Professional Documents
Culture Documents
UV-VIS-NIR Spectrophotometer
UV-3600
UV-VIS-NIR Spectrophotometer
UV-3600
High Sensitivity
We developed the first UV-VIS-NIR spectrophotometer in the world with three detectors. These consist of a PMT
(photomultiplier tube) for the ultraviolet and visible regions and an InGaAs detector and a cooled PbS detector for
the near-infrared region. With conventional instruments, there is a drop in sensitivity in the crossover between the
regions covered respectively by the PMT and the PbS detector. Using an InGaAs detector to cover this region,
however, ensures high sensitivity across the entire measured wavelength range. The 1,500-nm noise level does not
rise above 0.00003 Abs, which is the lowest level in the world.
High Sensitivity
The UV-3600 can handle measurement with highly precise transmittance and reflectance, and uses three detectors to
handle a range going from the ultraviolet region to the near-infrared region. The level of sensitivity for the
near-infrared region has been increased significantly by using an InGaAs detector and a cooled PbS detector for this
region. Spectra can be obtained for the entire range, from the ultraviolet region to the near-infrared region, with a
high level of sensitivity and precision.
InGaAs
InGaAs detector
PbS
PbS detector
PMT
Optical system around detectors
Sensitivity Characteristic
100
PMT detector
PMT
PbS
InGaAs
1000
2000
3000
Wavelength (nm)
380 nm
UV
PMT
InGaAs
PbS
780 nm
Visible
3300 nm
NIR
165~1000 nm
700~1800 nm
1600~3300 nm
Switching between the photomultiplier tube and the InGaAs detector is possible in the range 700 to 1,000 nm (the default switchover wavelength is 830 nm).
Switching between the InGaAs detector and the PbS detector is possible in the range 1,600 to 1,800 nm (the default switchover wavelength is 1,650 nm).
0.300
T%
0.200
InGaAs detector
0.100
PbS detector
0.000
-0.050
1370.0
1400.0
1450.0
1500.0
1550.0
1600.0
nm
0.400
1.500
0.300
Abs.
Abs.
1.00
0.200
0.500
0.100
0.000
700.0
800.0
900.0
nm
1000.0
0.000
1550.0
1600.0
1650.0
1700.0
nm
The figures above on the left and right are, respectively, transmittance spectra for ethylbenzene (obtained using a cell with an optical path of 100 mm) and cyclohexane
(obtained using a cell with an optical path of 10 mm). There is hardly any level difference at the respective detector changeover wavelengths (870 and 1,650 nm).
UV-3600
UV-VIS-NIR Spectrophotometer
0.350
0.300
1.000
Abs.
Abs.
1.500
0.250
0.200
0.500
0.150
0.000
240.0
245.0
250.0
nm
255.0
0.100
249.0
260.0
249.5
250.0
nm
250.5
251.0
The spectrum shown on the left was obtained by enclosing benzene gas in a cell with an optical-path length of 10 mm and performing measurement. The spectral
bandwidth is 0.1 nm. The triplet in the neighborhood of 250 nm (enlarged above) can be observed clearly. This instrument allows high-resolution spectra to be measured
with little noise.
100.000
800.00
0.00005
T%
T%
60.00
40.00
0.00000
20.00
0.100
330.0 340.0
350.0
360.0
nm
370.0
-0.00005
330.0
380.0 390.0
335.0
340.0
nm
345.0
350.0
The figure above on the left is a spectrum for aqueous NaNO2 solution, and the figure on the right shows an enlarged view of the neighborhood of 340 nm. In the
figure on the right, the red spectrum is for aqueous NaNO2 solution and the blue spectrum is the 0% line obtained when a shutter block is inserted on the
sample-beam side. The UV-3600 achieves an ultra-low stray-light level of less than 0.00005% at 340 nm.
(A mesh filter is used on the reference-beam side to maintain balance with the sample-beam side.)
6.000
4.000
Abs.
Abs.
4.000
2.000
2.000
0.000
250.0 300.0
Calibration curve
400.0
500.0
nm
600.0
700.0
0.000
0.000 100.000 200.000 300.000 400.000
Concentration (mg/L)
The UV-3600 is equipped with a high-performance, grating-grating double monochromator, and achieves a low
stray-light level with high resolution. The wavelength range is 185 to 3,300 nm. This instrument can perform
spectrometry for various types of sample, ranging from samples requiring a high resolution, such as gas, to highconcentration liquid samples.
