Bio Rad Water

Bio-Rad Laboratories Water Testing
Water Safety
Guide for Bio-Rad Products in Water Testing

2011
Water Safety
Guide for Bio-Rad Products in Water Testing
Contents
Part 1 Quality Indicators Testing
Total flora
E. coli EN ISO miniaturized MPN standard
E. coli Automated alternative method: XplOrer64 System
E. coli/Coliforms NF EN ISO standards
E. coli/Coliforms RAPIDE.coli 2 alternative method
Enterococci EN ISO miniaturized MPN standard
Enterococci Automated alternative method: XplOrer64 System
Enterococci EN ISO standard
10
Enterococci RAPIDEnterococcus alternative method
11
Part 2 Pathogens Testing

Sulfite-Reducing Anaerobes (Clostridium) NF EN ISO standards
12-13
Legionella spp. & Legionella pneumophila NF EN ISO standards methods
14-15
Legionella spp. and Legionella pneumophila Extraction for real-time PCR method 16
Legionella spp. and Legionella pneumophila Alternative real-time PCR method
17
Legionella detection in aerosols with CIP10-M Apparatus Bio-Rad solution
18
Legionella detection in biofilms, Amoeba Bio-Rad technical notes
19
Pseudomonas aeruginosa EN ISO standards
20
!
New Pseudomonas aeruginosa RAPIDP.aeruginosa alternative method
21
Salmonella spp. ISO standard
22-23
Staphylococcus aureus NF standard
24-25
This booklet is only an indication provided by Bio-Rad for the use of Bio-Rad products.
It is not a contractual document.
Quality Indicators Testing
Total flora
EN ISO 6222 (07/1999)
Water quality
Enumeration of culturable micro-organisms.
Colony-count by inoculation
in a nutrient agar culture medium.
Pour-Plate method
Water sample
(preparation)
Ordering Information
Catalog # Description
PCA (Plate Count Agar)

without Dextrose/Agar
355-4437
356-4474
200 ml x 6 bottles
500 g
PCA without Dextrose/Agar
68 hrs
22C
Reading/
enumeration
44 hrs
36C
Reading/
enumeration
Guide for Bio-Rad Products in Water Testing |
| 3
Catalog #
Description
355-4282
18 ml x 60 tubes

DSM + Bromophenol Blue
E. coli - EN ISO miniaturized MPN standard
DSM (Dehydrated Special

Microplates Diluent)
355-3784
100 g
EDS (Distilled Water for Microplates)

355-4283
18 ml x 60 tubes
Fluoplate 2 Software
EN ISO 9308-3 (03/1999)

Water quality
Detection and enumeration of Escherichia coli
and coliforms bacteria
Part 3: Miniaturized method (Most Probable
Number) for the detection and enumeration
of E. coli in surface and residual water
Water sample
(dilutions in DSM or EDS)
Data management software for the statistical

estimation of MPN and its confidence interval
Please contact your local representative
MUG/EC E. coli Microplates Kit

355-3785

355-3782

25 opaque white plates

with stickers
25 transparent plates
with stickers
MUG/EC (microplates)
Long-Wave UV Lamp, Compact Portable

166-0500

1 lamp (366 nm, requires

4 AA batteries)
Portable UV Observation Chamber

(without Wood Lamp)
355-0717
1 chamber
Square Petri dishes

355-0304
36-72 hrs
44C
Reading
( = 366 nm - UV light)
UV lamp
120 mm x 252 units
UV Observation Chamber
(Wood Lamp)
355-0718
1 UV tube (366 nm)
Key Benefits
Statistical control (Xhi2 test)
of the input values
Creation of a database easily
exportable into Excel* or Access*
Multi-language software and reports:
english, french, spanish, italian and
german
*Excel is a trademark of Microsoft
*Access is a trademark of Access Systems
4 |
| Guide for Bio-Rad Products in Water Testing
Calculation and interpretation

E. coli - Automated alternative method:

XplOrer64 System
Catalog #
Automated real-time and continuous quantification

of Escherichia coli in bathing
!
(coastal sea and inland water) and waste waters
New
NF Validation according to Protocol for the
Validation of a commercial method versus a reference
method in the water microbiology field (revision 0),
Attestation No BRD 07/18 - 11/09
Samples poor in
suspended matters (SM)
100 ml protocol
Samples with high

SM level
10 ml protocol
Non filterable water

samples
1 ml protocol
Bathing water (sea, inland)

