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Diamino
For extraction, the European standard EN 15662 recommends a citrate extraction mix (Mix I), while AOAC
standard 2007.1 uses an acetate extraction mix (Mix II).
10 g sample are homogenized with 10 mL acetonitrile. After adding the internal standard the sample is shaken with 4 g MgSO4
and 1 g NaCl and afterwards centrifuged.
1 mL of the supernatant is spiked with 25 mg CHROMABOND
Diamino and 150 mg MgSO4 and shaken again. After centrifugation the supernatant is injected into GC/MS.
For optimizing the extraction of pH-dependent compounds, for minimizing decomposition of sensitive
substances, and for broadening the matrix spectrum,
different modifications of the QuEChERS method have
been elaborated.
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Recommended application:
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Composition
CHROMABOND
Pack of
730970
50
730971
50
730972
50
730973
50
730975
50
730974
50
730561
730562
50
30
730653.20
730653
20 g
100 g
730223
50
15 mL* Mix I
15 mL* Mix II
6
4
REF
4 g MgSO4, 1 g NaCl, 0.5 g Na2H citrate 1.5 H2O, 1 g Na3 citrate 2 H2O
6 g MgSO4, 1.5 g Na acetate
15 mL* Mix IV
15 mL* Mix V
15 mL* Mix VI
QuEChERS mixes
Sample property
Low fat content
Moderate content of chlo(e.g., apples, strawberries) rophyll and carotinoids
(e.g., carrots, lettuce)
Citrate extraction
Mix I
Citrate extraction
Mix I
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Diamino/Carbon clean-up
Mix IV
Diamino/Carbon clean-up
(higher Carbon content)
Mix V
Diamino/C18 ec clean-up
Mix VI
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Code
Matrix
Modification / Application
Similar phases*
SA
silica
PCA
silica
PSA
HR-XC
silica
PS/DVB
HR-XA
PS/DVB
HR-XCW
PS/DVB
HR-XAW
PS/DVB
PS-OH
PS-H+
PS-Mix
PS-Ag+
PS-Ba2+
PS/DVB
PS/DVB
PS/DVB
PS/DVB
PS/DVB
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Na2SO4
silica
Strata Screen-C Bond Elut Certify I DSCMCAX Clean Screen DAU Accubond Evidex
Bakerbond Narc-2 Isolute HCX LiChrolut
TSC HyperSep Verify CX
Strata Screen-A Bond Elut Certify II Clean
Screen THC Bakerbond Narc-1 Isolute
HAX HyperSep Verify AX
silica
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