100.000
80.000
T%
60.000
40.000
20.000
0.000
185.0
1000.0
2000.0
3000.0 3300.0
nm
The figure on the left shows the spectrum for a low-transmittance film on a
silica wafer in the range 200 to 1,600 nm. Although the film is a special type of
film with a transmittance of almost zero, it has been measured with high
precision and little noise. (A mesh filter is used on the reference-beam side to
maintain balance with the sample-beam side.)
0.150
T%
0.100
0.050
0.000
-0.010
200.0
500.0
1000.0
1500.0 1600.0
nm
2.500
20C
40C
60C
2.000
Abs.
1.500
1.000
Isolate-OH
0.500
Aggregate-O
OH
0.000
1300.0
1400.0
1500.0
1600.0
1700.0
1800.0
nm
UV-3600
UV-VIS-NIR Spectrophotometer
UVProbe Software
All-In-One Software
UVProbe is an all-in-one software package equipped with
the following four functions:
Spectrum module
Photometric module (quantitation)
Kinetics module (time-course measurement)
Report generator
Each can be easily operated from its own special screen. In
addition to a wealth of data processing functions, including
peak detection and area calculations, the software is
equipped with security functions to configure operational
authority user by user, as well as data audit trail and
equipment audit trail functionality.
Photometric Module
Spectrum Module
Kinetics Module
Report Generator
The report generator provides the freedom to arrange graphs, tables, etc. to suit
users' needs. The thickness and color of graph lines, as well as font size, can now
be specified. Pasting labels on graphs and editing text is as easy as can be,
allowing the user to effectively print comments along with the analysis results.
GLP/GMP Support
Security Functions
The use of functions can be restricted according to
the user level.
Audit Trail Function
Details of processes (e.g., baseline correction) that
affect measurement data are tracked in the
instrument's history.
Data History Function
If a change is made to measurement data, a history
of this is added to the data.
DNA (single-stranded)
Bradford method
BCA Method
DNA (double-stranded)
et method
Lowry method
UV-3600
UV-VIS-NIR Spectrophotometer
Optional Software
UVProbe Agent Software
(P/N 206-21550-91)
The UVProbe Agent, which was developed for Shimadzu UV-VIS spectrophotometers, is used to automatically transfer and store the data acquired,
or the results of performing data processing with the UVProbe software,
to a general-purpose database, and to perform operations related to
high-security data management and electronic signatures. This makes it
possible for UVProbe to attain compliance with FDA 21 CFR Part 11.
The UVProbe Agent is network-compatible; therefore, by installing other
Agent software for corresponding analytical instruments, such as HPLC or
FTIR spectrophotometers, data from all analytical instruments can be
integrally managed at a server PC and browsed at client PCs.
Tm Analysis Software
(P/N 206-57476-91)
This software works with the S-1700 and accumulates temperatureversus-absorbance curve data at the PC to analyze the Tm (melting
temperature) of nucleic acids such as DNA and RNA.
Applicable OS: Windows 7 Professional/Vista Business
Note: An RS-232C cable (P/N 200-86408) is needed to connect the PC to the S-1700.
10
The software is fully equipped with convenient graphic functions including chromaticity diagrams and enlarged color-difference views.
It provides a wealth of recalculation functions, enabling items and conditions with
respect to the spectra obtained to be changed for recalculation.
The visual field (2, 10) and the illumination (A, B, C, D65, F6, F8, and F10) are
freely selectable. In addition, the user can configure particular weighting coefficients,
enabling calculations with respect to any illumination. The configured illumination
can also be saved.
Standard white plate values can be configured, enabling corrected calculations.
Measurement window
Recalculation window
UV-3600
UV-VIS-NIR Spectrophotometer
11
Accessories
Cells
Description
Type
20 mm
5.08.0 mL
50 mm
12.520.0 mL
100 mm
25.040.0 mL
10 mm
2.54.0 mL
Semi-micro cell
10 mm
1.01.6 mL
10 mm
1.01.6 mL
5 mm
25100 L
*2
10 mm
50200 L
10 mm
50400 L
10 mm
3.8 mL
Square cell
Cylindrical cell
20 mm
7.6 mL
50 mm
19.0 mL
100 mm
38.0 mL
1 mm
0.30.4 mL
2 mm
0.50.8 mL
5 mm
1.32.0 mL
2.54.0 mL
Glass (G)
200-34442
200-34565
200-34446
200-34446-01
200-34944
200-34944-01
200-34676
200-34676-01
200-34444
200-34444-01
*1
200-66501
200-66501-01
*1
200-66551
208-92116
*2
200-66578-11
*2
200-66578-12
200-34472-01 (
200-34473-01 (
200-34473-03 (
200-34473-02 (
200-34473-04 (
200-34472 (
200-34660-01
200-34662-01
200-34655
200-34662-11
200-34449
200-34449-01
4
12.5
25
45
12
12
12
12
.5
12.5
.5
2
2
Unit: mm
Note *1 With a 5 mm slit, the cell holder with micro cell mask (P/N 204-06896) is required.