Treated waste water
Drinking waters
Treated or untreated
waste water
Untreated
waste water
Water sample
filtration
Water sample
filtration
Pipetting of
1 ml of water
sample
Description
XplOrer64 System
Full Configuration (220/110V)
XplOrer64 System for up to 64 samples,
delivered with computer, UPS, color
printer, CheckNSafe temperature control
cells, cleaning box and 4 racks
XplOrer64 System
Configuration w/o PC (220V)
XplOrer64 System 220V for up to 64
samples, CheckNSafe temperature
control cells, cleaning box and 4 racks
XplOrer64 System
CheckNSafe E.coli Kit

355-4720
Membrane inserting
into
CheckNSafe
E.coli
Membrane inserting
into
CheckNSafe
E.coli
Direct inoculation
CheckNSafe
E.coli
9 ml x 60 tests
CheckNSafe Screw Caps

359-3457
Sterile caps (1 bag of 30 units)
Key Benefits
!
New
Automated detection or quantification with

XplOrer64 System
Total E. coli
analysis cycle:
- bathing water: 8 hrs

- waste water: 12 hrs
- drinking water: 12 hrs
Automated real-time results analysis

and interpretation with
XplOrer64 Software
First alternative method certified

NF VALIDATION for bathing and
waste water monitoring
Easy-to-use and automated
method to quantify E. coli in
real-time, without confirmation
Random access for up to 64
sample analyzed simultaneously
Fast Results:
2,000 E. coli/100 ml detected in
4 hrs 20 min
500 E. coli/100 ml detected
within 5 hrs
Automatic expression of results
according to 76/160/EEC or
2006/7/EC European Directive
Accurate alternative method
compared to miniaturized MUG/
EC Microplates
| 5
Catalog #
Description
BCP (Bromocresol Purple)

Lactose/Broth
355-3414
10 ml x 25 tubes
E. coli/Coliforms - NF EN ISO standards

EN ISO 9308-1 (09/2000)
Water quality
Detection and enumeration of
Escherichia coli and coliforms bacteria
Part 1: Membrane-filtration method
BGBLB (Brilliant Green Bile

Lactose Broth)
357-8024
10 ml x 25 tubes
Water sample filtration
Water sample (dilutions)
Membrane
BCP Lactose Broth

or
Tryptose Lauryl Sulfate
Broth
Kovacs Reagent
355-5313
NF T90-413 (10/1985)
Water testing
coliforms and thermotolerant
coliforms
General method by culture in
liquid media (MPN)
15 ml x 2 bottles
Oxidase Disks
355-3832
30 disks
Peptone Water (without Indole)/Broth

355-4180
356-4334
9 ml x 25 tubes
500 g
Schubert/Broth
355-5694
356-9634
8 ml x 25 tubes
500 g
Tergitol 7 (0.2%)/Supplement
356-2632
356-2635
50 ml x 1 bottle
5 ml x 1 vial
24-48 hrs
30C
Standard test
TTC Tergitol 7
Rapid test
TSA
Trouble and gas+
TTC (0.05%)/Supplement
356-2652
356-2655
50 ml x 1 bottle
5 ml x 1 vial
TTC Tergitol 7/Agar - Base Medium

355-4687
356-4454
200 ml x 6 bottles
500 g
TTC Tergitol 7/Agar - Complete Medium

356-3706
356-3980
90 mm x 20 dishes
200 ml x 6 bottles
8 ml x 25 slanted tubes
500 g
Tryptophane/Broth
355-4194
3 ml x 25 tubes
Tryptose Lauryl Sulfate/Broth

356-4774
6 |
(10) yellow colonies

Transfer the
with yellow area membrane on TBA
55 mm x 10 dishes
55 mm x 100 dishes
Trypto-Casein-Soy (TSA)/Agar
356-3884
355-5947
355-5944
356-4554
4-5 hrs
36C
21 hrs
(+21 hrs)
36C
500 g
19-20 hrs
44C
TSA Tryptophane Membrane on

broth
10 tubes
indole paper
10 tubes
+ UV
21 hrs
36C
Oxidase test
Oxidase
disks
21 hrs
44C
Indole test
Kovacs
reagent
Red
colonies
10-30
min
BGBLB
BGBLB
or
Schubert
48 hrs
37C
Trouble
gas +
Total
coliforms
24-48 hrs
44C
Trouble
gas +
Thermotolerant
coliforms
Indole test
(Kovacs reagent
in Schubert)
E. coli

