Herbal Drugs Attenuated DN by ERK and TGF--signaling Pathway

Author :ZhouFei
Tutor:HeKaiYue
School :Nanjing Forestry University
CLC :R277.5
TYPE :Masters thesis
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Year:2011
Abstract:
In the process of our people battle with disease for thousands years of history, by practice, constant
awareness gradually accumulated a wealth of herbal knowledge. Chinese herba have unique
advantages on lasting effects and little side effect. The efficacy of many traditional Chinese medicine
not only withstood the test of long-term medical practice, but also has been confirmed by Sciences.
However, due to the complexity of the active ingredients of traditional Chinese medicine and doubt
the efficacy of Chinese herbal medicine, and mechanisms of many Chinese medicinal has not yet been
completely understanded, resulting in prevented the extensive promotion and application . Therefore,
it is extremely urgent to in-depth study on Chinese medicinal active ingredient and to explore its
mechanism.In this paper, screening effective herbal medicines from nine kinds of blood circulation
medicines in the cellular level, and then observed the effective of Chinese herbal on attenuated
diabetic nephropathy in rats by ERK,TGF-.After process of boiling, concentrated and freeze-dried
,ultimately, gaining the freeze-dried powder of nine kinds of medicinal plants;In vitro1) MTT assay:
nine blood circulation medicines of water extracts(except Giant Knotweed Rhizome) at lmg/ml have
no effects on cell proliferation, so can determine the scope of drug treatmentin the follow-up
experiments;2) Establishing cell models of over-expression extracellular matrix by add the rhTGF1;3) Use 2) have establishment of the model, treating a variety of blood circulation herbal water
extracts (final concentration 200g/ml)to cells, and then observating the expression of p-Smad2, and
found Lycopus can significantly reversed the increasing p-Smad2 protein that cause by rhTGF-1
stimulated, while the expression of Smad2 did not affect;4) Specific studies on water extract of
Lycopus affected on TR, TR, p-Smad2, Smad2, Smad4, Smad7, p-ERK1/2 and ERK1/2
protein expression, the study confirmed that rhTGF-lean significantly increasing expression. In the
latter experiments ,finding Lycopus can reverse the increasing expression of TR-, p-Smad2,
Smad4, p-ERK1/2 protein,but have no effects on Smad2TRand ERK1/2 and reduce Smad7.)
In vivo1) Successfully established a rat model of diabetic nephropathy experimental, that can provided
a guarantee for the follow-up studies;2) Although not lowering blood sugar, gaining the weight and
decreased urine volume, treatment group of water extract of Lycopus can significantly reducing the
STZ rats of SCr, BUN, urinary protein (P<0.05), and increased SOD activity (P<0.05); 3)The
reslut of RT-PCR show that Lycopus in the dose gropu (6g/kg/day) can significantly decreased the
mRNA of TGF-1 in the STZ rat; Western blot (WB) test results showed that all Lycopus treatment
group can significantly reduced p-Smad2 protein expression (P<0.01), but no effect on Smad2
protein expression.