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catalog
HPLC
GC
TLC
Syringe filters
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How to contact us
Chromatography
Our customers can count on competent and reliable service all over the world:
Branches in France, Switzerland, and the United States with scientifically educated staff
Globally operating network of qualified and specially trained distributors in more than 150 countries
For a complete list of branches and authorized distributors see www.mn-net.com/distributors.
Germany and international
MACHEREY-NAGEL GmbH & Co. KG
Neumann-Neander-Str. 68
52355 Dren
phone
+49 24 21 969-0
toll-free
0800 2616 000
fax
+49 24 21 969-199 oder -198
e-mail
info@mn-net.com
France
MACHEREY-NAGEL EURL
USA
MACHEREY-NAGEL Inc.
info@mn-net.com
Since 1911 MACHEREY-NAGEL has represented high quality, innovation and reliability in chemical and
biomolecular analysis. Friendly expert advice for our highly valued customers as well as outstanding product
quality have been the cornerstones of our corporate success for more than 100 years. CEO C. Wagner, the greatgranddaughter of the companys founder, has been managing the enterprise since 2000.
Our product ranges:
Filtration
Rapid Tests
Water Analysis
Chromatography
Bioanalysis
1961:
MACHEREY-NAGEL
becomes one of the
pioneers in TLC.
1970:
Expansion of the
product range by column
chromatography
1974:
NUCLEOSIL one of the first
spherical HPLC silicas leads to
our core competence in silica
technology
1982:
first fused silica capillary
columns for GC
4
7
3
6
5
1987:
CHROMABOND
columns for SPE
160
0
1994:
OPTIMA
capillary columns
for optimum
GC separations
www.mn-net.com
278
10
328
20
370
30
C
40
min
2002:
NUCLEODUR
high purity
spherical
silica
for HPLC
NUCLEODUR
NUCLEOSHELL
2011:
NUCLEOSHELL core
shell silica for highest
efficiency in HPLC
Chromatography
1952:
MN launches the first
products for paper
chromatography. In the
same year, Martin and
Synge receive the Nobel
Prize in Chemistry for
the development of
partition chromatography.
www.mn-net.com
Chromatography
info@mn-net.com
Catalog contents
Solid Phase Extraction and Flash chromatography
CHROMABOND phases and accessories for SPE
High-throughput SPE and Flash chromatography
Sample clarification
Liquid chromatography
Gas chromatography
Appendices
www.mn-net.com
6676
77103
103108
110197
198206
208234
236281
282291
293297
298327
Chromatography
152
5364
Sample preparation
Page
68
info@mn-net.com
NUCLEOSHELL RP 18
Key features:
Suitable for LC/MS and HPLC at pH extremes (pH 111)
Superior base deactivation, ideal for method development
Recommended application:
Overall sophisticated analytical separations,
e.g., analgesics, anti-inflammatory drugs, antidepressants;
herbicides; phytopharmaceuticals; immunosuppressants
USP L1
H 3C
Page
148
s
en
rb
So
HILIC
IC
aly
An
NPC
Eluent
RPC
www.mn-net.com
N
O
Key features:
Hydrophobic phase with alternative selectivity in comparison to
classical C18 modifications
Separation principle based on 4 retention mechanisms (polar interactions (Hbonds), dipole-dipole interactions, - interactions,
hydrophobic interactions)
Recommended application:
Aromatic and unsaturated compounds, phenols, halogenated compounds, isomers, polar compounds like pharmaceuticals, antibiotics;
high retention of basic compounds
USP L43
NUCLEOSHELL HILIC
CH3
Si(CH3)3
O
O
F
H
F
e
Pag
150
zwitterionic phase
Key features:
Ideal for reproducible and stable
Page
chromatography of highly polar analytes
152
Very short column equilibration times
Recommended application:
Hydrophilic compounds such as organic polar acids and bases, polar
natural compounds, nucleosides, oligonucleotides, amino acids,
peptides, water soluble vitamins
HPLC
NUCLEOSHELL PFP
Pa
14 ge
4
Key features:
Excellent base deactivation
Suitable for LC/MS and stable in 100% aqueous mobile phases
Pronounced steric selectivity
Recommended application:
Exceptional selectivity for phenols and nitrogen containing compounds, polar compounds like basic pharmaceuticals, organic
acids, pesticides, amino acids, water soluble vitamins, etc.
USP L1 and L60
ge
Pa 4
12
NUCLEODUR PFP
HPLC
Key features:
Hydrophobic phase with alternative selectivity in comparison to
classical C18 modifications
Separation principle based on 4 retention mechanisms (polar
interactions (H bonds), dipole-dipole interactions, - interactions, hydrophobic interactions)
Suitable for LC/MS due to low bleeding characteristics
Recommended application:
Aromatic and unsaturated compounds, phenols, halogenated compounds, isomers, polar compounds like pharmaceuticals, antibiotics; high retention of basic compounds
USP L43
Page
136
info@mn-net.com
P
214 ages
21
8
H
H
WAXplus
Polar phase with improved
cross-linking for reduced column bleeding and better temperature stability
FFAPplus
Polar polyethylene glycol phase with nitro
terephthalic acid modification and innovative
cross-linking
OH
n
Good solvent stability, extended column lifetime, better performance in the MS in comparison to conventional Wax phases
Recommended application:
broad ranges of analyses, e.g., for solvents
and alcohols, suitable for aqueous samples
USP G16
ge
Pa 1
26
www.mn-net.com
(OCH2CH2)m
O2N
O
n
Good solvent stability, extended column lifetime, better performance in the MS in comparison to conventional Wax phases,
suitable for aqueous samples
Recommended application:
FAMEs, allows determination of free carboxylic acids without derivatization
USP G35/close equivalent to G25
Pa
26 ge
3
Chromatography
PTIMA WAXplus
O
OPTIMA FFAPplus
Notes
Chromatography
info@mn-net.com
SPE accessories
High-throughput SPE
2
7
10
50
52
53
MULTI 96 54
CHROMABOND XTR
Phase separation
Flash chromatography
56
58
59
64
CHROMABOND
Flash RS cartridges
Sample preparation
Liquid-liquid extraction
60
Sample clarification
CHROMAFIL syringe filters
66
CHROMAFIL
76
75
www.mn-net.com
77
78
103
Food analysis
ta
il
bl
et
bl
d
Ta
Fruit
oo
ge
So
Ve
Bl
in
Drinking water
Ur
od
Fo
e
st
oi
Pesticides
PAHs PCBs
Drugs
Dyes
Vitamins
before SPE
after SPE
Time [min]
info@mn-net.com
3. Washing 4. Elution
Retention of interfering
components
Moisturizing of the
solid phase with the
matrix
1. Conditioning
2. Sample application
Sample is pressed or
drawn through the
solid phase
Since analytes can be either adsorbed on the SPE packing material or directly flow through while the interfering
substances are retained, two general separation procedures are possible both cases are shown in the figure
above.
www.mn-net.com
mechanisms used in SPE are reversed phase (RP), normal phase (NP) and ion exchange.
Reversed Phase Extraction of hydrophobic or polar organic analytes from aqueous matrix
Normal Phase
Extraction of polar analytes from non-polar organic solvents
Ion Exchange
Extraction of charged analytes from aqueous or non-polar organic samples
Si
Polar interactions
Si
Si
Silica-based:
Other:
Interactions:
Sample:
Elution:
H
O
Cation exchangers
Na
Silica-based:
Polymer-based:
Interaction:
NH3
SO3
Sample:
Elution:
Si
Na
Anion exchangers
Silica-based:
Polymer-based:
Interaction:
OH
Si
NR
OOC
OH
Sample:
Elution:
It should be noted, that in SPE the interactions described above are not found in pure form, but in combination. For
example, modified silicas, unless they have been subjected to endcapping (silanization of residual silanol groups
with short-chain silanes), still possess free silanol groups, which can enter into secondary interactions.
info@mn-net.com
The matrix (sample environment) should be suitable for retention of the analyte.
For solid samples there are different methods to convert the sample into a suitable matrix:
Dissolution of the solid sample in a suitable solvent
Lyophilization of the sample and dissolution in a
suitable solvent
In order to find the suitable solvent, one has to consider all desired sample components. Also, the suitable solvent should enhance retention of the analyte.
For example, samples with large contents of solids are
often homogenized in nonpolar solvents like hexane,
while for samples with high water content dissolution
in acids, bases, buffers or very polar solvents such as
methanol is recommended.
All of our bonded phases and SPE products are vigorously tested for
perfect reproducibility from lot-to-lot and within every single batch
Careful attention to particle size distribution and pore diameters assures consistent column flow
Chemical reproducibility is guaranteed by strict quality control
throughout manufacturing
All products are individually tested to meet our strict quality specifications, ensuring our outstanding product reproducibility, reliability
and performance
Each product is supplied with a certificate of analysis stating the
results of internal examinations and quality control
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CHROMABOND LV
Sample reservoir
polypropylene or glass
Sample reservoir
polypropylene, 15 mL
CHROMABOND MULTI
96
84
72.3
CHROMABOND
90
Adsorbent
Filter
elements
Adsorbent
Adsorbent
Adsorbent
All CHROMABOND columns, cartridges and 96-well plates are manufactured from polypropylene (PP) with
lowest content of extractables (plasticizers, stabilizers, ) offering blank value free results when using most
common solvents. The high quality CHROMABOND adsorbents are kept in place by chemically very inert polyethylene filter elements (PE, standard pore size 20m).
CHROMABOND polypropylene columns
PP columns with PE filter elements
Especially suited for convenient solid phase extraction of small sample volumes
CHROMABOND LV columns
Readily adaptable to all common automated/robotic handling systems (for details see page 54)
Online-SPE (see page 53)
info@mn-net.com
Ordering information
Contents of the kit
CHROMABOND
HR-Xpert development kit I
CHROMABOND
HR-Xpert development kit II
CHROMABOND
polymer development kit
CHROMABOND
standard development kit
columns with 3mL, 60mg each (particle size 45m): 10 columns with
HR-X; 5 columns each with HR-XC, HR-XA, HRXCW, HR-XAW
columns with 3mL, 200mg each (particle size 85m): 10 columns with
HR-X; 5 columns each with HR-XC, HR-XA, HRXCW, HR-XAW
5 columns each with 3mL, 200mg:
HR-X, HR-XC (MCX), HR-XA (MAX), HR-P, Easy, PS-H+, PS-OH
5 columns each with 3mL, 500mg:
C18, C18 ec, C8, C6H5, NH2, DMA, OH, CN, SiOH, SA (SCX), SB (SAX)
REF
730723
730726
730288
730496
730197
730207
731883
730490
730491
731886
730492
730493
731887
CHROMABOND polar development kit I 10 columns each with 3mL, 500mg: SiOH, Florisil, NH2, CN, OH
CHROMABOND polar development kit II 10 columns each with 1mL, 100mg: SiOH, Florisil, NH2, CN, OH
10 cartridges each CHROMAFIX S: SiOH, Florisil, NH2, CN, OH
CHROMAFIX polar development kit
730199
730208
731884
CHROMABOND
ion exchange development kit I
CHROMABOND
ion exchange development kit II
CHROMAFIX
ion exchange development kit I
CHROMABOND
cation exchange development kit I
CHROMAFIX
cation exchange development kit
730206
10 columns each with 3mL, 200mg HR-P, 6mL, 1000mg C18 ec, 6mL,
2000mg C18 PAH, 6mL, 500/1000mg CN/SiOH,
3mL, 500/500mg SA/SiOH
5 columns each with 3mL, 500/500mg SiOH-H2SO4/SA, 3mL, 500mg
SiOH, 6mL, 1000mg Florisil, 3mL, 500/500mg SA/SiOH, 6mL,
700/2000/700mg NAN
www.mn-net.com
Designation
730209
731885
730494
731888
730205
730349
Matrix
Modification/Application
HR-X
Easy
PS/DVB
PS/DVB
polar, bifunctional
HR-P
PS/DVB
PS-RP
C18 ec
PS/DVB
silica
C18 ec f
C18
silica
silica
C18 f
C18 PAH
silica
silica
C18 Hydra
silica
C8
silica
C4
C2
C6H11 ec
C6H5
silica
silica
silica
silica
butyl
dimethyl
cyclohexyl, endcapped
phenyl
Reversed phases
Normal phases
SiOH
silica
unmodified
NH2
silica
aminopropyl
OH (Diol)
CN
silica
silica
diol
cyano
Alox A
Alox N
Alox B
Florisil
PA
polyamide 6
SB
silica
Ion exchangers
Similar phases*
Page
21
22
23
23
24
24
42
25
14
20
info@mn-net.com
26
27
27
28
29
32
31
30
30
33
33
33
34
34
37
Modification/Application
PCA
silica
PSA
HR-XC
silica
PS/DVB
HR-XA
PS/DVB
HR-XCW
PS/DVB
HR-XAW
PS/DVB
PS-OH
PS-H+
PS-Mix
PS-Ag+
PS-Ba2+
PS/DVB
PS/DVB
PS/DVB
PS/DVB
PS/DVB
Dry
Drug
Na2SO4
silica
SA
silica
Similar phases*
silica
www.mn-net.com
35
35
16
17
19
18
22
22
22
22
22
Strata Screen-C Bond Elut Certify I DSCMCAX Clean Screen DAU Accubond Evidex
Bakerbond Narc-2 Isolute HCX LiChrolut
TSC HyperSep Verify CX
Strata Screen-A Bond Elut Certify II Clean
Screen THC Bakerbond Narc-1 Isolute
HAX HyperSep Verify AX
36
Page
Code
47
38
39
40
40
41
44
42
43
45
46
44
47
48
58
56
CHROMABOND HR-Xpert
The professional concept of innovative SPE phases
The CHROMABOND HR-Xpert family comprises 5 polymer-based RP and mixed-mode ion
exchange phases:
CHROMABOND HR-X
hydrophobic PS/DVB copolymer
CHROMABOND HR-XC
strong mixed-mode cation exchanger
CHROMABOND HR-XA
strong mixed-mode anion exchanger
CHROMABOND HR-XCW
weak mixed-mode cation exchanger
CHROMABOND HR-XAW
weak mixed-mode anion exchanger
HR-X
Broad spectrum of application with special suitability for enrichment of pharmaceuticals from biological matrices
onventional
c
PS/DVB copolymer
CHROMABOND HR-X
0
10
20
30
40
50
CHROMABOND HR-Xpert is the perfect combination for all tasks in sample preparation
10
info@mn-net.com
60
min
CHROMABOND HR-Xpert
Chemical structures of the phases:
CHROMABOND HR-X
CHROMABOND
HRXCW
CHROMABOND
HRXA
OH
O
OH
N
CHROMABOND
HRXAW
CHROMABOND
HRXC
SO3
N
H
weak acidic
cation exchanger
Similar phases:
CHROMABOND HR-X:
strong basic
anion exchanger
weak basic
anion exchanger
strong acidic
cation exchanger
Hydrophobic
polystyrene-divinylbenzene copolymer
spherical base material for efficient enrichment
and ideal flow behavior
www.mn-net.com
11
CHROMABOND HR-Xpert
2 particle sizes - 1 goal: HR-Xpert for optimized sample preparation
For different application requirements the particle sizes complement each other perfectly.
Ideal for:
45 m
Recommended for:
+smaller sample
volumes
85 m
+smaller
adsorbent weights
+operation without
vacuum possible (e.g.,
for volatile analytes)
+lower elution
volumes
About half the radius results in 8fold particle number per volume for approx. equal adsorbent weight
Same specific surface for both particle sizes:
considerably larger freely accessible external surface for 45m particles
Method: 1mL column with 30mg CHROMABOND HR-X, 1mL standard solution (1mg/mL hexobarbital), drying, elution in portions of 100L with methanol (see application 305490 at www.mn-net.com/apps)
Recovery rates 45 m
70
Advantages of 45m
particles:
Recovery rates 85 m
70
+faster elution
60
50
40
30
20
10
0
60
50
40
30
20
10
0
Method: 1mL column with 15mg CHROMABOND HR-X, apply portions of 1mL standard solution (250g/mL
hexobarbital in water), collect eluates (see application 305480 at www.mn-net.com)
100
45m (red)
90
The analyte is completely retained up to fraction 10.
20
45m particles provide better enrichment and
breakthrough behavior for small adsorbent weights.
When using larger adsorbent weights this effect is
less pronounced, since then analytes have sufficient
contact with the 85m adsorbent particles as well.
80
Breakthrough [%]
85m (blue)
Small amounts even break through with fraction 4.
Breakthrough [%]
70
15
60
10
50
40
30
20
10
0
10
20
30
40
Number of fractions
50
10
15
Number of fractions
45 m
20
85 m
45m particles are ideal for small sample and elution volumes, while for large amounts of sample and
adsorbent 85m particles show advantages due to better flow properties.
12
info@mn-net.com
CHROMABOND HR-Xpert
The CHROMABOND HR-Xpert concept
for neutral, acidic and basic analytes
3 paths - 1 goal: cleaner samples
Depending on the character of the analytes HR-Xpert offers suitable adsorbents and optimal methods for
sample preparation, cleaning and concentration.
