Exam1 S14

CH 369 Exam 1
February 6, 2014
Name: __________________________________________ eID ________________
Helpful Equations:
degrees K = 273 + C
Section I (32 points): 1 point each

1. Which of the following would be the strongest acid?
A) formic acid, pK=3.75
B) succinic acid, a diprotic acid with pK=4.21 and 5.64
C) acetic acid, pK=4.76
D) ammonium ion, pK=9.25
E) cannot be determined from the given information
2. Which of the following amino acids has a nonpolar side chain?
A) Ser
D) Asn
B) Val
E) none of these choices
C) Lys
3. Bone and tendons contain _____ while hair and nails contain _____.
A) collagen; keratin
D) collagen; actin
B) actin; keratin
E) tubulin; keratin
C) tubulin; collagen
4. -helices and -sheets comprise ___ structure.
A) primary
B) secondary
C) tertiary
D) quaternary
5. Which amino acid is critical for crosslinking of keratin fibers?

A) Ser
B) Lys
C) His
D) Cys
E) Gln
6. At a pH above its pKa, the phenolic group of tyrosine is ___.

A) protonated and neutral
D) deprotonated and negatively charged
B) protonated and positively charged
C) deprotonated and neutral
7. Which of the 20 standard amino acids is optically inactive?
A) Arg
B) Pro
C) Ile
D) Gly
8. Which of the following would have the lowest melting point?

A) NH3
B) CH4
Scrambled version A
Page 1
C) H2 O
E) Cys
9. What is the conjugate acid of H2 PO4 -?

A) HPO4 2B) H2 PO4
C) H3 PO4
D) PO4 3E) none of the above
10. Of the following amino acids, which contains an alcohol?

A.
B.
C.
A) A
B) B
C) C
D.
D) D
E) all of the above
11. Which of the following would be a proper designation of the deoxy position on DNA?
A) 3' (3 prime)
B) 3
C) 2' (2 prime)
D) 2
E) 1
12. If the following two reactions were coupled, what would be the G for the overall exergonic reaction?
ATP + H 2O ADP + P i
G = -31 kJ/mol
Glucose + Pi glucose-1-phosphate + H2O
A) -52 kJ/mol
B) -10 kJ/mol
C) 10 kJ/mol
G = 21 kJ/mol
D) 52 kJ/mol
E) none of the above
13. The most important buffering system for maintaining proper blood pH is:
A) the charges on the amino acids
B) the bicarbonate buffer system of CO2 , carbonic acid, and bicarbonate
C) phosphate groups of serum phosphoproteins
D) none of the above
E) all of the above
14. A protein that consists of a single polypeptide chain will lack which level of protein structure?
A) Primary
B) Secondary
C) Tertiary
D) Quaternary
E) Pentanary
Scrambled version A
Page 2
15. What level of protein structure is characterized by the three dimensional structure of an entire polypeptide
including all amino acid side chains?
A) primary
D) quaternary
B) secondary
C) tertiary
16. The Bohr effect describes a lowered O2 -affinity of Hb when the pH _____.
A) increases
B) decreases
17. The higher the dielectric constant, the more ________ the compound.
A) polar
D) nonpolar
B) negatively charged
E) oxidized
C) positively charged
18. Which RNA molecules are involved in the process of translation?
A) mRNA
B) tRNA
C) rRNA
D) A and C
19. Which of the following is a simple statement of Chargaffs rules?
A) A+G = C+T
B) A = C
C) C = T
20. Heme is considered a _____.
A) catalyst
B) prosthetic group
C) coenzyme
E) A, B and C
D) G = A
D) cofactor
21. Which of the following is the most abundant structural element in the human body?
A) nitrogen
D) phosphorous
B) carbon
C) oxygen
22. What bonding interactions are broken during DNA denaturation?
A) Covalent bonds
D) B and C
B) Hydrogen bonds
E) A, B and C
C) van der Waals interactions
23. The tertiary structures of myglobin, -hemoglobin and -hemoglobin are nearly identical and are therefore
nearly identical in primary structure.
A) True
B) False
24. Which of the following assembles by association of multiple protofilaments?
A) myosin
B) actin
C) tubulin
D) keratin
Scrambled version A
Page 3
E) collagen
25. What term describes the process of converting the information found in DNA into the sequence of a
protein?
A) replication
D) expression
B) transcription
C) translation
26. Which of the two proteins below has a longer neck region?
A) myosin
B) keratin
C) kinesin
D) collagen
E) actin
27. Which of the following is an example of the hydrophobic effect?

