Reagent Chemicals

Hydrogen Peroxide

Ninth Edition

H2O 2

ACS Specifications
Official from January 1, 2000
2000 American Chemical Society

Reagent: Hydrogen Peroxide

Formula Wt 34.01

CAS Number 7722841

NOTE. This reagent should be stored in a cool place in containers
with a vent in the stopper. The assay requirement applies to material stored properly for a reasonable time.

REQUIREMENTS
Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29.032.0% H2O2
MAXIMUM ALLOWABLE
Color (APHA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
Residue after evaporation . . . . . . . . . . . . . . . . . . . . . . . . 0.002%
Titrable acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0006 meq/g
Chloride (Cl). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 ppm
Nitrate (NO3) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 ppm
Phosphate (PO4). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 ppm
Sulfate (SO4). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 ppm
Ammonium (NH4) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 ppm
Heavy metals (as Pb) . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 ppm
Iron (Fe) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.5 ppm

TESTS
ASSAY. (By titration of the reductive capacity). Weigh accurately about 1 mL in a
tared 100-mL volumetric flask, dilute to volume with water, and mix thoroughly.
To 20.0 mL of this solution add 20 mL of dilute sulfuric acid (1 + 15) and titrate
with 0.1 N potassium permanganate. One milliliter of 0.1 N potassium permanganate corresponds to 0.01700 g of H2O2.
COLOR (APHA). (Page 19).
RESIDUE AFTER EVAPORATION. (Page 16). Evaporate 50 g (45 mL) to dryness in a tared porcelain or silica dish on a hot plate (100 C) and dry the residue
at 105 C for 30 min.
TITRABLE ACID. Dilute 10 g (9 mL) with 90 mL of carbon dioxide-free water.
Add 0.15 mL of methyl red indicator solution and titrate with 0.01 N sodium
hydroxide. The volume of sodium hydroxide solution consumed should not be
more than 0.6 mL greater than the volume required for a blank test on 90 mL of
the water used for dilution.
CHLORIDE, NITRATE, PHOSPHATE, AND SULFATE. (By ion chromatography, page 74). Obtain three 100-mL beakers (blank, test, standard) and add 10
mL water and 2.0 mg of sodium carbonate to each. Add 10.5 g (9.45 mL) of the
sample to the standard and test. To the standard, add 0.02 mg each of chloride,
nitrate, and phosphate ions and 0.05 mg of sulfate ion. To the blank, add 0.5 g
(0.45 mL) of the sample. Evaporate each to dryness on a steam bath or low-temperature hot plate. Dissolve the residues in a little water and dilute each to 25 mL.
Analyze 50-mL or 100-mL aliquots by ion chromatography and measure the various peaks. The differences between the sample and the blank should not be
greater than the corresponding differences between the standard and the sample.

Reagent Chemicals
Ninth Edition

ACS Specifications
Official from January 1, 2000
2000 American Chemical Society

Reagent: Hydrogen Peroxide

AMMONIUM. Add 0.1 mL of sulfuric acid to 2 g (1.8 mL) of the sample and
evaporate on a hot plate (100 C). Dissolve the residue in 45 mL of water and
add 3 mL of 10% sodium hydroxide reagent solution and 2 mL of Nessler reagent.
Any color should not exceed that produced by 0.01 mg of ammonium ion (NH4)
in an equal volume of solution containing the quantities of reagents used in the
test.
HEAVY METALS. (Page 28, Method 1). To 20 g (18 mL) in a 100-mL beaker add
about 10 mg of sodium chloride and evaporate to dryness on a hot plate (100
C). Dissolve the residue in about 20 mL of water and dilute with water to 25 mL.
IRON. (Page 30, Method 1). To 20 g (18 mL) in a 100-mL beaker, add about 10
mg of sodium chloride, and evaporate to dryness on a hot plate (100 C). Dissolve in 2 mL of hydrochloric acid, dilute with water to 50 mL, and use the solution without further acidification.