needle disposal containers.
METHOD OF DISPOSAL
Non contaminated
sharps/broken glassware
All biohazard waste MUST be discarded into the appropriate, leak proof waste container and
transported to the autoclave room (Rm 11835). Biohazard bins and lids must be wiped down
with spill disinfectant (red coded wash bottle) prior to transport. Students will be instructed on
the proper handling and transport techniques during their lab sessions.
NOTE: FOR CAMPUS BIOHAZARD WASTE DISPOSAL ENQUIRIES CONTACT GRACE WILDER
(MICROBIOLOGY) EXT. 1223 OR CELL PHONE #613-561-0642
Revised Summer 2014
ON BENCHES OR FLOORS:
B.
If spill is on clothing remove the affected clothing (if appropriate) and see the Professor
or Technical Staff.
C.
ON BOOKS/ NOTES:
Contaminated books, notes, personal items etc. must be soaked using SPILL
disinfectant (red coded bottles) or autoclaved: disposal may be required.
NOTE: When working in a biohazard lab area, please remember to clean your bench area with
bench disinfectant before and after each lab period. At the completion of each lab period
please ensure Bench Disinfectant is applied to adequately provide a wet layer for the
manufacturers suggested minimum contact time. Do not wipe dry.
Zoonosis
Zoonosis is defined as an infectious disease that is transmissible under normal conditions
from animals to humans and vice versa. Laboratory animals housed at St. Lawrence College
are specific pathogen free. The animals in the Cat/Dog colony at St. Lawrence College are
from unknown sources, but have received a health check and one set of vaccinations prior to
arriving.
Disease
Causative Agent
Hosts
Means of Spread
Brucellosis
B.canis
dogs
contact infected semen,
fetuses
Campylobacteriosis
C.fetus ,C.jejuni
dogs
ingestion
Leptospirosis
Leptospira spp.
rodents, dogs contact urine
contaminated soil and
water
Pasteurellosis
P.multocida
cats, dogs,
contact, bite wounds,
rabbits
inhalation
Pseudotuberculosis
Yersinia
rodents,
contact, contaminated
pseudotuberculosis
lagomorphs
food and water ingestion
Rat Bite Fever
S.moniliformis
rodents
Saliva, rodent bites,
ingestion
Salmonellosis
Salmonella spp.
Rodents,
ingestion, inhalation,
reptiles
contact
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Tetanus
Cl.tetani
dog, cat
Soil, bite wounds,
contaminated puncture
wounds
Tuberculosis
M.tuberculosis
dogs
contact, ingestion,
inhalation
Tularemia
F.tularensis
lagomorphs,
inhalation contact, tick
Rabbit fever
dogs
and insect bites,
ingestion of
contaminated food and
water
Rocky mountain
R.rickettsia
rabbits, dogs
tick bites: Dermacentor
spotted fever
spp
Rabies
Rabies virus
dogs, cats
bites; saliva contact
Coccidioidomycosis
Coccidioides immitis
dogs
inhalation of air-borne
spores; fungus present in
desert soil
Amebic dysentery
Entamoeba
dogs
contamination of food,
Disease
Causative Agent
histolytica
Hosts
Giardiasis
Giardia intestinalis
dogs
Histoplasmosis
Histoplasma
capsulatum
Toxoplasma gondii
dogs
Ringworm,
dermatomycosis
Trichophyton spp.
Microsporum spp.
Other dermatophytes
dog, cat,
rodents,
rabbits
Bartonella henselae
Toxacara canis/cati
Scabies Mite
Chlamydia psittaci
cats
dogs, cats
dogs
birds
Toxoplasmosis
cats
Means of Spread
usually by man (natural
host) to dogs
man is main reservoir,
ingestion of cysts in
contaminated water food
inhalation of fungi; may
also grow in soil
ingestion of oocysts from
cats; inhalation infected
meat; fetal transmission
may occur
direct contact, ringworm
of man can be
transmitted to animals
and visa-versa; soil may
be reservoir
bite/scratch
oral/fecal
direct Contact
inhalation of respiratory
secretions, droppings,
urine
B.
MAKE-UP LABS:
Make-up labs must be approved by the Professor and then a mutually agreeable time
MUST be arranged with the Technical Staff in the area. The student must consult with
the Faculty/Technical Staff at least 24 hours in advance to make arrangements to do
the make-up lab. (Note: Microbiology labs require a minimum of 48 hours for
culturing.) Students may not be alone in laboratory areas.
C.
VETERINARY LABS:
Veterinary tech/assistant students using the lab areas with permission can only be in a
lab outside of classroom time if they are in a minimum group of two people.
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NOTE: Direct supervision entails the presence of the identified faculty/support staff
person inside the lab where the students are working, or the presence of the
identified faculty/support staff person in the lab directly adjoining that. In the latter
case, the faculty/support staff person will ensure open access through the adjoining
door. Under no circumstances will a student be in a science laboratory alone.
IMPORTANT:
Phlebotomy is a Controlled Act. Collection of blood specimens must be directly supervised by
a CMLTO registered, practicing MLT who has phlebotomy listed as a specialty on their CMLTO
registration, or by another suitably trained MLA/T who has been specifically trained in, and
deemed competent in phlebotomy by a regulated health professional.
II. Attendance and Missed Labs (MLA/T and MLS):
It is MANDATORY that students attend and actively participate in scheduled laboratory periods
and clinical experience. In MLS and MLA/T courses containing a laboratory/clinical component,
missed laboratory/clinical time puts students at risk of not being able to demonstrate/be
assessed in the learning outcomes of the course. If absenteeism occurs, an acceptable
document of proof of reason for absence may be required. All competencies missed due to the
absence must be deemed demonstrated by the Instructor. Missed labs may result in a mark of
zero for the lab; therefore, persistent absenteeism will result in a non-continuance grade for
the course. Make up labs could occur in the evening and/or weekends. In order to cover the
cost of the new materials, support staff and Instructors time, there may be added cost incurred
by the student for making up practical lab activities. Any cost is determined at the end of the
semester by the Health Science Office.
