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Lesi yang berdekatan pada pasien laki-laki atau perempuan biasanya bergabung dan membentuk lesi konfluens.
viability of H. ducreyi for more than 4 days at 4 C. More reliable results are obtained if the
exudate from the ulcer is inoculated directly onto the plate, not onto
transport medium. Plates are incubated at 33 C in microaerophilic conditions and
examined for growth in 48 hours.
H. ducreyi cannot be cultured on routine medium. Nutritional requirements of H. ducreyi
seem to be geographically defined. High cultural yield is obtained by using
Mueller-Hinton agar base supplemented with chocolate horse blood and
Isovitale X (MH-HBC).