A) the lipid membrane of cells and organelles
B) protein folding that places hydrophobic amino acids in the interior of the protein
C) the separation of salad dressing
D) oil sheens seen on the ocean following an oil spill
E) all of the above
28. At physiological pH, all amino acids have at least ___ charged groups.
A) 0
D) 3
B) 1
E) cannot be determined
C) 2
29. The replication of DNA is made possible by the presence of _____ strands in the double helix of DNA.
A) antiparallel
D) genomic
B) hydrogen bonded
C) complementary
30. Which of the following amino acids has a thiol group in its side chain?
A) Ser
B) Asn
C) Cys
D) Tyr
31. Which of the following molecules contains the most oxidized form of carbon?
A) acetaldehyde
D) ethylene
B) ethanol
E) carbon dioxide
C) acetic acid
32. In a muscle cell, what is the name for the bundles of myosin tails?
A) cytoskeleton
D) thin filaments
B) thick filaments
C) intermediate filaments
Section II (50 points): 2 points each

33. Which of the following statements regarding RNA is FALSE?
A) The ribose in RNA contains an O atom that the ribose in DNA lacks.
B) RNA does not form a 2-stranded double helix like DNA.
C) Since RNA does not form a double helix, it tends to be linear in shape.
D) RNA can form Watson-Crick base pairs with DNA.
Scrambled version A
Page 4
E) Met
34. Would DNA-binding proteins be more likely to associate with the major or the minor groove?
A) Major groove
B) Minor groove
35. Identify the amino acid structure below.
A) H
B) I
C) R
D) D
E) V
36. At pH ___, the pentapeptide Ala-Glu-His-Val-Cys would contain two positively charged groups and one
negatively charged group.
A) 1
B) 3.5
C) 5
D) 7
E) 9
37. If a phosphate buffer (pK 6.82) was formulated such that its pH was 7.3, it would be best suited to buffer
against _____. If instead, it was formulated such that its pH was 6.3, it would be best suited to buffer
against _____.
A) acid; base
B) acid; acid
C) base; acid
D) base; base
E) a buffer with a pH that far from the pK would not be an effective buffer
38. Which of the biopolymers is correctly paired with its major function?
A) protein: information encoding
D) polysaccharide: energy storage
B) nucleic acids: energy storage
C) lipids: information encoding
39. Each kinesin head contains a binding site for _____ and a binding site for _____.
A) tubulin; GTP
D) actin; ATP
B) another kinesin molecule; GTP
E) tubulin; ATP
C) myosin; ATP
40. Which allosteric effector has the greatest ability to stabilize the deoxy state of hemoglobin?
A) BPG
D) H+
B) CO2
E) all of the above have equal ability
C) O2
Scrambled version A
Page 5
41. A plot of the binding of oxygen to myoglobin as a function of pO2 gives a _____ shape; a similar plot for
hemoglobin gives a _____ shape.
A) sigmoidal; sigmoidal
D) hyperbolic; hyperbolic
B) sigmoidal; hyperbolic
E) hyperbolic; exponential
C) hyperbolic; sigmoidal
42. If the following mixture of proteins was applied to a size-exclusion chromatography column, what would
be the order of elution?
Proteins with molecular weights (kDa = kiloDalton = kg/mol): myoglobin (17.7 kDa) , hemoglobin (64.5
kDa) , lysozyme (14.3 kDa) and triose phosphate isomerase (57.4 kDa)
A)
B)
C)
D)
E)
lysozyme, myoglobin, triose phosphate isomerase, hemoglobin

