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Lesson 8
Staphylococci and Diseases
Staphylococcus
Staphylococcaceae contains 4 genera, the
most important of which is the genus
Staphylococcus
Staphylococcus (from Greek: staphyl, bunch
of grapes and KKKO,
kkkos, granule) is a
genus of Gram positive bacteria
Under microscope they appear round (cocci)
and form in grape-like clusters
Staphylococcus
Staphylococcus
The Staphylococcus genus includes 32 species
and 8 sub-species
Members of this genus are facultatively
anaerobic, non-motile, Gram positive cocci
that usually form irregular clusters
They are catalase positive, oxidase negative,
ferment glucose, and have teichoic acid in
their cell walls
Staphylococcus
Organisms in clinical material may also appear
as single cells, pairs, or short chains
Most staphylococci are 0.5 - 1 m in diameter
Grow in medium containing 10% NaCl and at a
temperature ranging from 18 - 40C
Staphylococcus
Staphylococci are normally associated with
the skin, skin glands, and mucous membranes
of warm-blooded animals
Staphylococcus can cause a wide variety of
diseases in humans and other animals through
either toxin production or penetration
Staphylococcal toxins are a common cause of
food poisoning, as it can grow in improperly
stored food
Staphylococcus
Staphylococcus are responsible for many
human diseases
Staphylococcus cause a wide spectrum of life
threatening systemic diseases; infections of
the skin, soft tissues, bones, and urinary tract;
and opportunistic infections
Staphylococcus
Sometimes responsible for endocarditis and
infections of patients with lowered resistance
(e.g. wound infections, surgical infections,
urinary tract infections, body piercing)
S. aureus is the most important human
staphylococcal pathogen and cause boils,
abscesses, wound infections, pneumonia,
toxic shock syndrome, and other diseases
Classification
The main classification of staphylococci is
based on their ability to produce coagulase,
an enzyme that causes blood clot formation
2 classification:
Coagulase positive
Coagulase negative
Classification
Coagulase Positive
S. aureus - can produce coagulase
Some may be atypical in that they do not
produce coagulase
S. aureus is also catalase-positive, able to
convert hydrogen peroxide (H2O2) to water
and oxygen, which makes the catalase test
useful to distinguish staphylococci from
enterococci and streptococci.
Classification
Coagulase Positive
Inhabits and sometimes infects the skin of
domestic dogs and cats
Carry the genetic material that imparts
multiple bacterial resistance
Optical-rectalitis is caused by a coagulasepositive organism
Classification
Coagulase Negative
S. epidermidis - a commensal of the skin, but
can cause severe infections in
immunosuppressed patients and those with
central venous catheters
S. saprophyticus - part of the normal vaginal
flora, is predominantly implicated in
genitourinary tract infections in sexually-active
young women
Classification
Coagulase Negative
In recent years, several other Staphylococcus
species have been implicated in human
infections, notably S. lugdunensis, and S.
caprae
Common abbreviations for coagulase-negative
Staphylococcus species are CoNS and CNS
Alpha toxins
Beta toxins
Delta toxins
Gamma toxins
Panton-Valentine [P-V] leukocidin
Exfoliative toxins
Enterotoxins
Toxic Shock Syndrome Toxin-1 (TSST-1)
Bullous Impetigo
Endocarditis
Clinical Diseases
Staphylococcus epidermidis and other
coagulase-negative staphylococci
Endocarditis
Catheter and shunt infections
Prosthetic joint infections
Urinary tract infections
Laboratory Diagnosis
Microscopy - Gram positive cocci (clusters on
agar media and appear as single cells or small
groups in clinical specimens)
Culture - blood agar (haemolysis cause by
cytotoxins, particularly toxins)
Serology - antibodies to cell wall teichoic acids
Identification - biochemical tests (positive
reactions for coagulase [clumping factor],
heat-stable nuclease, alkaline phosphatase
and mannitol fermentation)
Question
What are the special characteristics of
Staphylococcus?
What are the virulence factors of
Staphylococcus?
What are the diseases that can be caused by
Staphylococcus?