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11 June 2014
Bacterial Morphology
Mary Ann Bunyi, M.D.
Walang iyo, Nicole. Akin ang asawa ko!!! Monica, The Legal Wife
Paulo Coelho
TOPIC OUTLINE
I. Bacterial Morphology
A. Bacterial Forms
II. Bacterial Cell Components
A. Cell Surface Layer
B. Appendages
C. Other Structures
2.Bacillus/ bacilli
May be long with square cut ends or short with
rounded ends
Coccobacilli very short bacilli appearing like
cocci
No cluster arrangement (Hemophilus influenzae)
Stain as gram negative (red)
3. Vibrio/ curved
Bacteria with a single turn, comma shaped
Stains gram negative (red)
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Bacterial
Morphology
4. Spiral/ spirillum
May be regular or irregular, with hook on one end
or both ends.
Some bacteria have to be visualized under special
microscope using a special stain.
Leptospira sp.
Cytoplasm
Cell Wall
Capsule
2. Appendages
Flagella
Pili
3. Other Structures
teichoic acid
inclusion bodies
spores
lipopolysaccharide
Cell Surface Layer
A. CYTOPLASM
o Total of everything inside the cytoplasmic space
o Main components
Proteins (mostly enzymes)
Ribosomes (composed of RNA and protein)sedimentation 70s (80s monomers) composed
of 30S and 50S subunit
o Other components include:
Cytoplasmic granules/ storage granules
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Bacterial
Morphology
GRAM POSITIVE
- thick walls (multilayer,
compact)
- almost exclusively
peptidoglycan
GRAM NEGATIVE
- thinner (1-2 layers)
- less compact
- composed of periplasmic
space
- outer membrane
1. Lipoproteins
most abundant
protein
lipid end inserted into
the outer membrane
protein end
covalently linked to
peptidoglycan
2. Phospholipids
form the outer
membrane matrix
and contributes to
outer membrane
stabilization
3. Porins
protein trimers that
form channels that
permit small
molecules to diffuse
across the membrane
4. Lipopolysaccharide
(LPS)
Lipid A endotoxin
toxic for humans and
may cause
hypotension, shock,
DIC, tissue necrosis
- more complex cell
envelope
- with transmembrane
proteins: integral and
porins
D. CAPSULE
o Closely surrounds the cell wall of some bacteria
o Well-defined mucoid polysaccharide structure
o Anti-phagocytic properties
prevent ingestion by WBCs
virulence factor for some bacteria
o Presence of antigenic diversity influence:
pathogenesis
effective vaccine preparation
ability to make a rapid and accurate diagnosis
o Roles:
protection from desiccation
extra source of nutrition
B. PILI
o Hairlike, rigid structures originating from the
cytoplasmic membrane
o Found predominantly in gram negative organisms
o Composed of structural protein subunits called
PILINS
o Two classes:
1. Sex pili
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Bacterial
Morphology
ENDOSPORE
o 2 most common are gram-positive rods: Bacillus
(obligately aerobic) and Clostridium (obligately
anaerobic)
o Also
occurs
in
Thermoactinomyces,
Sporolactobacillus, Sporosarcina, Sporotomaculum,
Sporomusa, and Sporohalobacter
o Sporulation - cycle of differentiation triggered by near
depletion of any of several nutrients (carbon, nitrogen,
or phosphorous)
o Spore
Resting cell
Highly resistant to desiccation, heat, and chemical
agents
Germinates to vegetative cell when returned to
favorable nutritional conditions
o Properties
Core-spore protoplast; contains chromosome
Spore wall
Cortex- thickest layer; with unusual type of
peptidoglycan which is extremely sensitive to
lysozyme
Coat -composed of keratin-like protein
Exosporium - lipoprotein membrane
SUMMARY
Bacteria consist of a unique cell wall, a cytoplasmic
membrane enclosing the cytoplasm, a nuclear apparatus,
ribosomes and various cytoplasmic granules. Some have
capsules, flagella, or pili.
1. Differences in the cell wall of gram positive and gram
negative exist and these account for the functional
properties as well as their susceptibility to
antimicrobial agents.
2. Capsules,
usually
polysaccharides,
closely
surrounding the cell wall or some bacteria. These
capsules may be antiphagocytic, vaccinogenic, and/for
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