triose phosphate isomerase, hemoglobin, lysozyme, myoglobin
hemoglobin, myoglobin, lysozyme, triose phosphate isomerase
hemoglobin, triose phosphate isomerase, myoglobin, lysozyme,
cannot be determined
43. You are involved in the purification of Protein X with a pI of 6.0 using a buffer with a pH of 7.5. What
type of resin would you choose to ensure initial binding of Protein X?
A) DEAE (pK ~10)
B) CM (pK ~4)
44. Which of the following processes are involved in gene expression?
A) Replication
B) Transcription
C) Translation
D) Both B and C
E) A, B and C
45. The pK of CH3 CH2 NH3 + is 10.7. Would the pK of FCH2 CH2 NH3 + be higher or lower?
A) Higher
B) Lower
46. Which of the following methods of amino acid sequence determination requires an unmodified amino
terminus?
A) Mass spectroscopy
D) Edman sequencing
B) Ion exchange
E) X-ray crystallography
C) SDS-PAGE
47. Which of the following is an example of a conservative amino acid substitution?
A) Ile Leu
B) Ser Glu
C) Phe Tyr
D) Asp Gln
E) Arg Cys
48. What is the fractional saturation (p5 0 = 2.8 torr) of myoglobin when pO2 = 15 torr?
A) 0.65
D) 1.23
B) 0.58
E) None of the above are correct
C) 0.84
Scrambled version A
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49. What would be the resulting pH if one drop (0.05 ml) of 1.0 M HCl was added to one liter of pure water
(assume pH 7.0)?
A) 2.7
D) 7.0 (there would be no significant change)
B) 4.3
E) 9.7
C) 5.0
50. What is the net charge of the heptapeptide His-Ala-Cys-Asp-Ser-Glu-Asn at pH 7?
A) -2
D) +1
B) -3
C) 0
51. Do Chargaffs rules apply to RNA as well as to DNA?
A) Yes
B) No
52. For a globular protein that is found in the cytosol, ___ would most likely be found in the proteins interior
while ___ would most likely be found on the surface.
A) Val; Phe
D) Thr; His
B) Leu; Ile
C) Leu; Ser
53. Which of the following would represent an increase in entropy?
A) Ice freezes
D) Sewing a garment
B) Traffic congestion on highway
E) Filing papers
C) Dismissal of class when bell rings
54. Which of the following atoms would be considered a hydrogen bond donor in an -helix?
A) a
B) b
C) c
D) d
55. What is the pH of a solution that contains one part acetic acid and three parts sodium acetate? The pK for
acetic acid is 4.76.
A) 5.24
B) 5.06
C) 4.46
D) 4.28
Scrambled version A
Page 7
56. Which of the following fibers is correctly paired with the protein that forms the fiber?
A) microfilaments: actin
D) intermediate filaments: keratin
B) microtubule: tubulin
E) all of the above
C) extracellular support fibers: collagen
57. Which of the following explains the attractive forces between hydrophobic molecules in an aqueous
solution?
A) in an aqueous environment, London dispersion forces between hydrophobic molecules become
stronger
B) in an aqueous environment, London dispersion forces between hydrophobic molecules and water
become stronger
C) since nonpolar molecules do not form hydrogen bonds with hydrogen bonds with water, they can form
hydrogen bonds with other nonpolar molecules
D) there is no increase in attractive forces between nonpolar molecules in an aqueous environment
Section III (18 points): 3 points each

58. The oxygen-binding curves for normal hemaglobin (Hb A) and a mutant hemoglobin (Hb Great Lakes) are
shown in the figure below. Which of the two hemoglobins: (i) has a higher affinity for oxygen when pO2 =
20 torr; and (ii) is most efficient at delivering oxygen from arterial blood (pO2 = 75 torr) to active muscle
(pO2 = 20 torr)?
A) (i) Hb A; (ii) Hb A
B) (i) Hb A; (ii) Hb Great Lakes
C) (i) Hb Great Lakes; (ii) Hb A
D) (i) same affinity; (ii) Hb A

