Thesis

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Gravitizer to The Rescue

The feasibility of Aqueous Extract of Guyabano (Anona Muricata L.) Peelings as a main
component in making a hand sanitizer
Esmaa, Jasmine J.
Roxas, Arianne Mae P.
Submitted in partial fulfillment of the requirements in Research II
Caloocan High School

10th Avenue Grace Park, Caloocan City
August 19 , 2013
Mrs. Viola B. Donato

Research Adviser

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Table of Contents
Preliminary Parts
Title Page
Table of Contents
Abstract
Dedication
Chapter I: Introduction
Background of the Study
Statement of the Problem
Significance of the Study
Scope and Delimitation of the Study

Chapter II
Review of Related Literature
Conceptual Framework
10
2

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Hypothesis
10
Definition of Terms
11
Chapter III: Methodology
12
Chapter IV: Results and Discussion

Statistical Analysis
14
Chapter V. Conclusion, Recommendation
27
Conclusion
16
Recommendation
16
Chapter VI
Bibliography
17
Acknowledgements
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Abstract
Microbes spread easily in our community by means of having physical contact
with a contaminated object, living or non-loving, which leads to the purpose of the
research study; to stop the increasing number of people who acquire diseases from
microbes. This study aims to produce a product that will prevent our society from
obtaining diseases that may result to unproductiveness. Researches had been
conducted regarding on the matter of killing and stopping microbes from spreading. The
main objective is to exterminate certain microbes naturally. The study promotes the use
of guyabano peelings as the main ingredient in the production of a natural hand
sanitizer. The utilized materials include the electric mixer, mixing bowl and utensils,
which are accessible at the researchers house. The ingredients used were purchased
from different private sellers. In producing the natural hand sanitizer, Gravitizer, the
guyabano peelings were obtained and washed. The guyabano peelings were blended to
obtain the peelings aqueous extract with less effort. The aqueous extract of the
peelings holds an anti-microbial property and vitamins which can improve our immunity.
The Aloe Vera Gel was mixed to the extract to avoid several skin complications. Mostly,
guyabano peelings are thrown away it will be much economical if we are able to create
something out of it. Almost everybody can produce and buy one. By the use of this
natural sanitizer we can improve our health and prevent microbes from harming our
well-being. As we all know, prevention is better than cure.

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Dedication
The researchers are dedicating this research to God, Our Father, for hes the one
who made this research possible and for giving us strength to continue this work.
The researchers are also dedicating this research to their Research Adviser, Mrs.
Viola B. Donato, who had been giving advices that helped the researchers accomplish
every challenge they met during the experiments.

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Introduction
Microbes are everywhere. They are naturally occurring in the soil, air, water,
human body, animals, insects and other non-living objects. They are known to spread
diseases to humans and animals alike. Microbes include parasites, bacteria, germs and
fungi.
The researchers conducted this study because they were concerned about the
widespread infections occurring to the society. The objective is to create a substance
that will help prevent future episodes of these incidents.
Background of the Study

In order for us to live, we must maintain a good health. The emergence of
diseases is a threat for being productive. A man does his activities remarkably when he
is healthy. And in order to do so, we must take prevention. The researchers aim to
produce an antimicrobial solution that will help eradicate certain bacteria that can help
microbes from harming our societys well-being.
The aqueous extract of the Guyabano fruit has an antibacterial property. It is
antimicrobial against Staphyllococcus aureus, a Gram positive cocci which is a natural

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flora of the human body especially the face, and Vivrio cholerae which is a positive
agent of cholera and causes diarrhea that can lead to dehydration, infection and
complication. Antimicrobial or bactericidal substance destroys bacteria.
Based from Wikipedia, hand sanitizer is an alternative for washing our hands with
soap and water. It can be prepared in form of gel, foam or liquid solution. The use of an
alcohol based hand sanitizer is more effective in killing microbes than soaps. It can
prevent the transmission of dangerous diseases.
Statement of the Problem
Is soursop or guyabano an essential ingredient to produce an effective hand sanitizer?
Significance of the Study
Obtaining certain illness and diseases from microbes can cause health
complications or even death. These cases can be also prevented by the use of a hand
sanitizer. The guyabano aqueous extract added to the hand sanitizer have antimicrobial
properties that can destroy certain bacteria that other commercialized hand sanitizers
cant.

