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Paper # 183/184
Chemistry/ Microbiology
2.277/60.277
Instructions
Please mark the Answer Sheet using PENCIL ONLY.
Enter your NAME and STUDENT NUMBER on the Answer Sheet.
The exam consists of multiple choice questions. Enter your answers on the Answer Sheet.
There is only 1 correct answer for each question.
______________________________________________________________________________
1.
Hydrophobic interactions:
A) refer to the ability of water to denature proteins.
B) do not contribute to the structure of water-soluble proteins.
C) are the driving force in the formation of micelles of amphipathic compounds in
water.
D) are explainable in terms of solute enthalpy.
E) primarily involve the effect of polar solutes on the entropy of aqueous systems.
2.
3.
i and ii.
ii and iv.
iii, iv, and v.
ii, iii, and iv.
i and v.
7.4
7.22
7.58
6.53
6.8
4.
Lemon juice at pH 2.3 contains about ___ times as much H+ as milk of magnesia at pH 10.5.
A) 8.2
B) 1.6x108
C) 10-8
D) 12
E) 100,000
5.
75 mL of 0.1M HCl were added to 100 mL of 0.1M aspartate solution, pH=pI. What is
the new pH? pKa values for aspartate are 1.88, 3.65 (R-group) and 9.60.
A) 2.76
B) 4.13
C) 6.62
D) 1.40
E) 9.60
6.
For any amino acid with a non-polar R-group, at any pH below the pI of the amino acid,
the predominant form in solution will:
A) have a net positive charge.
B) have no net charge.
C) have a net negative charge.
D) have both positive and negative charges in equal concentration.
E) be a Zwitterion.
7.
8.
HOOC
COOH
A)
B)
C)
D)
E)
9.
H
COOH
In the diagram below, the plane drawn behind the peptide bond indicates the:
A)
B)
C)
D)
Which of the following best represents the backbone arrangement of two peptide bonds?
A) CNCCCNCC
B) CCNCCN
C) CNCCNC
D) CNCCCN
E) CCCNCCC
11.
12.
13.
14.
Which of the following statements about a plot of V0 vs. [S] for an enzyme that follows
Michaelis-Menten kinetics is false?
A) As [S] increases, the initial velocity of reaction, V0, also increases.
B) The shape of the curve is a hyperbola.
C) At very high [S], the velocity curve becomes a horizontal line that intersects the
y-axis at Km.
D) Km is the [S] at which V0 = 12 Vmax.
E) The y-axis is a rate term with units of moles per litre/min.
15.
An enzyme-catalyzed reaction was carried out with the substrate concentration initially 10
times greater than the Km for that substrate. After 15 minutes, 2% of the substrate had
been converted to product, and the amount of product formed in the reaction mixture was
45 mol. If, in a separate experiment, one-third as much enzyme and twice as much
substrate had been combined, how long would it take for the same amount (45 mol) of
product to be formed?
A)
B)
C)
D)
E)
16.
45 min
90 min
27 min
15 min
5 min
Which of the following definitions correctly identifies the common name of the
compound?
A) Maltose = Glc (14) Glc
B) Lactose = Gal (14) Fru
C) Sucrose = Fru (12) Fru
D) Cellulose = (Glc 14 Glc)n
E) Iso-maltose = Glc (16) Glc
17.
18.
In the following compound carbon ____ (i) is the anomeric C, and carbons _____ (ii) are
asymmetric.
H
4C
OH
A)
B)
C)
D)
E)
19.
6CH OH
2
5C
O
H
OH
3
C
H
2
C
OH
1
C
OH
H
i = 6 and ii = 2, 3, 4, and 5
i = 2 and ii = 3, 4, 5, and 6
i = 1 and ii = 1, 2, 3, 4, and 5
i = 5 and ii = 6
i = 1 and ii = 1, 2, 3, 4, 5, and 6
HO C
H C
OH
H C
OH
CH2OH
A)
B)
C)
D)
E)
20.