E. coli/Coliforms - Alternative method:
RAPIDE.coli 2 for Water Testing
RAPIDE.coli 2 for Water Testing
Chromogenic agar medium for the direct detection and
enumeration of E. coli and coliforms, by membrane-filtration
!
NF VALIDATION according to Protocol for the
New
validation of a commercial method versus a reference
method in the water microbiology field (revision 0),
Attestation No BRD 07/20 - 03/11
Catalog #
Description
RAPIDE.coli 2 Kit for Water

Testing
355-5296
6 bottles + 6 vials of sup.
!
New
RAPIDE.coli 2/Agar
for Water Testing
356-3982
55 mm x 20 dishes
+
Membrane
24 hrs
36C
for coliforms and E. coli
RAPID' E.coli 2 Agar for Water Testing
Key Benefits
Enumeration of typical colonies

Green colonies = Coliforms
Grey blue to violet colonies = E. coli
Certified by NF VALIDATION
One single plate for simultaneous
E. coli and coliforms testing
Complete results within 18 4 hrs
at just one temperature of 36C
Good colony coloration contrast
for a rapid reading
No confirmation step required
| 7
Catalog #
Enterococci - EN ISO miniaturized MPN standard
Description
DSM + Bromophenol Blue

355-4282
18 ml x 60 tubes
DSM (Dehydrated Special

Microplates Diluent)
355-3784
EN ISO 7899-1 (03/1999)

Water quality
Detection and enumeration of Enterococci
in surface and waste water
Miniaturized method (Most Probable Number)
by inoculation in liquid medium
100 g
EDS (Distilled Water for Microplates)

355-4283
18 ml x 60 tubes
Water sample
(dilutions in DSM or EDS)
Data management software for the statistical

estimation of MPN and its confidence interval
MUD/SF Enterococci Microplates Kit

355-3786

355-3783

25 opaque white plates

with stickers
25 transparent plates
with stickers
MUD/SF (microplates)

166-0500


4 AA batteries)

(without Wood Lamp)
355-0717
1 chamber
Square Petri dishes

355-0304
36-72 hrs
44C
Reading
UV lamp
120 mm x 252 units
(Wood Lamp)
355-0718
1 UV tube (366 nm)
Key Benefits
Statistical control (Xhi2 test)
of the input values
Creation of a database easily
exportable into Excel* or Access*
Multi-language software and reports:
english, french, spanish, italian and
german
*Excel is a trademark of Microsoft
*Access is a trademark of Access Systems
8 |
Calculation and interpretation

Enterococci - Automated alternative method:

XplOrer64 System
New
Catalog # Description
Automated real-time and continuous quantification

of intestinal Enterococci in bathing water (coastal sea
and inland water) and waste waters
NF Validation according to Protocol for the

Validation of a commercial method versus a
reference method in the water microbiology field
(revision 0), Attestation No BRD 08/19 - 11/09
Samples poor in
suspended matters (SM)
100 ml protocol
Samples with high

SM level
10 ml protocol
Non filterable water

samples
1 ml protocol
Bathing water (sea, inland)

Treated waste water
Treated or untreated
waste water
Untreated
waste water
Water sample
filtration
Water sample
filtration
Pipetting of
1 ml of water
sample
XplOrer64 System
Full Configuration (220/110V)
XplOrer64 System for up to 64 samples,
delivered with computer, UPS, color
printer, CheckNSafe temperature control
cells, cleaning box and 4 racks
XplOrer64 System
XplOrer64 System
CheckNSafe Enterococci Kit

355-4721
Membrane inserting
into
CheckNSafe
Enterococci
Membrane inserting
into
CheckNSafe
Enterococci
Direct inoculation
CheckNSafe
Enterococci
10 ml x 60 tests
CheckNSafe Screw Caps

359-3458
Sterile caps (1 bag of 30 units)
Key Benefits
New
! Automated detection or quantification with

