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Faculdade de Cincias, Instituto de Oceanografia, Universidade de Lisboa, Campo Grande, 1749-017 Lisbon, Portugal
b
Laboratoire de Cryptogamie, Musum National dHistoire Naturelle, 12, rue Buffon, 75005 Paris, France
Abstract
A taxonomic survey of the microphytobenthic communities was conducted in two intertidal mudflats of the Tagus estuary. These mudflats
are located in different littoral zones of the estuary and had distinct sediment characteristics and salinity range. Sampling was performed
monthly during low tide over a 14-month-period. Sediment cores were collected and brought to the laboratory, where the microalgae were
harvested using the lens-tissue technique under light stimulation. Samples were examined using both light and scanning electron microscopy.
The epipelic pennate diatoms dominated the microphytobenthic assemblages on both mudflats but cyanobacteria and euglenophytes were also
found. Of the 52 taxa identified, 47 were pennate diatoms, five of which seem to be previously unreported for Portugal. The species more
frequently found in the samples were Gyrosigma fasciola, Cylindrotheca cf. signata, Cylindrotheca closterium, Staurophora amphioxys and
Navicula gregaria.
2003 ditions scientifiques et mdicales Elsevier SAS. All rights reserved.
Keywords: Microphytobenthos; Species identification; Tagus estuary
1. Introduction
Microphytobenthic communities are the main primary
producers in intertidal flats of many estuaries. In the case of
the Tagus estuary, they have an estimated primary production
of 11 000 tonnes carbon per year (Brotas and Catarino,
1995), which is about two-fold of the annual primary production of the phytoplankton indicated by (Cabeadas et al.,
2000). The extensive biofilms that develop on the surface of
intertidal sediments are also an active element of the biogeochemistry cycles of sediment-air and sediment-water column interfaces (Cabrita and Brotas, 2000; Dong et al., 2000).
While the taxonomical composition of the phytoplankton
communities of the Tagus estuary has been assessed several
times in the last 30 years ((Moita and Vilarinho, 1998), and
references herein), a thorough taxonomic characterisation of
the benthic microalgae of the Tagus and other Portuguese
estuaries is yet to be achieved. Nevertheless, the overall
structure of the microphytobenthic communities found in the
Tagus intertidal flats and salt marshes, as well as their seasonal and spatial variation, was already established (Brotas
and Plante-Cuny, 1998; Brotas et al., 1995). The former
* Corresponding author.
E-mail address: lourosr@fc.ul.pt (L. Ribeiro).
2003 ditions scientifiques et mdicales Elsevier SAS. All rights reserved.
DOI: 1 0 . 1 0 1 6 / S 1 1 4 6 - 6 0 9 X ( 0 3 ) 0 0 0 1 2 - 2
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Fig. 1. Map of the Tagus estuary showing the sampled stations (Pancas and
Rosrio). Hatched areas, saltmarsh. Thin line, lower limit of intertidal zone.
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Table 1
List of the taxa found on two mudflats of the Tagus estuary. Pancas (P) and
Rosrio (R)
Table 2
Taxa found in more than 2/3 (66%) of the samples of each station and
considering both stations together
Taxa
Bacillariophyta
Achnanthes brevipes Agardh
Amphora coffeaeformis (Agardh) Ktzing
Amphora spp.
Bacillaria paxillifer (O.F. Mller) Hendey
Berkeleya sp.
Caloneis westii W. Smith
Cocconeis sp.
Cosmioneis pusilla (W. Smith) Mann and Stickle
Cyclotella meneghiniana Ktzing
Cylindrotheca closterium Reinmann and Lewin
C. cf. signata Reinmann and Lewin
Cymatopleura solea (Brbisson) W. Smith
Diploneis didyma (Ehrenberg) Cleve
Entomoneis alata (Ehrenberg) Ehrenberg
E. paludosa (W. Smith) Reimer
Epithemia sp.
Frustulia interposita (Lewis) De Toni
Gyrosigma cf. acuminatum (Ktzing) Rabenhorst var. gallica
Grunow
G. (Ktzing) Cleve
G. balticum (Ehrenberg) Rabenhorst
G. distortum (W. Smith) Griffith and Henfrey
G. fasciola (Ehrenberg) Cleve
G. limosum Sterrenburg and Underwood
Gyrosigma sp.
