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Department of Pediatrics, Brown Medical School, Providence, Rhode Island; bDepartment of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana;
Center for Research for Mothers and Children, National Institute of Child Health and Human Development, Rockville, Maryland; dRTI International, Research Triangle
Park, North Carolina
c
The authors have indicated they have no nancial relationships relevant to this article to disclose.
ABSTRACT
OBJECTIVE. Beneficial effects of breast milk on cognitive skills and behavior ratings
have been demonstrated previously in term and very low birth weight infants.
Extremely low birth weight infants are known to be at increased risk for developmental and behavior morbidities. The benefits of breast milk that is ingested in
the NICU by extremely low birth weight infants on development and behavior
have not been evaluated previously.
METHODS. Nutrition data including enteral and parenteral feeds were collected prospectively, and follow-up assessments of 1035 extremely low birth weight infants
at 18 months corrected age were completed at 15 sites that were participants in
the National Institute of Child Health and Human Development Neonatal Research
Network Glutamine Trial between October 14, 1999, and June 25, 2001. Total
volume of breast milk feeds (mL/kg per day) during hospitalization was calculated.
Neonatal characteristics and morbidities, interim history, and neurodevelopmental
and growth outcomes at 18 to 22 months corrected age were assessed.
RESULTS. There were 775 (74.9%) infants in the breast milk and 260 (25.1%) infants
in the no breast milk group. Infants in the breast milk group were similar to those
in the no breast milk group in every neonatal characteristic and morbidity,
including number of days of hospitalization. Mean age of first day of breast milk for
the breast milk infants was 9.3 9 days. Infants in the breast milk group began to
ingest non breast milk formula later (22.8 vs 7.3 days) compared with the non
breast milk group. Age at achieving full enteral feeds was similar between the
breast milk and non breast milk groups (29.0 18 vs 27.4 15). Energy intakes
of 107.5 kg/day and 105.9 kg/day during the hospitalization did not differ between
the breast milk and non breast milk groups, respectively. At discharge, 30.6% of
www.pediatrics.org/cgi/doi/10.1542/
peds.2005-2382
doi:10.1542/peds.2005-2382
Key Words
human milk, extremely low birth weight,
Bayley, outcomes
Abbreviations
LC-PUFAlong-chain polyunsaturated
fatty acids
ELBW extremely low birth weight
AAPAmerican Academy of Pediatrics
CA corrected age
NICHDNational Institute of Child Health
and Human Development
BSID IIBayley Scales of Infant
Development II
BRSBehavior Rating Scale
MDIMental Development Index
PSIPsychomotor Development Index
VLBWvery low birth weight
Accepted for publication Jan 31, 2006
Address correspondence to Betty R. Vohr, MD,
Women and Infants Hospital, 101 Dudley St,
Providence, RI 02905. E-mail: Betty_Vohr@
brown.edu
PEDIATRICS (ISSN Numbers: Print, 0031-4005;
Online, 1098-4275). Copyright 2006 by the
American Academy of Pediatrics
e115
VOHR et al
METHODS
The study cohort was derived from the 1433 infants who
were enrolled prospectively in the Glutamine Trial36 at
15 sites of the National Institute of Child Health and
Human Development (NICHD) Neonatal Research Net-
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Breast Milk
No Breast Milk
775 (74.9)
425 (55)
785 129
26.5 2
84 (11)
29 (4)
54 (7)
6 (1)
311 (40)
349 (46)
99 42
260 (25.1%)
140 (54)
794 133
26.7 2
25 (10)
10 (4)
26 (10)
3 (1)
91 (35)
120 (47)
96 41
.781
.363
.331
.586
.931
.113
.568
.142
.714
.301
Data are mean SD or n (%). IVH indicates, intraventricular hemorrhage; PVL, periventricular
leukomalacia.
a Bronchopulmonary dysplasia was dened as oxygen administration at 36 weeks postmenstrual age.
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VOHR et al
Breast Milk
No Breast Milk
775 (74.9)
260 (25.1)
113 (15)
363 (47)
299 (39)
387 (50)
45 (17)
128 (49)
87 (33)
78 (30)
206 (28)
197 (27)
162 (22)
163 (22)
253 (34)
83 (35)
94 (40)
35 (15)
15 (6)
43 (18)
303 (39)
325 (42)
133 (17)
14 (2)
280 (42)
2.1 1.3
167 (64)
71 (27)
18 (7)
4 (2)
127 (59)
2.7 1.8
.2793
.001
.0001
.001
.0001
.001
.001
Data are n (%) except where indicated. HMO indicates health maintenance organization.