Glyceraldehyde
D-Ribose
D-Fructose
L-Glucose
Vitamin C
2&4
3, 4 & 5
3&4
1, 2 & 3
1&2
-O
A)
B)
C)
D)
E)
22.
H2
C
C
C
H2
C
H2
H2
C
C
H2
H2
C
H2
C
C
H2
H2
C
C
H2
H2
C
C
H2
H2
C
H2
C
C
H2
C
H2
C H3
2, 3, 4 and 5
1 & 5 only
4 and 5 only
2 and 3.
1, 2 & 3
23.
24.
25.
HO
A)
B)
C)
D)
E)
Cholesterol
Dolichol
Ubiquinone
Phospholipase C
Testosterone
Arachidonate
Phosphatidyl serine
Palmitate
Cholesterol
Sphingosine
27.
28.
29.
The proportion of bases that are purines must be the same in both strands.
The planes of the bases lie perpendicular to the long axis of the DNA molecule.
The 2' hydroxyl groups of ribose participate in hydrogen bonding.
The two strands are antiparallel.
The adenine content of one strand must be equivalent to the cytosine content in the
complementary strand.
A)
B)
C)
D)
E)
30.
2 & 4.
1,3, & 4.
2,4, & 5.
1 & 5.
3 & 5.
The polymer (5') ATTCGCAGTAA (3') could form a double-stranded structure with:
A)
B)
C)
D)
E)
32.
33.
P O
P O
P O CH2
N
N
H
OH OH
A)
B)
C)
D)
E)
34.
indicate formation of P is slow and equilibrium has not yet been reached.
indicate equilibrium has been reached.
indicate an enzyme has shifted the equilibrium toward S.
are impossible, since G0' is -70 kJ/mol, so there must have been an error in
the analysis.
E) indicate formation of P is thermodynamically unfavourable.
35.
The standard reduction potentials (E0') for the following half reactions are given.
Oxaloacetate/malate
E0' = -0.18 V
+
+
NAD /NADH + H
E0' = -0.32 V
If you mixed oxaloacetate, malate, NAD+ and NADH + H+ together, all at 1 M
concentrations and in the presence of malate dehydrogenase, which of the following
would happen initially?
A) Malate would be oxidized, NAD+ would be reduced.
B) No reaction would occur, because all reactants and products are already at
their standard concentrations.
C) Oxaloacetate would be reduced, NADH + H+ would be oxidized.
D) Oxaloacetate and malate would be oxidized; NAD+ and NADH + H+would be
reduced.
E) Malate would be oxidized, NADH + H+would be unchanged.
37.
9.12 x 10-4
1.76 x 10-16
21.0
4.8 x 10-2
0
The standard reduction potentials (E0') for the following half reactions are given.
acetaldehyde/ethanol
NAD+/NADH + H+
-0.20 V
-0.32V
Calculate the equilibrium constant for the reaction in which ethanol is formed via
alcoholic fermentation in yeast. R = 8.3 J/mol.K, T = 298 K, F (Faraday constant) = 96.4
kJ/volt*mol The value is ?
A)
B)
C)
D)
E)
38.
108
1.15 X 104
8.7 X 10-5
9.26 X 10-3
1.12
The conversion of one mole of glucose to two moles of lactate by the glycolytic pathway
results in the net formation of:
A) Four moles of ATP.
B) Two moles of NADH and two moles of ATP.
C) Two moles of NADH and one mole of ATP.
D) Two moles of NAD+ and two moles of ATP.
E) Two moles of ATP.
39.
The first reaction in glycolysis that results in the formation of an energy-rich compound (i.e.,
a compound whose hydrolysis has a highly negative G0') is catalyzed by:
A)
B)
C)
D)
E)
40.
phosphoglycerate kinase
hexokinase
glyceraldehyde-3-phosphate dehydrogenase
phosphofructokinase
triose phosphate isomerase
Reactions of glycolysis and the TCA cycle are regulated by compounds that signal the
energy status of the cell: Identify the correct statement.
A)
B)
C)
D)
E)
Which of the following steps of the TCA cycle is/are linked to the reduction of FAD?