Navicula cryptocephala Ktzing
N. gregaria Donkin
N. phyllepta Ktzing
N. cf. digito-radiata (Gregory) Ralfs
Navicula spp.
Nitzschia acicularis (Ktzing) W. Smith
N. alexandrina (Cholnoky) Lange-Bertalot and Simonsen
N. navicularis (Brbisson) Grunow
N. reversa W. Smith
N. sigma (Ktzing) W. Smith
N. tubicula Grunow
Nitzschia sp.
Petrodyction gemma (Ehrenberg) D.G. Mann
Plagiotropis sp. 1
Plagiotropis sp. 2
Pleurosigma angulatum (Quekett) W. Smith
P. angulatum (Quekett) W. Smith var. aestuarii (Brbisson)
Staurophora amphioxys (Gregory) D.G. Mann
Surirella brebissonii Krammer and Lange-Bertalot
Surirella cf. brightwellii W. Smith
Tryblionella apiculata Gregory
T. gracilis W. Smith
T. hungarica (Grunow) D.G. Mann
Vanheurckia lewisiana (Greville) Brbisson
Pancas
T. gracilis
C. cf. signata
G. limosum
N. gregaria
S. amphioxys
G. attenuatum
G. fasciola
N. sigma
G. distortum
N. naviculares
G. cf. acuminatum
S. cf. brightwellii
Cyanophyta
Oscillatoria spp.
Merismopedia sp.
Euglenophyta
Euglena sp. 1
Euglena sp. 2
Station
P
P
R
R
R
R
R
P
P
P
P
P
P
P
P
P
P
R
R
R
R
R
R
R
R
R
R
P
R
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
P
P
R
R
Rosrio
C. closterium
G. fasciola
C. cf. signata
S. amphioxys
N. gregaria
D. dydima
G. limosum
S. cf. brightwellii
Plagiotropis sp2
Both stations
C. cf. signata
G. fasciola
S. amphioxys
N. gregaria
C. closterium
G. limosum
S. cf. brightwellii
D. dydima
N. sigma
S120
Figs. 29. Diatom SEM micrograph. Fig. 2. G. fasciola, Fig. 3. G. distortum, Fig. 4. G. limosum, Fig. 5. Gyrosigmasp. [cf. acuminatum var. gallica],
Fig. 6. G. attenuatum, Fig. 7. Frustulia interposita, Fig. 8. Staurophora amphioxis, Fig. 9. Navicula gregaria.
dosa (Fig. 16) and Surirella cf. brightwellii (Fig. 17), although not as frequent, did not show any apparent preference
for a given season during the sampling period.
4. Discussion
The microphytobenthic taxa found on both mudflats were
essentially epipelic pennate diatoms, as previously reported
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Figs. 1017. Diatom SEM micrograph. Fig. 10. Cylindrotheca closterium, Fig. 11. Nitzschia sigma, Fig. 12. N. Alexandrina, Fig. 13. N. tubicula,
Fig. 14. Tryblionella apiculata, Fig. 15. T. gracilis, Fig. 16. Entomoneis paludosa, Fig. 17. Surirella sp. [cf. brightwellii].
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References
Admiraal, W., 1984. The ecology of estuarine sediment-inhabiting diatoms.
Progr. Phycol. Res. In: Round, F.E., Chapman, D.J. (Eds.), Biopress,
Bristol, 3, pp. 269332.
Amspoker, M.C., McIntire, C., 1978. Distribution of intertidal diatoms
associated with sediments in Yaquina estuary, Oregon. J. Phycol. 14,
387395.
Bourrely, P., 1981. Les Algues deau douce. Les Algues jaunes et brunnes,
vol. 2. Editions Boube, Paris, pp. 438.
Brotas, V., Catarino, F., 1995. Microphytobenthos primary production of
Tagus estuary intertidal flats (Portugal). Neth. J. Aquat. Ecol 29, 33339.
Brotas, V., Plante-Cuny, M.R., 1998. Spatial and temporal patterns of microphytobenthic taxa of estuarine tidal flats in the Tagus Estuary (Portugal)
using pigment analysis by HPLC. Mar. Ecol. Prog. Ser 171, 4357.
Brotas, V., Cabrita, T., Portugal, A., Serdio, J., Catarino, F., 1995. Spatiotemporal distribution of microphytobenthic biomass in tidal flats of the
Tagus estuary (Portugal). Hydrobiologia 300/301, 93104.