Acids
pKa 28
CHROMABOND
HR-XA
Method A
1.Sample
pretreatment
2.Column
conditioning
3.Sample
application
4.Washing1:
(basic)
aqueous
5.Washing2/
Elution 1*:
organic
6.Elution 2: organic acidic
Strong
bases
Acidic
compounds
Neutral
compounds
CHROMABOND
HR-X
Strong acids
pKa < 1
CHROMABOND
HR-XAW
Method B
Method C
1.Sample
pretreatment
2.Column
conditioning
3.Sample
application
4.Washing:
aqueous
1.Sample
pretreatment
2.Column
conditioning
3.Sample
application
4.Washing1:
(acidic)
aqueous
5.Washing2/
Elution 1*:
organic
6.Elution 2:
organic basic
5.Elution:
organic
Neutral
compounds*
Bases
pKa 210
CHROMABOND
HR-XC
Strong
acids
Strong bases
pKa > 10
CHROMABOND
HR-XCW
Basic
compounds
* Under organic washing and elution conditions the following compounds will be also eluted:
HR-X:
HR-XA, HR-XAW:
HR-XC, HR-XCW:
www.mn-net.com
13
Excellent recovery rates especially for the enrichment of pharmaceuticals and active ingredients due to the spherical structure of the
particles, very homogeneous surface, and optimized pore structure
Column type:
CHROMABOND HR-X, 3mL, 200mg
REF 730931
Sample: 1g/mL each in water
Column conditioning: 5mL methanol, 5mL dist. water
Sample application: slowly aspirate 500mL water (pH 3) through
the column
Column washing: 5mL water
Elution: after drying 3 x 2mL acetonitrile
Further analysis: HPLC on NUCLEODUR C18 Gravity, 5m;
see MN Appl. No. 121690
Pharmaceuticals/active
ingredients from tablets,
creams and water/waste
water
Column type:
CHROMABOND HR-X, 3mL, 200mg
REF 730931
Sample pretreatment: samples are spiked with 500ng of each
pesticide in 1000mL water, adjusted to pH 2 with HCl or pH 7
Column conditioning: 10mL methanol, 10mL dist. water
Sample application:
slowly pass 1000mL spiked water sample through the column
with the aid of a tubing adapter (REF 730243)
Elution: after drying 5mL methanol THF (1:1, v/v)
Further analysis: HPLC
Recovery rates [%]
Recommended application:
HR-X
98
91
99
92
63
53
Strata X
92
93
95
93
45
39
MN Appl. No. 304240
Compound
Metamitron
Quinmerac
Chloridazon
Picloram
Metribuzin
Cyanazine
Metabenzthiazuron
Chlortoluron
Isoproturon
Diuron
Dimethenamid-P
Linuron
Epoxyconazole
Penconazole
Alachlor
Propiconazole-1
Flufenacet
Diflufenicam
Triallate
HR-X
pH 2
86
90
93
83
84
83
94
91
89
91
89
94
85
90
93
89
91
58
42
Compound
Desisopropylatrazine
2,4-Dichlorobenzamide
Desethylatrazine
Hexazinone
Bromacil
Simazine
Desethylterbuthylazine
Atrazine
Metalaxyl
Metazachlor
Propazine
Terbuthylazine
Metolachlor
HR-X
pH 7
90
95
89
95
103
91
89
88
97
93
88
86
97
14
info@mn-net.com
Column type:
CHROMABOND HR-X, 3mL, 200mg
REF 730931
Sample pretreatment: if necessary, adjust pH value
Column conditioning: 5mL methanol
Equilibration: 5mL water
Sample application:
slowly aspirate the sample through the column
Column washing: 5mL water methanol (95:5, v/v)
Elution: after drying 3 x 2mL methanol
Compounds:
100
A phenobarbital
B pentobarbital
C hexobarbital
50
A B C
A B C
A B C
Batch 1
Batch 2
Batch 3
Ordering information
Volume
Adsorbent weight
Pack of
30mg
730934
60mg
100mg
730935
730936
200mg
500mg
1g
730931
730938
730937
730939
730940
730941
3mL
6mL
500mg
730939.250
30mg
730934P45
60mg
730936P45
100mg
730935P45
30mg
732130
60mg
732131
96 x 25mg
(45m)
738530.025M
www.mn-net.com
200mg
30
30
30
20
250
250
730931P45
30
30
200mg
732132
30
96 x 50mg
(85m)
738530.050M
96 x 100mg
(85m)
738530.100M
730663
Column type:
CHROMABOND HR-X, 3mL, 200mg
REF 730931
Sample: 100ng/mL each in serum
Column conditioning: 5mL methanol, 5mL dist. water
Sample application: 1mL spiked serum
Column washing: 5mL water
Elution: after drying 3 x 2mL methanol
1
20g
15
Recommended application:
Column type:
CHROMABOND HR-XC, 3mL, 200mg
REF 730952
Sample pretreatment: individual sample preparation with reference to analytes and matrix
Column conditioning: 5mL methanol
Equilibration: 5mL water
Sample application: slowly aspirate sample through the column
Washing 1: 2mL 0.1mol/L HCl in water
Washing 2/Elution 1: 2mL methanol (neutral and acidic compounds); if necessary, further washing steps
Elution 2: after drying 5mL methanol 5% NH3 (basic compounds)
Further analysis: if necessary, evaporate and redissolve in a
suitable solvent; HPLC or GC
MN Appl. No. 304740
Column type:
CHROMABOND HR-XC, 3mL, 200mg
REF 730952
Sample: 1mL spiked matrix, acidified with 200L 2% H3PO4
Column conditioning: 5mL methanol, then 5mL water
Sample application: slowly aspirate sample through the column
Washing: 2mL 0.1mol/L HCl
Elution: 2.5mL methanol (fraction A: neutral and acidic analytes); then 5mL methanol NH3 90:10, v/v (fraction B: basic
analytes)
Further analysis for fraction A: HPLC, e.g., on NUCLEODUR
C18 Gravity, see MN Appl. No. 122230; for fraction B: HPLC on
NUCLEODUR C8 Gravity, see MN Appl. No. 118520
Recovery rates [%]
Fraction A:
neutral and acidic
analytes
Compound
HR-XC
Suprofen
Naproxen
Tolmetin
Phenobarbital
Indomethacin
Hexobarbital
108
85
73
108
33
80
Fraction B:
basic analytes
HR-XC Oasis Strata
MCX
X-C
1. Doxepin
101
68
82
2. Imipramine
95
71
85
3. Amitriptyline
94
72
78
4. Trimipramine 92
70
81
Compound
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
30mg
730969
Adsorbent weight
730956
100mg
730049
150mg
730957
1mL 730969P45
3mL
730956P45
730049P45
CHROMABOND
S
155mg
731755
Pack of
200mg
500mg
730952
730953
730955
16
30
30
730952P45
M
240mg
731756
30
30
30
L
500mg
731757
30
730664
100g
info@mn-net.com
serum sample
without SPE
2.000
1.000
60
40
Weak/medium-strength
acids with pKa 28
Column type:
CHROMABOND HR-XA, 3mL, 200mg
REF 730951
Sample pretreatment: individual sample preparation with reference to analytes and matrix
Conditioning: 5mL methanol
Equilibration: 5mL water
Sample application: slowly aspirate sample through the column
Washing 1: 2mL 0.1mol/L NaOH in water
Washing 2/Elution 1: 2mL methanol (neutral and basic compounds), if necessary, further washing steps
Elution 2: after drying 5mL methanol 1 to 10% formic acid
(acidic compounds)
20
Phenolic acids,
acidic herbicides
10
15
www.mn-net.com/apps
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
30mg
730968
Adsorbent weight
730950
100mg
730727
150mg
730958
1mL 730968P45
3mL
730950P45
730727P45
CHROMABOND
www.mn-net.com
S
155mg
731768
Pack of
200mg
500mg
730951
730954
730966
30
30
30
30
30
730951P45
M
240mg
731769
3.000
100
80
Recommended application:
3.500
mAU
L
500mg
731770
50
730671
100g
17
Column type:
CHROMABOND HR-XCW, 3mL, 200mg
REF 730739
Sample pretreatment: individual sample preparation with reference to analytes and matrix
Column conditioning: 5mL methanol
Equilibration: 5mL acidified water
Sample application: slowly aspirate sample through the column
Washing 1: 2mL acidified water
Washing 2/Elution 1: 2mL methanol (neutral and acidic compounds), if necessary, further washing steps
Elution 2: after drying 2 x 2mL methanol 1 to 5% formic acid
(strongly basic compounds)
Recommended application:
Analysis of perfluorinated
Column:
Eluent:
2
6
10
min
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
Adsorbent weight
30mg
730731
CHROMABOND
60mg
730735
100mg
730733
150mg
730737
Pack of
200mg
500mg
730739
730741
730743
1mL 730731P45
3mL
730735P45
730733P45
S
155mg
731774
M
240mg
731775
30
30
30
30
30
730739P45
18
L
500mg
731776
50
730674
100g
info@mn-net.com
Recommended application:
Perfluorinated surfactants
Acidic compounds like sulfonates
Active ingredients from
heavily matrix-contaminated samples like, e.g., urine,
plasma, serum
Strong acids with pKa<1
Recovery
103
94
94
95
81
82
MN Appl. No. 305140
Column type:
CHROMABOND HR-XAW, 3mL, 200mg
REF 730748
Sample pretreatment: individual sample preparation with reference to analytes and matrix
Conditioning: 5mL methanol
Equilibration: 5mL water
Sample application: slowly aspirate sample through the column
Washing 1: 25mmol/L ammonium acetate
Washing 2/Elution 1: 2mL methanol (neutral and basic compounds), if necessary, further washing steps
Elution 2: after drying 2 x 2mL methanol 1 to 5% ammonia
(strongly acidic compounds)
Further analyses: if necessary, evaporate and redissolve in a
suitable solvent; HPLC or GC
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
30mg
730728
CHROMABOND
Adsorbent weight
730747
100mg
730729
730749
730747P45
730729P45
CHROMABOND
www.mn-net.com
150mg
200mg
500mg
730748
730744
730745
1mL 730728P45
3mL
Size
adsorbent weight
Pack of
S
155mg
731771
730748P45
M
240mg
731772
30
30
30
30
30
L
500mg
731773
50
730673
100g
19
Without preconditioning
Recommended application:
Recovery of pesticides
Recovery
90
93
93
69
65
81
93
91
94
91
92
Compound
Metalaxyl
Isoproturon
Diuron
Metazachlor
Propazine
Terbuthylazine
Linuron
Metolachlor
Triallate
Standard
Recovery
96
94
94
97
95
93
96
97
61
64
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
15mL
30mg
730751
CHROMABOND
Adsorbent weight
730753
200mg
500mg
1g
730754
730755
730759
730756
730757
730758
3mL
6mL
CHROMABOND LV-Easy
15mL
CHROMABOND
100mg
730752
Pack of
Easy adsorbent
200mg
730754.250
730755.250
250
250
200mg
732472
30
96 x 50mg
738520.050M
96 x 100mg
738520.100M
730661
20g
20
30
30
30
20
info@mn-net.com
Recommended application:
aromatic compounds
phenols from water
nitroaromatics from water
pesticides from water
PAHs from oil
Private communication M. Le, T.C. Schmidt, Department of Chemistry, University Marburg, 1997
For recovery rates of numerous aromatic amines please see application 301810 at www.mn-net.com/apps.
Ordering information
Volume
Adsorbent weight
CHROMABOND
3mL
CHROMABOND
100mg
730280
200mg
500mg
1g
730108
730119
730117
730111
730118
730108.250
CHROMABOND LV-HR-P
S
200mg
731839
M
330mg
731840
30
30
30
250
500mg
1g
730111G
730118G
200mg
732108
15mL
Size
Adsorbent weight
200mg
730108G
3mL
6mL
www.mn-net.com
Pack of
30
30
Sample application:
aspirate sample through the column with about 10mL/min
Column washing:
wash with 2mL dist. water, dry 5min under vacuum
Elution: 3 x 1mL methanol acetonitrile (1:1, v/v)
30
L
680mg
731841
96 x 100mg
50
738111.100M
730615
20g
21
Recommended application:
PS-RP
PS-OH
PS-H+
PS-Mix
PS-Ag+
PS-Ba2+
Application 301930/302750: removal of halides from aqueous samples shown for the trace analysis of
nitrate besides an excess of chloride or bromide
Compounds investigated: 20ppm nitrate besides
2500ppm chloride or 500ppm bromide, respectively
Column type:
CHROMAFIX PS-Ag+ (M)
0.8mL, 480mg, REF 731865
Column conditioning: 1mL dist. water
Ordering information
Phase
CHROMABOND
PS-RP
PS-OH
PS-H+
PS-Mix
PS-RP
PS-OH
PS-H+
PS-Mix
PS-Ag+
PS-Ba2+
PS polypropylene columns
3mL
200mg
730765
730396
730690
CHROMAFIX
22
3mL
500mg
730692
730344
730376
730394
PS cartridges
Size
S
731877
731868
731867
731909
731866
731871
Adsorbent
weight
200mg
200mg
230mg
230mg
240mg
280mg
6mL
500mg
730693
730378
730377
Size
M
731875
731860
731861
Adsorbent
weight
320mg
380mg
430mg
731865
731870
480mg
550mg
Pack of
6mL
900mg
30
30
30
30
730310
Size
L
Adsorbent
weight
731862
731863
800mg
900mg
info@mn-net.com
50
50
50
50
50
50
Recommended application:
nonpolar compounds
aflatoxins, amphetamines, antibiotics,
antiepileptics, barbiturates, caffeine, drugs,
preservatives, fatty acids, nicotine, PAHs,
pesticides, PCBs, heavymetals, vitamins
very well suited for desalting of samples
C18 ec f for viscous samples
Ordering information
Volume
1mL
3mL
6mL
15mL
45mL
70mL
100mg
730011
Adsorbent weight
Pack of
500mg
1g
2g
730012
730013
730014
730015
730141
730404
3mL
6mL
200mg
730012G
CHROMABOND LV-C18 ec
15mL
200mg
732012
500mg
730013G
730014G
CHROMABOND
10g
730405
730259
1g
100
50
30
20
20
10
250
250
730015.250
1g
50
30
730015G
500mg
732013
30
5g
S
270mg
731804
M
530mg
731805
L
950mg
731806
50
96 x 25mg
738011.025M
96 x 50mg
738011.050M
96 x 100mg
738011.100M
730611
100g
MULTI 96 C18 ec
CHROMABOND
200mg
730269
500mg
730018
730016
1g
www.mn-net.com
50
30
730010
730613
100g
23
octadecyl silica
Base material silica, pore size 60, particle size 45m for C18,
100m for C18f (for fast flow), specific surface 500m2/g,
pH stability 28
Recommended application:
nonpolar compounds
pesticides
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
15mL
45mL
70mL
100mg
730001
CHROMABOND
Adsorbent weight
Pack of
200mg
500mg
1g
2g
730002
730003
730004
730005
730130
730028
5g
10g
730400
730261
3mL
6mL
500mg
730003.250
730004.250
3mL
6mL
500mg
730003G
730004G
1g
250
250
730005.250
1g
50
30
730005G
CHROMABOND LV-C18
15mL
200mg
732002
30
CHROMABOND
CHROMABOND
100
50
30
20
20
10
S
240mg
731801
M
480mg
731802
MULTI 96 C18
96 x 25mg
738001.025M
C18 adsorbent
L
950mg
731803
50
96 x 100mg
738001.100M
730602
100g
200mg
730402
500mg
730008
730403
1g
24
50
30
730009
730612
info@mn-net.com
100g
Special octadecyl phase for polar analytes, not endcapped, carbon content 15%
Recommended application:
Ordering information
Volume
Adsorbent weight
Pack of
50mg
730294
CHROMABOND
3mL
6mL
100mg
730295
500mg
1g
2g
3g
730296
730297
730299
730298
730300
730301
730302
730296G
200mg
CHROMABOND
S
270mg
731730
www.mn-net.com
1g
730298G
730300G
50
30
200mg
732295
500mg
730297G
730299G
100
50
30
30
M
530mg
731731
L
950mg
731732
96 x 100mg
50
738294.100M
730628
100g
25
octyl silica
Recommended application:
pesticides, PCB
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
CHROMABOND
6mL
CHROMABOND
Adsorbent weight
C8 polypropylene columns
100mg
730021
C8 glass columns
LV-C8
15mL
CHROMAFIX C8 cartridges
Size
Adsorbent weight
CHROMABOND MULTI 96 C8
CHROMABOND C8 adsorbent
Pack of
200mg
500mg
1g
730022
730023
730024
730134
100
50
30
500mg
730024G
30
500mg
732023
30
520mg
731808
50
96 x 100mg
738021.100M
730601
100g
info@mn-net.com
butyl silica
Recommended application:
Ordering information
Volume
CHROMABOND
Adsorbent weight
C4 polypropylene columns
CHROMAFIX C4 cartridges
Size
Adsorbent weight
S
220mg
731740
500mg
730227
100
50
M
440mg
731741
50
CHROMABOND C4 adsorbent
730651
100g
C2
dimethyl silica
Recommended application:
Similar to C4
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
CHROMABOND
Adsorbent weight
C2 polypropylene columns
100mg
730169
Pack of
500mg
1g
730221
730409
730410
C2 adsorbent
730652
100mg
730225
1mL
3mL
Pack of
100
50
30
100g
www.mn-net.com
27
Recommended application:
phenols from water
chloroanilines from waste
water
anthelmintics from tissue
Compounds investigated:
phenol, 2,4-dinitrophenol, pentachlorophenol
Column types:
CHROMABOND C18, 6mL, 2000mg, REF 730130
CHROMABOND C6H11 ec, 6mL, 2000mg, REF 730469
Column conditioning:
10mL acetone, 10mL methanol, and 10mL dist. water (pH 2)
Sample application:
aspirate the sample through the column.
Elution:
10mL methanol
100
80
60
40
20
0
phenol
2,4-dinitrophenol
pentachlorophenol
C18
C6H11 ec
MN Appl. No. 302150
Ordering information
Volume
Adsorbent weight
500mg
730442
730443
Pack of
1g
50
30
730444
730631
www.mn-net.com/apps
28
info@mn-net.com
100g
phenyl silica
Base material silica, pore size 60, particle size 45m, specific
surface 500m2/g, pH stability 28
Phenyl phase, carbon content 8%
Polarity similar to C8
Recommended application:
aflatoxins
caffeine
phenols
Ordering information
Volume
Adsorbent weight
100mg
730083
200mg
500mg
730411
730084
Pack of
100
50
730606
100g
www.mn-net.com
29
cyanopropyl silica
Recommended application:
cyclosporins
carbohydrates
Ordering information
Volume
Adsorbent weight
1mL
3mL
6mL
100mg
730061
200mg
500mg
730420
730063
730421
CHROMABOND CN adsorbent
Pack of
100
50
30
730607
100g
OH (Diol)
diol silica
Recommended application:
antibiotics
prostaglandins
Polar
Ordering information
Volume
Adsorbent weight
Pack of
100mg
730051
200mg
500mg
730417
730053
730418
100
50
30
730605
30
info@mn-net.com
100g
aminopropyl silica
Recommended application:
trace elements
lipids
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
CHROMABOND
3mL
CHROMABOND
Adsorbent weight
200mg
500mg
1g
730413
730033
730180
730626
3mL
6mL
CHROMABOND LV-NH2
Size
Adsorbent weight
CHROMABOND
CHROMABOND
www.mn-net.com
MULTI 96 NH2
NH2 adsorbent
500mg
730033.250
500mg
730033G
730180G
100
50
30
250
1g
730626G
500mg
732033
15mL
Pack of
50
30
30
S
220mg
731813
50
96 x 100mg
738031.100M
730603
100g
31
unmodified silica
Unmodified, weakly acidic silica, pore size 60, particle size 45m,
specific surface 500m2/g, pH stability 28
Very polar
Adsorbs humidity from air, for this reason it should be kept well
closed and if necessary dried before use
Due to its high affinity for polar compounds it should not be conditioned with polar (e.g., methanol) or water-containing solvents.