E) (i) Hb Great Lakes; (ii) same efficiency
Scrambled version A
Page 8
59. Biochemists sometimes link a recombinant (modified) protein to green fluorescent protein (GFP). The
fluorophore in GFP is a derivative of three consecutive amino acids that undergo cyclization of the
polypeptide chain and an oxidation. Using the structure below, identify: (i) the three amino acid residues;
and (ii) the bond that results from oxidation.
A) Met-Tyr-Ala; c
B) Ser-Tyr-Gly; a
C) Ser-Phe-Gly; c
D) Ser-Tyr-Gly; b
E) Met-Tyr-Ala; a
60. Based on the following Tm values, what can be said about these two DNA molecules: Strand A = 80C;
Strand B = 100C?
A) Strand A has a higher percentage of adenine bases.
B) Strand B has a higher percentage of cytosine bases.
C) Strand B has stronger stacking interactions.
D) All of the above
61. Rank the solubilities of the following amino acids in water at pH 7: W, R, S, V, T.
A) V < W < T < S < R
D) W < V < T < S < R
B) W < V < S < T < R
E) R < T < S < V < W
C) V < W < S < T < R
62. Urea (NH2 CONH2 ) dissolves readily in water. The beaker containing the dissolved compound is cold to
the touch. What conclusions can you make about the sign of the (i) enthalpy change; and (ii) entropy
change for this process?
A) + H; + S
D) - H; + S
B) - H; - S
E) Cannot be determined
C) + H; - S
63. A solution is made by mixing 25 mL of a stock solution of 2.0 M K2 HPO4 (pK 6.82) and 50 mL of a stock
solution of 2.0 M KH2 PO4 . The solution is diluted to a final volume of 200 mL. What is the final pH of
the solution?
A) 7.12
B) 6.82
C) 6.52
D) 6.22
E) 7.42
Scrambled version A
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Answer Key - Exam1_S14

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Scrambled version A
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Scrambled version A
Page 11
CH 369 Exam 1
February 6, 2014
Name: __________________________________________ eID ________________
Helpful Equations:
degrees K = 273 + C

A) antiparallel
D) genomic
B) hydrogen bonded
C) complementary
A) primary
B) secondary
C) tertiary
D) quaternary

A) myosin
B) actin
C) tubulin
D) keratin
E) collagen
A) nitrogen
D) phosphorous
B) carbon
C) oxygen
A) Arg
B) Pro
C) Ile
D) Gly
A) cytoskeleton
D) thin filaments
B) thick filaments
Scrambled version B
Page 1
E) Cys

A.
A) A
B) B
C) C
B.
C.
D.
D) D
E) all of the above

A) primary
D) quaternary
B) secondary
C) tertiary
A) 0
D) 3
B) 1
C) 2
A) Ser
B) Asn
C) Cys
D) Tyr
E) Met
E) all of the above
A) Primary
B) Secondary
C) Tertiary
D) Quaternary
E) Pentanary
Scrambled version B
Page 2
A) NH3
B) CH4
C) H2 O
A) True
B) False
A) catalyst
B) prosthetic group
C) coenzyme
D) cofactor

A) Ser
B) Lys
C) His
D) Cys
E) Gln
A) Ser
D) Asn
B) Val
C) Lys
A) myosin
B) keratin
C) kinesin
D) collagen
E) actin

E) all of the above
D) collagen; actin
B) actin; keratin
E) tubulin; keratin
Scrambled version B
Page 3
G = -31 kJ/mol
A) -52 kJ/mol
B) -10 kJ/mol
C) 10 kJ/mol
G = 21 kJ/mol
D) 52 kJ/mol
A) increases
B) decreases
A) 3' (3 prime)
B) 3
C) 2' (2 prime)
D) 2
E) 1
protein?
A) replication
D) expression
B) transcription
C) translation
A) HPO4 2B) H2 PO4
C) H3 PO4
A) acetaldehyde
D) ethylene
B) ethanol
E) carbon dioxide
C) acetic acid
A) Covalent bonds
D) B and C
B) Hydrogen bonds
E) A, B and C
A) mRNA
B) tRNA
C) rRNA
D) A and C
A) polar
D) nonpolar
E) oxidized
Scrambled version B
Page 4
E) A, B and C

A) A+G = C+T
B) A = C
C) C = T
D) G = A

A) Major groove
B) Minor groove
A)
B)
C)
D)
E)

terminus?
D) Edman sequencing
B) Ion exchange
C) SDS-PAGE
against _____.
A) acid; base
B) acid; acid
C) base; acid
D) base; base
A) Yes
B) No
A) Replication
B) Transcription
C) Translation
D) Both B and C
Scrambled version B
Page 5
E) A, B and C
A) 1
B) 3.5
C) 5
D) 7
E) 9
A) tubulin; GTP
D) actin; ATP
E) tubulin; ATP
C) myosin; ATP
A) -2
D) +1
B) -3
C) 0
E) all of the above
(assume pH 7.0)?
A) 2.7
B) 4.3
E) 9.7
C) 5.0
A) 0.65
D) 1.23
B) 0.58
C) 0.84
A) DEAE (pK ~10)
B) CM (pK ~4)
A) Ice freezes
D) Sewing a garment
E) Filing papers
Scrambled version B
Page 6
solution?
stronger
become stronger
A) 5.24
B) 5.06
C) 4.46
D) 4.28
A) H
B) I
C) R
D) D