XplOrer64 System
Total Enterococci
analysis cycle:
- bathing water: 12.5 hrs
- waste water: 16 hrs
Automated real-time results analysis

and interpretation with
XplOrer64 Software
First alternative method certified

NF VALIDATION for bathing and
waste water monitoring
Easy-to-use and automated
method to quantify in real-time,
without confirmation
Random access for up to 64
samples analyzed simultaneously
Fast Results:
400 Enterococci/100 ml detected
in 8 hrs 30 min
250 Enterococci/100 ml detected
within 9 hrs
Automatic expression of results
according to 76/160/EEC or
2006/7/EC European Directive
Accurate alternative method
compared to miniaturized MUD/SF
microplates
| 9
Catalog #
Enterococci - EN ISO standard
Description
BEA/Agar
356-3994
355-5829
356-4184
90 mm x 20 dishes
100 ml x 6 bottles
500 g
NF EN ISO 7899-2 (08/2000)

Water quality
Detection and enumeration
of intestinal Enterococci
TTC (0.2%)/Supplement
356-2665
5 ml x 1 vial
Slanetz and Bartley/Agar

Base Medium
355-4819
356-4934
100 ml x 6 bottles
500 g
Slanetz and Bartley/Agar

Complete Medium
356-3716
356-3981
55 mm x 10 dishes
55 mm x 100 dishes
Membrane
Slanetz & Bartley
44 hrs
36C
Reading
Transfer the membrane on BEA
2 hrs
44C
Reading/enumeration
10 |

Enterococci - RAPIDEnterococcus
alternative method
RAPIDEnterococcus
Chromogenic agar for
the direct detection and enumeration
of intestinal Enterococci,
by membrane-filtration
Catalog #
Description
RAPIDEnterococcus/Agar
355-4409
100 ml x 6 bottles
Membrane
RAPIDEnterococcus
8-10 ml per plate
( = 55 mm)
44 hrs 4 hrs
44C
Enumeration of typical colonies

Blue colonies = Enterococcus
Key Benefits
Direct identification and
enumeration of Enterococci
Ready-to-use medium
Save time and materiel

| 11
Catalog #
Description
Columbia/Agar
356-4674
500 g
Meat-Liver Sulfite Iron/Agar

Complete Medium
355-4770
355-4777
355-4794
356-9654
100 ml x 6 bottles
20 ml x 25 tubes
7.5 ml x 25 tubes
500 g
TSC without D-Cycloserine/Agar

Base Medium
355-4419
356-9644
Sulfite-Reducing Anaerobes (Clostridium) - NF

NF T90-415 (10/1985)
Water testing
Detection and enumeration of spores of
sulfito-reducing anaerobic bacteria
and of Clostridium
General method by incorporation of agar
in deep tubes
Water sample (preparation)
100 ml x 6 bottles
500 g
10 min
80C
Meat-Liver Sulfite Iron

Anaerobic
conditions
24 hrs
37C
Reading
Anaerobic
conditions
Meat-Liver Sulfite Iron Agar
12 |
48 hrs
37C
Reading/enumeration
Pathogens Testing
EN ISO standards
NF EN 26461-1 (07/1993)
Water quality
spores of sulfito-reducing anaerobic
micro-organisms (Clostridia)
ISO/DIS 6461-2 (06/2005)

(ISO 6461-2:1986 review)
Water quality
Clostridium perfringens

15 min
60C
15 min
75C
DRCM
Anaerobic
conditions
44 hrs
37C
Reading/enumeration
Membrane
TSC without D-Cycloserine
Anaerobic
conditions
15 min
60C
Reading/enumeration
Confirmation
(Columbia Agar, Nutrient Agar 2.1%)
| 13
Catalog #
Legionella spp. & Legionella pneumophila NF EN
Description
BCYE Biplate/Agar
356-3718
90 mm x 20 dishes
ISO 11731 (05/1998):

Water quality
Detection and enumeration of Legionella
BCYE with L-Cysteine/Agar

356-3720
90 mm x 20 dishes
BCYE without L-Cysteine/Agar

356-3722
90 mm x 20 dishes
Columbia Agar + Horse Blood/Agar

356-3804
90 mm x 20 dishes
Direct sample analysis
GVPC/Agar
356-3717
356-3719
90 mm x 20 dishes
90 mm x 100 dishes
Legionella pneumophila Latex Kit

356-2790
50 tests
Legionella pneumophila and

Legionella Species Latex Kit
356-2795
50 tests
MonoFluo Kit
Legionella pneumophila
353-2514
0.2 ml
GVPC
0.2 ml 1/10
for dirty water
GVPC
24 tests
Nutrient/Agar (For Water Testing)