Recommended application:
aflatoxins
chloramphenicol
pesticides
steroids
vitamins
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
15mL
45mL
70mL
150mL
100mg
730071
CHROMABOND
1g
2g
730214
730073
730070
730075
730107
730217
500mg
730073.250
200mg
730214G
CHROMABOND LV-SiOH
15mL
200mg
732072
500mg
730073G
730070G
CHROMABOND
730406
10g
730072
50g
730473
1g
2g
730075.250
730107.250
1g
2g
730075G
730107G
S
230mg
731828
MULTI 96 SiOH
SiOH adsorbent
100
50
30
20
20
10
10
250
250
50
30
500mg
732073
5g
Pack of
200mg
3mL
6mL
CHROMABOND
32
Adsorbent weight
30
M
420mg
731829
L
880mg
731830
50
96 x 100mg
738071.100M
730608
100g
info@mn-net.com
Aluminium oxide, high purity, pore volume 0.90mL/g, particle size 60150m, specific surface 150m2/g
pH value 4 0.5
pH value 7 0.5
pH value 9.5 0.5
Ordering information
Phase
Volume
CHROMABOND
A
A
A
N
N
N
B
B
B
3mL
6mL
45mL
3mL
6mL
45mL
3mL
6mL
45mL
Alox N
Alox B
6mL
6mL
Alox A
Alox N
Alox B
15mL
15mL
15mL
CHROMABOND
500mg
730452
730453
730017
730446
730447
730139
730429
730466
730020
CHROMABOND LV-Alox
Size
Adsorb. weight
CHROMABOND
Alox A
Alox N
Alox B
MULTI 96 Alox
www.mn-net.com
1g
Pack of
4g
730455
730250
730467
50
30
20
50
30
20
50
30
20
1g
730139G
730020G
30
30
1g
732210
732091
732205
30
30
30
M
850mg
731844
L
1700mg
731845
96 x 100mg
738253.100M
738251.100M
738252.100M
50
Alox
Alox
Alox
Alox
Alox
Alox
Alox
Alox
Alox
Adsorbent weight
1
1
1
730642
730641
730640
100g
100g
100g
33
magnesium silicate
Ordering information
Volume
CHROMABOND
Florisil
Recommended application:
organic tin compounds,
aliphatic carboxylic acids,
PCBs, PAHs
Adsorbent weight
Pack of
polypropylene columns
200mg
730457
3mL
6mL
500mg
730081
730238
1g
2g
730082
730239
CHROMABOND
1g
730082.250
Florisil
glass columns
6mL
500mg
730238G
1g
730082G
CHROMABOND
Florisil
adsorbent
250
2g
730239G
30
L
990mg
731848
50
730622
100g
PA
polyamide 6
Ordering information
Volume
CHROMABOND
PA polypropylene columns
200mg
730384
CHROMAFIX PA cartridges
Size
Adsorbent weight
CHROMABOND
Recommended application:
flavonoids, PAHs
Adsorbent weight
3mL
6mL
S
170mg
731849
PA adsorbent
500mg
730126
730007
Pack of
1g
730127
50
30
L
620mg
731851
50
730660
34
50
30
info@mn-net.com
100g
Recommended application:
strong cations
Ordering information
Volume
Adsorbent weight
500mg
730482
730483
15mL
500mg
732482
CHROMABOND LV-PCA
1g
730484
30
730629
PSA
50
30
100g
Recommended application:
weak cations
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
CHROMABOND
Glass columns, LV columns,
Adsorbent weight
730462
PSA adsorbent
CHROMAFIX
www.mn-net.com
500mg
Pack of
1g
730464
730630
3mL
6mL
Pack of
100
50
30
100g
35
Recommended application:
amino acids
amines
chlorophyll
PCB
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
CHROMABOND
3mL
CHROMABOND
Adsorbent weight
SA polypropylene columns
100mg
730076
200mg
500mg
1g
730275
730077
730425
730212
15mL
CHROMAFIX SA cartridges
Size
Adsorbent weight
CHROMABOND
CHROMABOND
S
220mg
731831
MULTI 96 SA
SA adsorbent
Pack of
100
50
30
500mg
730077.250
250
500mg
732083
30
M
450mg
731832
L
920mg
731833
50
96 x 100mg
738141.100M
730609
100g
36
info@mn-net.com
Recommended application:
organic acids
caffeine
saccharin
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
CHROMABOND
3mL
CHROMABOND
Adsorbent weight
SB polypropylene columns
100mg
730078
200mg
500mg
1g
730322
730079
730426
730323
15mL
CHROMAFIX SB cartridges
Size
Adsorbent weight
CHROMABOND
CHROMABOND
Glass columns on request
www.mn-net.com
Pack of
S
230mg
731834
MULTI 96 SB
SB adsorbent
100
50
30
500mg
730079.250
250
500mg
732088
30
M
460mg
731835
L
920mg
731836
50
96 x 100mg
738101.100M
730610
100g
Column type:
CHROMABOND SB ( SAX), 3mL, 500mg
REF 730079
Sample pretreatment:
homogenize 10g food sample in 100mL 0.01mol/L phosphate buffer pH7.4 and filter
Column conditioning: 2 column volumes n-hexane, then 2 column
volumes methanol, finally 2 column volumes dist. water
Sample application: force or aspirate 10mL of the filtrate through
the column
Column washing: 2 column volumes dist. water
Elution: 5mL 10% sodium chloride in 0.1mol/L sodium acetate
buffer
37
Base material silica, pore size 60, particle size 45m, specific surface 500m2/g, pH stability 28
Recommended application:
Ordering information
Volume
Adsorbent weight
100mg
730681
200mg
500mg
730168
730684
730682
Pack of
100
50
30
CHROMABOND LV-Drug
15mL
38
200mg
730168.250
250
200mg
732168
30
96 x 100mg
738161.100M
info@mn-net.com
Recommended application:
Ordering information
Volume
CHROMABOND
1mL
3mL
6mL
Adsorbent weight
CHROMABOND LV-Drug II
15mL
www.mn-net.com
Pack of
200mg
500mg
730680
730686
730683
Compounds investigated:
tetrahydrocannabinol, 11-nor-9-THC-carboxylic acid
Column type:
CHROMABOND Drug II, 3mL, 200mg
REF 730680
Sample pretreatment: add 300L 10mol/L potassium hydroxide solution and internal standard (for GC/MS deuterium labeled
11-nor-9-THC-carboxylic acid) to 5mL urine. Vortex the sample
and then hydrolyze at 60C for 15min. Cool sample and add
200L glacial acetic acid and 2mL 50mmol/L ammonium acetate solution. If necessary, adjust sample pH to 67.
Column conditioning: 2mL methanol, 2mL dist. water; equilibrate column with 2mL 50mmol/L ammonium acetate buffer
Sample application: slowly force or aspirate the sample through
the column (12mL/min)
Column washing: elute interferants with 10mL methanol water
(1:1, v/v); dry the column for 10min at high vacuum; further
wash the column with 2mL acetonitrile and dry for another 2min
Elution: elute THC metabolites with 3mL hexane ethyl acetate
glacial acetic acid (75:25:1, v/v/v)
100
50
30
200mg
732681
30
96 x 100mg
738680.100M
39
Recommended application:
collagen crosslinks in urine
Pyridinoline and deoxypyridinoline are collagen crosslinks occurring in bones and cartilage. If these substances are released, they can be detected in the urine. In cases of increased bone catabolism (e.g., during
osteoporosis) the urine concentrations of pyridinoline and deoxypyridinoline are increased.
Column conditioning:
5mL of the wash solution
Sample application:
force or aspirate the pre-treated sample through the column.
Discard the flow-through. Wash with 1525mL of the wash solution.
Elution:
force or aspirate 35mL dist. water through the column
Ordering information
Volume
CHROMABOND
3mL
Adsorbent weight
Pack of
50
Tetracycline
Recommended application:
Private communication of Mr. Lippold, Chemisches Landesuntersuchungsamt (Chem. Research Agency) Freiburg, Germany
Column washing:
Compounds investigated:
2mL dist. water (removal of Cu ions), 2mL n-hexane
tetracycline, oxytetracycline, chlorotetracycline (100500mg/kg)
Column type:
Elution: 7.5mL methanol into a 25-mL tapered flask. Add 1mL of
CHROMABOND Tetracycline, 6mL, 500mg
an ethylene glycol methanol mixture (22g ethylene glycol filled
REF 730315
up to 100mL with methanol) and evaporate to dryness with a rotaSample pretreatment:
tion evaporator (max. 40C). Fill up the residue to 400mL with
see detailed description in appl. 302030 at
0.1mol/L McIlvain-EDTA buffer (52.5g citric acid H2O, 44.5g
www.mn-net.com/apps
Na2HPO4 H2O and 93g Titriplex III dissolved in 2.5L dist. water,
Column conditioning:
adjusted to pH4 with NaOH).
1 column volume methanol, 1 column volume dist. water, then
Further analysis:
1column volume EDTA succinate buffer
HPLC with column 250 x 4mm NUCLEOSIL 100-5 C18 HD (apCAUTION: DO NOT LET THE COLUMN RUN DRY!
plication 110710)
Sample application:
Recovery rates: tetracycline, chlorotetracycline ~ 5070%,
force or aspirate 50mL of the eluate from the sample pretreatment
oxytetracycline ~ 6080%
through the CHROMABOND column
40
info@mn-net.com
Adsorbent weight
Pack of
HR-P-AOX
500mg
730315
30
AOX from waters with high salt loads (DIN 38409 H22)
Column type:
CHROMABOND HP-P-AOX, 6mL, 500mg
REF 730111.AOX
Column conditioning:
5mL methanol, 10mL dist. water.
Do not let the column run dry!
Sample application:
force or aspirate 100mL original or diluted sample (pH 1)
through the column (35mL/min).
Do not let the column run dry!
Column washing:
50mL nitrate rinsing solution (dissolve 17g NaNO3 in 100mL
dist. water, add 1.4mL HNO3 10mol/L, fill up to 1000mL; take
50mL and fill to 1000mL with dist. water). Discard the flowthrough.
Elution:
slowly aspirate 1 x 1mL, then 1 x 4mL methanol and 10mL dist.
water through the column.
Collect eluates in 100mL volumetric flask and fill to 100mL with
dist. water.
Ordering information
Volume
CHROMABOND
6mL
www.mn-net.com
Adsorbent weight
Pack of
500mg
730111.AOX
Recommended application:
extraction of AOX (adsorbable organically bonded halogens) from waters
containing high salt loads or organic pollutants in accordance with
DIN38409 H22
30
41
Recommended application:
PAHs from water
Column type:
CHROMABOND C18 PAH, 6mL, 2g
REF 730166
Sample pretreatment:
mix 1000mL water sample with 10mL methanol
Column conditioning:
1 column volume methanol, then 1 column volume dist. water
Sample application: aspirate 1000mL water sample through the
column (~ 1520mL/min), then dry column (stream of nitrogen or
24h in a desiccator over P2O5)
Ordering information
Volume
CHROMABOND
6mL
CHROMABOND
6mL
Adsorbent weight
Pack of
2g
730166
30
730166G
30
730616
100g
Recommended application:
PAHs from water containing
humic acids
Column type:
CHROMABOND NH2/C18, 6mL, 500mg/1g glass column
REF 730620G
Sample pretreatment:
mix 500mL water sample with 25mL 2-propanol
Column conditioning: 10mL dichloromethane, 10mL methanol,
then 10mL dist. water 2-propanol (9:1, v/v)
42
Elution: elute with 4mL acetonitrile toluene (3:1, v/v) and then
evaporate or fill up to the volume required
Recovery rates (50ng/L per component): naphthalene 87%,
acenaphthylene 89%, acenaphthene 90%, fluorene 82%,
phenanthrene 85%, anthracene 90%, fluoranthene 89%,
pyrene 89%, benz[a]anthracene 87%, chrysene 95%, benzo[b]
fluoranthene 91%, benzo[k]fluoranthene 89%, benzo[a]pyrene
90%, dibenz[ah]anthracene 97%, benzo[ghi]perylene 91%,
indeno[1,2,3-cd]pyrene 96%
info@mn-net.com
CHROMABOND
Adsorbent weight
Pack of
6mL
500/500mg
730618
500mg/1g
730620
30
6mL
730618G
730620G
30
Na2SO4/Florisil
Recommended application:
Column type:
CHROMABOND Na2SO4/Florisil, 6mL, 2g/2g,
glass column, REF 730249G
Internal standard solution:
dissolve 20mg n-tetracontane (C40H82) in petroleum ether, add
20mL n-decane (C10H22) and fill up to one liter with petroleum
ether. For preparation of the extraction solution dilute standard
solution 1:10 with petroleum ether.
Sample pretreatment:
adjust 900mL water (10C) with HCl (12mol/L) to pH 2 and
add 80g MgSO4. Add 50mL of the extraction solution, close the
bottle and stir the suspension intensely for 30min. Add enough
dist. water to separate the organic from the aqueous phase.
Column conditioning: 5mL petroleum ether
Sample application:
slowly aspirate or force the sample through the column
Elution:
wash with 10mL petroleum ether. Evaporate the combined solution from sample application and elution to 1mL at about 75C.
If necessary, fill up to 1mL again. (If the hydrocarbon content is
high, evaporation to 1mL may not be necessary.)
Recovery rates: must be > 80% for n-tetracontane.
Ordering information
Volume
CHROMABOND
6mL
CHROMABOND
6mL
CHROMABOND
6mL
www.mn-net.com
Na2SO4/Florisil
Na2SO4/Florisil
Na2SO4/Florisil
Adsorbent weight
polypropylene columns
glass columns
Pack of
2g/2g
730249
30
2g/2g
730249G
30
2g/2g
730249G.250
250
43
Recommended application:
Column type:
CHROMABOND CN/SiOH, 6mL, 500/1000mg
REF 730135
Sample pretreatment:
dry 30g soil with sodium sulfate and reflux 4h with 250mL
petroleum ether in a Soxhlet extractor. For low PAH contents
(colorless or weakly colored extracts) concentrate extract to 1/10
of its volume in a rotation evaporator.
Column conditioning:
4mL petroleum ether
Sample application:
aspirate 20mL of the extract through the column
Column washing: 2mL petroleum ether
Elution:
2 x 2mL acetonitrile toluene (3:1, v/v), then evaporate or fill to
the volume required
Further analysis: HPLC, e.g., with column 100 x 4mm
NUCLEODUR C18 PAH, 3m, REF 760783.40 according to
application 123820 (see page 168)
For recovery rates see application 301310 at www.mn-net.com
Ordering information
Volume
Adsorbent weight
Pack of
3mL
6mL
500mg/1g
730112
730135
50
30
6mL
500mg/1g
730135.250
CHROMABOND
6mL
NAN
44
250
500mg/1g
730135G
30
Recommended application
extraction of PCB from
sludge
info@mn-net.com
Ordering information
Volume
CHROMABOND
Adsorbent weight
700/2000/700mg
730149
50
30
6mL
700/2000/700mg
730149.250
250
6mL
700/2000/700mg
730149G
30
730619*
100g
NAN adsorbent
* This product contains harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.
SA/SiOH
SA: strongly acidic cation exchanger based on silica with benzenesulfonic acid modification
SiOH: unmodified silica for removal of polar compounds
Recommended application:
3mL
6mL
Pack of
Ordering information
Volume
CHROMABOND
Adsorbent weight
3mL
6mL
500/500mg
730132
730235
3mL
500/500mg
730132.250
www.mn-net.com
Pack of
50
50
250
45
Recommended application:
Ordering information
Volume
CHROMABOND
3mL
CHROMABOND
3mL
CHROMABOND
3mL
Adsorbent weight
Pack of
50
250
500/500mg
730085G
Kombi-Kit for extraction of PCB from oil with reference to DIN 51527, part 1
25 columns each of CHROMABOND SiOH-H2SO4/SA and CHROMABOND
SiOH
46
730125
info@mn-net.com
50
1 kit
Recommended application:
Ordering information
Adsorbent weight
S
780mg
731852
M
1500mg
731853
L
2800mg
731854
50
Recommended application:
Important notes:
ABC18
Pack of
Ordering information
Volume
Adsorbent weight
www.mn-net.com
Pack of
500mg
730533
30
47
Removes polar compounds (e.g., organic acids, pigments, sugars) from matrices like fruit or vegetables
Similar phases: Supelclean PSA, Bond Elut PSA
10g sample are homogenized with 10mL acetonitrile. After adding the internal standard the sample is shaken with 4g MgSO4
and 1g NaCl and afterwards centrifuged.
1mL of the supernatant is spiked with 25mg CHROMABOND
Diamino and 150mg MgSO4 and shaken again. After centrifugation the supernatant is injected into GC/MS.
For optimizing the extraction of pH-dependent compounds, for minimizing decomposition of sensitive
substances, and for broadening the matrix spectrum,
different modifications of the QuEChERS method have
been elaborated.
48
Recommended application:
info@mn-net.com
Volume Description
Composition
15mL* Mix I
15mL* Mix II
15mL* Mix IV
15mL* Mix V
15mL* Mix VI
CHROMABOND
Pack of
730970
50
730971
50
730972
50
730973
50
730975
50
730974
50
730561
730562
50
30
730653.20
730653
20g
100g
730223
50
QuEChERS mixes
Sample property
Citrate extraction
www.mn-net.com
Diamino/Carbon clean-up
Mix IV
Mix I
Diamino/Carbon clean-up
(higher Carbon content)
Mix V
Citrate extraction
Mix I
REF
Diamino/C18 ec clean-up
Mix VI
49
2 Polypropylene lid
Ordering information
Description
Pack of
REF
consists of glass cabinet with lid and lid gasket, removable needles on lower side of lid, vacuum gauge, control
valve, valves and caps, variable rack:
for up to 12 columns or cartridges (including PP tank)
for up to 16 LV columns
for up to 24 columns or cartridges
for 16 LV or 24 columns
50
1
1
730150
730360
730173
1
1
1
2
2
730175
730365
730176
730177
730178
730151
730174
info@mn-net.com
Pack of
REF
12
12
12
730194
730183.12
730184.12
12
12
12
2
1
730185
730152
730154
730233
730179
1
1
1
1
1
1
730187
730990
730188
730157
730366
730153
1
12
730189.1
730189.12
4
4
4
730387
730243
730386
Valves, plastic
Stainless steel needles
Polypropylene needles
PP tanks for vacuum manifold for 12 columns
Vacuum gauge, complete with accessories (3+4)
female
male
For special applications, which require maximum protection from cross contamination we supply chromeplated brass valves and stainless steel or PTFE connectors, the application of which is shown below. These
special connectors are fitted through the lid; thus the
sample only has contact with the inert connector and
can flow directly into the receptacle.
Drying attachment
12
12
730106
730564
If the eluate has to be evaporated, this can be performed with the so-called drying attachment (11, see
below). This special lid has a gas connector on one side
(12), from which the gas is fed simultaneously to the
12, 16, or 24 stations (13). Thus 12, 16, or 24 eluates
can be evaporated simultaneously by just changing the
lid and applying a stream of inert gas, e.g., nitrogen.
www.mn-net.com
51
Ordering information
Description
52
Pack of
100
50
30
20
20
20
20
20
250
250
250
250
250
250
250
250
50
30
250
250
50
50
250
250
4
10
4
10
4
10
1
REF
730159
730160
730161
730230
730380
730355
730158
730474
730164
730162
730163
730351
730034
730356
730026
730475
730171
730172
730191
730192
732500
732501
732019
732020
730104.4
730105
730100.4
730101
730350.4
730385
730566
730102
1 kit
730103
730381
1 kit
730382
730388
1 kit
730389
info@mn-net.com
High-throughput SPE
Automated and on-line SPE
Performing Solid Phase Extraction (SPE) manually can be time consuming and nerve-racking, especially when
recovery and reproducibility are lacking due to sample variability. If SPE can be reliably automated, it becomes a
much more efficient and reproducible process.
On-line SPE is a powerful method in automated sample
preparation where the SPE hardware is technically integrated into a HPLC system. Crude samples are placed
in an autosampler and processed fully automatic prior
to injection into a GC (MS) or LC (MS) system. MN offers different on-line column configurations designed
to fit your on-line SPE needs and filled with a choice of
different adsorbents, modifications and particle sizes:
EC columns
CC cartridges
Columns for Gilson ASPEC systems are readyto-use assembled with caps. In addition to the
columns and phases listed below, all 1, 3 and 6mL
CHROMABOND polypropylene columns from our
program can be supplied assembled with ASP caps.
Ordering information
Ready-to-use EC columns or ChromCart cartridges for on-line SPE (standard dimensions 20 x 2mm
or 20 x 4mm, resp.), filled with CHROMABOND
HR-Xpert phases (15m particles) or with
NUCLEODUR C18 ec, C8ec, CN (20m particles)
CHROMABOND
Columns for the Gilson ASPEC
Ordering information
Column size Weight [mg] Pack of [columns]
1mL
3mL
6mL
100
500
1000
100
100
100
www.mn-net.com
3mL
3mL
6mL
200
500
1000
50
50
30
730214MPS
730073MPS
730075MPS
1mL
3mL
3mL
100
200
500
100
50
50
730011MPS
730012MPS
730013MPS
1mL
3mL
6mL
100
200
500
30
30
30
730935MPS
730931MPS
730939MPS
CHROMABOND C18 ec
SiOH
REF
730071ASP
730073ASP
730075ASP
REF
CHROMABOND HR-X
53
High-throughput SPE
CHROMABOND MULTI 96 for robot systems
Alternatively CHROMABOND MULTI 96 plates provide a
means of high throughput sample preparation by processing 96 samples in a standard 8x12 microcolumn
plate format compatible with standard 96-well plate
liquid handling technologies and injection systems.
MULTI 96 plates are available for solid phase extraction
(SPE) and for filtration (see page 76).
CHROMABOND MULTI 96 SPE in microtiter format
96-well PP microtiter plates with PE filter elements
Cavity volume 1.5mL
Multiprobe II (Perkin-Elmer)
54
info@mn-net.com
High-throughput SPE
CHROMABOND MULTI 96 vacuum manifold
For handling of CHROMABOND MULTI 96 SPE plates for up to 96 samples
CHROMABOND MULTI 96 is designed for use in common robotic workstations or commercially available
liquid handling systems. Alternatively, use of multichannel pipetters facilitates a manual liquid transfer.
Extraction is carried our using the CHROMABOND
MULTI 96 vacuum manifold. With the help of the
control valve the vacuum of the manifold can be adjusted leading to an optimum flow rate through the
CHROMABOND MULTI 96 SPE plate.