1) succinate fumarate
2) citrate isocitrate
3) malate oxaloacetate
4) fumarate malate
5) succinyl-S-CoA succinate
A)
B)
C)
D)
E)
43.
Which of the following co-enzymes contains a nucleotide base also found in DNA and
RNA?
1. Thiamine Pyrophosphate
2. Coenzyme A
3. Lipoate
4. FAD
5. NAD
A.
B.
C.
D.
E.
44.
4
5
2&5
1
1&3
1 and 3
2, 4, and 5
3, 4 and 5
1, 2, 3, 4, and 5
1, 2, 4, and 5
45.
46. Which of the five amino acids listed below is the unknown amino acid?
pKa (- COOH)
A)
B)
C)
D)
E)
Arginine
Aspartic acid
Phenylalanine
Serine
Alanine
pKa (- NH3+)
2.2
2.4
1.8
2.2
2.4
9.0
9.8
9.1
9.1
9.8
pKa (R-gp)
12.5
4.0
----
B) 3.2
C) 4.0
D) 6.1
48. To 100 mL of a 0.1 M solution at pH 9.8 of the same unknown amino acid from questions 46
and 47 was added 25 mL of 0.2 M HCl. What is the new pH of the solution?
A) 3.2
E) 6.9
B) 4.0
C) 5.4
D) 6.1
B) 1.16 x 10-3M
E) 2.05 x 10-5M
C) 5.50 x 10-5M
50. What was the molar concentration of the oxidised form of the electron carrier in the original
sample?
A) 4.58 x 10-3M
D) 0.58 x 10-5M
B) 1.16 x 10-3M
E) 0.00M
C) 2.29 x 10-5M
51. Which of the following enzymes could be assayed using this technique?
1.
2.
3.
4.
5.
Glyceraldehyde-3-phosphate dehydrogenase
Lactate dehydrogenase
Pyruvate dehydrogenase
Isocitrate dehydrogenase
Succinate dehydrogenase
A) 1 and 2
E) All of the above
B) 3, 4 and 5
C) 1, 3, 4 and 5
D) 1, 2, 3 and 4
52. In your experience in the biochemistry labs which of the following statements about paper
chromatography are true?
1. Separation of sample components is based upon their partition between two immiscible liquid
phases.
2. Water forms the stationary phase while a less polar solvent forms the moving phase.
3. The less polar a component the less it moves.
4. The more polar a component the more it moves.
5. The Rf is calculated by taking the ratio of the distance moved by a sample component over
distance moved by solvent.
A) 1 and 2
E) None of the above
B) 1, 2 and 5
C) 1, 3 and 4
53. From the graph determine Vmax in moles product formed/min, for the reaction under the
conditions cited.
A) 0.232
D) 3.720
B) 0.456
E) Cannot be determined
C) 2.500
54. Which of the following modifications of the experiment or the data would enable you to
determine Vmax more accurately?
1.
2.
3.
4.
A) 2 and 3
B) 1, 2 and 3
C) 4
D) 3 and 4
55. Which of the following pairs refers to substrate and product listed in that order for the enzyme
alkaline phosphatase?
A) 1 and 2
E) 3 and 1
B) 1 and 3
C) 2 and 3
D) 3 and 2
56. Which of the reagents brings about color development upon termination of the alkaline
phosphatase assay?
A) 1
B) 2
C) 3
D) 4
E) 5
57. What information can be determined from a Lineweaver-Burk plot of enzymatic data giving
the following values 1/v is 0(zero) when 1/[S] is -2.5 x 102M?
A)
B)
C)
D)
E)
58. The enzyme assay performed in the lab this term relied upon which of the following
conditions?
1.
2.
3.
4.
5.
A)
E)
C) 1, 2 and 4
D) 1, 2, 3 and 4
B) 1, 2, 3 and 4
C) 2 and 3 and 4
D) 2 and 3
60. Which of the following statements describe the role of sodium citrate in the isolation of DNA
from salmon nuclei?
A)
B)
C)
D)
E)
A) 3
E) 3 and 4
B) 4
C) 5
D) 1 and 2