Professional Documents
Culture Documents
Department of Animal Ecology and Ecophysiology, Institute for Water and Wetland Research, Radboud
University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands; 2Biofouling and Biofilm Processes
Section, Water and Steam Chemistry Division, BARC Facilities, Kalpakkam, 603 102 Tamil Nadu, India;
3
KEMA Power Generation and Sustainables, P.O. Box 9035, 6800 ET Arnhem, The Netherlands; *Author for
correspondence (e-mail: s.rajagopal@science.ru.nl; fax: +31-24-355-3450)
Received 29 June 2005; accepted in revised form 25 January 2006
Key words: Biofouling, Green mussels, Growth rate, Invasion, Perna viridis, Physiology, Reproduction,
Review
Abstract
The green mussel Perna viridis has been receiving a lot of attention from workers working in the research
areas of intertidal ecology, aquaculture, pollution monitoring, biofouling, zoogeography and invasion
biology. P. viridis is a remarkable species in terms of its ability to reach very high biomass levels, to
withstand environmental fluctuations, to concentrate a variety of organic and inorganic environmental
pollutants, to colonise artificial marine habitats and to invade new geographic territories. This review
collates data available on salient aspects of the distribution, biology and ecology of P. viridis. It is argued
that the remarkable success of P. viridis as an invasive species basically stems from its long larval duration,
fast growth rate, high fecundity, early maturity, high productivity and ability to withstand fluctuating
environmental conditions (temperature, salinity, water turbidity and pollutants). Relevant aspects of the
data are compared with the data available for a similar species Perna perna, which too is an invasive species,
but to a more limited extent.
Introduction
The green mussel Perna viridis (L.) (Syn. Mytilus
viridis, Mytilus smaragdinus, Mytilus opalus,
Chloromya viridis, Chloromya smaragdinus) (Siddall 1980) is the tropical and subtropical counterpart of the relatively better studied blue mussel
Mytilus edulis L., a mussel that is widely distributed in the higher latitudes (Bayne 1976). Both
species are dominant in many rocky littoral and
shallow sublittoral ecosystems and often contribute significantly to the productivity of coastal
benthos (Vakily 1989; Hickman 1992). The green
mussel has been attracting lot of attention not only
heavy metals and other pollutants from surrounding waters has been used by many
researchers to employ these mussels as sentinel
organisms (Sukasem and Tabucanon 1993;
Sudaryanto et al. 2002; Monirith et al. 2003).
Recently, P. viridis has also received lot of attention as an invading species at new localities
(Agard et al. 1992; Kazuhiro and Sekiguchi 2000;
Hicks et al. 2001; Ingrao et al. 2001).
The Genus Perna
Classication
Mollusca
Bivalvia
Pteriomorphia
Mytiloida
Mytilidae Ranesque, 1815
Perna Retzius, 1788
Within the genus Perna usually four species are
distinguished, viz., the green mussel Perna viridis
(Linnaeus, 1758), the brown mussel Perna perna
(Linnaeus, 1758), the green-lipped mussel Perna
canaliculus (Gmelin, 1791) and the Spanish
mussel (Mediterranean green mussel) Perna picta
(Born, 1780) (Figure 1). P. canaliculus and
P. picta are geographically restricted to New
Zealand and the Mediterranean Sea, respectively.
However, the other two species enjoy wide distribution in several parts of the world (Siddall
1980; Vakily 1989; Hicks et al. 2001) and often
co-exist (Rajagopal et al. 1997, 1991b, 1998b).
They have been vigorously invading new geographical regions (Hicks and Tunnell 1993). The
introduction to new areas has been probably
caused by international shipping, either as adults
attached to ship hulls or as larvae in ballast water
tanks (Hicks and Tunnell 1995). P. perna was
first introduced to the Caribbean Sea for culture
purpose, which led to its successful establishment
in nearby areas (Hicks and Tunnell 1993).