A) Ile Leu
B) Ser Glu
C) Phe Tyr
D) Asp Gln
E) V
E) Arg Cys
A) Val; Phe
D) Thr; His
B) Leu; Ile
C) Leu; Ser
Scrambled version B
Page 7
A) a
B) b
C) c
D) d
A) BPG
D) H+
B) CO2
C) O2
A) Higher
B) Lower

Strand B = 100C?
D) All of the above
A) + H; + S
D) - H; + S
B) - H; - S
C) + H; - S
A) V < W < T < S < R
D) W < V < T < S < R
B) W < V < S < T < R
E) R < T < S < V < W
C) V < W < S < T < R
Scrambled version B
Page 8
(pO2 = 20 torr)?

the solution?
A) 7.12
B) 6.82
C) 6.52
D) 6.22
E) 7.42
A) Met-Tyr-Ala; c
B) Ser-Tyr-Gly; a
C) Ser-Phe-Gly; c
D) Ser-Tyr-Gly; b
E) Met-Tyr-Ala; a
Scrambled version B
Page 9

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Scrambled version B
Page 10
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Scrambled version B
Page 11
CH 369 Exam 1
February 6, 2014
Name: __________________________________________ eID ________________
Helpful Equations:
degrees K = 273 + C

A) cytoskeleton
D) thin filaments
B) thick filaments
A) Primary
B) Secondary
C) Tertiary
D) Quaternary
E) Pentanary
A) increases
B) decreases
A) Arg
B) Pro
C) Ile
D) Gly
E) Cys
A) primary
D) quaternary
B) secondary
C) tertiary
A) True
B) False
A) Covalent bonds
D) B and C
B) Hydrogen bonds
E) A, B and C
A) A+G = C+T
B) A = C
C) C = T
D) G = A
A) 3' (3 prime)
B) 3
C) 2' (2 prime)
D) 2
E) 1
Scrambled version C
Page 1

A) HPO4 2B) H2 PO4
C) H3 PO4
A) Ser
D) Asn
B) Val
C) Lys
A) NH3
B) CH4
A) Ser
B) Lys
C) His
C) H2 O
D) Cys
E) Gln
A) polar
D) nonpolar
E) oxidized
protein?
A) replication
D) expression
B) transcription
C) translation
A) primary
B) secondary
C) tertiary
D) quaternary
A) myosin
B) keratin
C) kinesin
D) collagen

A) mRNA
B) tRNA
C) rRNA
D) A and C
Scrambled version C
Page 2
E) actin
E) A, B and C

E) all of the above
E) all of the above
A) 0
D) 3
B) 1
C) 2
D) collagen; actin
B) actin; keratin
E) tubulin; keratin
A) antiparallel
D) genomic
B) hydrogen bonded
C) complementary
A) acetaldehyde
D) ethylene
B) ethanol
E) carbon dioxide
C) acetic acid
A) Ser
B) Asn
C) Cys
D) Tyr
Scrambled version C
Page 3
E) Met

A.
B.
C.
A) A
B) B
C) C
D.
D) D
E) all of the above

A) catalyst
B) prosthetic group
C) coenzyme
D) cofactor
A) nitrogen
D) phosphorous
B) carbon
C) oxygen
A) myosin
B) actin
C) tubulin
D) keratin
E) collagen
G = -31 kJ/mol
A) -52 kJ/mol
B) -10 kJ/mol
C) 10 kJ/mol
G = 21 kJ/mol
D) 52 kJ/mol

