Professional Documents
Culture Documents
Macerated plant materials are excellent for the study of plant cell types. Firstly I
prepared the macerating fluid as follows:
30% solution of hydrogen peroxide
4 part water
Part glacial acetic acid
Procedure
1. I cut the plant tissue of stem into small pieces of not more than 1 mm
thick
2. I put the tissue into freshly prepared macerating fluid. The fluid is
prepared by mixing solution of hydrogen peroxide and acetic acid
3. I Left the tissue in the macerating fluid for about seven days.
4. I teased the tissue with dissecting needles. The cells did not separate
readily; I left the tissue in the macerating fluid for some more days.
5. I filtered off the macerating fluid and washed away the acids from the
macerated material with water.
6. Then I stored macerated plant material in 70% ethanol.
7. Then I mounted the macerated material to make a temporary slide of it.
Observation
1. I saw trichomes ( hair like structure to prevent the plant from pathogens)
2. spirals like structure which was actually primary thickening meristem
3. parenchyma cell
4. fibers
5. vessel elements
Slide
I saw the slide and identifed that the type of stomata was paracytic
Observation
1. Type of venation
Result
The type of venation for this plant is reticulate