GARY N.

LAVINE, PHD
Waltham, MA 02452
https://www.linkedin.com/in/garylavine

gary.lavine@rcn.com

PROJECT MANAGER, CMC DEVELOPMENT

Managing Multiple Projects while Controlling Costs
Extensive expertise in the Life Science and Biotechnology industries conducting projects to
increase efficiency and reduce costs. Known for managing multiple projects simultaneously in
ever-changing environment while meeting budget and time goals.
A self-managing leader skilled in creative problem solving and communicating effectively with a
wide variety of people. Able to establish collaborative relationships to quickly develop elegant
solutions.

PROFESSIONAL EXPERIENCE
PERKIN ELMER / CEIBA SOLUTIONS, Boston, MA

2014 2015

Senior Lab IT Consultant

Established new lifecycle management program for use by ~65 development scientists across 7
laboratories for a leading biotechnology company.
Lead adoption of pilot program and established new approach from the ground up for
managing scientific instrumentation across entire life cycle.
Measured program effectiveness by completing value-added analysis before and after
implementation to demonstrate scientists could save 80 hours annually.
Developed monthly dashboards for regular reporting of progress increasing transparency to
all stakeholders.
Identified two major risk factors to the success of the pilot project and developed an action
plan which increased adoption by end users to insure the program was implemented on an
ongoing basis.
WALTHAM CONSULTING, Waltham, MA

2010 2014

Principal Consultant
Consulted numerous biotechnology and pharmaceutical companies on efficient management of
scientific instrumentation and effective laboratory operations.
Reduced instrument maintenance costs 35% for mid-sized biotechnology company by
collaborating and consulting on complete inventory criticality assessment and renegotiating
entire maintenance program.
Self-published report Lessons from Bostons Best Laboratories, providing laboratory
leaders with novel approaches to improve overall laboratory performance.
Gathered and documented requirements for clinical production LIMS program and configured
the LIMS software, meeting all project goals.
Chartered and collaborated with stakeholders, to plan a well-received annual lab productivity
micro-conference for 3 consecutive years.

GARY N. LAVINE

gary.lavine@rcn.com

ALNYLAM PHARMACEUTICALS, Cambridge, MA

PAGE TWO
2003 2010

Senior Scientist
2007 2010
Worked as bench scientist primarily on analytical issues relating to development of siRNA
therapeutics.
Reduced maintenance cost 10% while shortening instrument repair time by establishing and
managing maintenance and repair program for all scientific instrumentation.
Developed procedure for unambiguous confirmation of short oligionucleotide sequences and
performed procedure on variety of sequences, increasing reliability of confirmation with both
coverage map and reliability score for use in CMC sequence confirmation
Chartered a project to develop efficient mass spectrometric identification of oligionucleotide
impurities separated by ion exchange chromatography, improving understanding of impurity
profile.
Scientist
2003 2007
Selected as companys first analytical chemist to develop initial methods for analysis of siRNA,
lipids, and sugars used as potential delivery vehicles.
Developed program for in-house monitoring of oligionucleotide quality and impurity
identification to support in-house synthesis, ensuring high quality material used by biologists
in research programs.
Resolved standard purity assay issue by recognizing problem and quickly developing
alternative HPLC protocol.
Ran cGMP analysis on novel lipids to support in-house production effort, meeting aggressive
timeline.
BEYOND GENOMICS, Waltham, MA

2001 2003

Senior Scientist
Worked as bench chemist doing analytical development.
Managed development effort of ion mobility time of flight mass spectrometry (IMS-TOF) on
prototype instrument, documenting and demonstrating instrument capabilities in poster
publications.
Developed approaches to medium throughput proteomics with multi-dimensional liquid
chromatography, adopted as company standard method.
Participated in redesign of corporate employee performance evaluation system, resulting in
streamlined system.