P. viridis can potentially dominate its benthic
habitat and displace other Perna species
(Rajagopal et al. 1997).
Distribution
P. viridis is native of the Indo-Pacific region, primarily distributed along the Indian and the
Southeast Asian coasts. They generally inhabit
marine intertidal, subtidal and estuarine environments with high salinity. P. viridis is a characteristic species of the fauna of midlittoral and
sublittoral zones, where it often constitutes dense
populations. It is gregarious and its large numbers
cluster together with the aid of its well-developed
byssal apparatus. The mussels form a thick carpetlike growth on rocky surfaces and submerged
structures like wharves, pilings, breakwaters and
buoys (Huang et al. 1983; Rao 1990) and they play
a very important role in rocky shore ecosystems. These mussels attach to surfaces by byssus
threads colonizing submerged rocks, wood, concrete, metal, old submerged logs, boats, PVC
pipes, ropes, muddy sea bottoms and even seagrass
beds and mangrove prop roots (Vakily 1989;
Agard et al. 1992; Rajagopal et al. 1997). P. perna
is distributed along the southern tip of India, Sri
Lanka (Kuriakose and Nair 1976), the coasts of
the African continent up to Morocco (Siddall
1980; Shafee 1989) and in some parts of South
Figure 1. Photograph showing the four species of bivalve genus Perna: (1) the green mussel Perna viridis (Linnaeus, 1758) (Indonesia,
Madura island), (2) the green-lipped mussel Perna canaliculus (Gmelin, 1791) (New Zealand, Wellington, Lyall Bay) (3) the Spanish
mussel or Mediterranean green mussel Perna picta (Born, 1780) (Morocco, Immouzer) and (4) the brown mussel Perna perna
(Linnaeus, 1758) (South Africa, Durban). All shells are from the collection of the National Natural History Museum Naturalis, Leiden
(RMNH), The Netherlands.
Table 1. Diagnostic characters of green mussel, Perna viridis and the brown mussel, Perna perna (modified after Kuriakose 1980 and
Rajagopal 1997).
Conchological features
Perna viridis
Perna perna
Shells*
Highly concave
Curved
Arcuate
Yellowish-green or light green
Inner fold of the posterior ventral
mantle margin: thin, extensible, smooth,
tentacles or papillae absent
Thick, broad, extends slightly to the ventral border
Two small on the left valve and one on the right valve
Absent
Deeply impressed
Large and situated at the posterior base of the foot
Thick, white and highly pitted
Two short and thick bundles
Mouth oval and wide; passage into
the mantle cavity small;
rectum and posterior adductor
not visible through the opening
*Similarities.
America including Venezuela, Brazil and Argentina (Siddall 1980; Chung and Acuna 1981).
The native range of P. viridis stretches across the
Indo-Pacific encompassing the Persian Gulf, India, Malaysia, Papua New Guinea and the South
Pacific islands, and north to Japan (Sivalingam
1977; Siddall 1980; Vakily 1989; Cheung 1993).
According to Siddall (1980), P. viridis has the
potential to increase its geographical distribution
by Island hopping, a kind of step-wise larval
dispersal. Though it is not a part of the native
fauna of northern South America (Siddall 1980), it
has been recorded from Trinidad in the mid 1990s
(Agard et al. 1992). P. viridis further moved
southward to the Gulf of Paria, using the prevailing currents and is thought to have dispersed to
Venezuela by currents (Agard et al. 1992) as well
as by human activities (Rylander et al. 1996). The
mussel larvae are believed to have been transported via ballast water to Tampa Bay estuary,
Florida, where it is now well established (Tampa
Bay Estuary Program 2000).
Reproduction
In P. viridis, the sexes are separate, but males and
females are not distinguishable by external morphology. The animals become sexually mature at
15 30 mm shell length (i.e., about 2 3 months
old) (Siddall 1980; Vakily 1989). Males are distinguishable by the milky white gonads; females have
bright orange brick red coloured gonads (Rajagopal 1991). Gonadal tissues of P. viridis appear to
be invading the whole of body, filling the mantle
lobes, mesosoma and outer surface of the digestive
glands. Figure 2 shows the histological features of
ripe testes and ovaries in P. viridis. Fertilization is
external. The spawning behaviour of P. viridis
differs considerably (Vakily 1989; Rajagopal et al.