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Review of Related Literature

This chapter is a review of the existing literature relevant to the topic of
Guyabanos potential. More specially, this chapter is a review of topics including: what
can guyabano contains, the effect of its extract.
In this part of research, are related ideas and topics about guyabano and how
can it affect our lives. The following information are gathered from different literatures,
both local and foreign.
Local Literature
Guyabano (Anona Muricata), or soursop in English, is a small tropical fruit tree
bearing a sweet, heart haped fruit with a soft-spine green outer skin and soft pulpy white
flesh. The fruit was tested against staphylococcus, e coli and other common bacterias
for anti-microbial capability and toxicity. (Malaya, 2012)
The fruit together with its leaves, stems, roots, seeds and bark is concocted into
expensive juices and capsules and is marketed to cure asthma, arthritis, heart, liver
diseases,. One literature said, that the guyabano extracts are 10,000 times stronger
than aadnamyan, a drug used in chemotherapy treatments. (Philippine Daily Inquirer,
2012).
Aloe Vera is very popular substance commonly used in shampoos, body lotions,
cosmetics and juice drinks. According to the book of Martindale: The extra
8

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pharmacopeia, considered the bible of pharmacology. It is widely used in cosmetics

and toiletries for it is reported moisturizing and revitalizing action. There are also claims
of the beneficial and even curative properties of aloe vera gel in the self-treatment of
medical conditions (Mirror Weekly, 1999).
Anona muricata have exhibited antibacterial activity against numerous
microorganisms such as Plasmodium falciparum, Escherichia coli, Pseudomonas
aeruginosa,
Shigella
flexneri,
Staphylococcus
aureus,
Staphylococcus
albus,
Salmonella typhosa, Salmonella newport, and Serratia macescens (Raintree Nutrition,

Inc. 2004).
The guyabano fruit is an excellent source of Vitamins B and C though the flesh of
this fruit was reported to be deficient in calcium, phosphorous and Vitamin A.
(Quisumbing, 1951).
Foreign Literature
Numerous studies have been conducted on the plant Anona muricata L. Due to its
wealth of cytotoxic cancer substances active against numerous cancer cell lines, this
Guyabano fruit had been given more attention than the others. (Reiser et al. 1993).
Anona muricata L. is known to have antimicrobial properties. The Guyabano
have antibacterial, antifungal, and antiviral potentials. (Taylor 2005).

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Conceptual Framework
Guyabano
Fruit
Extraction of
Guyabano
Aqueous
Extract
Gravitizer
(Guyabano
Hand
Sanitizer)
Hypothesis
* Operational
There is a significant difference between guyabano sanitizer and commercialized
sanitizer.
* Null
There is no significant difference between guyabano sanitizer and commercialized
sanitizer.
10

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Definition of terms
Guyabano - Guyabano fruit is a green, pear-shaped fruit covered with soft spines.
Matured guyabano or soursop fruit weighs about 2 to 5 kilograms. Guyabano fruit is
ovoid and large, up to 18 centimeters long, with thin skin and the soft edible whitish
pulp that is fleshy and fibrous. Guyabano has inedible black seeds. Guyabano fruit
or soursop fruit has distinct sweet-sour flavor that tastes like pineapple and
strawberry with a tang of sour citrus taste.

Aloe Vera - Aloe vera is a cactus plant that belongs to the Liliaceae family. It grows
in dry climates such as those found in parts of Africa and India and has been used
medicinally for centuries. Aloe leaves secrete a clear gel that when broken off from
the rest of the plant that can be applied topically to heal wounds and soothe skin.
Hand Sanitizer - is a supplement or alternative to hand washing with soap and
water. Various preparations are available, including gel, foam, and liquid solutions.
The active ingredient in hand sanitizers may be isopropanol, ethanol, n-propanol,
or povidone-iodine
Methodology
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Gathering of the Materials

Fresh guyabano fruits weighing 6kg were gained from a public market near the
researchers house. The 500 mL Aloe Vera gel was obtained from a private seller in
Muntinlupa City. Bowl, ladle, and hand sanitizer container were used in the experiment,
all of which are obtained from the researchers house.
Extraction of the Guyabano Aqueous Extract
After the separation of the peelings from the fruit, the researchers gained less
than half a kilo of peelings. The peelings were blended by the use of an electric mixer.
The peelings were placed on a cloth and was compressed manually. 25mL of aqueous
extract from the peelings was obtained
Mixing the Ingredients
Mixing of ingredients in the bowl one by one was done. The 25mL aqueous
extract gained was poured into the bowl and was followed by 75mL Aloe vera gel. It was
stirred and the solution was then transferred to a clean hand sanitizer container.
Experimental Design
12

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The researchers planned to have 10 random testers for the hand sanitizers. The
testers will apply both of the hand sanitizers but in different hand, for example, the
Gravitizer will be applied in the left part while the commercialized in the right part. After
testing both hand sanitizers, the researchers let the testers rate the hand sanitizers from
1-5. The data collected is shown below.
Tester
Commercialized
Gravitizer
10
10
Legend:
9-10 = Excellent
7-8 = Very Good
5-6 = Good
3-4 = Fair
1-2 = Poor
Results and Discussion

Statistical Analysis
Table
One of the most common statistical analyses, the T-test for Two Independent
5.1
Samples, is used to evaluate the mean difference between two populations using the
data from two seperate samples. The general purpose of the independent measures t
13