Description
Pack of
REF
CHROMABOND MULTI 96 vacuum manifold with reservoir tank, vacuum gauge, and control valve
738630.M
10
5
5
10
30
2
738651
738650.5
738637
738652
738638
738639.M
738645
Ordering information
For CHROMAFIL MULTI 96 filter plates see page 76. The ordering information of 96-well plates packed with
individual CHROMABOND adsorbents is listed with the respective phases.
www.mn-net.com
55
Base material coarse-grained kieselguhr (also known as diatomaceous earth, hydromatrix, celite)
Large pore size, high pore volume, constantly high batch-to-batch quality
pH working range 113
Application:
liquid-liquid extraction of highly viscous aqueous solutions such as physiological fluids (blood, plasma,
and serum) in clinical chemistry, dyes in textiles, environmental and food analysis without use of a separation funnel
High water loadability without breakthrough of water during elution with organic solvents
also suited for removing small amounts of water from solvents which are not miscible with water
Liquid-liquid extraction
Advantages:
fast, reproducible and economical
simultaneous preparation of several samples
no problems with phase separation no formation of emulsions
high recovery rates
saving of time and solvents
organic solutions need not to be dried after separation
cyclohexane
toluene
56
Sample
application
Adsorption of
the sample
Sample elution
info@mn-net.com
Ordering information
Column volume
Adsorbent weight
Max. volume capacity
of aqueous solution
Pack of
1mL
250mg
3mL
500mg
6mL
1g
15mL
3g
30mL
4.5g
45mL
8.3g
70mL
14.5g
150mL
37.5g
0.25mL
0.5mL
1mL
3mL
5mL
10mL
20mL
50mL
100
50
30
30
30
30
30
10
730501
730502
730487
730489
730505
730506
730507
730509
96-well plates 96 x 150mg, packs of 1 plate, for max. 96 x 0.2mL aqueous solution
738131.150M
500g
730595.500
1kg
730595.1000
5kg
730595.5000
Liquid-liquid extraction
Sample pretreatment:
Weigh about 1g cut-up textile sample (colored textiles about
0.1g) in a 100mL threaded vial. (Degrease leather samples
before processing: cover sample with technical purity n-hexane
and put the vial in an ultrasonic bath for 20min. After decanting the n-hexane rinse with little n-hexane and dry sample by
gentle heating and blowing with air or N2.)
Add 250L internal standard (IS: 1.2mg/mL tetramethylbenzidine in methanol ethyl acetate (1:1, v/v)), 17.0mL citrate
buffer (pH 6) (25.05g citric acid and 12.64g NaOH, fill up with
deionized water to 2L) and heat 30min at 70C. Then add
3mL of a freshly prepared solution of 0.2g/mL sodium dithionite in water and heat for exactly 30min to 70C while shaking
occasionally.
Sample application:
Cool the solution immediately (put vial in water stopping of reductive cleavage). After 510min pour it onto the
CHROMABOND XTR column (squeeze textile remains).
730508
For parallel processing of up to 24 CHROMABOND XTR columns 1150mL we recommend the polypropylene rack REF 730508 consisting of:
two side walls (1), middle part including stopcocks and needles (2), bottom part (3),
top part for stabilizing 45mL and 70mL CHROMABOND XTR columns (4).
This rack can be adjusted to various heights depending on the CHROMABOND XTR
columns and the collection vials used. Each position of the middle part is equipped
with a polypropylene stopcock on the top (REF 730185) and a polypropylene needle
on the bottom (REF 730154).
For collection of the sample, vessels such as vials, test tubes, round bottom or tapered flasks, can be used. For our program of sample vials, please see the chapter
Vials and accessories from page 77.
www.mn-net.com
57
Two-phase mixtures are completely applied to the column and the phase boundary is determined without
further work. The special membrane automatically stops the flow when the lower phase has passed. The
upper phase remains in the column, thus both phases are available for further analysis.
PTS
PTL
Ordering information
Phase separation
CHROMABOND
PTS
100
730710
100
730714
100
15
100
6
30
45
70
150
CHROMABOND PTL
100
50
50
20
730720
730722
730724
730734
100
70
730718
100
15
45
730716
730730
100
30
730712
100
3
6
REF
100
50
50
730732
730736
730738
730740
730742
CHROMABOND PTL in action: organic upper phase (colorless), aqueous lower phase (red)
58
info@mn-net.com
Ordering information
Designation
Pack of
REF
1 kit
727450
1 kit
727451
complete with adapter and PTFE tap, fitted with a polyethylene net to protect against bursting
20mm
20mm
25mm
25mm
30mm
30mm
40mm
40mm
ID
ID
ID
ID
ID
ID
ID
ID
x
x
x
x
x
x
x
x
200mm
400mm
200mm
400mm
300mm
400mm
300mm
450mm
length
length
length
length
length
length
length
length
1-L eluent reservoir with adapter, covered with a protective plastic sleeve for burst protection;
this also prevents build-up of UV-induced radicals in the eluent
2-L eluent reservoir as above
Pressure gauge for controlling flow rates
PTFE tubing, 3mm OD, 2mm ID, length 1m
Sea sand, acid washed and calcined
Glass fiber wadding, silanized
www.mn-net.com
1
1
1
1
1
1
1
1
column
column
column
column
column
column
column
column
727400
727401
727402
727403
727404
727405
727406
727407
727420
1
1
1m
1000g
25g
727421
727422
727424
727423
718002
Flash chromatography
59
Increases flexibility
considerable program of different phases and modifications
Saves time and money
convenient prices, short delivery times
Flash chromatography
Technical features
CHROMABOND RS cartridges are 100% compatible with the ISCO Companion, no additional hardware is
needed for this type of purification systems.
CHROMABOND RS cartridges (except RS 800 and RS 1600 with Maxi Luers) can also be used as stand alone
system with any pump/detector/fraction collector combination using the CHROMABOND Flash Starter Kit
(REF 730798) or the CHROMABOND Flash Stand Alone Kit (REF 732903).
REF 730798
CHROMABOND
Flash Starter Kit
REF 732903
CHROMABOND
Flash Stand Alone Kit
CHROMABOND
60
Pack of
REF
1 kit
730798
info@mn-net.com
CHROMABOND
Pack of
REF
1 kit
732903
Product range designed for use in the Teledyne Isco CombiFlash systems (Companion, Rf etc.) and Flash
systems of Biotage AB (FlashMaster) without additional connectors or capillaries
On request all column types listed below can be packed with any adsorbent as described on page 8
(please note that other packings often result in differing adsorbent weights).
Designation
Column length
[cm]
ID
[mm]
Adsorbent
weight [g]
Pack of
[columns]
All CHROMABOND Flash RS cartridges can be directly used in the Teledyne Isco Companion, Rf, etc.
REF
9.8
11.6
16.5
17.1
24.0
25.5
20.0
27.0
38.5
43.0
12.4
21.2
21.2
26.4
30.8
36.0
60.0
60.0
82.0
104.0
4
15
25
40
80
120
200
330
800
1600
20
20
15
15
12
10
6
4
2
2
732800
732801
732802
732803
732804
732805
732806
732807
732808
732809
9.8
11.6
16.5
17.1
24.0
25.5
20.0
27.0
38.5
43.0
12.4
21.2
21.2
26.4
30.8
36.0
60.0
60.0
82.0
104.0
4.3
16.4
26
43
86
130
220
360
880
1760
2
1
1
1
1
1
1
1
1
1
732810
732811
732812
732813
732814
732815
732816
732817
732818
732819
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
9.8
11.6
16.5
17.1
24.0
25.5
20.0
27.0
12.4
21.2
21.2
26.4
30.8
36.0
60.0
60.0
4.3
16.4
26
43
86
130
220
360
2
1
1
1
1
1
1
1
732820
732821
732822
732823
732824
732825
732826
732827
RS
RS
RS
RS
RS
RS
RS
RS
4 NH2
15 NH2
25 NH2
40 NH2
80 NH2
120 NH2
200 NH2
330 NH2
www.mn-net.com
Flash chromatography
Ordering information
61
Flash
Flash
Flash
Flash
Flash
Flash
Flash
Flash
RS
RS
RS
RS
RS
RS
RS
RS
4 OH (Diol)
15 OH (Diol)
25 OH (Diol)
40 OH (Diol)
80 OH (Diol)
120 OH (Diol)
200 OH (Diol)
330 OH (Diol)
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
Flash
Flash
Flash
Flash
Flash
Flash
Flash
Flash
RS
RS
RS
RS
RS
RS
RS
RS
4 CN
15 CN
25 CN
40 CN
80 CN
120 CN
200 CN
330 CN
Column length
[cm]
9.8
11.6
16.5
17.1
24.0
25.5
20.0
27.0
ID
[mm]
12.4
21.2
21.2
26.4
30.8
36.0
60.0
60.0
Adsorbent
weight [g]
4.3
16.4
26
43
86
130
220
360
Pack of
[columns]
2
1
1
1
1
1
1
1
732830
732831
732832
732833
732834
732835
732836
732837
9.8
11.6
16.5
17.1
24.0
25.5
20.0
27.0
12.4
21.2
21.2
26.4
30.8
36.0
60.0
60.0
4.3
16.4
26
43
86
130
220
360
2
1
1
1
1
1
1
1
732840
732841
732842
732843
732844
732845
732846
732847
9.8
9.8
9.8
12.4
12.4
12.4
8
8
8
20
20
20
732870
732871
732872
Flash chromatography
62
REF
11.6
11.6
11.6
21.2
21.2
21.2
30
30
30
20
20
20
732874
732873
732875
17.1
17.1
17.1
26.4
26.4
26.4
80
80
80
16.5
16.5
16.5
21.2
21.2
21.2
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
CHROMABOND
Flash
Flash
Flash
Flash
Flash
Flash
Flash
Flash
Flash
Flash
Flash
Flash
Flash
Flash
Flash
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
15/2 SiOH
25/5 SiOH
25/10 SiOH
70/10 SiOH
70/20 SiOH
70/25 SiOH
150/25 SiOH
150/50 SiOH
150/70 SiOH
15/2 C18 ec
25/5 C18 ec
70/10 C18 ec
150/50 C18 ec
70/10 NH2
70/20 NH2
9.0
10.0
10.0
15.4
15.4
15.4
17.0
17.0
17.0
9.0
10.0
15.4
17.0
15.4
15.4
15.8
20.5
20.5
26.8
26.8
26.8
38.2
38.2
38.2
15.8
20.5
26.8
38.2
26.8
26.8
50
50
50
2.0
5.0
10.0
10.0
20.0
25.0
25.0
50.0
70.0
2.0
5.0
10.0
50.0
10.0
20.0
15
15
15
732876
732877
732878
15
15
15
732879
732881
732880
50
50
50
30
30
30
20
20
10
50
20
20
10
20
20
730881
730891
730666
730885
730915
730892
730667
730887
730880
730890
730884
730886
730888
730768
730767
info@mn-net.com
Due to the narrow particle size distribution, the excellent packing quality and the optimized stationary
phases (acid washed silica, reduced particulate matter)
our cartridges can realize highest loadability at best
possible separation efficiency. Additionally, the large
range of different cartridge lengths and diameters eases to find the optimum in loadability for a given sample
amount.
40g silica
gsample / gadsorbent
1%
10%
4 g: 10 mL/min
40 g: 100 mL/min
Back pressure of CHROMABOND Flash RS SiOH cartridges (eluent hexane ethyl acetate 9:1 or 8:2)
Cartridge
RS 4
RS 15
RS 25
RS 40
RS 80
RS 120
RS 200
RS 330
20mL/min
0.75bar
0.25bar
0.5bar
40mL/min
1.5bar
0.75bar
1.0bar
0.75bar
80mL/min
1.5bar
1.75bar
1.5bar
1.5bar
1.0bar
1.0bar
1.5bar
Flow rate
120mL/min 160mL/min 200mL/min 240mL/min
2.0bar
3.0bar
2.5bar
1.5bar
4.0bar
3.0bar
3.0bar
2.0bar
2.0bar
3.0bar
5.0bar
3.5bar
2.5bar
Flash chromatography
Separation
difficult
easy
3.5bar
4.0bar
3.0bar
3.0bar
4.0bar
Conditioning volumes for CHROMABOND Flash RS cartridges (normally 1.5 column volumes of the eluent)
Cartridge
RS 4
RS 15
RS 25
RS 40
RS 80
www.mn-net.com
Cartridge
RS 120
RS 200
RS 330
RS 800
RS 1600
63
Saving time and money, because expensive optimizations are not required
not OK
OK
1
front
Flash chromatography
4 5
2
start
1
not OK
4
8
50
During TLC optimization always use solvents, which are well suited for the following Flash chromatography!
MN adsorbents
As with our SPE products, all Flash columns and cartridges from MN are available with our whole range of
CHROMABOND phases (more than 40, e.g., C18, C8, OH, Alox as listed on page 8)
Additionally you can choose from our range of POLYGOPREP silica packings in particle sizes from 20 to
130m and pore sizes from 60 to 4000 (see page 203).
For high performance Flash separations you can order columns packed with spherical NUCLEODUR featuring very high separation efficiency and extremely increased column lifetime (particle size > 20m as listed
on page 198)
64
separation efficiency
price
info@mn-net.com
MN, as a manufacturer of silicas, has decades of experience in the production of first class separation phases
and columns. This leads to highest separation efficiencies of the columns, a constant back pressure (via controlled narrow particle size distribution) and good reproducibilities from cartridge to cartridge.
The separation efficiency is in the first step not influenced by the dimension or the geometry of the Flash RS
cartridges. The chromatograms below show an identical resolution and peak form for different column dimensions, when flow and sample amount is adjusted
correctly. This is advantageous for optimization and
upscaling experiments.
www.mn-net.com
15 g, 1 mL sample,
40 mL/min
25 g, 1 mL sample,
80 mL/min
40 g, 1 mL sample,
160 mL/min
Flash chromatography
4 g, 0.25 mL sample,
10 mL/min
65
Sample clarification
Syringe filters are used for filtration of suspended matter from liquid samples or gases. With CHROMAFIL,
rapid purification and removal of particles is very simple: just place the filter on the syringe, and you are
ready for filtration. Special manipulations are not required. Contamination of sensitive instrumentation by
solid impurities can be avoided, thus increasing lifetime
of chromatographic columns and equipment.
Advantages:
Polypropylene housing
Considerably better solvent stability compared to acrylate and polystyrene filters, featuring a low content of
extractable substances
Lowest content of extractable substances
The housing of every CHROMAFIL filter is ultrasonically sealed (welded), not glued, because glue may
have extractable ingredients. Welding leads to a tight
connection between both parts, thus the filter can
be used in both directions. The special thick rim of
the housing is ideal for use in laboratory robots (e.g.,
SOTAX, Benchmate).
Luer lock on the side of entry
For a safe connection on the high-pressure side every
filter provides a Luer lock on the side of entry.
Luer exit
For 25 and 3mm filters: standard Luer exit
For 15mm filters: minispike This Luer configuration
offers a low hold-up volume and easy filtration into autosampler vials and NMR tubes.
66
info@mn-net.com
Page
Polyester (PET)
Regenerated cellulose (RC)
Polytetrafluoroethylene (PTFE)
Cellulose mixed esters (MV)
Cellulose acetate (CA) sterile and non-sterile
Polyamide / Nylon (PA)
Polyethersulfone (PES)
Polyvinylidene difluoride (PVDF)
Glass fiber (GF)
69
69
70
70
71
72
71
72
73
Xtra: imprint for direct identification of the membrane type, diameter and pore size
Xtra:
Xtra:
CHROMAFIL BIG-BOX
The technology:
www.mn-net.com
Sample clarification
Polyester (GF/PET)
Regenerated cellulose (GF/RC)
Polyvinylidene difluoride (GF/PVDF)
CHROMAFIL Xtra
67
The HPLC filter with glass fiber prefilter, especially suited for mixtures of
water and organic solvents
Recommended for solutions with a high load of particulate matter or for highly
viscous solutions
Ordering information
Type
GF/PET-20/25
GF/PET-45/25
Pore size
Membrane
[m]
diameter [mm]
1.0/0.20
1.0/0.45
25
25
Color code
top
bottom
blue
orange
black
orange
Standard pack
filters/pack
REF
100
729032
100
729033
BIG-BOX
filters/pack
REF
400
729032.400
400
729033.400
Sample clarification
Ordering information
Type
GF/RC-20/25
GF/RC-45/25
Pore size
Membrane
[m]
diameter [mm]
1.0/0.20
1.0/0.45
25
25
Color code
top
bottom
blue
blue
black
blue
Standard pack
filters/pack
REF
100
729050
100
729051
BIG-BOX
filters/pack
REF
400
729050.400
400
729051.400
Recommended for filtration of biological samples with high particle loads. This
filter features a high binding capacity for proteins.
Also suited for filtration of polar and nonpolar solutions
Ordering information
Type
GF/P-45/25
68
Pore size
Membrane
[m]
diameter [mm]
1.0/0.45
25
Color code
top
bottom
black
white
Standard pack
filters/pack
REF
100
729039
BIG-BOX
filters/pack
REF
400
729039.400
info@mn-net.com
The HPLC filter, especially suited for mixtures of water and organic solvents
For TOC/DOC determination
Not cytotoxic, does not inhibit the growth of microorganisms and higher cells
Pore size
Membrane
[m]
diameter [mm]
0.20
0.45
1.2
25
25
25
labeled
labeled
labeled
pack
REF
729221
729220
729229
BIG-BOX
filters/pack
REF
400
729221.400
400
729220.400
400
729229.400
Standard
filters/pack
100
100
100
100
pack
REF
729022
729023
729021
729020
BIG-BOX
filters/pack
REF
800
729022.800
800
729023.800
400
729021.400
400
729020.400
Pore size
Membrane
[m]
diameter [mm]
PET-20/15 MS
0.20
PET-45/15 MS
0.45
PET-20/25
0.20
PET-45/25
0.45
MS = minispike on filter exit
15
15
25
25
Color code
top
bottom
yellow
orange
colorless
orange
yellow
orange
colorless
orange
Sample clarification
Standard
filters/pack
100
100
100
Pore size
Membrane
[m]
diameter [mm]
0.20
0.45
25
25
labeled
labeled
Standard pack
filters/pack
REF
100
729230
100
729231
BIG-BOX
filters/pack
REF
400
729230.400
400
729231.400
Standard
filters/pack
100
100
100
100
BIG-BOX
filters/pack
REF
800
729036.800
800
729037.800
400
729030.400
400
729031.400
Pore size
Membrane
[m]
diameter [mm]
RC-20/15 MS
0.20
RC-45/15 MS
0.45
RC-20/25
0.20
RC-45/25
0.45
MS = minispike on filter exit
www.mn-net.com
15
15
25
25
Color code
top
bottom
yellow
blue
colorless
blue
yellow
blue
colorless
blue
pack
REF
729036
729037
729030
729031
69
Very resistant towards all kinds of solvents as well as acids and bases
Pore size
Membrane
[m]
diameter [mm]
0.20
0.45
1.0
25
25
25
labeled
labeled
labeled
Sample clarification
Standard
filters/pack
100
100
100
pack
REF
729207
729205
729247
BIG-BOX
filters/pack
REF
400
729207.400
400
729205.400
400
729247.400
Standard
filters/pack
100
100
100
100
100
pack
REF
729014
729015
729008
729009
729007
BIG-BOX
filters/pack
REF
Pore size
Membrane
[m]
diameter [mm]
O-20/3
0.20
O-45/3
0.45
O-20/15 MS
0.20
O-45/15 MS
0.45
O-20/25
0.20
MS = minispike on filter exit
3
3
15
15
25
Color code
top
bottom
colorless
colorless
colorless
colorless
yellow
colorless
colorless
colorless
yellow
colorless
800
800
400
729008.800
729009.800
729007.400
Pore size
Membrane
[m]
diameter [mm]
0.20
0.45
25
25
labeled
labeled
Standard pack
filters/pack
REF
100
729206
100
729204
BIG-BOX
filters/pack
REF
400
729206.400
400
729204.400
Standard pack
filters/pack
REF
100
729006
100
729004
BIG-BOX
filters/pack
REF
400
729006.400
400
729004.400
70
Pore size
Membrane
[m]
diameter [mm]
0.20
0.45
25
25
Color code
top
bottom
yellow
yellow
colorless
yellow
info@mn-net.com
Hydrophilic membrane
for filtration of water-soluble oligomers and polymers, especially suited for
biological macromolecules
Very high shape stability in aqueous solutions
Pore size
Membrane
[m]
diameter [mm]
CA-20/25
CA-45/25
0.20
0.45
25
25
labeled
labeled
BIG-BOX
filters/pack
REF
400
729226.400
400
729227.400
Standard
filters/pack
100
100
100
100
BIG-BOX
filters/pack
REF
800
729054.800
800
729055.800
400
729026.400
400
729027.400
Sterile filters
Pore size
Membrane
[m]
diameter [mm]
0.20
0.45
0.20
0.45
CA-20/15 MS (S)
CA-45/15 MS (S)
CA-20/25 (S)
CA-45/25 (S)
MS = minispike on
0.20
0.45
0.20
0.45
filter exit
15
15
25
25
Color code
top
bottom
yellow
red
colorless
red
yellow
red
colorless
red
15
15
25
25
yellow
colorless
yellow
colorless
red
red
red
red
50
50
50
50
pack
REF
729054
729055
729026
729027
729052
729053
729024
729025
Polyethersulfone (PES)
Hydrophilic membrane
for aqueous liquids and aqueous liquids with low organic contents
Very low adsorption for pharmaceuticals and proteins
good stability against acids and bases
Sample clarification
Standard pack
filters/pack
REF
100
729226
100
729227
Pore size
Membrane
[m]
diameter [mm]
0.20
0.45
5.0
www.mn-net.com
25
25
25
labeled
labeled
labeled
Standard
filters/pack
100
100
100
pack
REF
729240
729241
729242
BIG-BOX
filters/pack
REF
400
729240.400
400
729241.400
400
729242.400
71
Pore size
Membrane
[m]
diameter [mm]
0.20
0.45
25
25
labeled
labeled
Standard pack
filters/pack
REF
100
729212
100
729213
BIG-BOX
filters/pack
REF
400
729212.400
400
729213.400
Sample clarification
Type
AO-20/3
AO-45/3
AO-20/15 MS *
AO-45/15 MS *
AO-20/25
AO-45/25
Pore size
Membrane
[m]
diameter [mm]
0.20
0.45
0.20
0.45
0.20
0.45
3
3
15
15
25
25
Color code
Standard
top
bottom
filters/pack
light beige light beige
100
light beige light beige
100
yellow
green
100
colorless
green
100
yellow
green
100
colorless
green
100
pack
REF
729010
729011
729048
729049
729012
729013
BIG-BOX
filters/pack
REF
800
800
400
400
729048.800
729049.800
729012.400
729013.400
Pore size
Membrane
[m]
diameter [mm]
0.20
0.45
25
25
labeled
labeled
Standard pack
filters/pack
REF
100
729218
100
729219
BIG-BOX
filters/pack
REF
400
729218.400
400
729219.400
Standard pack
filters/pack
REF
100
729043
100
729044
BIG-BOX
filters/pack
REF
800
729043.800
800
729044.800
Pore size
Membrane
[m]
diameter [mm]
PVDF-20/15 MS
0.20
PVDF-45/15 MS
0.45
MS = minispike on filter exit
72
15
15
Color code
top
bottom
yellow
white
colorless
white
info@mn-net.com
For solutions with high loads of particulate matter or for highly viscous solutions (e.g., soil samples, fermentation broths)
As prefilters for other CHROMAFIL filters, they prevent plugging of the membrane.
