Code No: RR212302 Set No.

1
II B.Tech I Semester Supplimentary Examinations, February 2008
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Explain the importance of solid culture media in the development of Microbiology.

[16]

2. What are “Signature sequences” and Polymerase chain reaction? How are they
useful in classification of bacteria? [16]

3. (a) Describe the major classes of fungi.

(b) Explain the differences in major classes in fungi with examples. [8+8]

4. How is energy supplied to drive a conc. gradient across the membrane of the cell?
[16]

5. Describe the methodology for the enrichment of the following bacteria:

(a) Thiobacillus thiooxidans

(b) Rhizobium
(c) Halobacterium
(d) Clostridium. [4×4]

6. Define water activity. Explain how water activity influences the growth of different
microorganisms? [16]

7. (a) Describe the different methods of helozetion?

(b) What is the principle involved in an autoclave? [8+8]

8. (a) What are the tests performed to tests drinking water quality?
(b) What are the common contaminants associated with potable water?
(c) Mention one method to prepare safe drinking water. [4+6+6]

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Code No: RR212302 Set No. 2
II B.Tech I Semester Supplimentary Examinations, February 2008
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe the History and Development of virology. [16]

2. What are “Signature sequences” and Polymerase chain reaction? How are they
useful in classification of bacteria? [16]

3. Describe the general characteristics of Ricketsia. [16]

4. How is energy supplied to drive a conc. gradient across the membrane of the cell?
[16]

5. What is generation time of a given microbial culture ? Derive an equation for

calculating the generation time when a bacterial organism divides by binary fission
in exponential growth. [16]

6. What is water activity ? Explain the importance of water activity on microbial life
and its preservation. [16]

7. (a) How are disinfectants evaluated?

(b) Explain the mechanism of action of anti-microbial agents with suitable exam-
ples? [8+8]

8. What is a Standard operating procedure? Develop an SOP for autoclaving. [16]

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Code No: RR212302 Set No. 3
II B.Tech I Semester Supplimentary Examinations, February 2008
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe the History and Development of virology. [16]

2. Define a “Bacterial species”. Explain the difficulties encountered in classification

of bacteria. [16]

3. Describe the general characteristics of Chlamydia. [16]

4. How is energy supplied to drive a conc. gradient across the membrane of the cell?
[16]

5. What is pure culture of microorganisms ? Explain different methods used in devel-

opment of pure cultures. [16]

6. Define water activity. Explain how water activity influences the growth of different
microorganisms? [16]

7. Distinguish the terms, Disinfection and Sterilization and explain the terms, Disin-
fectant, Sterilant and Antiseptic with suitable examples. [16]

8. How do you minimize the microbial population in a production block in an Indus-

try? [16]

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Code No: RR212302 Set No. 4
II B.Tech I Semester Supplimentary Examinations, February 2008
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe the History and Development of virology. [16]

2. Define a “Bacterial species”. Explain the difficulties encountered in classification
of bacteria. [16]
3. Write short notes on the following.
(a) Dimorphism in Mucor.
(b) Asexual reproduction of Schizo saccharomyces.
(c) Budding in saecharomyces.
(d) Yeast hybridization. [4×4]
4. How is energy supplied to drive a conc. gradient across the membrane of the cell?
[16]
5. Describe the methodology for the enrichment of the following bacteria:
(a) Thiobacillus thiooxidans
(b) Rhizobium
(c) Halobacterium
(d) Clostridium. [4×4]
6. (a) Distinguish between a Primary culture and Stock culture.
(b) Explain how sporulating organisms are preserved using soil.
(c) Discuss about the use of a nutrient agar slant and nutrient agar stab in a
bacteriology laboratory. [6+4+6]
7. List out the most suitable Disinfectant or Antiseptic used for treating the following
materials and justify the selection of a particular chemical
(a) A patch of skin before injection
(b) Drinking water
(c) Laboratory bench top
(d) Forceps [4×4]
8. (a) Mention standard quality control parameters for pharmaceuticals products?
(b) How are they evaluated? [8+8]

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