A) Yes
B) No
A) 1
B) 3.5
C) 5
D) 7
E) 9
Scrambled version C
Page 4

A) -2
D) +1
B) -3
C) 0
terminus?
D) Edman sequencing
B) Ion exchange
C) SDS-PAGE
A) Higher
B) Lower
A) a
B) b
C) c
D) d
A) H
B) I
C) R
Scrambled version C
D) D
Page 5
E) V
A) BPG
D) H+
B) CO2
C) O2
A) 5.24
B) 5.06
C) 4.46
D) 4.28
A) Major groove
B) Minor groove
A) 0.65
D) 1.23
B) 0.58
C) 0.84
(assume pH 7.0)?
A) 2.7
B) 4.3
E) 9.7
C) 5.0
A) Ice freezes
D) Sewing a garment
E) Filing papers
A) Ile Leu
B) Ser Glu
C) Phe Tyr
D) Asp Gln
E) Arg Cys
A) Val; Phe
D) Thr; His
B) Leu; Ile
C) Leu; Ser
A) Replication
B) Transcription
C) Translation
D) Both B and C
Scrambled version C
Page 6
E) A, B and C
E) all of the above
A)
B)
C)
D)
E)

A) DEAE (pK ~10)
B) CM (pK ~4)
A) tubulin; GTP
D) actin; ATP
E) tubulin; ATP
C) myosin; ATP
solution?
stronger
become stronger
Scrambled version C
Page 7
against _____.
A) acid; base
B) acid; acid
C) base; acid
D) base; base

(pO2 = 20 torr)?

Strand B = 100C?
D) All of the above
Scrambled version C
Page 8

A) V < W < T < S < R
D) W < V < T < S < R
B) W < V < S < T < R
E) R < T < S < V < W
C) V < W < S < T < R
A) Met-Tyr-Ala; c
B) Ser-Tyr-Gly; a
C) Ser-Phe-Gly; c
D) Ser-Tyr-Gly; b
E) Met-Tyr-Ala; a
the solution?
A) 7.12
B) 6.82
C) 6.52
D) 6.22
E) 7.42
A) + H; + S
D) - H; + S
B) - H; - S
C) + H; - S
Scrambled version C
Page 9

1.
2.
3.
4.
5.
6.
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B
D
B
D
C
B
D
A
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A
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B
B
D
A
D
B
A
E
E
D
B
C
A
C
E
C
A
B
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B
A B
B
A
D
D
B
A
B
A
A
A
C
Scrambled version C
Page 10
45.
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B
C
A
C
D
E
D
A
C
E
D
C
A
C
D
D
B
C
A
Scrambled version C
Page 11

Exam1 S14

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Exam1 S14

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CH 369 Exam 1

Section I (32 points): 1 point each

5. Which amino acid is critical for crosslinking of keratin fibers?

6. At a pH above its pKa, the phenolic group of tyrosine is ___.

8. Which of the following would have the lowest melting point?

9. What is the conjugate acid of H2 PO4 -?

D) PO4 3E) none of the above

10. Of the following amino acids, which contains an alcohol?

27. Which of the following is an example of the hydrophobic effect?

Section II (50 points): 2 points each

lysozyme, myoglobin, triose phosphate isomerase, hemoglobin

Section III (18 points): 3 points each

D) (i) same affinity; (ii) Hb A

Answer Key - Exam1_S14

Section I (32 points): 1 point each

3. Which of the following assembles by association of multiple protofilaments?

7. Of the following amino acids, which contains an alcohol?

8. Which of the following would be the strongest acid?

17. Which amino acid is critical for crosslinking of keratin fibers?

20. Which of the following is an example of the hydrophobic effect?

D) PO4 3E) none of the above

32. Which of the following is a simple statement of Chargaffs rules?

Section II (50 points): 2 points each

lysozyme, myoglobin, triose phosphate isomerase, hemoglobin

52. Which of the following is an example of a conservative amino acid substitution?

Section III (18 points): 3 points each

D) (i) same affinity; (ii) Hb A

Answer Key - Exam1_S14

Section I (32 points): 1 point each

10. Which of the following would be the strongest acid?

D) PO4 3E) none of the above

19. Which RNA molecules are involved in the process of translation?

20. Which of the following is an example of the hydrophobic effect?

28. Of the following amino acids, which contains an alcohol?

29. Heme is considered a _____.

Section II (50 points): 2 points each

35. What is the net charge of the heptapeptide His-Ala-Cys-Asp-Ser-Glu-Asn at pH 7?

40. Identify the amino acid structure below.

lysozyme, myoglobin, triose phosphate isomerase, hemoglobin

Section III (18 points): 3 points each

D) (i) same affinity; (ii) Hb A

60. Rank the solubilities of the following amino acids in water at pH 7: W, R, S, V, T.

Answer Key - Exam1_S14

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