Pore size
Membrane
[m]
diameter [mm]
nom. 1.0
25
labeled
Standard pack
filters/pack
REF
100
729228
BIG-BOX
filters/pack
REF
400
729228.400
Standard pack
filters/pack
REF
100
729034
100
729028
BIG-BOX
filters/pack
REF
www.mn-net.com
15
25
Color code
top
bottom
blue
colorless
yellow
black
400
729028.400
Sample clarification
Type
73
For example, a PTFE filter with PP housing does not liberate any UV-detectable substances during filtration of
5mL THF, although PP shows only limited resistance
towards THF.
CA
RC
PA
nButanol
Cyclohexane
Dimethylformamide
Benzene
Sample clarification
Dichloromethane
Diethyl ether
1,4Dioxane
Material
PTFE PVDF
MV
PES
PET
GF
PP
2Propanol
Sodium hydroxide, 1mol/L
Tetrahydrofuran
Tetrachloromethane
Toluene
Trichloroethene
Trichloromethane (chloroform)
Urea
Water
Xylene
limited resistance
74
Ethylene glycol
Ethanol
Ethyl acetate
info@mn-net.com
Either discard the first mL or rinse the filter unit with 1mL of the solvent prior to filtration
Before filling the syringe, draw about 1mL air into the syringe in order to minimize the liquid remaining in
the filter
Start filtration with a slight pressure; this will optimize the throughput of the filter. As soon as particles accumulate on the filter, filtration will become more difficult and the pressure on the filter will increase.
Change the filter, whenever the resistance becomes too large in order to prevent rupture of the housing.
Do not apply CHROMAFIL syringe filters on humans; they are only intended for lab use!
Always use syringes 10mL; smaller syringes can easily cause pressures above the 6bar limit of the filters.
The temperature should not exceed 55C.
Do not re-use the filters.
Sample clarification
Ordering information
Description
Filtration
Filtration
Filtration
Filtration
Filtration
Filtration
Filtration
Filtration
Filtration
cartridges
cartridges
cartridges
cartridges
cartridges
cartridges
cartridges
cartridges
cartridges
PET (polyester)
PET (polyester)
RC (regenerated cellulose)
RC (regenerated cellulose)
PTFE (polytetrafluoroethylene)
PTFE (polytetrafluoroethylene)
PVDF (polyvinylidene difluoride)
PVDF (polyvinylidene difluoride)
GF (glass fiber)
www.mn-net.com
Pore size
[m]
0.20
0.45
0.20
0.45
0.20
0.45
0.20
0.45
nom. 1.0
Pack of
[cartridges]
100
100
100
100
100
100
100
100
100
Column volume
3mL
6mL
730578.320
730578.345
730068.320
730068.345
730570.320
730570.345
730579.320
730579.345
730517.3100
730578.620
730578.645
730068.620
730068.645
730570.620
730570.645
730579.620
730579.645
730517.6100
75
Sample clarification
76
Ordering information
Description
Filter plates with cellulose mixed ester filter elements (0.20m)
Filter plates with cellulose mixed ester filter elements (0.45m)
Filter plates with RC filter elements (regenerated cellulose, 0.2m)
Filter plates with RC filter elements (regenerated cellulose, 0.45m)
Filter plates with PTFE filter elements (0.2m)
Filter plates with PTFE filter elements (0.45m)
Filter plates with PTFE filter elements (1.0m)
Filter plates with PTFE filter elements (3.0m)
Filter plates with PE filter elements (20m)
Filter plates with PE filter elements (50m)
Filter plates with glass fiber filter elements (nominal 1m)
Filter plates with glass fiber filter elements (nominal 3m)
CHROMABOND MULTI 96 vacuum manifold for monoblocks, with reservoir tank, vacuum gauge,
and control valve, for filtration with 96-well filter plates
Pack of
100
100
100
Pack of
1
1
1
1
1
1
1
1
1
1
1
1
1
REF
738770.M
738771.M
738656.M
738657.M
738660.M
738661.M
738662.M
738663.M
738655.M
738659.M
738655.2M
738658.M
738630.M
REF
729100
729101
729102
info@mn-net.com
According to the high requirements of chemical analyses, especially with regard to reproducibility combined
with high detection sensitivity, the container material for the respective samples is of great importance.
In chromatography generally vials made from 1st hydrolytic class glass are being used. This type includes
borosilicate glasses like Duran, Pyrex, Fiolax, and
others. Glass of this class, often called neutral glass,
has a very good chemical resistance towards acidic and
neutral solutions. The relatively low alkali content permits good values for the resistance towards alkaline
solutions, too. Except for the snap cap vials for storage
of powdery samples the vials of our program are made
from glass of 1st hydrolytic class (manufactured in accordance with Eu.Ph., U.S.P., DAB, Ph. Jap.).
Temperature
resistance
from/to
Analytical
purity
PTFE virginal
Natural rubber/PTFE
Red Rubber/TEF (FEP)
Butyl
Butyl/PTFE
Silicone/PTFE
PTFE/Silicone/PTFE
40C/120C
40C/110C
40C/120C
40C/120C
60C/200C
60C/200C
low
medium
medium
medium
high
high
Fragmentation
due to hardness and
molecular structure
(coring)
high, big particles
medium
medium
medium
low to medium
very low
Hardness (needle
penetration)
Resealability (in
case of multiple
injections)
no resealability
high
medium
medium
medium
low to medium
very low
In HPLC the septa often need to have a (cross-) slit as a penetration aid for thick and blunt needles. Furthermore,
the slit avoids a vacuum in the vial during sample removal and thus guarantees constant volumes in case of multiple injections. The PTFE lamination protects the elastomeric carrier material of the septa from decomposition
by aggressive samples or solvents, but also in the other direction the sample from possible contaminations
through substances of the carrier material.
Compatibility charts have been compiled for the following instrument manufacturers: Agilent, CTC, Dionex,
PerkinElmer, Shimadzu, Thermo Scientific, Varian
(Agilent), VWR (Merck/Hitachi), Waters. Where applicable, each chart is divided into fields of use (GC, HPLC,
Headspace).
We generally recommend that you ask for cost-free
samples for testing purposes, as even technically comparable products may differ in their optical appearance.
www.mn-net.com
Miscellaneous
Septa guide
Except where explicitly mentioned, septa are assembled ready to use. Septa beneath or beside a cap are
shown for illustration purposes only, and they are pictured upside down.
All drawings in this chapter are scale 1:2.
77
70286
70282
31.5
31.5
5.5
70251
70212
702002
702003
40
40
40
REF
702880
30
5.5
40
40
8.2
7
Type of vial
Clear, conical
Clear, round bottom
Clear, flat bottom
Clear, conical
Amber, conical
Clear, flat bottom
Amber, flat bottom
Clear, flat bottom
Usable volume
0.2mL
0.3mL
0.8mL
0.6mL
0.6mL
0.7mL
0.7mL
1.2mL
OD
5.5
5.5
8.2
7
7
7
7
8.2
x
x
x
x
x
x
x
x
x
height
31.5mm
31.5mm
30mm
40mm
40mm
40mm
40mm
40mm
8.2
100
100
100
100
100
100
100
100
70286
70282
70251
70212
70212.1
702002
702003
702880
70252.1
Cap description
N8 aluminium crimp cap, silver, center
hole
N8 aluminium crimp cap as above
N8
N8
N8
N8
aluminium
aluminium
aluminium
aluminium
crimp
crimp
crimp
crimp
cap
cap
cap
cap
Crimping tools N8
as
as
as
as
above
above
above
above
702025
70289
702878
702800
Septum description
PTFE virginal, white
Hardness Thickness
53shoreD 0.25mm
100
70283
Natural rubber/
Butyl red-orange/TEF colorless
Red Rubber/FEP colorless
Silicone white/PTFE red
PTFE red/Silicone white/PTFE red
no liner
45shoreA
1.0mm
100
70252.1
40shoreA
40shoreA
40shoreA
1.0mm
1.0mm
1.0mm
100
100
100
100
702025
70289
702878
702800
1
1
735126
735408
78
70212.1
Pack of
info@mn-net.com
More and more replaced by screw neck vials N9, which are easier to fill due
to the wide opening compared to screw neck vials N8 with small opening
Due to the cap design not universally usable on all autosamplers in GC and
HPLC however, often used on instruments of VWR (Merck)/Hitachi, Varian,
Knauer, Gilson, Shimadzu and others
In combination with closed top screw closures also used for sample storage
(see page 92)
Description
Pack of
Screw neck vials N8, small opening (8-425 thread), and compatible inserts
70213.2
702004
702893
702968
702968.1
702974.1
702824
702005
702860
32
32
11.6
32
11.6
31
32
11.6
11.6
31
31
29
32
Type of vial
Usable volume
OD x height
Clear, flat bottom
1.5mL
11.6 x 32mm
Amber, flat bottom
1.5mL
11.6 x 32mm
Clear, flat bottom, label and scale
1.5mL
11.6 x 32mm
Amber, flat bottom, label and scale
1.5mL
11.6 x 32mm
Insert for small opening vials, clear, conical, 15mm tip
0.1mL
5 x 31mm
Insert for small opening vials, clear, conical, 9mm tip
0.15mL
5 x 31mm
Metal spring for conical inserts 5 x 31mm
100
100
100
100
100
100
100
100
100
100
the vial.
11.6
70213
70213.2
702004
702893
702968*
702968.1*
702974.1
702824
702005
702860
702068
Cap description
N8 PP screw cap, black, center hole
as above, but with closed top
N8 PP screw cap, black, center hole
as above, but with closed top
N8 PP screw cap, black, center hole
N8 PP screw cap, black, center hole
N8 PP screw cap, black, center hole
as above, but with closed top
www.mn-net.com
70245
702066
702437
Septa description
Red Rubber/FEP colorless
Red Rubber/FEP colorless
Silicone white/PTFE red
Silicone white/PTFE red
Silicone white/PTFE blue,
slit
PTFE red/Silicone white/
PTFE red
no liner
no liner
702069
70249
Hardness Thickness
70250
1.0mm
1.0mm
1.3mm
1.3mm
1.0mm
100
100
100
100
100
702067
702068
70245
702066
702437
40shoreA 1.0mm
100
702069
100
100
70249
70250
40shoreA
40shoreA
40shoreA
40shoreA
40shoreA
70213
REF
79
Illustration
Pack of
Hardness
53shoreD
40shoreA
40shoreA
40shoreA
Thickness
0.25mm
1.0mm
1.3mm
1.0mm
100
100
100
100
REF
70261
702070
70248
702481
Also available as bonded closures (advantage: thick (blunt) HPLC needles cannot push the septum into the vial)
Also available as convenient Vial Kits with 100 vials and 100 caps and as presealed vial-closure combinations
Description
Screw neck vials N9, wide opening (short thread), and compatible inserts
702282 702293
702283
702284
702813
silanized
702078
silanized
702079
silanized
702077
REF
PP
5.7
32
32
11.6
32
11.6
31
32
11.6
11.6
31
29
Type of vial
Clear, flat bottom
Amber, flat bottom
Clear, flat bottom, label and scale
as above, silanized
Amber, flat bottom, label and scale
as above, silanized
80
Pack of
31
32
Usable volume
1.5mL
1.5mL
1.5mL
1.5mL
1.5mL
1.5mL
0.2mL
0.2mL
0.25mL
0.1mL
0.3mL
1.1mL
0.2mL
0.2mL
0.15mL
1.1mL
0.3mL
32
11.6
32
32
11.6
OD
11.6
11.6
11.6
11.6
11.6
11.6
x
x
x
x
x
x
x
11.6
32
32
11.6
11.6
11.6
height
32mm
32mm
32mm
32mm
32mm
32mm
100
100
100
100
100
100
702282
702293
702283
702078
702284
702079
6 x 31mm
5.7 x 29mm
6 x 31mm
100
100
100
702716
702818
702825
6 x 31mm
6 x 31mm
11.6
11.6
11.6
11.6
x
x
x
x
32mm
32mm
32mm
32mm
11.6 x 32mm
11.6 x 32mm
100
100
702813
702077
100
100
100
100
702006
702007
702008
702135
100
100
702088
702009
info@mn-net.com
Bonded screw closures N9 (septa firmly connected with the cap, cannot be removed)
702028
REF
702027
702026
Cap description
Septa description
N9 PP bonded screw cap, blue, center hole Red Rubber/TEF colorless
N9 PP bonded screw cap, blue, center hole Silicone beige/PTFE white
N9 PP bonded screw cap, blue, center hole Silicone beige/PTFE white, slit
Pack of
Hardness Thickness
65shoreA 1.0mm
45shoreA 1.3mm
45shoreA 1.3mm
100
100
100
702028
702026
702027
100
100
100
702029
702031
702032
702031
702030
N9
N9
N9
N9
N9
N9
PP
PP
PP
PP
PP
PP
screw
screw
screw
screw
screw
screw
702732
702287
702287.1
Hardness Thickness
Septa description
PTFE virginal, white
PTFE virginal, white
PTFE virginal, white
702080
Red
Red
Red
Red
Red
Red
Rubber/FEP
Rubber/FEP
Rubber/FEP
Rubber/FEP
Rubber/FEP
Rubber/FEP
53shoreD 0.25mm
53shoreD 0.25mm
53shoreD 0.25mm
702081
colorless
colorless
colorless
colorless
colorless
colorless
702036
40shoreA
40shoreA
40shoreA
40shoreA
40shoreA
40shoreA
702037
702033
702082
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
100
100
100
100
100
100
702030
702732
702080
702081
702082
702033
702034
702038
702107
N9
N9
N9
N9
N9
N9
N9
PP
PP
PP
PP
PP
PP
PP
screw
screw
screw
screw
screw
screw
screw
702288
N9
N9
N9
N9
N9
N9
PP
PP
PP
PP
PP
PP
screw
screw
screw
screw
screw
screw
cap,
cap,
cap,
cap,
cap,
cap,
702286
702288.1
www.mn-net.com
Silicone
Silicone
Silicone
Silicone
Silicone
Silicone
Silicone
white/PTFE
white/PTFE
white/PTFE
white/PTFE
white/PTFE
white/PTFE
white/PTFE
702039
Silicone
Silicone
Silicone
Silicone
Silicone
Silicone
white/PTFE
white/PTFE
white/PTFE
white/PTFE
white/PTFE
white/PTFE
702084
red
red
red
red
red
red
red
40shoreA
40shoreA
40shoreA
40shoreA
40shoreA
40shoreA
40shoreA
702040
blue,
blue,
blue,
blue,
blue,
blue,
slit
slit
slit
slit
slit
slit
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
100
100
100
100
100
100
100
702083
702287
702287.1
702036
702037
702038
702107
702034
702109
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
100
100
100
100
100
100
702288
702288.1
702039
702040
702083
702109
40shoreA 1.0mm
100
702286
40shoreA 1.0mm
40shoreA 1.0mm
40shoreA 1.0mm
100
100
100
702035
702084
702085
40shoreA
40shoreA
40shoreA
40shoreA
40shoreA
40shoreA
Cap description
N9 PP screw cap, transparent, center hole
N9 PP screw cap, blue, center hole
N9 PP screw cap blue, closed top
702032
702085
81
Illustration
Hardness
53shoreD
40shoreA
40shoreA
Pack of
Thickness
0.25mm
1.0mm
1.0mm
100
100
100
REF
702043
702041
702042
Vial
702282:
1.5mL, clear, flat bottom
702283:
1.5mL, clear, flat bottom,
label and scale
702284:
1.5mL, amber, flat bottom,
label and scale
702009:
0.3mL, PP, transparent, with
inner cone
702287.1
702288.1
702732
702201
702204
702207
702202
702205
702203
702206
702026
702027
702208
702211
702213
702209
702212
702214
702226
32mm,
32mm,
32mm,
32mm,
32mm,
Closure description
pre-screwed with 702732:
N 9 PP screw cap, blue, center hole,
Red Rubber/FEP colorless, 40 shore A, 1.0mm
pre-screwed with 702732:
N 9 PP screw cap, blue, center hole,
Red Rubber/FEP colorless, 40 shore A, 1.0mm
pre-screwed with 702287.1:
N 9 PP screw cap, blue, center hole,
Silicone white/PTFE red, 40 shore A, 1.0mm
pre-screwed with 702288.1:
N 9 PP screw cap, blue, center hole,
Silicone white/PTFE blue, slit, 40 shore A,
1.0mm
pre-screwed with 702288.1:
N 9 PP screw cap, blue, center hole,
Silicone white/PTFE blue, slit, 40 shore A,
1.0mm
Pack of
100
REF
702857
100
702858
100
702874
100
702863
100
702873
82
info@mn-net.com
Due to the cap height not universally suitable for all instruments
Description
Screw neck vials N10, wide opening (10-425 thread), and compatible inserts
702012
702013
702813
702716
702818
silanized
702077
32
32
11.6
11.6
11.6
31
Type of vial
Clear, flat bottom
Clear, flat bottom, label and scale
Amber, flat bottom, label and scale
Insert for wide opening vials, clear, conical, 15mm tip
as above, silanized
Insert for wide opening vials, clear, conical, 12mm tip
Insert for wide opening vials, clear, with plastic spring
Insert for wide opening vials, clear, flat bottom
702825
5.7
31
32
REF
31
29
Usable volume
1.5mL
1.5mL
1.5mL
0.2mL
0.2mL
0.25mL
0.1mL
0.3mL
OD
11.6
11.6
11.6
6
6
6
5.7
6
x
x
x
x
x
x
x
x
x
height
32mm
32mm
32mm
31mm
31mm
31mm
29mm
31mm
100
100
100
100
100
100
100
100
702011
702012
702013
702813
702077
702716
702818
702825
702044
702045
702046
702047
Cap description
Septa description
N10 PP bonded screw cap*, black, center
Red Rubber/TEF colorless
hole
N10 PP bonded screw cap* as above
Silicone white/PTFE beige
N10 PP bonded screw cap* as above
Silicone white/PTFE red
N10 PP bonded screw cap* as above
Silicone white/PTFE blue, slit
* Septum firmly connected with the cap, cannot be removed
N10 PP screw cap, black, center hole
PTFE red/Silicone white/PTFE
red
N10 PP screw cap, black, center hole
no liner
www.mn-net.com
702048
702049
Hardness Thickness
65shoreA 1.0mm
100
702044
45shoreA 1.5mm
45shoreA 1.5mm
45shoreA 1.5mm
100
100
100
702045
702046
702047
45shoreA 1.0mm
100
702048
100
702049
702011
Pack of
83
Pack of
32
31
32
11.6
70201CG
Type of vial
Clear, flat bottom, small opening
Amber, flat bottom, small opening
Insert for small opening vials, clear,
Insert for small opening vials, clear,
Insert for small opening vials, clear,
Insert for small opening vials, clear,
702968
31
29
702968.1
Usable volume
1.5mL
1.5mL
0.1mL
0.15mL
0.1mL
0.25mL
OD
11.6
11.6
5
5
5
5
x
x
x
x
x
x
x
702824
702005
height
32mm
32mm
31mm
31mm
29mm
31mm
100
100
100
100
100
100
70201CG
70214CG
702968*
702968.1*
702824
702005
* Optionally you may use metal springs 702974.1 in combination with these products to push them up in the vial.