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Wounds Procedures
In all severe cases: SEEK MEDICAL ATTENTION ASAP. CALL 5555 (SECURITY) AND THEY WILL
ASSESS THE SITUATION AND CALL 911 IF NECESSARY. When in doubt call 5555.
A.
WOUNDS involving MAJOR BLEEDING (e.g. arterial laceration)
Control bleeding by direct pressure.
Elevate affected area.
Put the person at rest. Use
compression/ ice CALL 5555
immediately
Fill out an accident/incident electronic form.
B.
WOUNDS involving MINOR BLEEDING (e.g. needle injury, scrape, and cut)
Get to a sink.
Allow the wound to bleed freely.
Scrub with soap and water and rinse well under running water.
Stop the bleeding and apply a bandage.
Report this to the professor and they will fill out an electronic accident/incident form.
C.
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Location and use of lab specific safety equipment will be demonstrated by professors. Students
should ensure they know the routes of EXIT for each of their labs in case of emergency.
X-Ray Safety
X-Ray safety rules follow provincial regulations of the Occupational Health and Safety Branch,
Ministry of Labour. A dosimeter will be assigned to each student. Students are required to wear
their dosimeter while taking X-rays (Either at the Kingston Humane Society or at St. Lawrence
College).
As per Ministry of Labour guidelines, no cassettes shall be hand held during an exposure. X-rays
will not be taken without the supervision of a trained staff/faculty member present. Protective
apron, gloves and a thyroid protector of at least 0.55 mm lead must be worn while taking an XRay. A record of radiographic exposures, including the date, kilovoltage, tube current and
duration of each exposure shall be maintained and kept for at least one year. Exposure reports
from the National Dosimetry Service (Health Canada) are maintained on file for a minimum of 7
years.
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2.
ELECTRICAL BURNS: DO NOT ENDANGER YOURSELF. Turn off the circuit breaker. The
breaker panel is located by the exit doors in most labs.
If possible, remove the person from the source of the current without endangering
other people.
3.
NOTE: Do not move anyone with unknown injuries except when there is danger of further
injury. GET HELP. Do not leave the injured person alone any longer that is necessary to get
help. An injured person may faint or go into shock even when the injury appears minor. If you
cannot leave- SHOUT.
During regular posted hours, contact any Professor or Technical Staff in the area or
contact Security at 5555.
All incidents should be reported using the E-Alert electronic reporting system.
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B.
C.
D.
All incidents should be reported using the E-Alert electronic reporting system.
CHEMICAL WASTE DISPOSAL:
Discard the waste in the appropriately labelled container as instructed by the Professor or
the Technical Staff.
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Evacuation Procedure for Veterinary Surgical Rooms (RM. 00693 AND 00650)
A.
IF THE ALARM SOUNDS IN THE MORNING AND THE SURGICAL PATIENT IS NOT
ANAESTHETIZED:
Dogs should be leashed, and cats should be put into a carrying case and
everyone should vacate the College as soon as possible via the nearest exit.
Go far away from the crowds and the building.
If you deem that the situation is an immediate emergency do not evacuate any animals.
All personnel should vacate the College immediately.
B.
The Ward people will leash dogs and carton cats and vacate the College as described
above.
The Surgeon/ professor, and technologist(s) will continue with the surgery.
Karoline Machado or Sarah Hatfield will advise the team as soon as possible whether
or not to evacuate. If evacuation is necessary, the surgical patient will be disconnected
from the anaesthetic machine and carried to safety by the surgical team.
Technical staff will advise the Professor as soon as possible whether or not to
evacuate. If evacuation is necessary, the anesthetized animal(s) will be humanely
euthanized as quickly as possible.
C.
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PREGNANCY:
Some labs may involve the use of hazardous materials. Many chemical and biological
materials which are only moderately toxic to adults may be extremely hazardous to a
foetus. Examples: volatile solvents, anaesthetics, some bacteria, radiation, heavy
metals, many organic materials. It is the responsibility of the student who knows or
suspects she is pregnant to inform her Professor/Technical Support persons as extra
precautions may be warranted in some lab functions.
B.
The school is not responsible for contacts washed down the sink during eye
wash Emergencies.
C.
ASTHMA / ALLERGIES:
It is recommended that students who have asthma or any type of allergies inform their
professor and the technical support staff. Due to the sharing of lab space, lab chemicals,
and lab materials/equipment and the possible exposure to animals this is important for
all students in all programs using the laboratories.
Students with asthma related issues due to exposure to animals and their bedding
should contact their coordinator for details on N95 mask fist testing.
D.
E.
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Telephone Directory
COLLEGE PHONE NUMBER:
SECURITY:
HEALTH CENTRE:
EXT. 1136/1182
KIM CLOUTIER
EXT. 1704
LYNDA ASHAUER
EXT. 1654
PETE JARDINE:
CARLENE LOTT
HELEN THOMPSON
KAROLINE MACHADO
GRACE WILDER
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Year: 2014
Signature:
Date: (09/09/2014)
Please self-identify in the space below if you have allergies or intend to wear contact lenses in the lab:
N/A
Students will be required to acknowledge that they have read and understand the Laboratory Safety
Rules by signing the Signature Form annually. A copy of the Signature Form(s) will be kept on file for the
duration of their program.
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