356-3786
90 mm x 20 dishes

166-0500


4 AA batteries)

(Without Wood Lamp)
355-0717
1 chamber
(Wood Lamp)
355-0718
14 |
1 UV tube (366 nm)
BCYE Biplate
Pathogens Testing
ISO standards methods

NF EN ISO 11731-2 (07/2008):
Water quality
of Legionella
Part 2: Direct membrane-filtration method
for water with low bacterial counts
NF T90-461/A1 (04/2006):
Water quality
Legionella spp. and Legionella pneumophila
Method by direct inoculation
and after concentration by membrane filtration
or centrifugation
Water sample
Membrane filtration (1 L)
Ultrasonication or raising or centrifugation of the membrane
Concentrate
direct inoculation
0.1 ml
GVPC
heat treatment
0.1 ml
GVPC
acid treatments
0.2 ml
GVPC
8-10 days
36C
Confirmation
of Legionella spp.
or
BCYE with
L-Cysteine
BCYE without
L-Cysteine
(or Blood Agar

or Nutrient Agar)
48 hrs
36C
Confirmation of Legionella pneumophila

MonoFluo Kit or
Legionella pneumophila Latex kit
Note: Legionella Latex Kit Quick Guides are available on request
| 15
Catalog #
Legionella DNA extraction Kit - Bio-Rad solution
Description
Aquadien Kit
357-8121
96 tests
Extraction step
Aquadien DNA extraction Kit
NF Validation certified according
Water quality - Detection and quantification of
by concentration and genic amplification
No BRD 07/15 -05/11 ;
Aerosol sample
Also available:
Protocol for clogging samples
Biofilm sample
Water sample
Aquadien W2, Wash Solution

357-8119
48 tests
Aquadien Kit
Lysis of bacteria using thermal shock
95C
15 min
95C
Purification of DNA using Ultrafiltration

2 x10 min
Key Benefits
High extraction yield
Compatible with all type of water
(drinking water as well as cooling
tower)
NF Validation certified
Ready-to-use reagents
Simple protocol
16 |
Elution of DNA for real-time PCR analysis
Pathogens Testing
Legionella spp. and Legionella pneumophila
Alternative real-time PCR method
Catalog #
Analysis step
Description
Reagent kits
iQ-Check Quanti Legionella spp. Kit
iQ-Check Legionella real-time PCR Kits

to NF T90-417 (04/2010):
Legionella spp. and/or Legionella pneumophila
by polymerase chain reaction (PCR)
BRD 07/16 -05/11
357-8102
96 tests
iQ-Check Quanti L. pneumophila Kit

357-8103
96 tests
iQ-Check Screen Legionella spp. Kit
357-8104
96 tests
iQ-Check Screen L. pneumophila Kit

357-8105
96 tests
DNA Extraction Aquadien Kit

Instruments and software
Real-time PCR Cycler
New
359-3990

Industrial Diagnostic CFX 96

Real-Time PCR Detection
System, 96 wells
359-3893

CFX Manager Software

Industrial Diagnostic Edition
!
Software
Real-time PCR amplification

Documentation
Method Validation Guide
(NF T90-417 / 2010)
Screening
iQ-Check Screen
Legionella spp.
Quantification
iQ-Check Quanti
Legionella spp.
iQ-Check Screen
L. pneumophila
iQ-Check Quanti
L. pneumophila
Automatic result analysis and interpretation
Key Benefits
Just 5 hours to the result with no
confirmation
Detection of Legionella spp. and
L. pneumophila in the same assay
A 96-wells format adaptable to all
routine throughput
Ready-to-use reagents
Fully automated results analysis
Reference Material provided
Connected to the Standard
Reference Material
NF Validation certified

| 17
Catalog #
Legionella detection in aerosols

with CIP10-M Air Sampler - Bio-Rad solution
Description
Reagent
Aquadien Lysis Solution
CIP10-M
Method for the monitoring of airborne micro-organisms,
especially Legionella in aerosols
from cooling towers and droplets
Instruments
CIP10-M* Air Sampler/220V
359-3084

1 sampler and accessories

for 3 sampling
CIP10-M* Air Sampler/110V

359-3094

1 sampler and accessories

for 3 sampling
* CIP10-M is a trademark of ARELCO
Aerosol sampling
CIP10-M
Real-time NF T90-471
CIP10-M Air Sampler
Legionella DNA Extraction