32
32
11.6
70201HP
31
32
11.6
702885
702892
702813
silanized 702075
silanized 702076
silanized 702077
29
31
11.6
Type of vial
Clear, flat bottom, wide opening
Clear, flat bottom, wide opening, label and scale
as above, silanized
Amber, flat bottom, wide opening, label and scale
as above, silanized
Insert for wide opening vials, clear, conical, 15mm tip
as above, silanized
Insert for wide opening vials, clear, conical, 12mm tip
84
31
11.6
70214CG
REF
Usable volume
1.5mL
1.5mL
1.5mL
1.5mL
1.5mL
0.2mL
0.2mL
0.25mL
0.1mL
0.3mL
31
702716
702818
OD
11.6
11.6
11.6
11.6
11.6
6
6
x
x
x
x
x
x
x
x
height
32mm
32mm
32mm
32mm
32mm
31mm
31mm
6 x 31mm
5.7 x 29mm
6 x 31mm
702825
100
100
100
100
100
100
100
100
100
100
70201HP
702885
702075
702892
702076
702813
702077
702716
702818
702825
info@mn-net.com
32
11.6
702888
32
11.6
702891
Pack of
32
11.6
702014
32
11.6
702134 PP/glass
11.6
702015
32
11.6
11.6
702016
702141
Usable volume
1.1mL
OD x height
11.6 x 32mm
100
702888
0.2mL
0.2mL
0.15mL
11.6 x 32mm
11.6 x 32mm
11.6 x 32mm
100
100
100
702891
702014
702134
1.1mL
1.1mL
1.1mL
11.6 x 32mm
11.6 x 32mm
11.6 x 32mm
100
100
100
702015
702016
702141
70231
702001 702730
Cap description
N11 aluminium crimp cap, silver,
center hole
N11 crimp cap as above, silver
N11 crimp cap as above, silver
N11 crimp cap as above, silver
N11 crimp cap as above, silver
N11 crimp cap as above, green
N11 crimp cap as above, red
N11 crimp cap as above, blue
N11 crimp cap as above, gold
N11 crimp cap as above, silver
N11 crimp cap as above, silver
N11 crimp cap as above, silver
N11 magnetic crimp cap, gold,
center hole
N11 aluminium crimp cap, silver,
center hole
Septa description
PTFE virginal, white
Hardness Thickness
53shoreD 0.25mm
100
70284
45shoreA 1.3mm
100
70231
60shoreA 1.0mm
100
702001
40shoreA 1.0mm
45shoreA 1.0mm
100
100
702730
70256
45shoreA
45shoreA
45shoreA
45shoreA
40shoreA
40shoreA
40shoreA
55shoreA
1.0mm
1.0mm
1.0mm
1.0mm
1.3mm
1.5mm
1.0mm
1.0mm
100
100
100
100
100
100
100
100
70231.1
70231.2
70231.3
70231.4
70288
702823
702995
702879
100
702801
100
702401
100
100
100
100
70262
702065
70263
70264
no liner
Type of vial
Micro-vial, clear, flat bottom
15L funnel in solid glass bottom
Micro-vial, clear, with integrated 0.2mL insert
Micro-vial, amber, with integrated 0.2mL insert
Micro-vial, polypropylene, transparent, with integrated
0.15mL glass insert, conical
Micro-vial, clear, conical with round pedestal glass plate
Micro-vial, amber, conical with round pedestal glass plate
Micro-vial, clear, conical
32
REF
www.mn-net.com
Illustration
Hardness
53shoreD
40shoreA
40shoreA
40shoreA
Thickness
0.25mm
1.0mm
1.3mm
1.0mm
85
Vial
70201HP:
1.5mL, clear, flat bottom
702885:
1.5mL, clear, flat bottom,
label and scale
702892:
1.5mL, amber, flat bottom,
label and scale
70288
702995
70256
702215
702218
702222
702216
702219
702223
702217
702221
702224
Closure description
crimped with 70256:
N 11 aluminium crimp cap, silver, center hole,
Natural rubber/Butyl red-orange/TEF colorless,
45 shore A, 1.0mm
crimped with 70256:
N 11 aluminium crimp cap, silver, center hole,
Natural rubber/Butyl red-orange/TEF colorless,
45 shore A, 1.0mm
crimped with 70256:
N 11 aluminium crimp cap, silver, center hole,
Natural rubber/Butyl red-orange/TEF colorless,
45 shore A, 1.0mm
REF
702881
100
702101HP
100
702859
Pack of
1
1
1
1
REF
735211
735311
735111
Pneumatic crimping tool for 11mm aluminium crimp caps (complete with hand switch)
Pneumatic crimping tool for 11mm aluminium crimp caps (complete with foot switch)
Crimping head N 11 (without pneumatic basic tool)
Decapping head N 11 (without pneumatic basic tool)
Pneumatic basic tool with hand switch
Pneumatic basic tool with foot switch
For more crimping tools N11 see pages 100 and 101
86
Pack of
100
1
1
1
1
1
1
735911
735114
735117
735121
735434.1
735124
735125
info@mn-net.com
0.3mL PP snap ring vial for special applications, e.g., in ion chromatography
Pack of
702713
702712
702813
702716
702818
silanized
702077
32
32
11.6
31
32
11.6
11.6
29
Type of vial
Clear, flat bottom
Clear, flat bottom, label and scale
Amber, flat bottom, label and scale
Insert for wide opening
as above, silanized
Insert for wide opening
Insert for wide opening
Insert for wide opening
www.mn-net.com
702709
702134
PP/glass
702809
PP
5.7
31
702825
31
11.6
Usable volume
1.5mL
1.5mL
1.5mL
32
32
11.6
100
100
100
702714
702713
702712
0.2mL
0.15mL
11.6 x 32mm
11.6 x 32mm
0.3mL
11.6 x 32mm
6
6
6
5.7
6
x
x
x
x
11.6
height
32mm
32mm
32mm
0.2mL
0.2mL
0.25mL
0.1mL
0.3mL
OD
11.6
11.6
11.6
32
x
x
x
x
x
31mm
31mm
31mm
29mm
31mm
100
100
100
100
100
702813
702077
702716
702818
702825
100
100
702709
702134
100
702809
702714
REF
87
702063
702710
702710.1
702064
702717.2
Cap description
Septa description
N11 PE snap ring cap, blue, center hole Red Rubber/TEF colorless
as above, transparent cap
Red Rubber/TEF colorless
N11 PE snap ring cap, blue, center hole Silicone white/PTFE red
as above, transparent cap
Silicone white/PTFE red
N11 PE snap ring cap, blue, center hole Silicone white/PTFE blue, cross-slit
as above, transparent cap
Silicone white/PTFE blue, cross-slit
N11 PE snap ring cap, blue, center hole PTFE red/Silicone white/PTFE red
as above, transparent cap
PTFE red/Silicone white/PTFE red
N11 PE cap, transparent, closed top, with thin piercing area
Pack of
702718
Hardness
65shoreA
65shoreA
55shoreA
55shoreA
55shoreA
55shoreA
45shoreA
45shoreA
702718.1
Thickness
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
1.0mm
100
100
100
100
100
100
100
100
100
REF
702401
702063
702731
702710.1
702710
702717.2
702064
702718.1
702718
702401
702710
702064
702731
702718
702225
702228
702232
702235
702719
702229
702233
702236
702227
702231
702234
702237
Containers for screw neck vials N8/N9/N10 as well as crimp neck and snap
ring vials N11
Description
81 position container blue for vials 11.6 x 32mm,
outer length 130mm, outer width 130mm, outer height 45mm,
coded, with transparent lid (suitable for freezers)
88
Pack of
1
REF
702514
info@mn-net.com
40
Pack of
43
35
13.75
11
70255
11
70203
70258
Usable volume
1mL
2mL
2mL
OD
11
13.75
11
Ready assembled crimp closures N13 and plain crimp caps N13
70257
70232
702802
x
x
x
x
height
40mm
35mm
43mm
100
100
100
702803
70255
70203
70258
702820
Cap description
N13 aluminium crimp cap, silver, center
hole
Septa description
Butyl dark gray/PTFE gray
Hardness Thickness
50shoreA
2mm
100
70257
50shoreA
2mm
100
70232
no liner
100
702802
no liner
100
702803
45shoreA
100
702820
1
1
1
735113
735133
735913
Stoppers N13
Type of vial
Clear, conical
Clear, flat bottom
Clear, flat bottom
REF
Description
49 position container blue for crimp and screw neck vials N13,
outer length 130mm, outer width 130mm, outer height 50mm,
with transparent lid (suitable for freezers)
www.mn-net.com
Pack of
1
REF
702515
89
In combination with closed top screw closures suitable for sample storage
(see page 92)
Compatible insert requires metal spring for centrical alignment
Range of ready assembled closures and plain caps with center hole or with
closed top as well as separate septa (PTFE virginal, Red Rubber/FEP and Silicone/PTFE) are available.
Description
Pack of
45
45
14.75
702962
40
45
14.75
14.75
702973
702089
Type of vial
Clear, flat bottom
Amber, flat bottom
Amber, flat bottom, label and scale
Insert, clear, conical, metal spring required
Metal spring for 702972
REF
702972
Usable volume
4mL
4mL
4mL
0.3mL
OD
14.75
14.75
14.75
6
x
x
x
x
x
height
45mm
45mm
45mm
40mm
702974
100
100
100
100
100
702962
702973
702089
702972
702974
702103
702050
702051
702926
Cap description
Septa description
N13 screw cap (13-425), green, closed top F217 white/PTFE beige
(firmly fixed)
N13 PP screw cap, black, center hole
Red Rubber/FEP colorless
as above, but closed top
Red Rubber/FEP colorless
N13 PP screw cap, black, center hole
Silicone white/PTFE red
as above, but closed top
Silicone white/PTFE red
N13 PP screw cap, black, center hole
no liner
as above, but closed top
no liner
702052
702963
Hardness Thickness
1.5mm
40shoreA
40shoreA
40shoreA
40shoreA
702966
100
702103
1.5mm
1.5mm
1.3mm
1.3mm
100
100
100
100
100
100
702050
702051
702926
702052
702963
702966
Thickness
0.25mm
1.5mm
100
100
70260
702053
90
Illustration
Hardness
53shoreD
40shoreA
40shoreA
1.3mm
100
702292
info@mn-net.com
Pack of
REF
N20
60
46
46
33
14
20
20
14
702220
702230
702240
702926
702926
702280
702280
Usable volume
0.25mL
OD x height
14 x 33mm
702210
0.75mL
14 x 46mm
702220
3mL
20 x 46mm
702230
4.5mL
20 x 60mm
702240
Replacement screw caps with septa for micro reaction vials N13 and N20
Cap description
Septa description
Hardness
Thickness
1.3mm
100
702926
55shoreA
1.4mm
48
702280
100
48
702292
702300
40shoreA
702210
Replacement septa N12 and N18 for screw caps N13 and N20, respectively
Material
Silicone white/PTFE red
Butyl red/PTFE gray
www.mn-net.com
Illustration
Hardness
40shoreA
55shoreA
Thickness
1.3mm
1.4mm
91
Available neck sizes N8, N9, N13, N15, N18 and N20
70213
N9
70213.2
702004
can be used with
702068
702893
702066
702282
702032
702293
702283
can be used with
702033
Description
Type of vial
Clear, flat bottom
Amber, flat bottom
Clear, flat bottom, label and scale
Amber, flat bottom, label and scale
Cap description
N8 PP screw cap, black, closed top
N8 PP screw cap, black, closed top
For drawings of vials see page 79
Description
Type of vial
Clear, flat bottom
Amber, flat bottom
Clear, flat bottom, label and scale
as above, silanized
Amber, flat bottom, label and scale
as above, silanized
Cap description
N9 PP screw cap blue, closed top
N9 PP screw cap blue, closed top
N9 PP screw cap blue, closed top
For drawings of vials see page 80
702284
702962
702034
702973
702089
can be used with
702103
Dimensions
Usable volume
1.5mL
1.5mL
1.5mL
1.5mL
Septa description
Red Rubber/FEP colorless
Silicone white/PTFE red
92
N13
OD
11.6
11.6
11.6
11.6
Septa description
PTFE virginal, white
Red Rubber/FEP colorless
Silicone white/PTFE red
702052
Pack of
x
x
x
x
x
height
32mm
32mm
32mm
32mm
Hardness Thickness
40shoreA 1.0mm
40shoreA 1.3mm
Dimensions
Usable volume
1.5mL
1.5mL
1.5mL
1.5mL
1.5mL
1.5mL
702051
REF
100
100
100
100
70213
70213.2
702004
702893
100
100
702068
702066
Pack of
OD
11.6
11.6
11.6
11.6
11.6
11.6
x
x
x
x
x
x
x
height
32mm
32mm
32mm
32mm
32mm
32mm
Hardness
53shoreD
40shoreA
40shoreA
Thickness
0.25mm
1.0mm
1.0mm
REF
100
100
100
100
100
100
702282
702293
702283
702078
702284
702079
100
100
100
702032
702033
702034
info@mn-net.com
Dimensions
Type of vial
Clear, flat bottom
Amber, flat bottom
Amber, flat bottom, label and scale
Pack of
Usable volume
4mL
4mL
4mL
Cap description
Septa description
N13 screw cap (13-425), green, closed top F217 white/PTFE beige
(firmly fixed)
N13 PP screw cap, black, closed top
Red Rubber/FEP colorless
N13 PP screw cap, black, closed top
Silicone white/PTFE red
For drawings of vials see page 90
x
x
x
x
height
45mm
45mm
45mm
Hardness Thickness
1.5mm
40shoreA 1.5mm
40shoreA 1.3mm
N18
16.6
18.5
100
702103
100
100
702051
702052
86
22.7
20.6
702096/702311
70285/702097
702098
702099
702104
702104
702105
702106
Type of vial
Screw neck vial
Screw neck vial
Screw neck vial
Screw neck vial
Screw neck vial
Screw neck vial
N15
N15
N15
N15
N18
N20
(15-425
(15-425
(15-425
(15-425
(18-400
(20-400
thread),
thread),
thread),
thread),
thread),
thread),
REF
N20
71
66
61
702962
702973
702089
Pack of
Screw neck vials N15, N18, and N20 for storage of liquid samples
N15
100
100
100
Usable volume
8mL
8mL
12mL
12mL
16mL
24mL
OD x height
16.6 x 61mm
16.6 x 61mm
18.5 x 66mm
18.5 x 66mm
20.6 x 71mm
22.7 x 86mm
Septa description
N15 screw cap (15-425), green, closed top F217 white/PTFE beige
(firmly fixed)
N18 screw cap (18-400), green, closed top F217 white/PTFE beige
(firmly fixed)
N20 screw cap (20-400), green, closed top F217 white/PTFE beige
(firmly fixed)
100
100
100
100
100
100
702096
702311
70285
702097
702098
702099
1.5mm
100
702104
1.5mm
100
702105
1.5mm
100
702106
Thickness
Description
OD
14.75
14.75
14.75
REF
For screw neck vials with even larger volumes please see page 102.
www.mn-net.com
93
Pack of
Snap cap vials N18 and N22 for storage of powdery samples
REF
65
40
for 70271
and 70272:
50
22
20
70271
for 702019
and 70273:
48
26
26
70272
70274
702019
70273
70275
flat
flat
flat
flat
Usable volume
5mL
10mL
15mL
25mL
bottom
bottom
bottom
bottom
PE snap caps
OD
20
22
26
26
x
x
x
x
x
height
40mm
50mm
48mm
65mm
100
100
100
100
70271
70272
702019
70273
100
100
70274
70275
40
for 70202.1
and 702017:
40
8.2
REF
for 702018:
31.5
11.6
8.2
70202.1
Pack of
702017
702807
702018
702054
Shell vials
Type of vial
N8, clear, flat bottom
N8, amber, flat bottom
N12, clear, flat bottom
PE plugs
94
Usable volume
1mL
1mL
2mL
OD
8.2
8.2
11.6
x
x
x
x
height
40mm
40mm
31.5mm
100
100
100
70202.1
702017
702018
100
100
702807
702054
info@mn-net.com
High tightness and better reproducibility of the sealing process (as compared
to crimping)
REF
75.5
46
22.5
22.5
702866
702826
Type of vial
Clear, rounded bottom
Clear, rounded bottom
702827
Usable volume
10mL
20mL
702055
702072
OD x height
22.5 x 46mm
22.5 x 75.5mm
100
100
702866
702826
Hardness Thickness
45shoreA 1.5mm
100
702827
55shoreA
65shoreA
100
100
702055
702072
100
702981
Septa description
1.5mm
1.5mm
Illustration
Hardness Thickness
45shoreA 1.5mm
55shoreA
1.5mm
Pack of
702110
100
Containers for screw neck vials N18 and crimp neck vials N20
Description
25 position container blue, with removable divider for headspace
screw neck vials N 18 and crimp neck vials N 20; outer length
130mm, outer width 130mm, outer height 80mm, with transparent lid (suitable for freezers)
www.mn-net.com
Pack of
1
REF
702516
95
Please consider our various crimping tools on page 100 and 101.
Description
70204.36
38
70215.36
38.25
38
20.5
702917
20.5
702020
54.5
38.25
22
70205.36
702918
54.5
20.5
21.7
Type of vial
Clear, flat bottom, flat DIN crimp neck (Varian)
Amber, flat bottom, flat DIN crimp neck (Varian)
Clear, rounded bottom, beveled HS crimp neck (PerkinElmer)
Clear, flat bottom, beveled HS crimp neck (Metrohm, Karl-Fischer
titration)
Clear, flat bottom, flat DIN crimp neck (Varian)
Amber, flat bottom, flat DIN crimp neck (Varian)
Clear, flat bottom, flat DIN crimp neck (Dani, Agilent)
Clear, rounded bottom, beveled HS crimp neck (CTC)
70216.36
Pack of
46
702924
46
22.5
20.5
REF
22.5
Usable volume
5mL
5mL
6mL
5mL
OD
20.5
20.5
22.0
21.7
x
x
x
x
x
height
38mm
38mm
38.25mm
38.25mm
100
100
100
100
70204.36
70215.36
702917
702020
10mL
10mL
10mL
10mL
20.5
20.5
22.5
22.5
x
x
x
x
54.5mm
54.5mm
46mm
46mm
100
100
100
100
70205.36
70216.36
702918
702924
1
1
1
1
1
735220
735320
735120
735132
735920
For electronic crimping tools N20 see page 101; pneumatic crimping tools are available on request.
