Code No: RR412302 Set No.

1
IV B.Tech I Semester Supplimentary Examinations, February 2008
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Describe about economic assessment of various proteins via r DNA? [16]

2. Explain the role of Downstream processing and different market sectors in Biotech-
nology? [16]

3. How can you classify Cell disruption methods? Explain in brief about them? [16]

4. Write about the Pressure leaf Filters? [16]

5. Explain about the Basic principles of Membrane separation? [16]

6. Discuss about Colloidal stability of protein solutions? [16]

7. Write short notes on:

(a) Paper chromatography

(b) SDS-PAGE [8+8]

8. Describe about the process of Electro Dialysis? [16]

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Code No: RR412302 Set No. 2
IV B.Tech I Semester Supplimentary Examinations, February 2008
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Explain the different stages in Downstream processing and briefly describe about
techniques employed in each stage? [16]

2. Explain about the Filtration technique employed for several Bioproducts? [16]

3. Write in detail about the Non-Mechanical methods of Cell disruption? [16]

4. What are the various techniques employed in recovery of Bioproducts? Explain in

brief about them? [16]

5. Write short notes on:

(a) Reverse Osmosis

(b) Ultra filtration [8+8]

6. Describe about different techniques employed in Precipitation? [16]

7. Describe the following:

(a) Liquid adsorption chromatography

(b) LLC [8+8]

8. Explain about the process of Dialysis? [16]

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Code No: RR412302 Set No. 3
IV B.Tech I Semester Supplimentary Examinations, February 2008
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Examine the continuing role which economics plays in Bioprocess research,development

and commercialization? [16]

2. Explain about the Filtration technique employed for several Bioproducts? [16]

3. Explain with a neat sketch the working of Homogenizer? [16]

4. What are the various techniques employed in recovery of Bioproducts? Explain in

brief about them? [16]

5. Explain and describe the following membrane separation process RO and Ultra
filtration and clearly delineate between the different process? [16]

6. Describe about different techniques employed in Precipitation? [16]

7. Derive the mass balance for fixed bed adsorption? [16]

8. Explain about the process of Dialysis? [16]

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Code No: RR412302 Set No. 4
IV B.Tech I Semester Supplimentary Examinations, February 2008
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Explain in detail about Bioprocess economics and evaluation of market? [16]

2. Explain the principle of Solvent extraction and its applications with an example?
[16]

3. Explain about working of a bead mill and on what factors does the optimal condi-
tions in a Bead mill depends upon? [16]

4. Write short notes on:

(a) Tubular Centrifuge

(b) Multi chamber centrifuge [8+8]

5. Write short notes on:

(a) Reverse Osmosis

(b) Ultra filtration [8+8]

6. Describe about different techniques employed in Precipitation? [16]

7. Explain in brief about different methods for electrophoresis? [16]

8. Outline the strategies for Crystallization of proteins? [16]

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