Aquadien Kit
(See p. 16)
Key Benefits
Simulates human breath
Ergonomic
Portable
Free of cross contamination
Suitable for both culture media
(on-going studies) and real-time
PCR analysis
18 |
Standard Culture ISO 11731
Real-time PCR amplification

iQ-Check Legionella
Kits
Detection or quantification
(See p. 17)
Legionella 2009 poster

The hospital tap waters:
a risk of Legionella contaminated aerosol inhalation
M. Reyrolle, C. Landelle,
C. Mazure, MC. Nicolle,
S. Jarraud, J. Etienne, P. Vanhems
Pathogens Testing
Legionella detection in biofilms, amoeba Bio-Rad Technical Notes

Technical note #
Description
16249
Bio-Rads Legionella Testing Solution
French version available only
16186
Aerosols Testing
16995
Biofilms Testing
16225
Intra-amoebic Legionella
Legionella 2009 Poster
M. Reyrolle, C. Landelle, C. Mazure, MC. Nicolle,

S. Jarraud, J. Etienne, P. Vanhems
Method Validation Guide (T90-471 / 2010)
The hospital tap waters: a risk of

Legionella contaminated aerosol inhalation
| 19
Pseudomonas aeruginosa - EN ISO standards
NF EN ISO 16266 (08/2008)

Water quality
Pseudomonas aeruginosa
Membrane-filtration method
Membrane
CN Agar
48 hrs
37C
Reading/enumeration
Reading
UV lamp
Nutrient Agar (water testing)
24 hrs
37C
Acetamide Broth
24 hrs
37C
Nessler reagent
Reading/enumeration
ammonia producing
colonies
Oxidase test
Oxidase disks
King B
5 days
37C
Reading at = 366 nm
UV lamp
Enumeration
fluorescent colonies
20 |
Pathogens Testing
Pseudomonas aeruginosa
RAPIDP.aeruginosa alternative method
Catalog #
RAPIDP.aeruginosa
Chromogenic agar medium
for the direct detection and enumeration
of Pseudomonas aeruginosa by membrane-filtration
New
Description
Acetamide/Broth
355-4355
5 ml x 25 tubes
CN/Agar
356-3915
355-6034
356-4899
55 mm x 10 dishes
200 ml x 6 bottles
500 g
Equipment for Fluorescence

Detection
335-0717

335-0718
Portable UV observation
chamber (without wood lamp)
Wood lamp

166-0500


4 AA batteries)
King B/Agar
355-5278
Membrane
7 ml x 25 tubes
Nessler Reagent
22-30 hrs
36C
RAPIDP.aeruginosa
356-4194
100 ml x 1 vial
Nutrient/Agar (for Water Testing)

356-3786
90 mm x 20 dishes
Oxidase Disks
355-3832
box of 30 disks
!
New RAPID'P.aeruginosa/Agar
356-3984
356-4900
Enumeration of typical colonies:

Blue to blue-green colonies
55 mm x 20 dishes
500 g
Key Benefits
High specificity for
a direct enumeration
of Pseudomonas aeruginosa
Complete results within 22-30 hrs
Good colony coloration contrast
for a rapid reading
| 21
Catalog #
Salmonella spp. - ISO standard
Description
Brilliant Green Red Lactose

Phenol (or Kristensen or Edel and
Kampelmacher)/Agar
356-4464
500 g
Buffered Peptone Water/Broth

355-4179
356-4684
25 ml x 6 bottles
500 g
Kligler-Hajna/Agar
355-5378
356-4844
10 ml x 25 slanted tubes
500 g
LDC-ODC-ADH/Broth
355-3725
5 ml x 3 bottles
MKTTn (Muller-Kauffmann
Tetrathionate Novobiocin)/Broth
355-6140
356-4714
No filtration
(if volume < 10 ml)
10 ml x 50 tubes
500 g
Nutrient Agar (2.1%) without NaCl

356-4485
500 g
Physiological Sterile Water

(0.85%)
355-4164
9 ml x 25 tubes
RVS (Rappaport Vassiliadis Soya)/

Broth
355-5773
356-4324
10 ml x 25 tubes
500 g
TSI (Triple Sugar Iron)/Agar

356-4384
24-48 hrs
41.5C
First medium
XLD
500 g
XLD (Xylose Lysine Desoxycholate)/

Agar
356-9124
500 g
24 hrs
36C
(TSI,
22 |
Pathogens Testing
ISO 19250 (07/2010)

