Part I

Results
Western Blot

The Western Blot (figure 1A) showed the expression of the protein tubulin at 0, 6, and 13

hours in samples of Artemia franciscana.

A B
Figure 1: Two samples obtained by performing gel electrophoresis on samples of Artemia franciscana at 3 life stages (0h, 6h, and 13h). Image A
is an western blot film from group I that shows the expression of the protein Tubulin (indicated by arrows), as well as the Molecular Weight
marker Kaleidoscope Precision Plus Protein™ Standards, or “K”. Image B shows a polyacramide gel stained with Coomassie Blue, and the MW
marker Bio-Rad Low Range Molecular Weight Standards “M”. This shows the various proteins of differing molecular weight that are present at
different stages of development of Artemia franciscana.

A B C
Figure 2: A) Distance travelled (mm) by 6 marker proteins from the Bio-Rad Low Range Molecular Weight Standards during gel
electrophoresis and the log of the markers’ known Molecular Weight (log kD). B) Artemia franciscana nauplius viewed through a
light microscope at the 10X objective, prepared wet mount on glass, unstained, with a drawing magnification of . C) Artemia
franciscana cyst viewed through a light microscope at the 10X objective, prepared wet mount on glass, unstained, with a drawing
magnification of .

Table 1: Distance travelled (mm) and corresponding molecular weight (kD) of proteins expressed in a sample of Artemia
franciscana at 0 hours, 6 hours, and 13 hours, as measured and calculated from a polyacramide gel stained with Coomassie Blue
that underwent electrophoresis.
Band 0 hour (mm) MW (kD) 6 hour (mm) MW (kD) 13 hour (mm) MW (kD)

1 11.5 109.65 13.5 91.20 13.5 91.20

2 14.5 85.11 15.0 81.28 16.0 74.13
3 15.5 77.62 16.5 72.44 18.5 60.26
4 17.5 66.07 18.5 60.26 19.5 56.23
5 18.5 60.26 19.5 56.23 20.5 51.29
6 20.0 53.70 20.5 51.29 21.5 47.86
7 21.0 48.98 22.0 45.71 23.0 41.69
8 22.5 43.65 23.0 41.69 25.5 33.88
9 24.5 37.15 25.5 33.88 28.5 26.30
10 29.5 24.55 30.5 22.39 30.0 23.44
 11 32.5 19.05 33.5 17.38 31.5 20.89
12 33.5 17.38 34.5 16.22 34.5 16.22

Part II
1. Artemia expresses tubulin in all stages of life.
2. The estimated molecular weight of tubulin is approximately 50 kD. We know this because
of the MW marker of known weight that travelled approximately the same distance as
tubulin (5th, blue, 50 kD).
3. To determine if tubulin is produced in Drosophila:
a. Grwgwe
b. Rwg
Part III

Langdon, C.M., P. Rafiee and T.H. MacRae. 1991. Synthesis of tubulin during early postgastrula
development of Artemia: isotubulin generation and translational regulation. Dev Biol. 148:138-
146.

Macrae, T.H., and R.F. Ludueña. 1984. Developmental and comparative aspects of brine shrimp
tubulin. Biochem J. 219:137-148.