Home Shop All Products Kits & Assays ELISA Kits ELISA TNF alpha ELISA Kit, Human

This product is only available through your local Thermo Scientific supplier. Learn where to buy >

TNF alpha ELISA Kit, Human

Catalog number: EH3TNFA
Therm o Scientific

Catalog
number

Related applications: Protein Assays and Analysis

Unit size

List price (INR)

EH3TNFA

96
assays

Learn where to buy

This product is only available through your local Thermo
Scientific supplier

EH3TNFA2

192
assays

Learn where to buy

This product is only available through your local Thermo
Scientific supplier

EH3TNFA5

480
assays

Learn where to buy

This product is only available through your local Thermo
Scientific supplier

Description
Assay TNF (human tumor necrosis factor alpha) levels in human serum, plasma
(EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo
Scientific Human TNF alpha ELISA Kit.

Specifications
Species:

Human

Product Size:

96 assays

Features of the Human TNF alpha ELISA Kit:

Protein Family:

Cytokines & Receptors

Target: natural and recombinant human TNF (human tumor necrosis factor
alpha)
Format: colorimetric ELISA (TMB substrate) in a clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 15.6 to 1000 pg/mL
Sensitivity: < 2 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and
heparin)
Total Assay Time: 3.5 hours
Sample Size: 50 L
Specificity: no significant cross-reaction with human IL-1, IL-1, IL-2, IL-3, IL-4, IL5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, TNF,
Eotaxin, RANTES, GRO, GRO, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, GCSF,
GMCSF, MIP-1, MIP-1, TGF, IFN, IFN mouse TNF rat TNF and bovine
TNF. TNF Receptor types 1 and 2 tested at 40 mg/mL did not interfere in this
assay. Recombinant pig TNF cross-reacted at 100%.

Detection Method:

Colorimetric

Research Category:

Immunology

This human TNF alpha ELISA kits uses pre-titered, matched pairs of coating and
detection antibodies to achieve sensitive and accurate measurement of tumor
necrosis factor alpha (TNF-alpha, TNF) in samples at 50 to 100 L per well. Also
included are calibrated recombinant protein standard, streptavidin-HRP, TMB
substrate solution and stop solution. The complete kit contains a precoated 96-well
strip plate and lot-optimized reagents. All components undergo quality control testing
View more
For Research Use Only. Not for use in diagnostic procedures.

Contents & storage

Pre-coated plate(s), standard or calibrator, detector
antibody, HRP conjugate, diluents, wash buffer,
substrate(s), and stop solution. Store kit at 2-8C. See
product manual for detailed contents and storage
conditions for maximum stability.

Figures

Human TNF alpha ELISA

standard curve

Documents
Manuals & protocols
User Guide: Human TNFalpha ELISA Kit

Material safety data sheets (MSDS)

TNF alpha ELISA Kit, Human

Certificates of analysis (COA)

Lot no. LD143877

Lot no. ME160213

Lot no. MH162551

Lot no. MI164216

Lot no. MJ162503

Lot no. NC168402

Lot no. NG175403

Lot no. OA183303

Lot no. OD185012

Lot no. OI190878

Lot no. PG206489

Lot no. PI208402

Search for COA by lot number:

Search

Search unsucessful? Request a COA

Citations & references

Human dendritic cells engineered to express alpha tumor necrosis factor maintain cellular maturation and T-cell stimulation capacity.
Ye Z, Chen Z, Sami A, El-Gayed A, Xiang J,
Cancer Biother Radiopharm (2006) 21:613-622
Product usage: In this study, we investigated the influence of different culture media (RPMI 1640/10% fetal calf serum [FCS] versus serum-free
AIM-V medium)on DC maturation and the change of maturation status of these ex vivo generated mature DCs during further culturing in medium
without infla

A possible role of NF-kappaB and HSP72 in skeletal muscle hypertrophy induced by heat stress in rats.
Ohno Y, Yamada S, Sugiura T, Ohira Y, Yoshioka T, Goto K
Gen Physiol Biophys 2010; (29):3 234-242

CD154 activates macrophage antimicrobial activity in the absence of IFN-gamma through a TNF-alpha-dependent mechanism.
Andrade RM, Wessendarp M, Subauste CS
J Immunol 2003; (171):12 6750-6756

View more

2015 Thermo Fisher Scientific Inc. All Rights Reserved.