1998a). Spawning is initiated by either sex, resulting in the release of streams of gametes into the
water (Stephen and Shetty 1981). Spawning may
also be induced by the presence of other spawning
individuals in the area or also by a drop in salinity
(Stephen and Shetty 1981). Typically, in estuarine
Figure 2. Photomicrographs of male and female gonads at different stages in sexual cycle of Perna viridis (100). (a c) indicate
developing/re-developing stages; (d) shows ripe gonads; (e) shows spawning in progress and (f) shows spent/resting gonads.
stages in the reproductive cycle of P. viridis (Figure 2). The stages are described below:
Stage 1 (developing/redeveloping gonad): This
includes (a) immature gonads, characterised by a
thin, transparent and colourless mantle; hardly
distinguishable sexes; follicles distinguishable as
small opaque areas and sometimes not visible;
gametogenesis initiated, but ripe gametes not yet
visible and (b) developing gonads, specimens with
a relatively thick mantle; male and female gonads
are distinguishable; creamy white in case of males
and orange in case of females; follicles larger and
denser; gametogenesis is still progressing but
follicles contains mainly ripe gametes.
Stage 2 (ripe gonad): Mantle much thicker than in
stage 1; male mantle milky white in colour and
female bright orange brick red in colour; entirely
packed with follicles and little connective tissue
seen between follicles; ova are compacted into
polygonal configurations; male gonad is distended
with morphologically ripe sperm.
Stage 3 (spawning gonad): Gonad tissues become
dull; reduction in sperm density; appearance of
empty spaces along with full follicles; the follicles
are about one third full of ripe gametes.
Stage 4 (spent/resting gonad): This stage includes
animals that have completed spawning; mantle
semi-transparent; follicle wall ruptures and few
signs of residual gametes.
Gonad index (GI) can also be determined based on
the method described by King et al. (1989):
GI Number in each stage
numerical ranking of that stage 100=
Number of animals in the sample
where, gonad index of the sample may vary from 0
(all mussels resting) to 300 (all mussels ripe or
redeveloping). According to Seed (1976), the ranks
are allotted as follows: 0 resting; 1 immature
and/or spent; 2 developing and/or spawning;
3 ripe and/or redeveloping.
Breeding seasons
Rajagopal et al. (1998b) reported that gonadal
development and spawning activity of P. viridis
at Kalpakkam (east coast of India) were linked
Figure 3. Seasonal variations of different gonadal maturity stages (in percent) and gonad index of Perna viridis and seasonal occurrence of mussel larvae in coastal waters of Kalpakkam on the east coast of India (modified after Rajagopal et al. 1998b).
Figure 4. Monthly variations of spat settlement of Perna viridis at different depths in Kalpakkam coastal waters and on the intake
screens of cooling conduits of a power station (from Rajagopal et al. 1998b).
Growth rate
P. viridis is capable of high growth rates, especially
under favourable conditions (Vakily 1989).
Rajagopal et al. (1998b) reported that on the
southeast coast of India, the mussel could reach
119 mm shell length in the first year and 152 mm
in the second year (Figure 6). However, such rates
were observed at higher than normal flow conditions i.e., on the screen walls of a power station
intake. Interestingly, the mean value in the first
Figure 5. (a) Seasonal variations of wet weight per m)2of Perna viridis and other fouling species at different depths on experimental
concrete surfaces and (b) Relationship between total weight of fouling settlement and weight of Perna viridis in coastal waters of
Kalpakkam on the east coast of India (redrawn from Rajagopal et al. 1997).