Water quality
Salmonella species
Water sample
Filtration
(if volume > 10 ml)
Membrane
Buffered Peptone Water
18 hrs
36C
RVS Broth or MKTTn

24 hrs
36C
Second medium left to choice

Kristensen
(Brilliant Green/Phenol Red Agar)
24 hrs
36C
Nutrient Agar (2.1%)

24 hrs
36C
Biochemical
LCD, Kliger-Hajna, urea agar)
and if appropriate
serological confirmation
| 23
Catalog #
Staphylococcus aureus NF standard
Description
Baird-Parker/Agar
Base Medium
356-4814
500 g
Baird-Parker/Agar
Complete Medium
356-3991
90 mm x 20 plates
Baird-Parker for RPF/Agar

Complete Medium
356-3996
357-8618

90 mm x 20 plates
90 ml x 6 bottles
+ 6 vials supplement
BHI (Brain-Heart Infusion)/Broth

355-3664
356-4014
10 ml x 25 tubes
500 g
Chapman Mannitol (for Water

Testing)/Agar
356-3926
356-4824
55 mm x 10 dishes
500 g
Chapman Mannitol (Water)
Additives
Egg yolk with potassium tellurite
355-4201
5 ml x 1 vial
355-4205
25 ml x 1 bottle
48 hrs
37C
Pastorex Staph Plus Kit
355-6353
355-6356
Enumeration of white
colonies with yellow
halo/Gram+
5 x 50 tests
50 tests
Rabbit Plasma Kit

355-6352
Pack for 20 reactions
RPF (Rabbit Plasma Fibrinogen)/

Supplement
356-4618
Transfer
the membrane
10 vials
Sulfamethazine Solution (0.2%)/

Supplement
356-2682
2.5 ml x 1 vial
Confirmation
Latex Pastorex Staph+
Confirmation
Brain Heart Infusion
48 hrs
37C
Coagulation reading:
Rabbit Plasma
24 |
Pathogens Testing
XP T90-412 (06/2006):
Water quality
of pathogenic Staphylococci
Membrane-filtration method
Membrane
or
or
Baird-Parker
Baird-Parker + RPF
48 hrs
37C
48 hrs
37C
Direct enumeration of black

or grey colonies surrounded
by a whitish opaque halo/Gram+
Enumeration of black
colonies with clear
halo/Gram+
Confirmation
Baird-Parker + RPF
48 hrs
37C
Enumeration of black
or grey colonies surrounded
by a whitish opaque halo/
Gram+
| 25
Micro-organisms generally detected and methods the most commonly

Treated Water
Main types
of water
Network Tap
Industrial
Process
Artificial Surface
Bottled
Origins of water
Drinking water
Treated cooling
towers, process,
sanitary water
Spa, hydrotherapy, swimming

pool, whirlpools
Natural mineral
and source water
Turbidity
Microbial load
MF (EUR) P/A (US)
MF
MF
MF
Typical quality
indicators
Total flora
E. coli
Coliforms
Enterococci
Total flora
E. coli
Coliforms
Enterococci
Total flora
E. coli
Coliforms
Enterococci
Total flora
Coliforms
(E. coli)
Typical
pathogens
Legionella
P.aeruginosa
Salmonella
SRA
Legionella
P.aeruginosa
Salmonella
SRA
Legionella
P.aeruginosa
Staph. aureus
Salmonella
SRA
Typical methods
Legends:
SRA = Sulfito-Reducing Anaerobes
MF = Membrane filtration
MPN = Most Probable Number
P/A = Presence/Absence
PCR = Polymerase Chain Reaction
26 |
Legionella
P.aeruginosa
Synthesis
used according to the origin of water sample