*2 The supermicro cell holder (P/N 206-14334) is required.
( P/ N 204- 21 4 7 3 - * * )
204-21473-03
1 mm
204-21473-01
2 mm
204-21473-02
5 mm
12
Long-Path Rectangular
Cell Holder (P/N 204-23118-01)
(P/N 204-06216-02)
(P/N 206-14334)
(P/N 204-06896)
Required when using semi-micro cells or micro cells with an optical path width of 3 mm
or less.
(The mask width can be adjusted.)
UV-3600
UV-VIS-NIR Spectrophotometer
13
Accessories
8/16-Series Micro Multi-Cell
C ell Holde r s
Model
P/N
206-23680-91
206-23690-91
This cell holder holds one micro multi-cell, either 8 or 16 cell, for
micro-volume measurement. Two types of micro multi-cell holders are
available: the standard type (MMC-1600) and the constant-temperature
water circulation type (MMC-1600C).
Applicable temperature range: 10 C to 60 C (C type)
Temperature deviation between circular water and cell: max. 3 C (C type)
Temperature stabilizing time: 15min or less (C type)
P/N
8-series Micro Multi-cell; optical path length: 10 mm; cell volume: 100 L
208-92089
16-series Micro Multi-cell; optical path length: 10 mm; cell volume: 100 L
208-92088
208-92086
208-92085
There are two types of micro multi-cells available in both the 8-series and
16-series models: a 50L type and a 100L type. The cell intervals of the
8-series micro multi-cells are applicable for use with 8 12-well microplates
and 8-channel pipettes. Microplate samples aspirated into multi-channel
pipettes can be injected directly into the cells for measurement.
(P/N 206-23760-**)
14
(P/N 206-23780-**)
(P/N 202-30858-04)
(P/N 204-27206-02)
(P/N 206-23900-**)
110-QS-10
10 mm
3.5 mL
115B-QS-10
10 mm
400 L
(P/N 208-97263)
UV-3600
UV-VIS-NIR Spectrophotometer
15
Accessories
Sipper Unit 160L (Standard Sipper)
(P/N 206-23790-91)
(P/N 206-23790-92)
(P/N 206-23790-93)
(P/N 206-23790-94)
Four types of sipper units with different flow cell types are
available. The stepping motor-driven peristaltic pump ensures
reliable and smooth aspiration of sample solution.
(Direct driving is possible from the UV-3600 so no interface is required.)
Standard Sample Volume
Note: The use of a Solenoid Valve (Fluoropolymer) (P/N 204-06599-01) and
the SWA-2 Sample Waste Unit (P/N 206-23820-91) are recommended
when strong acids, strong alkalis, or organic solvents are to be measured.
1.5 mL
Syringe Sipper
Model
P/N
206-23890-91
206-23890-92
Note: Flow cell available separately. Choose from the recommended flow cells listed below.
Recommended Flow Cells
Cell Type
P/N
10 mm
2 mm
0.9 mL
Square (micro)
208-92113
10 mm
3 mm
1.0 mL
10 mm
5.0 mL
(P/N 206-23810-**)
16
2.5 mL
0.5 mL
(P/N 204-06222)
(P/N 204-27588-03)
(P/N 204-06222-01)
P/N
204-06222
10 mm
Volume
0.3 mL
204-06222-01
5 mm
0.15 mL
(P/N 206-12852)
Film Holder
(P/N 204-58909)
Sample Size
Minimum: 16 (W) 32 (H) mm
Maximum: 80 (W) 40 (H) 20 (t) mm
(P/N 206-89065-41)
(P/N 206-28500-41)
UV-3600
UV-VIS-NIR Spectrophotometer
17
Accessories
ISR-240A Integrating Sphere Attachment, 60 mm dia.
(P/N 206-23860-91)
Reference cell
for transmission
measurement
Sample
Sample beam
Optical diagram
(P/N 206-23851-91)
Reference cell
for transmission
measurement
Reference beam
Sample cell
for transmission
measurement
Sample beam
(P/N 206-23862-92)
(P/N 206-23862-91)
Reference
beam
Sample
beam
PM
PbS
Wavelength range:
LISR-3100 240 to 2400 nm; LISR-2100 240 to 800 nm
Integrating sphere: LISR-3100 150 mm in inner diameter, equipped
with a photo-multiplier and a PbS cell; LISR-2100 150 mm in inner
diameter, equipped with a photo-multiplier.