Figure 6. Growth of Perna viridis in Kalpakkam after settlement in coastal waters and on the intake screens of cooling
conduits of a power station. Data are presented as meanSD
(n=27 30).
year of their existence from Tolo Harbour, another polluted harbour in Hong Kong. Compared
with values reported by Vakily (1989) and Tomalin
(1995), the growth rate recorded at Kalpakkam,
southeast coast of India (Rajagopal et al. 1998b),
stands apart, being 10 mm month)1 a rate that is
the highest ever reported for P. viridis.
Environmental tolerance
One of the main reasons for the extraordinary
invasive ability of the green mussel is its tolerance
to a wide range of environmental conditions. The
mussel is quite hardy and individuals have been
reported to do well in articial seawater for more
than 6 months (Nishida et al. 2003). P. viridis
tolerates a temperature range of 15 32.5 C
without much problem (Rajagopal et al. 1995a).
The species can survive a temperature of 39 C for
about 200 min (Figure 7). The thermal tolerance
of P. viridis becomes very conspicuous compared
with that of P. perna (Figure 7). P. viridis thrives
well at winter water temperatures as low as 12 C
(Benson et al. 2001). Segnini de Bravo (2003)
reported that P. viridis has a higher degree of
adaptability to salinity changes and, therefore, a
greater potential for aquaculture than P. perna
(Romero and Moreira 1980; Salomao et al. 1980).
Sivalingam (1977) reported that the normal fluctuation in salinity (27 33&) observed in estuarine
habitats was well within the lower and upper
tolerance limits of P. viridis. However, it can
Filtration
Excretion
Nitrogenous compounds, end products of protein
and amino acid catabolism, are the major excretory products in mussels. Among the nitrogenous
wastes, ammonia is the major excretory product
(Bayne et al. 1976). Masilamoni et al. (2001)
presented data on excretion of nitrogen and
phosphorus by different size groups of P. viridis
at different salinities (15, 20, 25, 30 and 34&).
Salinity was found to influence the rate of excretion. Ammonia excretion in different size groups
of mussels increased as the salinity was lowered
up to 25&. On further decrease of salinity, there
was a decrease in the excretion rate, which stopped completely at 15&. Apart from ammonia,
animals excrete nitrite and nitrate as part of a
mechanism for detoxification of ammonia and
maintenance of internal ionic stability. Masilamoni et al. (2001) reported that nitrite and nitrate
excretion in P. viridis showed increase with
decrease in salinity. Phosphate excretion, on the
other hand, decreased significantly with decrease
in salinity.
Release of nitrogenous (ammonia, nitrite and
nitrate) and phosphorus (phosphate) wastes by
large mussel communities may signicantly aect
the nutrient dynamics of the receiving water body
(Kuenzler 1961; Richard and Dankers 1988).
There have been reports of mussel activities
(especially, selective filtering and nutrient excretion) promoting algal blooms in bays and lakes
(Vanderploeg et al. 2001). Discharge of condenser
effluents from cooling water systems of coastal
power plants that harbour massive mussel communities (e.g., see Venugopalan et al. 1991) into
inshore areas need to be studied from this point of
view (Masilamoni et al. 2001). In view of the
dramatic population increase of P. viridis in many
coastal localities, bays and harbours, it is suggested that nutrient loading by mussels be given
due attention by researchers.
Cultivation
Pest and predators
According to Bayne (1976), natural enemies of
mussels fall into four categories: predators, competitors, parasites and shell borers. Algae, hydroids, free and tubiculous polychaetes, barnacles,
amphipods and ascidians are important pests
which colonise the outer surface of shell valves of
P. viridis and compete for space. The main predators include crabs, fishes, starfish and octopus
(Rao 1990). The mud crab Scylla serrata is considered a major predator of P. viridis, while among
fishes snapper, silver bream and black tail have
been mentioned (Vakily 1989). However, there are
not many studies on the effects of predation on
P. viridis. Cheung et al. (2004a, b) studied the
defensive responses of P. viridis on being
challenged with two predators, the muricid gastropod, Thais clavigera, and the portunid crab,
Thalamita danae. They observed that responses of
the mussels were predator-specific. Mussels raised
in the presence of crabs developed thicker shell at
the umbo and lip margin, while those raised in the
presence of gastropods had a thicker shell lip.