Adjusted P
MDI
MDI 85
PDI
PDI 85
BRS
Orientation/engagement
Emotional regulation
Motor quality
Total
Blind in both eyes
Hearing aids
Cerebral palsy (moderate/severe)
Rehospitalizations before rst birthday
79.9 18
421 (58.1)
84.6 19
307 (42.9)
75.8 16
168 (70.9)
81.3 17
116 (49.2)
.0709
.0355
.0227
.0939
53.8 30
53.0 29
50.1 35
52.0 31
4 (0.5)
9 (1.2)
43 (5.6)
180 (23.3)
48.5 29
47.1 29
44.1 33
45.6 30
0 (0.0)
4 (1.6)
16 (6.2)
78 (30.1)
.0351
.0825
.0296
.0280
.5773a
.7472a
.7327a
.028
Adjustments were made for the following confounders: mothers age, education, marital status, and race and infants gestation, gender, sepsis,
IVH 3 to 4, PVL, O2 at 36 weeks, necrotizing enterocolitis, weight 10th percentile at 18 months, and volume of breast milk (mL/kg per day) for
every day of hospitalization (a continuous variable).
a Unadjusted P.
Parameter
Estimate
0.53
0.63
0.82
.0002
.0001
.0025
Logistic regression
OR
95% CI
0.94
0.900.98
Estimates are adjusted for standard covariates listed previously. OR indicates odds ratio; CI,
condence interval.
TABLE 5 Breast Milk Feeding Measures, Developmental Outcomes, and Rehospitalization by Quintiles of Breast Milk Intake During
Hospitalization
No Breast Milk
Total breast milk (mL/kg per day) for every day of hospitalization
Total breast milk (mL/kg per day) on days breast milk received
% Discharged on breast milk
Mean MDI score
Mean PDI score
Mean total BRS percentile score
Rehospitalized 1 y
a Value
0.0
0.0
0.0
75.8
81.3
45.6
30.2
Percentile
Adjusted P
20th
20th40th
40th60th
60th80th
80th
1.0
22.1
0.7
74.2
80.6
44.8
25.2
7.3
45.0
2.1
76.9
82.7
52.1
32.2
24.0
66.8
8.6
78.3
84.2
50.1
26.0
63.8
95.3
40.2
80.4
84.4
51.8
23.2
110.6
124.0
85.1
87.3a
89.4a
58.8a
12.7a
.0044
.0027
.0281
.0460
e119
BRS, and rehospitalization at the 40th to 60th percentiles and the 60th to 80th percentiles trended toward
significant.
DISCUSSION
ELBW infants are known to be at increased risk for
developmental delays, neurosensory impairments, poor
growth, and rehospitalization after discharge. The primary objective of perinatal and neonatal studies of
ELBW infants has been to identify interventions that
improve these outcomes. Breast milk has been shown
consistently to have beneficial effects for term infants.
Studies in premature infants have shown both positive
effects16,810,39 and no effects40 on cognitive outcome and
positive effects on behavior ratings.1113
Our study to investigate the effects of breast milk on
BSID II scores and rehospitalization would be difficult to
assess in a breast milk/no breast milk randomized trial
because method of feeding is determined by the mother.
In addition, the AAP recommends breast milk for premature infants; therefore, there is no true equipoise. In
the studies of Lucas et al,16 randomization to study
group was made after the mothers made the choice to
bottle or breastfeeding. These studies compared breast
milk in various combinations to term formula, preterm
formula, and banked breast milk. The prospective detailed daily neonatal enteral, including breast milk, and
parenteral nutritional intake data that were collected
longitudinally as part of the NICHD Network Glutamine
Trial36 on a large population of ELBW infants who were
assessed at 18 to 22 months CA allowed us to examine
the relationship between breast milk and outcome. The
nutritional intake data revealed that 75% of the infants
received some breast milk during their NICU stay and
30.6% of infants were still receiving breast milk at the
time of discharge.
Consistent with previous reports,41,42 mothers who
provided breast milk were more likely to be married, be
better educated, have private health insurance, have
higher income, and have lower parity. Black mothers
were less likely to provide breast milk. It is of interest
that there were no differences in any of the neonatal
characteristics or morbidities compared in bivariate analysis. This is similar to an NICHD Network study43 that
evaluated the relationship between late-onset sepsis and
breast milk. The bivariate relationship between any
breast milk feeding and sepsis versus no sepsis was not
significant. However, on multivariate logistic regression,
it was identified that for every 10% increase in breast
milk, there was a 5% decrease in the rate of sepsis.