Non-Treated Water
Superficial
(Surface)
Bathing
Waste
Cooling Towers
Aerosols
Lakes, rivers
Coastal sea,
estuarine, inland
water
Residual raw
waters, sludge,
soil, treatment
plant effluents
Non treated
cooling towers
Drops from
cooling towers,
tap water
+++
+++
+++
++++
++
++
++
+++
+++
+++
MF/MPN
MF/MPN
Impedance
MPN/PCR
New ! Impedance
MPN/PCR
PCR
Total flora
E. coli
Coliforms
Enterococci
Total flora
E. coli
Coliforms
Enterococci
Total flora
E. coli
Coliforms
Enterococci
Total flora
Total flora
Salmonella
SRA
Legionella
Salmonella
Legionella
Legionella
| 27
Bio-Rad, S.N.C. au capital de 50 000 000 Euros, Locataire-Grant, 449 990712 RCS Nanterre N Intracommunautaire FR 62 449 990 712 Siret 449 990 712 00019
Life Science
Group
Website www.bio-rad.com/diagnostics U.S. 1-800-2BIO-RAD Australia 61-2-9914-2800 Austria 43-1-877-8901 Belgium 32-9-385-5511 Brazil 5521-3237-9400
Canada 1-514-334-4372 China 86-21-64260808 Czech Republic 420-241-430-532 Denmark +45-4452-1000 Finland 358-9-804-22-00 France 33-1-47-95-60-00
Germany +49-(0)89-318-840 Greece 30-210-7774396 Hong Kong 852-2789-3300 Hungary +36-1-459-6100 India 1-800-180-1224 Israel 972-3-9636050 Italy +3902-216091 Japan 81-3-6361-7070 Korea 82-2-3473-4460 Mexico +52 (55) 5488-7670 The Netherlands +31-318-540666 New Zealand 64-9-415-2280 Norway
47-23-38-41-30 Poland 48-22-3319999 Portugal 351-21-472-7700 Russia 7-495-721-14-04 Singapore 65-6415-3188 South Africa 27-11-442-85-08 Spain 34-91590-5200 Sweden 46-8-555-127-00 Switzerland 41-61-717-95-55 Thailand 662-651-8311 United Kingdom +44-(0)20-8328-2000
Printed in France with soy-based inks on paper from sustainably managed forests
Bio-Rad 25 17932 Rev F 07/2011

Bio Rad Water

Uploaded by

Document Information

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Bio Rad Water

Uploaded by

Copyright:

Available Formats

Bio-Rad Laboratories Water Testing

Guide for Bio-Rad Products in Water Testing

Guide for Bio-Rad Products in Water Testing

E. coli EN ISO miniaturized MPN standard

E. coli Automated alternative method: XplOrer64 System

E. coli/Coliforms NF EN ISO standards

E. coli/Coliforms RAPIDE.coli 2 alternative method

Enterococci EN ISO miniaturized MPN standard

Enterococci Automated alternative method: XplOrer64 System

Enterococci EN ISO standard

Enterococci RAPIDEnterococcus alternative method

Part 2 Pathogens Testing

Legionella spp. & Legionella pneumophila NF EN ISO standards methods

Legionella detection in aerosols with CIP10-M Apparatus Bio-Rad solution

Legionella detection in biofilms, Amoeba Bio-Rad technical notes

Pseudomonas aeruginosa EN ISO standards

Salmonella spp. ISO standard

Staphylococcus aureus NF standard

Quality Indicators Testing

PCA (Plate Count Agar)

PCA without Dextrose/Agar

Guide for Bio-Rad Products in Water Testing |

E. coli - EN ISO miniaturized MPN standard

DSM (Dehydrated Special

EDS (Distilled Water for Microplates)

EN ISO 9308-3 (03/1999)

Data management software for the statistical

MUG/EC E. coli Microplates Kit

25 opaque white plates

Long-Wave UV Lamp, Compact Portable

1 lamp (366 nm, requires

Portable UV Observation Chamber

Square Petri dishes

120 mm x 252 units

1 UV tube (366 nm)

| Guide for Bio-Rad Products in Water Testing

Calculation and interpretation

Quality Indicators Testing

E. coli - Automated alternative method:

Automated real-time and continuous quantification

Samples with high

Non filterable water

Bathing water (sea, inland)

CheckNSafe E.coli Kit

CheckNSafe Screw Caps

Sterile caps (1 bag of 30 units)

Automated detection or quantification with

- bathing water: 8 hrs

Automated real-time results analysis

First alternative method certified

Guide for Bio-Rad Products in Water Testing |

BCP (Bromocresol Purple)

E. coli/Coliforms - NF EN ISO standards

BGBLB (Brilliant Green Bile

Water sample filtration

Water sample (dilutions)

BCP Lactose Broth

Peptone Water (without Indole)/Broth

Trouble and gas+

TTC Tergitol 7/Agar - Base Medium

TTC Tergitol 7/Agar - Complete Medium

Tryptose Lauryl Sulfate/Broth

(10) yellow colonies

TSA Tryptophane Membrane on