EDUCATION

Doctor of Philosophy (PhD), Chemistry, Michigan State University, East Lansing, MI

Master of Science (MS), Analytical Chemistry, Wright State University, Dayton, OH
Bachelor of Arts (BA), Chemistry, The College Of Wooster, Wooster, OH

PATENT

M. Kretschmer, G. Lavine Method for Confirming Chemically Modified Nucleic Acid

Sequences Alnylam pending patent application

PUBLICATIONS
1. H. Addepalli, Meena, C.G. Peng, G. Wang, Y. Fan, K. Charisse, K.N. Jayaprakash,
K.G. Rajeev, et al. Modulation of thermal stability can enhance the potency of
siRNA. Nucleic Acids Res. 2010 Jul 7. [Epub ahead of print]
2. M. Kretschmer, G. Lavine, J. McArdle, S. Kuchimanchi, V. Murugaiah, M.
Manoharan, An Automated Algorithm for Sequence Confirmation of Chemically
Modified Oligonucleotides by Tandem Mass Spectrometry ANALYTICAL
BIOCHEMISTRY 405(2) 213-223
3. V. Murugaiah, W. Zedalis, G. Lavine, K. Charisse, M. Manoharan, ReversedPhase High Performance Liquid Chromatography Method for Simultaneous Analysis
of Two Liposome-Formulated Short Interfering RNA Duplexes ANALYTICAL
BIOCHEMISTRY 401(1) 61-67
4. J. Soutschek, A. Akinc, B. Bramlage, K. Charisse, R. Constien, M. Donoghue, S.
Elbashir, A, Geick, et al., Therapeutic Silencing of an Endogenous Gene by
Systemic Administration of Modified siRNAs NATURE MEDICINE 11(9, suppl.) 25-30
5. C. Clish, E. Davidov, M. Oresic, T. Plasterer, G. Lavine, T. Londo, M. Meys, P.
Snell, et al., Methods for the Differential Integrative Omic Analysis of Plasma from
a Transgenic Disease Animal Model Omics 8(4) 267-288
6. G. Lavine and J. Allison, Evaluation of Bumetanide as a Matrix for Prompt
Fragmentation/Post-Source Decay Matrix Assisted Laser Desorption/Ionization Mass
Spectrometry JMASS SPECTROM 34: (7) 741-748
7. J. Strahler, Y. Smelyanskiy, G. Lavine, Z.H. Huang, and J. Allison, Using ChargeDerivatized Peptides in Direct Analysis from Polyacrylamide Gels and Membranes
using Matrix- Assisted Laser Desorption/ionization Mass Spectrometry INT J MASS
SPECTROM l69: 111-126

PRESENTATIONS AT SCIENTIFIC MEETINGS

1. .G. Lavine, M. Kretschmer,, J. McArdle, S. Kuchimanchi, V. Murugaiah, M. Manoharan,
Novel Method for the Confirmation of siRNA Sequence by LC-MS/MS Abstracts of Papers,
240th ACS National meeting Boston, MA, 2010
2. V. Murugaiah, W. Zedalis, G. Lavine, K. Charise, M. Manoharan, Development of a StabilityIndicating, Ion-Pair RP-HPLC Method for Seperation and Quantitative Determination of two
siRNA Duplexes in a liposome Abstracts of Papers, 240th ACS National meeting Boston, MA,
2010
3. H. Addepalli, Meena,CG. Peng, G. Wang, Y. Fan, K. Charisse, KN. Jayaprakash, KG.
Rajeev, et al. Modulation of thermal stability can enhance the potency of siRNA. Abstracts of
Papers, 240th ACS National meeting Boston, MA, 2010
4. R.K. Pandey, J. Xia, G. Lavine, A. Akinc, R.G. Kallanthottathil, M. Manoharan, Effect of
Ligand Tethered 2,4-Difluorotoluyl ribonucleosides on RNA Interference Abstracts of Papers,
240th ACS National meeting Boston, MA, 2010
5. G. Lavine, S. Valentine, T. Londo, S. Naylor, Using Ion Mobility/Time-of-Flight Mass
Spectrometry for Comparative Proteomics Proceedings of the 51st ASMS Conference on Mass
Spectrometry and Allied Topics, Montreal, QC, 2003
6. W. Stochaj, M. Meys, G. Lavine, P. Snell, A Comparison of Proteolytic Digestion Methods for
use in the Preparation of Soluble Protein Samples Prior to Proteomic Analysis Proceedings of
the ABRF conference, Denver, CO 2002
7. G. Lavine, S. Valentine, T. Londo, X. Zhang, S. Naylor, Using Ion Mobility/Time-of-Flight

