Professional Documents
Culture Documents
NMR instruments use a static magnetic field to make nuclear spins orient in the
magnetic field.
gauss (0.15 to 21 tesla). Field strengths correlate with the proton frequency of the
instruments here at the UNSW NMR Facility:
Horizontal distance of
1
H Frequency (MHz)
Bruker 300
Bruker 500
Bruker 600
*vertical distances are larger
Field (Tesla, T)
7
11.4
14
Fields at the
2)
The cryogens which may cause burns when as liquids they contact with the
outside the magnet; therefore, no movable metal objects should be taken within the
~2 to 5 metres (3 to 5 gauss) radius of the instrument. Small, sharp metal objects
flying towards the magnet are highly dangerous. Larger objects are troublesome to
scrape off the magnet, and can seriously damage the magnet. Very large objects
have been known to break the cryogen dewar(s) in the magnets resulting in the
release of the cryogens contained in the magnet (See Quench). Magnetic objects
Page 1 of 7
Laboratory Induction and Authorisation Form Date Effective: 01/01/2007
Uncontrolled document when printed Current Version: 2.0, 01/03/2007
flying towards the magnet pose threat of serious injury/death to any person in the
way.
Metal belt buckles, steel tipped shoes, paper clips, hair pins, and any other
metal on the person may be strongly attracted when close to the magnet.
spectrometers.
Demand-type
Persons fitted with metallic implants and prostheses should not get closer
certain types of credit cards and mobile phones. Keep those items more than 2.5 m
away from the center of magnet.
NMR workers should spend no longer than reasonable necessary within the
Hands and arms etc. should not be placed into the bore of the magnet
(widebore VI 300).
Cryogenic Liquids
In the unlikely event of the magnet quenching (the rapid release of gaseous
cryogens from the cryostat into the room) or of a cryogenic container failure, up to
100 m3 of helium gas may evolve over a period of several minutes. Although inert,
lighter than air and non-toxic, there could be a risk of asphyxiation in a confined
space. Personnel should evacuate the NMR laboratory IMMEDIATELY in such a
situation. A quench warranting evacuation would be obvious by the noise of the
escaping gas and clouds of vapor. Similarly, rupture of a liquid nitrogen container
resulting in a large volume of nitrogen gas being released also requires evacuation of
the laboratory.
indicated by cryogen level sensors may result in a magnet quench with possible
magnet damage.
warning messages are displayed on the BSMS instrument panel, immediately inform
the NMR facility staff.
Electrical Hazards
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Laboratory Induction and Authorisation Form Date Effective: 01/01/2007
Uncontrolled document when printed Current Version: 2.0, 01/03/2007
Electrical/RF hazards:
electrocution, the equipment circuit breakers will be turned off by NMR Facility staff.
Sample Care
Breakage and Accidents
ALL accidents inside the probe (e.g. broken tubes) MUST be reported to
All users MUST dispose of their own samples in their own laboratories. Short
term storage of labeled NMR tubes in the spectrometer rooms (e.g. in the fridge) is
permitted for samples that do not present significant safety risk.
Fire
When transferring liquid nitrogen or helium, the following steps should be observed to
avoid accidents:
Gloves, eye protection, and closed shoes (NOT STEEL CAPPED) must be
worn.
No access to the pit (DMX 600) is permitted during nitrogen fills as exit is
-269 degrees C or 4.17 degrees K. Liquid helium will boil at 4.22 degrees K. Any
disruption to the temperature or loss of the vacuum will cause the liquid helium within
the magnet to "boil off" causing an immediate and sudden loss of the magnetic field.
This event is referred to as a "quench". When this occurs, the helium will expand at
a rate of approximately 760 to 1. This means that for every litre of liquid helium, 760
litres of gas would be produced. This marked expansion in volume causes the gas to
rush out of the magnet into the spectrometer room. Magnets in use hold more 100
litres of liquid helium and liquid nitrogen (DMX 600).
1)
2)
3)
What is a cryogen? Name the two liquid cryogens used in the magnets and
4)
5)
What is a quench?
6)
7)
8)
9)
How much deuterated solvent should be used in a 5mm NMR tube (if
10)
How long should an NMR tube be? (if submitting a sample on an auto-sampler
11)
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Laboratory Induction and Authorisation Form Date Effective: 01/01/2007
Uncontrolled document when printed Current Version: 2.0, 01/03/2007
12)
Lab coats, food, drinks and plastic/latex gloves should not enter the NMR Lab
(B41).
True
13)
False
b.
c. 2 x Emergency Exits
e. 1 x Safety shower
f. 1 x Eyewash
Store
Room
Tesla
Scientific
Officer
Manager
Maya
Gyro
ESR
Flip
Page 6 of 7
Tech.
Laboratory Induction Officers
and Authorisation Form Date Effective: 01/01/2007
Uncontrolled document when printed Current Version: 2.0, 01/03/2007
Entrance
Hertz
Gauss
approval of their supervisor(s) and access fees will be charged for all booked
instrument time. Users must not interfere with any of the NMR instruments if they
have not booked the time or are not licensed to use the instrument by NMR staff.
Users must also agree not to provide access to the NMR Facility to unregistered
users without the specific permission of the NMR staff. Users are not allowed to train
other users, whether they are registered or not, unless permitted to do so by NMR
staff. Users are not permitted to undertake any work that is not directly related to
their project. Users agree to accurately complete all record keeping required by the
NMR to monitor the use of instruments e.g. log books and booking systems. Users
agree to report all faults or irregularities in the NMR instruments to a NMR staff
member immediately they are known. Samples are the responsibility of the DIY user
and the NMR Facility does not accept any responsibility for loss or damage of
samples left in the facility. Data is the responsibility of the DIY user and the NMR
Facility does not accept responsibility for loss or damage of data that has not been
removed/copied/archived. Any work that is published or publicly presented, where all
or a part of the work was undertaken in the NMR Facility, should acknowledge the
role of the UNSW NMR Facility in providing access or contributing to or assisting in
the work.
Failure to comply will result in the loss of access to the NMR Facility.
I _______________________________, have undertaken laboratory induction. I
agree to abide by all the above requirements as outlined by the Laboratory Manager.
Signature
_______________________________
Date
_______________________________
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Laboratory Induction and Authorisation Form Date Effective: 01/01/2007
Uncontrolled document when printed Current Version: 2.0, 01/03/2007