Space to mount reflection sample: About 150W 170H 30T mm
Incident angle: 7 deg.
(LISR-3100 only)
(P/N 206-23831-91)
660
UV-3600
V
MPC-3100
420
1020
Unit : mm
18
(P/N 206-14046)
Sample
(measuring
surface down)
Optics
Light beam
Structure
P/N
206-16817
206-16100
206-15001
206-15002
These accessories are intended for use with the MPC-3100 Sample
Compartment, and require the BIS-3100 Sample Base Plate-Integrating Sphere
Set (P/N 206-17059). At larger angles of incidence (30, 45), a polarizer is also
required.
Measurement wavelength range: 300 to 800 nm
Accuracy: with respect to 90 % reflectance samples
Incidence angle 5: 1.5 %
Incidence angle 12: 1.0 %
Incidence angle 30, 45: 2.5 %
100 % level sample setting: The sample measurement optical path can be switched using
the single-touch V-N method.
Approximate sample size: 25 to 200 mm dia., or 20 to 150 mm square, up to 30 mm thick
Note: The BIS-3100 Sample Base Plate-Integrating Sphere Set (P/N 206-17059) is required for
mounting these absolute specular reflectance attachments.
Ref
100 % light
M2
Measurement
light
Polarizer
position
M2'
M3
M1
Integrating
sphere
Sample
Construction
Effective Diameter
Wavelength Range
206-15694
20mm
250 to 2500nm
Polarizer Type I
206-13236-01
18mm
400 to 800nm
Polarizer Type II
206-13236-02
17mm
260 to 700nm
206-13163
11mm
260 to 2500nm
UV-3600
UV-VIS-NIR Spectrophotometer
19
Specifications
Hardware
Wavelength range
Spectral bandwidth
185 to 3,300 nm
8 steps in ultraviolet and visible regions: 0.1, 0.2, 0.5, 1, 2, 3, 5, 8 nm
10 steps in near-infrared region: 0.2, 0.5, 1, 2, 3, 5, 8, 12, 20, 32 nm
Resolution
0.1 nm
0.01 to 5 nm
Wavelength accuracy
The light source is switched automatically in accordance with the wavelength. The wavelength at which the light source
is switched can be set freely in the range 282 to 393 nm (in 0.1 nm increments).
Stray light
Photometric system
Double beam
Photometric range
6 to 6 Abs
Photometric accuracy
0.003 Abs (1 Abs), 0.002 Abs (0.5 Abs), determined with NIST930D standard filter
0.0008 Abs (0 to 0.5 Abs), 0.0016 Abs (0.5 to 1 Abs), determined using 1-second accumulation, taking the maximum
deviation over five measurements
Noise
0.00005 Abs max. (500 nm), 0.00008 Abs max. (900 nm), 0.00003 Abs max. (1,500 nm), determined using RMS values for
1-second responses, 2 nm slit width
Baseline flatness
0.004 Abs (185 to 200 nm), 0.001 Abs (200 to 3,000 nm), 0.005 Abs (3,000 to 3,300 nm)
Baseline stability
Within 0.0002 Abs/h (2 hours after power ON, 500 nm, 1-second accumulation)
Light sources
50W halogen lamp (2,000-hour service life), deuterium lamp (socket type, 2,000-hour service life),
built-in mechanism for automatically adjusting the light-source position
Monochromator
Detector
Sample compartment
Size
Weight
96 kg
Operating temperature
15C to 35C
Operating humidity
Power supply
100/120/220/230/240 VAC,
V
50/60 Hz
Power consumption
300 VA Note)
20
Sof tware
Data Acquisition Modes
General
Spectrum Mode
Data Processing in
Spectrum Mode
Photometric
(Quantitation) Mode
Report Generator
660
1020
275
Dim e n si o n s
Unit: mm
UV-3600
UV-VIS-NIR Spectrophotometer
21
UV-3600
Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its
affiliates, whether or not they are used with trademark symbol TM or .
Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu
disclaims any proprietary interest in trademarks and trade names other than its own.
For Research Use Only. Not for use in diagnostic procedures.
The contents of this publication are provided to you as is without warranty of any kind, and are subject to change without notice. Shimadzu
does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.
www.shimadzu.com/an/