Mass Spectrometry in the -Omics Revolution Proceedings of the 50th ASMS Conference on
Mass Spectrometry and Allied Topics, Orlando, FL, 2002
8. G. Lavine and H. M. Fales, Using Maximum Entropy in the Interpretation of Fragmentation
Spectra of Highly Charged Peptides and Proteins Proceedings of the 49th ASMS Conference on
Mass Spectrometry and Allied Topics, Chicago, IL, 2001
9. G. Lavine and H. M. Fales, Controlling the Charge State of Peptides in
Electrospray Ionization Mass Spectrometry Proceedings of the 48th ASMS
Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, 2000
10. G. Lavine and J. Allison, Improvements in the Technique of Prompt
Fragmentation ill MALDI-TOF MS of Peptides Proceedings of the 46th ASMS
Conference on Mass Spectrometry and Allied Topics, Orlando, FL, 1998
11. J.T.Watson, Z.H. Huang, N. Xu, G. Lavine, J. Allison, and D. A. Gage,
New Matrices for MALDI-MS Eastern Analytical Symposium, Somerset, NJ, 1997
12. J.Strahler, Y. Smelyanskiy, G. Lavine, and J. Allison, Charge Derivation of
Peptides for their Direct Analysis from Polyacrylamide Gels and Membranes
Using Matrix-Assisted Laser Desorptjon/lonization Mass Spectrometry.
Proceedings of the 45th ASMS Conference on Mass Spectrometry and Allied
Topics, Palm Springs, CA, 1997
13. G. Lavine and J. Allison, Post Source Decay Studies of Prompt Fragment
ions from Peptides Formed in MALDI-TOFMS Proceedings of the 45th ASMS
Conference on Mass Spectrometry and Allied Topics, Palm Springs, CA, 1997
14. C. Uzelmeier, D. Bercik, G. Lavine, R. Marcus, M.J. Waner, Science Theatre: A Student
Outreach Organization Abstracts of Papers, 215th ACS National meeting Dallas, TX, 1998
15. M.J. Waner, J. Bier, G. Lavine, R. Marcus, R. Martin, A Hands on DNA Workshop for High
School Students Abstracts of Papers, 215th ACS National meeting Dallas, TX, 1998
16. J.T.Watson, Z.H. Huang, P.C. Liao, J. Wu, K. Roth, G. Lavine, D. A. Gage, and J. Allison,
TMPP derivatives: A New Generation of Charged Derivatives For Desorption/lonization:
Proceedings of the 44th ASMS Conference on Mass Spectrometry and Allied Topics, Portland,
OR, 1996
17. G. Lavine and J. Allison, Scanning Laser Power While Acquiring MALDI
Spectra -Does Prompt Fragmentation Occur at High Power Proceedings of the 44th ASMS
Conference on Mass Spectrometry and Allied Topics, Portland, OR, 1996
18. J. Allison, P.C. Liao, G. Lavine, and J. Hiller, Dissecting MALDI-TOF Mass Spectra
Proceedings of the 43rd ASMS Conference on Mass Spectrometry and Allied Topics, Atlanta,
GA, 1995
19. J. Hiller, G. Lavine, G. Szekely, and J. Allison, The behavior of peptide Mixtures in MALDIMS Proceedings of the 43rd ASMS Conference on Mass Spectrometry and Allied Topics,
Atlanta, GA, 1995
20. G. Lavine and J. Allison, Influence of Sample Preparation on Time- Dependent MALDI MS
of Proteins Proceedings of the 43rd ASMS Conference on Mass Spectrometry and Allied
Topics, Atlanta, GA, 1995

INVITED TALKS
1. CMC Analytical Analysis of siRNA Biotechnology program, Northeastern University, Boston,
MA, October 2007
2. An Example of How the University Reaches Out to the Community Sigma
Xi Congressional Staff Visit, Michigan State University, East Lansing. MI, August 1997
3. Innovative Outreach Activities, Sigma Xi Annual Meeting, New Orleans, LA, November 1996