Predators also were shown to choose their prey;
jagopal et al. 1991a, b). Among them green mussels were the most dominant species, in terms of
biomass. The green mussels have proven to be a
successful fouling species in a variety of maritime
and industrial environments. Their widespread
distribution and their ability to attach to different
surfaces even at high flow rates and to make use of
water flow to achieve fast growth rates and high
population densities, make them highly suited to
colonise cooling water systems (Rajagopal 1997).
Their characteristic ability to survive extremes of
environmental conditions (salinity 0 64& and
temperature 6 37.5 C), thrive well in turbid
coastal waters (Morton 1987) and survive under
prolonged biocide dosing that gets rid of most of
their competitors (Rajagopal et al. 2003a), has
contributed to their extraordinary success as fouling species. In fact, P. viridis can withstand harsh
environmental conditions better than its temperate
counterpart Mytilus edulis (Morton 1987) or its coexisting species P. perna (Rajagopal 1997).
Uncontrolled growth of the green mussels can
create problems for power plants using seawater as
the condenser coolant. With continuous ow of
water that brings in sucient quantity of food
and oxygen, apart from fresh stock of larvae, the
exposed surfaces like intake tunnel, screens, pipes
and culverts are quickly colonised by young mussels (Rajagopal et al. 1996a). Economic aspects of
fouling-induced effects on electric power plants
can be very significant, such as reduced flow of
cooling water for steam condensation, causing
increased condenser backpressure and belowoptimum performance (Rajagopal 1997). Live
mussels or empty shells can cause mechanical
damage to pumps and block the flow of water in
condenser tubes and reduce the heat transfer efficiency (Neitzel et al. 1984; Rajagopal et al. 1994).
Mussel shells can also cause accelerated corrosion
of the condenser tubes (Fischer et al. 1984). There
are reports of extensive growth of green mussels
in the condenser cooling systems of power stations
in India (Rajagopal et al. 1991a, b).
Over the years, P. viridis has been able to
expand its geographical territory into new areas.
Today, it is recognised as a potential fouling
organisms in countries such as Malaysia, Hong
Kong, Japan, China, USA (Florida), Trinidad and
Venezuela, making it a truly global player (Morton
1987; Agard et al. 1992; Kazuhiro and Sekiguchi
2000; Rajagopal et al. 2003b). Fouling biomass
Figure 13. (a) Seasonal variations of total, shell and meat weights of Perna viridis on ropes in Edaiyur backwaters, east coast of India
and (b) Changes in total production, biomass and density estimates of Perna viridis in Edaiyur backwaters east coast of India (redrawn
from Rajagopal et al. 1998a).
Mussel control
The type of fouling control measures adopted by
an industry depends on the system being fouled.
Antifouling paints have been the method of choice
for combating biofouling on ship hulls. However,
paints have only limited service life and require
Mussels as biomonitors
The use of bivalves, especially mussels, as sentinel
organisms (the Mussel Watch concept) for marine pollutants has been proposed by Goldberg
(1986) and Bayne (1989). This method served to
monitor the pollution in the world oceans in the
1980s and 90s (Goldberg and Bertine 2000). Using
this method, mussels have been used as bioindicators to study contamination of coastal waters by
a variety of organic and inorganic pollutants,
including heavy metals, organochlorines and
PCBs. Biomonitoring of persistent organochlorines in the coastal marine environments of the
Asia pacific region, carried out using P. viridis, can
be found in recent work by Monirith et al. (2003).
Sukasem and Tabucanon (1993) used P. viridis to
monitor heavy metals such as zinc, manganese,
Holmes M.J., Ming Teo S.L. and Khoo H.W. 1999. Detection
of diarrhetic shellfish poisoning toxins from tropical shellfish
using liquid chromatography-selected reaction monitoring
mass spectrometry. Nat. Toxins 7: 361 364.