Cabeadas, G., Brogueira, M.J., Cabeadas, L., 2000. Southern Portugal: the
Tagus and Sado estuaries. In: Sheppard, C. (Ed.), Seas at The
Millennium-An Environmental Evaluation. Regional Chapters: Europe,
The Americas and West Africa. Pergamon, Oxford, pp. 151165.
Cabrita, M.T., Brotas, V., 2000. Seasonal variation in denitrification and
dissolved nitrogen fluxes in intertidal sediments of the Tagus estuary,
Portugal. Mar. Ecol. Prog. Ser. 202, 5165.
Cox, E.J., 1996. Identification of Freshwater Diatoms from Live Material.
Chapman and Hall, London, pp. 158.
Dong, L.F., Thornton, D.C.O., Nedwell, D.B., Underwood, G.J.C., 2000.
Denitrification in sediments of the River Colne estuary, England. Mar.
Ecol. Prog. Ser. 203, 109122.
Eaton, J.W., Moss, B., 1966. The estimation of number and pigment content
in epipelic algal populations. Limnol. Oceanogr 11, 584596.
Germain, H., 1980. Flore des Diatomes. Editions Boube, Paris, pp. 444.
Hopkins, J.T., 1964. A study of the diatoms of the Ouse estuary, SussexII.
The ecology of the mudflat diatom flora. J. Mar. Biol. Assoc. UK 44,
333384.
Krammer, K., Lange-Bertalot, H., 1986. Bacillariophyceae. In: Ettl, H.,
Gerloff, J., Heynig, H., Mollenhauer, D. (Eds.), Naviculaceae, Swasserflora von Mitteleuropa, 1. Gustav Fisher Verlag, Stuttgart, pp. 876.
Krammer, K., Lange-Bertalot, H., 1988. Bacillariophyceae. In: Ettl, H.,
Gerloff, J., Heynig, H., Mollenhauer, D. (Eds.), Bacillariaceae, Epithemiaceae, Surirellaceae, Swasserflora von Mitteleuropa, 2. Gustav
Fisher Verlag, Stuttgart, pp. 878.
McIntire, C.D., Moore, W.W., 1977. Marine littoral diatoms: ecological
considerations. In: Dietrich, W. (Ed.), The Biology of Diatoms, Botanical Monograph 13. Blackwell Scientific Publications, London,
pp. 333365.
Moita, M.T., Vilarinho, M.G., 1998. Checklist of phytoplankton species off
Portugal: 70 years (1929-1998) of studies. Portugaliae Acta Biol., Sr. B,
Sist. 18, 550.
Moore, W.W., McIntire, C.D., 1977. Spatial and seasonal distribution of
littoral diatoms in Yaquina estuary, Oregon, USA. Bot. Mar. 20, 99109.
Patrick, R., Reimer, C.W., 1966. The Diatoms of the United States. Acad.
Nat. Sci. Phil. vol. 1, 688 (Monograph 13).
S123
Round, F.E., Crawford, R.M., Mann, D.G., 1990. The Diatoms: Biology and
Morphology of the Genera. Cambridge University Press, Cambridge,
pp. 747.
Sousa e Silva, E., Assis, M.E., Sampayo, M.A., 1969. Primary productivity
in the Tagus and Sado estuaries from May 1967 to May 1968. Notas e
Estudos Inst. Biol. Marit. 37, 131.
Tolomio, C., Moro, I., Mocschin, E., Valandro, A., 1999. Rsultats prliminaires sur les diatomes benthiques de substrats meubles deans Lagune
de Venise, Italie (mars 1994-janvier 1995). Diatom Res. 14, 367397.
Underwood, G.J.C., 1994. Seasonal and spatial variation in epipelic diatom
assemblages in the Severn Estuary. Diatom Res 9, 451472.
Underwood, G.J.C., Phillips, J., Saunders, K., 1998. Distribution of estuarine benthic diatom species along salinity and nutrient gradients. Eur. J.
Phycol 33, 173183.
Witkowski, A., Lange-Bertalot, H., Metzeltin, D., 2000. Diatom flora of
marine coasts I. In: Lange-Bertalot, H. (Ed.), Iconographia Diatomologica, 7. Ganter Verlag, Ruggell, pp. 925.
Zong, Y., Horton, B., 1998. Diatom zones across intertidal flats and coastal
saltmarshes in Britain. Diatom Res 13, 375394.