Furman et al44 also reported a dose relationship between
increasing breast milk ingestion and decreasing rate of
sepsis.
Despite the limited amount of breast milk ingested
during the NICU hospitalization, our results support our
hypothesis that breast milk ingestion in the NICU by
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VOHR et al
e121
M. Bethany Ball, BS; University of Tennessee at Memphis (U10 HD21415): Sheldon B. Korones, MD*, Henrietta Bada, MD, Tina Hudson, RN; University of Texas
Health Science Center at Houston (U10 HD21373): Jon
E. Tyson, MD, MPH*, Brenda Morris, MD, Georgia
McDavid, RN; University of Texas Southwestern Medical
Center (U10 HD40689): Abbot R. Laptook, MD*, Sue
Broyles, MD, Susie Madison, RN, Jackie Hickman, RN;
Wayne State University (U10 HD21385): Seetha Shankaran, MD*, Yvette Johnson, MD, Geraldine Muran,
RN, Debbie Kennedy, RN; Women and Infants Hospital
(U10 HD27904): William Oh, MD*, Betty Vohr, MD,
Angelita Hensman, RN, Lucy Noel, RN; Yale University
(U10 HD27871, M01 RR 06022): Richard A. Ehrenkranz, MD*; Patricia Gettner, RN, Elaine Romano, RN;
University of Alabama at Birmingham (U10 HD34216):
Waldemar A. Carlo, MD*, Myriam Peralta, MD, Monica
V. Collins, RN, Vivien Phillips, RN. *Principal investigator; follow-up principal investigator.
REFERENCES
1. Lucas A, Morley R, Cole TJ, Gore SM. A randomised multicentre study of human milk versus formula and later development
in preterm infants. Arch Dis Child Fetal Neonatal Ed. 1994;70:
F141F146
2. Lucas A, Morley R, Cole TJ. Randomised trial of early diet in
preterm babies and later intelligence quotient. BMJ. 1998;317:
14811487
3. Lucas A, Fewtrell MS, Morley R, et al. Randomized outcome
trial of human milk fortification and developmental outcome
in preterm infants. Am J Clin Nutr. 1996;64:142151
4. Lucas A, Gore SM, Cole TJ, et al. Multicentre trial on feeding
low birthweight infants: effects of diet on early growth. Arch Dis
Child. 1984;59:722730
5. Lucas A, Morley R, Cole TJ, Lister G, Leeson-Payne C. Breast
milk and subsequent intelligence quotient in children born
preterm. Lancet. 1992;339:261264
6. Lucas A, Morley R, Cole T, Gore S. A randomized multicenter
study of human milk versus formula and later development in
preterm infants. Arch Dis Child. 1994;71:288 290
7. Horwood LJ, Darlow BA, Mogridge N. Breast milk feeding and
cognitive ability at 7 8 years. Arch Dis Child Fetal Neonatal Ed.
2001;84:F23F27
8. Gale CR, Martyn CN. Breastfeeding, dummy use, and adult
intelligence. Lancet. 1996;347:10721075
9. Pollock JI. Mothers choice to provide breast milk and developmental outcome. Arch Dis Child. 1989;64:763764
10. Jacobson SW, Jacobson JL, Dobbing J, Beijers RJW. Breastfeeding and intelligence. Lancet. 1992;339:926
11. Hart S, Boylan LM, Carroll S, Musick YA, Lampe RM. Brief
report: breast-fed one-week-olds demonstrate superior neurobehavioral organization. J Pediatr Psychol. 2003;28:529 534
12. Horne RS, Parslow PM, Ferens D, Watts AM, Adamson TM.
Comparison of evoked arousability in breast and formula fed
infants. Arch Dis Child. 2004;89:2225
13. Ooylan LM, Hart S, Porter KB, Driskell JA. Vitamin B-6 content of breast milk and neonatal behavioral functioning. J Am
Diet Assoc. 2002;102:14331438
14. Hylander MA, Strobino DM, Dhanireddy R. Human milk feedings and infection among very low birth weight infants. Pediatrics. 1998;102(3). Available at: www.pediatrics.org/cgi/
content/full/102/3/e38
e122
VOHR et al
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
57.
e123