96
info@mn-net.com
70206.36
75.5
23.25
70254
75.5
75,5
702541
75.5
75,5
23.25
23
702540
702261
75.5
75.5
75,5
23
23
Type of vial
Clear, flat bottom, flat DIN crimp neck
Amber, flat bottom, flat DIN crimp neck
Clear, rounded bottom, beveled HS crimp neck (PerkinElmer)
Amber, rounded bottom, beveled HS crimp neck (PerkinElmer)
Clear, rounded bottom, beveled HS crimp neck, label (PerkinElmer)
Clear, flat bottom, flat DIN crimp neck (Dani, Agilent)
Clear, rounded bottom, flat DIN crimp neck (CTC)
Clear, rounded bottom, beveled HS crimp neck, graduation at
15mL
702263
75.5
22.5
Usable volume
20mL
20mL
20mL
20mL
20mL
20mL
20mL
20mL
702086
75
22.5
OD
23.25
23.25
23
23
23
22.5
22.5
22
x
x
x
x
x
x
x
x
x
REF
height
75.5mm
75.5mm
75.5mm
75.5mm
75.5mm
75.5mm
75.5mm
75mm
22
100
100
100
100
100
100
100
100
70206.36
70217.36
70254
702541
702540
702261
702263
702086
100
100
100
88
70210.36
70208.36
702100
70209.1
101
101
75.5
70217.36
Pack of
94.5
65
30
31
70210.36
Type of vial
Clear, flat bottom, flat DIN crimp neck
Clear, flat bottom, flat DIN crimp neck
Amber, flat bottom, flat DIN crimp neck
Clear, flat bottom, flat DIN crimp neck (3rd hydrolytic class)
www.mn-net.com
51.6
31
70208.36
702100
Usable volume
25mL
50mL
50mL
100mL
70209.1
OD
30
31
31
51.6
x
x
x
x
x
height
65mm
101mm
101mm
94.5mm
97
Septa description
702773
702775
70234.9
70234
702056
as above
as above
N20 aluminium crimp cap, gold,
center hole
702093
702094
no liner
702804
50shoreA
3mm
100
702773
50shoreA
50shoreA
50shoreA
50shoreA
3mm
3mm
3mm
3mm
100
100
100
100
702775
70234.9
70234
702056
37shoreA
70237
40shoreA
3mm
100
each
100
40shoreA
3mm
100
100
100
702094
702804
702112
(separate parts)
as above
70237
REF
702093
702836
702829
70234.8
702071
702927
no liner
702835
702799
50shoreA
3mm
100
702836
50shoreA
50shoreA
50shoreA
40shoreA
3mm
3mm
3mm
3mm
100
100
100
100
702829
70234.8
702071
702927
40shoreA
3mm
100
100
702835
702799
70236
(separate parts)
no liner
no liner
70236.1
50shoreA
37shoreA
3mm
100
100
each
100
70233
70236
70236.1
70238
N20 aluminium complete tear off cap, silver Butyl dark gray/PTFE gray*
N20 aluminium complete tear off cap, silver Butyl stopper gray, unassembled
(separate parts)
98
no liner
702805
50shoreA
37shoreA
3mm
100
100
each
100
70235
70238
702805
info@mn-net.com
Septa description
702838
702834
REF
no liner
702837
702833
50shoreA
3mm
100
702838
40shoreA
40shoreA
3mm
3mm
100
100
100
702834
702837
702833
702774
702928.9
no liner
702929
702808
50shoreA
3mm
100
702774
50shoreA
50shoreA
40shoreA
3mm
3mm
3mm
100
100
100
100
702928
702928.9
702929
702808
Hardness
50shoreA
Thickness
3mm
100
70277
50shoreA
3mm
100
702101
100
702780
Illustration
50shoreA
50shoreA
40shoreA
40shoreA
50shoreA
Stopper N20
3mm
3mm
3mm
3mm
3mm
100
702057
100
702D20TB
100
70278
100
70279
Butyl gray
37shoreA
100
702931
Bromobutyl red
45shoreA
100
702931.1
702928
PE caps N20
Description
Illustration
PE caps N20
height 8.4mm
70266
702128
Hardness
height 9.1mm
Thickness Pack of
70267
N20 PE cap, transparent, for beveled HS crimp neck N20, 4.3mm center hole (no liner)
as above, but with assembled septum natural rubber red-orange/TEF colorless, 45 shore A, 1.3mm
N20 PE cap, transparent, for flat DIN crimp neck N20, 4.3mm center hole (no liner)
as above, but with assembled septum natural rubber red-orange/TEF colorless, 45 shore A, 1.3mm
www.mn-net.com
55shoreA
45shoreA
1.3mm
1.3mm
REF
702129
100
100
100
100
70266
702128
70267
702129
100
70269
100
702904
99
Crimping tools
Manual crimping tools
Advanced ergonomic version
Description
Pack of
REF
1
1
735211
735220
1
1
735311
735320
1
1
1
1
1
1
735126
735111
735113
735133
735120
735132
Manual
Manual
Manual
Manual
decapper
decapper
decapper
decapper
1
1
1
1
735408
735911
735913
735920
100
Standard version
for
for
for
for
info@mn-net.com
Crimping tools
Electronic crimping tools
Battery-powered electronic crimping tools
Long lasting lithium ion cell batteries (full battery charge for several hundred vials, life time
of battery > 1500 charges)
CE certificate of conformity along with one
year warranty
Description
Pack of
REF
1
1
735511
735520
1
1
735611
735620
735500
735700
Crimping head for 11 mm crimp caps (for electronic high power crimping tool 735700)
Crimping head for 20 mm crimp caps (aluminium, magnetic, bi-metal)
(for electronic high power crimping tool 735700)
Decapping head for 11 mm crimp caps (for electronic high power crimping tool 735700)
Decapping head for all 20 mm crimp caps (for electronic high power crimping tool 735700)
Stand for electronic crimping tools
1
1
735711
735720
1
1
1
735811
735820
735501
www.mn-net.com
101
Often called EPA vials, since they are defined in the regulations of the US
Environmental Protection Agency
Due to their size mainly used as bonded closure for a firm fit of the septum
Recommended for environmental analysis: screw closure with center hole and
Silicone/PTFE septum
Description
REF
140
95
72.5
57
27.5
702021 / 702022
27.5
702132 / 702133
27.5
Usable volume
20mL
20mL
40mL
40mL
27.5
702023 / 702024
Type of vial
Clear, flat bottom
Amber, flat bottom
102
Pack of
30mL
30mL
60mL
60mL
702074 / 702131
OD x height
27.5 x 57mm
27.5 x 57mm
Pack of
100
100
27.5 x 95mm
27.5 x 95mm
100
100
27.5 x 72.5mm
27.5 x 72.5mm
27.5 x 140mm
27.5 x 140mm
100
100
100
100
info@mn-net.com
REF
702021
702022
702132
702133
702023
702024
702074
702131
Pack of
Screw closures N24, plain screw caps N24 and single septa N22
702058
702059
702073
702102
702060
Hardness Thickness
45shoreA 3.2mm
45shoreA 3.2mm
65shoreA 2.5mm
702061
100
100
100
702058
702059
702073
702130
702102
702060
702061
702062
702791
50shoreA
50shoreA
2.4mm
2.4mm
45shoreA
3.2mm
100
100
100
100
100
50shoreA
2.4mm
100
Autosampler compatibility
Agilent
Application/Type of vial
GC:
N8 crimp (microsampling)
Page
Inserts:
Closures:
702813, 702818,
702825, 702077
702732, 702082,
702081, 702080,
702287.1, 702038,
702037, 702035,
702085, 702084,
702028, 702026
80
702813, 702818,
702825, 702077
70256, 70231.3,
70231.1, 70231.2,
702001, 702730,
70288, 702995,
702879 (for GC PAL)
84
As indicated under GC, but additionally the following closures with slit
septum: 702288.1, 702083, 702040, 702027
80
70282, 70286
702282,
702283,
702006,
702079,
702008,
702293,
702284,
702078,
702007,
702088
70201HP, 702885,
702892, 702888,
702075, 702076,
702891, 702014,
702015, 702016
70289
78
Cap description
Septum description
N24 PP bonded* screw cap, white, center hole Silicone white/PTFE beige
as above, but closed top
Silicone white/PTFE beige
N24 PP bonded* screw cap, white, center hole Red Rubber/TEF colorless
* Septum firmly connected with the cap, cannot be removed
N24 PP screw cap, white, center hole
Butyl red/PTFE gray
as above, but closed top
Butyl red/PTFE gray
N24 PP screw cap, white, center hole
no liner
as above, but closed top
no liner
N22 septum, Silicone natural/PTFE beige
702130
REF
HPLC:
N9 screw (standard samples)
N11 crimp (standard samples)
N11 snap ring (standard samples)
Headspace:
N18 screw (Combi PAL + G 1888A)
N20 crimp
www.mn-net.com
As indicated under GC, but additionally the following closure with slit
septum: 702823
702714, 702713,
702712, 702709
702813, 702818,
702825, 702077
702063, 702731,
702710.1, 702064,
702718, 702401
Vials:
Closures:
702918, 702261,
702263
70234, 702094,
702093, 702071,
702835, 702927
702866, 702826
702055
84
87
95
96
103
Autosampler compatibility
CTC
Application/Type of vial
GC:
Vials:
Inserts:
Closures:
70289, 702878
78
702282, 702293,
702283, 702284,
702006, 702078,
702079
702813, 702818,
702825, 702077
702287.1, 702035,
702026, 702027
80
70201HP, 702885,
702892, 702888,
702075, 702076
702813, 702818,
702825, 702077
84
As indicated under GC, but additionally the following closures with slit
septum: 702288.1, 702027
80
N8 crimp (microsampling)
HPLC:
70282, 70286,
70212, 70212.1,
702002, 702003
Page
70201HP, 702885,
702892, 702888,
702075, 702076
702813, 702818,
702825, 702077
70288, 702995,
702823
84
702714, 702713,
702712
702813, 702818,
702825, 702077
702710.1, 702717.2,
702718.1
87
702866, 702826
702055, 702827
702924, 702263
702929, 702834
95
Headspace:
N18 screw (Combi PAL)
N20 crimp
96
Dionex
Application/Type of vial
HPLC:
N8 screw (microsampling)
702880, 70286,
70282
Vials:
Inserts:
70213, 70213.2,
702004, 702893,
702860
702282,
702283,
702006,
702079,
702008
Closures:
Page
702025, 70289
78
702968, 702824,
702005
70245, 702437
79
702813, 702818,
702825, 702077
702287.1, 702288.1,
702026, 702027
80
70201HP, 702885,
702892, 702888,
702075, 702076,
702891, 702014
702813, 702818,
702825, 702077
70288, 702823,
70256
84
702714, 702713,
702712, 702709
702813, 702818,
702825, 702077
702710.1, 702717.2
87
702288.1, 702027
80
702293,
702284,
702078,
702007,
IC:
N9 screw
104
702009
info@mn-net.com
Autosampler compatibility
PerkinElmer
Application/Type of vial
GC:
Vials:
Inserts:
Closures:
70252.1, 70283,
702025
78
702282, 702293,
702283, 702284,
702078, 702079
702818, 702825
702732, 702287.1,
702026, 702028
80
702012, 702013
702818, 702825
702045, 702046
83
N8 crimp (microsampling)
70251, 70286
702818**, 702825**
70256, 702730,
70231.1, 70231.2,
70231.3, 70288,
702995
Page
84
84
N8 crimp (microsampling)
70286
70252.1, 702025
78
As indicated under GC, but additionally the following closures with slit
septum: 702288.1, 702027
80
As indicated under GC, but additionally the following closure with slit
septum: 702823
84
702064, 702718,
702710, 702401
87
702866, 702826
702055, 702827,
702072
95
702924, 702263
702929, 702834,
702928.9, 702928,
702774
96
N20 crimp
(TurboMatrix HS 16, 40 + 110)
*** not suited for TurboMatrix110
702917***, 70254,
702540, 702541
702829, 702836,
702071, 70234.8,
702835, 702927,
702775, 702773,
70234, 70234.9,
702093, 702094,
70237, 702931
96
As indicated under GC, but additionally the following closure with slit
septum: 702047
702714, 702713,
702712
702818, 702825
83
Headspace:
N18 screw (CTC Combi PAL +
TurboMatrix HS 16 + 40)
www.mn-net.com
HPLC:
105
Autosampler compatibility
Shimadzu
Application/Type of vial
GC:
Vials:
N8 crimp (microsampling)
70282, 70286,
70212, 70212.1,
702002, 702003
Page
Inserts:
Closures:
70289, 702878
78
702282,
702283,
702006,
702079,
702008
702293,
702284,
702078,
702007,
702813, 702818,
702825, 702077
702287.1, 702035,
702026
80
702011, 702012,
702013
702813, 702818,
702825, 702077
702045, 702046,
702048
83
70201HP, 702885,
702892, 702888,
702075, 702076,
702891, 702014
702813, 702818,
702825, 702077
702879 (for
AOC5000), 70288,
702995
84
702962, 702973,
702089
702972 + spring
702974
702926
90
702025, 70289,
702878
78
HPLC:
N8 crimp (microsampling)
70282, 70286
702282,
702283,
702006,
702079,
702007,
702293,
702284,
702078,
702009,
702008
702813, 702818,
702825, 702077
702287.1, 702037,
702038, 702036,
702026,
702288.1, 702040,
702083, 702039,
702027, 702031
80
702011, 702012,
702013
702813, 702818,
702825, 702077
702045, 702046,
702047
83
70201HP, 702885,
702892, 702888,
702141, 702075,
702076, 702891,
702014
702813, 702818,
702825, 702077
702730, 70288,
702823
84
702714, 702713,
702712, 702809
702813, 702818,
702825, 702077
702064, 702717.2,
702710, 702710.1
87
Vials + closures:
70202.1 + 702807,
702017 + 702807,
702018 + 702054
94
702866, 702826
702055, 702827
702924, 702263
702929, 702834,
702928, 702774
95
96
702918, 702263
702094, 702093
96
Headspace:
N18 screw (AOC 5000)
Thermo Scientific
Application/Type of vial
GC:
Vials:
70251, 70282,
70286, 702880,
70212, 70212.1,
702002, 702003
N8 crimp (microsampling)
106
Inserts:
Closures:
70252.1, 702025,
70289, 702878
info@mn-net.com
Page
78
Autosampler compatibility
Application/Type of vial
Page
70213, 70213.2,
702004, 702893,
702860
702968, 702968.1,
702824, 702005
702067, 70245,
702069
702282,
702283,
702006,
702079,
702008
702813, 702818,
702716, 702825,
702077
702732, 702287.1,
702035, 702026
80
70201HP, 702885,
702892, 702888,
702141, 702075,
702076, 702891,
702014
702813, 702818,
702716, 702825,
702077
84
70252.1, 702025,
70289, 702878
78
702293,
702284,
702078,
702007,
79
HPLC:
70282, 70286,
702880, 70212,
70212.1, 702002,
702003
As indicated under GC
79
As indicated under GC
80
84
702866, 702826
702055, 702827
702924, 702263
702929, 702834
95
702924, 702263
702094, 702093,
702773/702775/
70234.9
702714, 702713,
702712, 702809
702813, 702818,
702716, 702825,
702077
702064, 702717.2,
702710, 702710.1,
702731, 702063
87
Headspace:
N18 screw (Combi PAL)
N20 crimp (Combi PAL)
96
96
GC:
Vials:
Inserts:
Closures:
70289, 702878
78
70213, 70213.2,
702004, 702893,
702860
702968, 702824
702067, 70245,
702069
79
702282, 702293,
702283, 702284,
702006, 702078,
702079
702813, 702818,
702716, 702825,
702077
702732, 702287.1,
702037, 702035,
702026
80
70201HP, 702885,
702892, 702888,
702075, 702076
702813, 702818,
702716, 702825,
702077
84
HPLC:
Vials:
Inserts:
Closures:
N8 crimp (microsampling)
N8 crimp (microsampling)
www.mn-net.com
70282, 70286,
702880, 70212,
70212.1, 702002,
702003
Page
N8 crimp (microsampling)
78
79
80
107
Autosampler compatibility
Varian (now Agilent)
Application/Type of vial
702813, 702818,
702716, 702825,
702077
702045/702046,
702048, 702044
Page
83
84
702866, 702826
702055, 702827,
702073
95
702924, 702263
702929, 702834
702924, 702918,
702261
702093, 70234,
702773/702775
96
702714, 702713,
702712, 702809
702813, 702818,
702716, 702825,
702077
Headspace:
702064, 702717.2,
702710, 702710.1,
702731, 702063,
702718
87
96
VWR (Merck/Hitachi)
Application/Type of vial
HPLC:
Page
Vials:
Inserts:
Closures:
70213, 70213.2,
702004, 702893,
702860
702968, 702968.1,
702824, 702005
702067, 70245,
702437
79
702282, 702293,
702283, 702284,
702078, 702079
702813, 702716,
702818, 702077
702287.1, 702288.1,
702026, 702027,
702031
80
702714, 702713,
702712
702813, 702716,
702818, 702077
702063, 702710.1,
702717.2
87
702962, 702973
702972 + spring
702974
702926, 702963 +
70260
90
N8 crimp (microsampling)
70286, 70282
70289, 702878
78
Waters
Application/Type of vial
HPLC:
Closures:
Page
702282,
702283,
702006,
702008,
702079,
702293,
702284,
702007,
702078,
702009
702818
702026, 702027,
702287.1, 702288.1,
702037, 702040,
702038, 702083,
702287, 702288
80
702011, 702012,
702013
702818
702045, 702046,
702047
83
702714, 702713,
702712, 702809,
702709
702818
702710.1, 702717.2
87
Vials + closures:
70202.1 + 702807,
702017 + 702807
94
702972 + spring
702974
702926,
702963 + 70260
90
108
Inserts:
702962, 702973,
702089
info@mn-net.com
HPLC Contents
Columns for HPLC
110
111
112
116
144
148
154
155
157
166
Summary
Analytical and preparative columns for special separations
167
168
189
196
197
198
199
201
203
204
www.mn-net.com
Liquid chromatography
109
In RP liquid chromatography the efficiency of the packing is strongly affected by the quality of the base silica itself. Shortcomings in the surface geometry of the
particles or metal contaminants are the main reasons
for inadequate coverage with the covalently bonded alkylsilanes in the subsequent derivatization steps. It is
well known, that poor surface coverage and, in consequence, high activity of residual free silanols often
results in peak tailing or adsorption, particularly with
basic compounds.
<
<
<
<
<
<
<
<
5
5
5
10
1
1
0.5
0.05
ppm
ppm
ppm
ppm
ppm
ppm
ppm
ppm
Pressure stability
The totally spherical and 100% synthetic silica gel exhibits an outstanding mechanical stability, even at high
pressures up to 800bar and elevated eluent flow rates.
In addition, after several cycles of repeated packing, no
significant drop in pressure can be observed. The latter is of prime importance for preparative and processscale applications.
Physical data of NUCLEODUR
Surface area (BET)
Pore size
Pore volume
340m2/g
110
0.9mL/g
NUCLEODUR modifications
Several different surface modifications based on
NUCLEODUR silica have been developed over the
last years providing a full range of specified HPLC
phases and an ideal tool for every separation:
NUCLEODUR silicas are synthesized in a unique and
carefully controlled manufacturing process which provides silica particles, which are totally spherical. The
picture shows the outstanding smoothness of the
NUCLEODUR surface.
Purity
As already mentioned above, a highly pure silica is required for achieving symmetric peak shapes and maximum resolution. Inclusions of, e.g., iron or alkaline
earth metal ions on the silica surface are largely responsible for the unwanted interactions with ionizable
analytes, e.g. amines or phenolic compounds.