Hopkins T.L. 1977. Zooplankton distribution in surface waters
of Tampa bay, Florida. Bull. Mar. Sci. 27: 467 478.
Huang Z.G., Lee S.Y. and Mak P.M.S. 1983. The distribution
and population structure of Perna viridis (Bivalvia: Mytilacea) in Hong Kong waters. In: Morton B. and Dudgeon D.
(eds), Malacofauna of Hong Kong and Southern China,
Hong Kong University Press, Hong Kong, pp. 465 471.
Ingrao D.A., Mikkelsen P.M. and Hicks D.W. 2001. Another
introduced marine mollusk in the Gulf of Mexico: the IndoPacific green mussel, Perna viridis, in Tampa Bay, Florida.
J. Shellfish Res. 20: 13 19.
Jenner H.A., Whitehouse J.W., Taylor C.J.L. and Khalanski
M. 1998. Cooling Water Management in European Power
Stations: Biology and Control. Hydroecologie Appliquee
1 2, Electricite de France, Chatou, Paris, pp. 1 225.
Jorgensen C.B. 1983. Patterns of uptake of dissolved aminoacids in mussels (Mytilus edulis). Mar. Biol. 73: 177 182.
Jose B. and Deepthi T.R. 2005. Green mussel Perna viridis, a
new host for the pea crab Pinnotheres placunae along the
Malabar coast, Kerala. Curr. Sci. 89: 1090 1091.
Kaehler S. and McQuaid C.D. 1999. Lethal and sub-lethal
effects of phototrophic endoliths attacking the shell of the
intertidal mussel Perna perna. Mar. Biol. 135: 497 503.
Kautsky N. 1982. Quantitative studies on gonad cycle, fecundity, reproductive output and recruitment in a Baltic Mytilus
edulis population. Mar. Biol. 68: 143 160.
Kazuhiro H. and Sekiguchi H. 2000. Perna mussels introduced
into Ise and Mikawa Bays, Central Japan. Sessile Org. 17:
1 11.
Ke C. and Wang W.X. 2002. Trace metal ingestion and
assimilation by the green mussel Perna viridis in a phytoplankton and sediment mixture. Mar. Biol. 140: 327 335.
Kimmerer W.J., Gartside E. and Orsi J.J. 1994. Predation by an
introduced clam as the likely cause of substantial declines in
zooplankton of San Francisco Bay. Mar. Ecol. Prog. Ser.
113: 81 93.
King P.A., McGrath D. and Gosling E.M. 1989. Reproduction
and settlement of Mytilus edulis on an exposed rocky shore in
Galway bay, west coast of Ireland. J. Mar. Biol. Assoc. UK
69: 355 365.
Koya M.S. and Mohandas A. 1982. Rare occurrence of a sexually mature adult digenetic trematode in the green mussel,
Perna viridis. Linn. J. Helminth. 56: 41 43.
Kreeger D.A. and Newell R.I.E. 1996. Ingestion and assimilation of carbon from cellulolytic bacteria and heterotrophic
flagellates by the mussels Geukensia demissa and Mytilus
edulis (Bivalvia, Mollusca). Aquat. Microb. Ecol. 11: 205
214.
Kuenzler E.J. 1961. Phosphorus budget of a mussel population.
Limnol. Oceanogr. 6: 400 415.
Kuriakose P.S. 1980. Mussels (Mytilidae: Genus Perna) of the
Indian coast. In: Coastal Aquaculture: Mussel Farming,
Progress and Prospects. CMFRI Bulletin 29, Indian Council
of Agricultural Research, Cochin, India, pp. 1 5.
Kuriakose P.S. and Nair N.B. 1976. The genus Perna along the
coasts of India with description of a new species, Perna
indica. Aquat. Biol. 1: 25 36.