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test is to determine whether the sample mean difference obtained is a real difference
between the two populations or simply the result of sampling error.
Tester
Commercialize
Gravitizer
Table 5.2
d
)2
)2
The
-0.7
0.49
-1
calculated
-0.7
0.49
t is 2.7851.
0.7
0.49
With a
-0.7
0.49
0.7
0.49
0.7
0.49
-1
0.7
0.49
18 and p
0.7
0.49
of 0.5, t
0.7
0.49
must
0.7
0.49
63
degree of
freedom of
exceed or
82
be equal to
6.3
8.2
Standard
Deviation
1.6364
1.2993
Variance
2.6778
1.5111
2.101.
According
to the
results, tcalc>ttabular. The computated t-score of 2.7851 exceeds the tabular value of t, then
14

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the researchers conclude that there is a statistically significant probability that the
relationship between two variables exists and not due to chance, and reject the null
hypothesis.
The researchers then reject that there is no significant difference between the
commercialized sanitizer and Guyabano sanitizer and instead accept the research
hypothesis that there is a significant difference between the two santizers.
Conclusion
Based on the data gathered, the researchers therefore conclude that the
gravitizer is more effective than the commercialized hand sanitizer in terms of texture,
smell and moisturizing effect. The testers also commented the good effects that they felt
during the testing.
Recommendation
The researchers recommend to not only to use the gravitizer but to expand its
natural wonder. So that in the near future this study will be further more enhance and so
as the product which is the gravitizer.
15

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Bibiliography
- 21st Century Universal Encyclopedia; Vol.14 = Hav-Hyd; Sydney; JM Dent And Sons
Ltd Publishing 1983; 199
- Grolier International; Danbury, Connecticut; 1998
- New Standard Encyclopedia; Vol. 16 = Sma-Sz; Standard Education Corporation;
Chicago; 1996
- Encyclopedia Americana; Vol. 25
- Comptons Encyclopedia; Vol. 10; 1995; Chicago; Printed in USA; Comptons Learning
Company; Tribune Publishing Company
- Encyclopedia Brittanica; Cocker, Dais; Scotland; 1768
- Grolier International, Vol. 15
16

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- Guyabano: Pulpy, sweet and healthy;Malaya;November 23, 2012;p.A12

-Guyabano for cancer? ; Rafael Castillo; Philippine Daily Inquirer; May 5, 2012; p.B5
- Questions raised over used of Aloe Vera; Mirror Weekly; October 25, 1999; p.21
- Guyabano-Quezon City: DA[nd]- 12p.:111.photos;21cm; Department of Agriculture
- http://chemistry.about.com/od/cleanerchemistry/a/hand-sanitizer-recipe.html
- https://en.wikipedia.org/wiki/Glycerol
- http://www.wikihow.com/Make-Your-Own-Waterless-Hand-Sanitizer
- http://en.wikipedia.org/wiki/Aloe_vera
- http://www.mommypotamus.com/how-to-make-non-toxic-hand-sanitizer
- http://voices.yahoo.com/3-easy-hand-sanitizers-home-11173828.html
17

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Acknowledgement
The researchers would like to give their gratitude to their parents for supporting the
researchers financially, emotionally and intellectually. For encouraging the researchers
to accomplish this research.
Furthermore, the researchers would also like to thank Rozel Nikko Leao, MT, who
provided certain knowledge about the topic being studied by the researchers.
Most of all, the researchers would like to thank God, Our Father, for giving us the
strength and inspiration to continue this research until the end.
The researchers would also like to give their gratitude to Mrs. Viola B. Donato,
Research Adviser, who guided the researchers and gave sufficient knowledge for them
to accomplish this research that wouldn't have been possible without her assistance.
18

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Esmaa, Jasmine J.
Roxas, Arianne Mae P.
The Researchers
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Republic of the Philippines

Gravitizer to The Rescue

Submitted in partial fulfillment of the requirements in Research II

Caloocan High School

Mrs. Viola B. Donato

Republic of the Philippines

Statement of the Problem

Significance of the Study

Scope and Delimitation of the Study

Republic of the Philippines

Chapter III: Methodology

Chapter IV: Results and Discussion

Chapter V. Conclusion, Recommendation

Republic of the Philippines

Republic of the Philippines

Republic of the Philippines

Background of the Study

Republic of the Philippines

Republic of the Philippines

Review of Related Literature

Republic of the Philippines

pharmacopeia, considered the bible of pharmacology. It is widely used in cosmetics

Salmonella typhosa, Salmonella newport, and Serratia macescens (Raintree Nutrition,

Republic of the Philippines

Republic of the Philippines

strawberry with a tang of sour citrus taste.

Republic of the Philippines

Gathering of the Materials

Republic of the Philippines

Results and Discussion

Republic of the Philippines

Republic of the Philippines

Republic of the Philippines

Republic of the Philippines

- Guyabano: Pulpy, sweet and healthy;Malaya;November 23, 2012;p.A12

Republic of the Philippines

Republic of the Philippines

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