Professional Documents
Culture Documents
Fluid Cleanliness
Awareness Training
GE Australia, November 2010
Presented by
Mohammed Ali
Ken McKenzie
Country Manager
Introduction
Particle Analysis & Contamination Control
Why Particle Counting is Carried Out?
What are Particles?
Automatic Particle Counters vs Optical
Microscopes
Fluid Cleanliness Testing
Review & Conclusion
Introduction
Particle Analysis & Contamination Control
Why Particle Counting is Carried Out?
What are Particles?
Automatic Particle Counters vs Optical
Microscopy
Fluid Cleanliness Testing
Review & Conclusion
Time is Money !
Introduction
Particle Analysis & Contamination Control
Why Particle Counting is Carried Out?
What are Particles?
Automatic Particle Counters vs Optical
Microscopes
Fluid Cleanliness Testing
Review & Conclusion
Introduction
Particle Analysis & Contamination Control
Why Particle Counting is Carried Out?
What are Particles?
Automatic Particle Counters vs Optical
Microscopes
Fluid Cleanliness Testing
Review & Conclusion
Slide Preparation
Slides Prepared in a Clean Room
Ensure that all Equipment making contact
with the Fluid Sample is Flushed Clean
Do Not Breath on or Lean Over Fluid Sample
or test Equipment
Optical Microscope
100x Magnification
1 Division = 10 m
Particle Size = 40 m
Particle Counting
Count is Performed to ARP 598 with Class
reported by as either AS or NAS Level
Examine Areas Over Whole Membrane Do
Not Concentrate on the Middle Few Squares
Particle Size
>100 m
>50 m
>25 m
>15 m
> 5 m
Count Particles
Whole Membrane
20 Grid Squares
20 Grid Squares
10 Grid Squares
10 Unit Areas
Magnification
x50
x50
x100
x100
x200
Particle Counting
Particle Distribution should be Uniform,
Uneven Distribution Will Invalidate the Count
Particle Size guided by its Largest Dimension
i.e. Fibre Length can be more than 10x its
Width
Error in Counting Should not be Greater than
10% between Operators or Laboratories
Particle Counting
Count for 100ml Fluid & Whole membrane
Factor needed to convert Count from Area
Examined to Whole membrane
Area Examined Area mm2
Factor
Whole Membrane
960
x1
1 Grid Square
~9.5
x100
10 Grid Squares
~95
x10
20 Grid Squares
~190
x5
1 Unit Area
Microscope dependent
Particle Counting
Count for 100ml Fluid & Whole membrane.
Factor needed to convert Count from Volume
tested to 100ml.
Fluid Volume
100
50
25
Factor
x1
x2
x4
Cleanliness Classes
NAS1638 (Old Industry Standard)
Particles Counted according to Size Ranges:
5-15, 15-25, 25-50, 50-100 & >100 m
Cleanliness Class generally dictated by larger
particles
AS4059
Cumulative Count based on Particle Size:
>1, >5, >15, >25, >50 & >100 m
Numeric Class qualified by Letter Code based on
Particle Sizes counted
NAS 1638
Size
Optical Count
Class
00
0
1
2
3
4
5
6
7
8
9
10
11
12
5-15 m
125
250
500
1000
2000
4000
8000
16000
32000
64000
128000
256000
512000
1024000
4
8
16
32
63
126
253
506
1012
2025
4050
8100
16200
32400
1
2
3
6
11
22
45
90
180
360
720
1440
2880
5760
> 100m
0
0
1
1
2
4
8
16
32
64
128
256
512
1024
AS4059
Size
Optical Count
Size Code
000
00
0
1
2
3
4
5
6
7
8
9
10
11
12
> 1m
A
195
390
780
1560
3120
6520
12,500
25,000
50,000
100,000
200,000
400,000
800,000
1,600,000
3,200,000
> 100m
F
0
0
0
1
1
2
4
8
16
32
64
128
256
512
1,020
Troubleshooting
Poor or Erratic Cleanliness Results?
Is Your Room Clean?
Is Your Technique Good Enough?
Has a Background/Blank Contamination Count
been made?
Fluid Transport Issues?
Blank Analysis
Prepare a Blank Membrane Slide Using 100
5ml Filtered Water
Total Count should be <10% of Test Fluid
Sample Levels