110
info@mn-net.com
NUCLEODUR 1.8m particles are fractionated to limit the increase in back pressure.
Available in 1.8m: C18 Gravity, C8 Gravity, C18 Isis, C18 Pyramid, PolarTec, PFP, Sphinx RP, C18 HTec, HILIC
Increase of the plate number by ~67% offers the possibility of using shorter columns with equal plate number
resulting in a decrease of analysis time.
Significant improvement in resolution
N 1
Rs =
4
B)
1.8 m
Rs = 1.42
160 bar
DP = pressure drop
LC = column length
= viscosity
LC u
dP2
3m
1.8m
Competitor A
170bar
HETP [m]
DP =
ki
k i + 1
A)
3 m
Rs = 1.11
80 bar
Due to the smaller particles the back pressure will increase according to
Use of 1.8m instead of 3m particles leads to an increase of resolution by a factor of 1.29 (29%) since the
resolution is inversely proportional to the square root
of the particle size.
20
3 m
15
10
5
0
1.8 m
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
u [mm/s]
Technical requirements
0.4
0.6
www.mn-net.com
0.4
0.6 min
To gain best results with 1.8m particles certain technical demands must be met including pumps for flow
rates of 23mL with pressures of 2501000bar, minimized dead volume, and fast data recording
111
14% C USP L1
Bifunctional RP phase,
phenylpropyl and C18
ligands; endcapping
pH 110,
suitable for LC/MS
pH 19,
suitable for LC/MS
A
B
C
A
B
C
A
B
C
C
A
B
pH 110,
suitable for LC/MS
pH 111,
suitable for LC/MS
C
A
B
B
hydrophobic selectivity, B =
NUCLEODUR
(Si-O2)n
pH 111,
suitable for LC/MS
A
Pentafluorophenylpropyl modification with
B
multi-endcapping
8% C USP L43
112
NUCLEODUR
(Si-O2)n
NUCLEODUR
(Si-O2)n
20% C USP L1
NUCLEODUR
(Si-O2)n
pH 1-11,
suitable for LC/MS
NUCLEODUR
(Si-O2)n
11% C USP L7
NUCLEODUR
(Si-O2)n
Octyl phase
high density coating
multi-endcapping
Structure
NUCLEODUR
(Si-O2)n
Octadecyl phase,
high density coating,
multi-endcapping,
18% C USP L1
Characteristics* Stability
NUCLEODUR
(Si-O2)n
polar/ionic selectivity, C =
OH
OH
Pol
Si OH
Pol
Si O Si(CH3)3
Si OH
F
F
F F
O
Si
Si(CH3)3
steric selectivity
info@mn-net.com
Application
Similar phases**
NUCLEOSIL C18 HD
OH
Si(CH3)3
CH3
120
CH3
OH
H 3C
122
N
O
Hydrophobic
and polar
(H bonds)
Polar
ACQUITY CSH Fluoro-Phenyl;
(H bonds),
Compounds with
aromatic and multiple no similar phases
bond systems
Xterra RP18/MS C18/SunFire
C18; Luna C18(2), Gemini,
Synergi Max RP; Zorbax
Extend-C18; Inertsil ODSIII;
Purospher STAR RP-18;
Hypersil BDS
Pol
Si(CH3)3
HO
124
Pol
O
O
F
- and
hydrophobic
Hydrophobic
126
H
F
Hydrophobic
and polar
(H bonds)
CH3
N
OH O
116
Steric and
hydrophobic
Hydrophobic
Inertsil
ODS-P;
Pro C18 RS;
and structural iso
Zorbax
SB
mers, planar/nonplanar molecules
H 3C
CH3
Si(CH3)3
Page
NO2
128
CH3
Si(CH3)3
H 3C
N
O
130
** Phases which provide a similar selectivity based on chemical and physical properties
www.mn-net.com
113
Octyl phase,
medium density coating
endcapping
10.5% C USP L7
Zwitterionic ammonium
sulfonic acid phase
pH 19
C
A
B
C
A
B
pH 28.5,
suitable for LC/MS
Amino phase
for NP and RP
separations
2.5% C USP L8
Unmodified high purity
silica
USP L3
B
C
A
B
C
pH 18,
stable towards
highly aqueous
mobile phases
NUCLEODUR
(Si-O2)n
7% C USP L10
pH 28,
stable towards
highly aqueous
mobile phases
NUCLEODUR
(Si-O2)n
*A=
pH 19
pH 28
NUCLEODUR
(Si-O2)n
7% C
NUCLEODUR
(Si-O2)n
17.5% C USP L1
A
B
C
hydrophobic selectivity, B =
Structure
NUCLEODUR
(Si-O2)n
Octadecyl phase,
medium density coating
endcapping
Characteristics* Stability
NUCLEODUR
(Si-O2)n
polar/ionic selectivity, C =
Si OH
Si O Si(CH )
3 3
Si OH
Si O Si(CH )
3 3
+N
Si OH
+N
Si OH
CH3
CH3
CH3
CH3
SO3
SO3
C N
Si OH
C N
Si O Si(CH )
3 3
NH2
Si OH
NH2
Si OH
Si OH
Si OH
steric selectivity
114
info@mn-net.com
Similar phases**
NUCLEOSIL C18
H3C
SiOH
133
H 3C
Si(CH3)3
SiOH
N
CH3
Ionic/
hydrophilic
and
electrostatic
- and
polar
(H bonds),
hydrophobic
NUCLEOSIL NH2/NH2-RP
Polar/ionic
and
hydrophobic
Polar compounds in
general
NUCLEOSIL SiOH
H 3C
H 3C
CH3
N NH
O
HO
CH3
SO3
NH2
136
O
C
N
SO3
CH3
CH3
HO
138
NH3
OH
140
Polar/ionic
SiOH
O2N
** Phases which provide a similar selectivity based on chemical and physical properties
142
CH3
Si(CH3)3
Hydrophobic
Page
www.mn-net.com
115
Key features:
Suitable for LC/MS and HPLC at pH extremes (pH 111)
Superior base deactivation
Ideal for method development
Recommended application:
Overall sophisticated analytical separations
Compound classes separated include pharmaceuticals, e.g., analgesics, anti-inflammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosuppressants
USP L1 (C18)/USP L7 (C8)
Base deactivation
NUCLEODUR C18 Gravity and NUCLEODUR C8 Gravity are based on the ultrapure NUCLEODUR silica. Derivatization generates a homogeneous surface with a
high density of bonded silanes (~18% C for C18, ~11% C
for C8). Thorough endcapping suppresses any unwanted polar interactions between the silica surface and the
sample, which makes Gravity particularly suitable for
the separation of basic and other ionizable analytes.
Even strongly basic pharmaceuticals like amitriptyline
are eluted without tailing under isocratic conditions.
For a discussion of the different retention behavior of
C18 phases compared to C8 phases see page 134.
Enhanced pH stability
An expanded pH range is often required in method development. Many nitrogen containing compounds like
basic drugs are protonated at acidic or neutral pH and
exhibit poor retention on a standard C18 phase. The retention behavior can be improved by working at a higher pH, where the analyte is no longer protonated, but
formally neutrally charged, as a rule between pH 910.
For acidic analytes it is exactly in inverse proportion,
maximum retention can be attained at low pH.
116
Me
OH
Me
Pore size 110; particle sizes 1.8m, 3m and 5m for C18, 1.8 and 5m for C8;
7, 10, 12 and 16m particles for preparative purposes on request
OH
O
O
O Si
O
O
O Si
O
O Si
OOC
Me
OH
OH
MeHN
Me
Technical characteristics:
Available as octadecyl (C18) and octyl (C8), multi-endcapped
Me
OH
OH
O Si
OH
OH
O Si
OH
O Si
H+
NH2Me
HOOC
Me
H+
H+
H+
HA
k
BH+
0
pH
12
info@mn-net.com
CH3
NH
H 3C
N(C2H5)2
Peaks:
1. Lidocaine
2. Papaverine
3. Noscapine
4. Diphenhydramine
pH 2.5
NH2
pH 10.0
min
pH 3.0
2
OH
O
pH 10.0
H3C
The following chromatogram demonstrates the stability of NUCLEODUR C18 Gravity under alkaline conditions. The ultrapure Gravity with its unique high density
surface bonding technology withstands strong alkaline
mobile phase conditions.
Column:
Eluent:
1st injection
min
At pH 2.5 the protonated analytes exhibit poor retention (early elution) and in addition an inadequate resolution for papaverine and noscapine, whilst the formally
non ionized molecules can be baseline separated due
to the better retention pattern at alkaline pH.
www.mn-net.com
min
MN Appl. No. 120850
117
Under alkaline conditions dissolution of the silica support is possible, resulting in dead volume and thus
peak broadening. It is worth mentioning, that this phenomenon also depends on type and concentration of
buffers, as well as on the temperature. It is well known
that the use of phosphate buffers, particularly at elevated temperatures, can reduce column lifetime even
at moderate pH. If possible, phosphate buffers should
be replaced by less harmful alternatives.
Column:
125 x 4mm NUCLEODUR C18 Gravity, 5m
Eluent:
acetonitrile 1% TFA in water (50:50, v/v), pH 1.5
Flow rate: 1.0mL/min
Temperature: 30C,
Detection: UV, 230nm
Injection:
5L
Peaks: 1. Pyridine, 2. Toluene, 3. Ethylbenzene
The following chromatograms show the excellent column stability of NUCLEODUR C18 Gravity in acidic
conditions. Retention times of all three compounds in
the column performance test remain consistent and
virtually unchanged, even after the column is run with
5000mL eluent. Due to the extremely stable surface
modification, no cleavage of the Si-O-Si bonding occurs, column deterioration is therefore successfully
prevented.
1st injection
10
min
Ordering information
30mm
50mm
75mm
ID
ID
ID
ID
760078.20
760078.30
760078.40
760078.46
760079.20 760071.20
760079.30
760079.40
760079.46
4 x 2mm: 761901.20
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
760080.20
760080.30
760080.40
760080.46 760086.46
4 x 2mm: 761902.20
8 x 3mm: 761124.30
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
118
ID
ID
ID
ID
760102.20
760102.30
760102.40
760102.46 760106.46
4 x 2mm: 761903.20
8 x 3mm: 761125.30
100mm
125mm
150mm
250mm
760075.20
760083.20
760083.30
760083.40
760083.46
760082.20
760082.30
760082.40
760082.46
760100.20
760100.30
760100.40
760100.46
761903.30
761125.40
760103.20
760103.30
760103.40
760103.46
760101.20
760101.30
760101.40
760101.46
info@mn-net.com
VarioPrep columns
10mm
21mm
32mm
40mm
VP guard columns***
30mm
50mm
75mm
762103.100
762103.210
ID
ID
ID
ID
100mm
125mm
150mm
250mm
762109.100
762109.210
10 x 8mm: 762160.80
762113.100
762113.210
762113.320
762100.400 762113.400
10 x 16mm: 762160.160 15 x 32mm: 762163.320
VarioPrep columns
762250.210
762250.400
21mm ID
40mm ID
VP guard columns***
Analytical EC columns
2mm
3mm
4mm
4.6mm
EC guard columns*
ID
ID
ID
ID
760756.20
760756.30
760756.40
760756.46
760755.20 760760.20
760755.30
760755.40
760755.46
4 x 2mm: 761905.20
760757.20
760757.30
760757.40
760757.46
4 x 3mm: 761905.30
NUCLEODUR C8 Gravity, 5m
Analytical EC columns
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
VarioPrep columns
10mm ID
21mm ID
760759.20
760750.20
760750.30
760750.40
760750.46 760749.46
4 x 2mm: 761907.20
8 x 3mm: 761754.30
760754.20
760754.30
760754.40
760754.46
4 x 3mm:
8 x 4mm:
762081.100
762081.210
760751.20
760751.30
760751.40
760751.46
761907.30
761754.40
760752.20
760752.30
760752.40
760752.46
760753.20
760753.30
760753.40
760753.46
762071.100
762070.100
762071.210 762082.210 762070.210
VP guard columns***
10 x 8mm: 762097.80 10 x 16mm: 762097.160
EC and VarioPrep columns in packs of 1 column, guard columns see below
EC
CC
VP
2mm
3mm
4mm
4.6mm
4/2 (3)
8/3 (3)
4/3 (3)
8/3 (3)
4/3 (3)
8/4 (3)
4/3 (3)
8/4 (3)
8, 10mm
16, 21mm
32, 40mm
50mm
10/8 (2)
718251
10/16 (2)
718256
15/32 (1)
718253
15/50 (1)
718255
www.mn-net.com
119
Key features:
Exceptional steric selectivity
Outstanding surface deactivation
Suitable for LC/MS and HPLC at pH 110
Technical characteristics:
C18 phase with special polymeric, crosslinked surface modification; pore size 110;
particle sizes 1.8m, 3m and 5m; carbon content 20%
Recommended application:
Steroids, (o,p,m-) substituted aromatics, fat-soluble vitamins
USP L1
Surface modification
By use of specific C18 silanes and polymeric bonding technologies a dense shield of alkyl chains protects the subjacent silica matrix. Elemental analysis of
NUCLEODUR C18 Isis shows a carbon load of 20%. The
target crosslinking of the C18 chains on the surface enables the separation of compounds with similar molecular structure but different stereochemical properties.
The technical term for this feature is steric selectivity.
Slot model
Sander and Wise [LCGC 8 (1990) 378390] proposed a model for the retention of aromatic compounds based on
molecular shape, which is referred to as Slot Model.
This model pictures the bonded C18 phase on the silica
surface with slots which the analytes have to penetrate
during retention. Planar molecules are able to penetrate
these slots deeper than non-planar molecules of similar molecular weight and length-to-breadth ratio. Thus
triphenylene is longer retained than o-terphenyl.
min
Columns:
125 x 4mm
Eluent:
methanol water (80:20, v/v)
Flow rate: 1mL/min, temperature 40C
Detection: UV, 254nm, injection 1L
Peaks: 1. o-Terphenyl, 2. Triphenylene
monomerically
coated C18 phase
NUCLEODUR
C18 Isis
1
2
Steric selectivity
120
= 1.35
0
= 1.93
5
10
10 min
Ordering information
30mm
50mm
75mm
100mm
125mm
ID
ID
ID
ID
760406.20
760406.30
760406.40
760406.46
760405.20 760396.20
760405.30
760405.40
760405.46
4 x 2mm: 761910.20
760407.20
760407.30
760407.40
760407.46
4 x 3mm: 761910.30
ID
ID
ID
ID
particle size 3m
760400.20
760400.30
760400.40
760400.46 760397.46
4 x 2mm: 761911.20
8 x 3mm: 761300.30
760401.20
760401.30
760401.40
760401.46
4 x 3mm:
8 x 4mm:
760402.20
760402.30
760402.40
760402.46
761911.30
761300.40
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
VarioPrep columns
760409.20
760403.20
760403.30
760403.40
760403.46
760404.20
760404.30
760404.40
760404.46
particle size 5m
760410.20
760410.30
760410.40
760410.46 760416.46
4 x 2mm: 761912.20
8 x 3mm: 761310.30
760415.20
760415.30
760415.40
760415.46
4 x 3mm:
8 x 4mm:
760412.20
760412.30
760412.40
760412.46
761912.30
761310.40
760413.20
760413.30
760413.40
760413.46
760414.20
760414.30
760414.40
760414.46
10mm ID
762404.100
762405.100
762403.100
21mm ID
762404.210
762405.210
762403.210
32mm ID
762403.320
40mm ID
762406.400 762403.400
VP guard columns***
10 x 8mm: 762420.80 10 x 16mm: 762420.160 15 x 32mm: 762422.320
EC and VarioPrep columns in packs of 1 column, guard columns see below
EC
CC
VP
2mm
3mm
4mm
4.6mm
4/2 (3)
8/3 (3)
4/3 (3)
8/3 (3)
4/3 (3)
8/4 (3)
4/3 (3)
8/4 (3)
8, 10mm
16, 21mm
32, 40mm
50mm
10/8 (2)
718251
10/16 (2)
718256
15/32 (1)
718253
15/50 (1)
718255
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
250mm
150mm
www.mn-net.com
121
Key features:
Stable in 100% aqueous mobile phase systems
Interesting polar selectivity features
Excellent base deactivation; suitable for LC/MS due to low
bleeding characteristics
Technical characteristics:
Special phase with polar endcapping; pore size 110; particle
sizes 1.8m, 3m and 5m (7 and 10m particles for preparative purposes on request); carbon content 14%; pH stability 19
Recommended application:
Analgesics, penicillin antibiotics, nucleic acid bases, water-soluble
vitamins, complexing agents, organic acids
USP L1
Stability features
NUCLEODUR C18 Pyramid is a silica phase with hydrophilic endcapping, designed especially for use in eluent
systems of up to 100% water. The upper figure shows
the retention behavior of tartaric, acetic and maleic acid
under purely aqueous conditions on NUCLEODUR C18
Pyramid in comparison with a conventionally bonded
C18 phase. It can be shown that the retention times for
NUCLEODUR C18 Pyramid remain nearly unchanged
between initial injection and restart after the flow has
been stopped for 12h, whilst the performance of the
conventional RP column already collapsed totally 5min.
122
Stability test
Initial injection
C18 Pyramid
Conventional C18
Restart after 12 h
Stop
3
2
Stop
5 min
5 min
Retention characteristics
Separation of very polar compounds
Column:
125 x 4mm NUCLEODUR C18 Pyramid, 5m
Eluent:
0.2% H3PO4
1
Flow rate: 1.0mL/min
Temperature: 22C
2
Detection: UV, 202nm
Injection:
2L
Peaks:
1. Formic acid
2. Acetic acid
t0
info@mn-net.com
min
tion No. 119190 at www.mn-net.com). The perceptible increase in polarity has no impact on the retention
behavior of ionizable analytes. Even with the strongly
basic compounds of the tricyclic antidepressant drug
test mixture, no unwanted interactions or a so-called
lack in base deactivation are observed (see application
119200 at www.mn-net.com/apps).
Ordering information
30mm
50mm
75mm
100mm
125mm
760271.20
760271.30
760271.40
760271.46
760272.20 760275.20
760272.30
760272.40
760272.46
4 x 2mm: 761915.20
760273.20
760273.30
760273.40
760273.46
4 x 3mm: 761915.30
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
VarioPrep columns
760274.20
particle size 3m
760263.20
760263.30
760263.40
760263.46 760259.46
4 x 2mm: 761916.20
8 x 3mm: 761854.30
760264.20
760264.30
760264.40
760264.46
4 x 3mm:
8 x 4mm:
760260.20
760260.30
760260.40
760260.46
761916.30
761854.40
250mm
2mm
3mm
4mm
4.6mm
EC guard columns*
150mm
760261.20
760261.30
760261.40
760261.46
760262.20
760262.30
760262.40
760262.46
particle size 5m
760200.20
760200.30
760200.40
760200.46 760205.46
4 x 2mm: 761917.20
8 x 3mm: 761800.30
760204.20
760204.30
760204.40
760204.46
4 x 3mm:
8 x 4mm:
760201.20
760201.30
760201.40
760201.46
761917.30
761800.40
760203.20
760203.30
760203.40
760203.46
760202.20
760202.30
760202.40
760202.46
10mm ID
762271.100
762273.100
762272.100
21mm ID
762271.210
762273.210
762272.210
32mm ID
762272.320
40mm ID
762269.400 762272.400
VP guard columns***
10 x 8mm: 762291.80 10 x 16mm: 762291.160 15 x 32mm: 762293.320
EC and VarioPrep columns in packs of 1, guard columns see below; details of our column systems see pages 189196
EC
CC
VP
www.mn-net.com
2mm
3mm
4mm
4.6mm
4/2 (3)
8/3 (3)
4/3 (3)
8/3 (3)
4/3 (3)
8/4 (3)
4/3 (3)
8/4 (3)
8, 10mm
16, 21mm
32, 40mm
50mm
10/8 (2)
718251
10/16 (2)
718256
15/32 (1)
718253
15/50 (1)
718255
123
Key features:
Excellent base deactivation
Suitable for LC/MS and 100% aqueous eluents
Pronounced steric selectivity
Technical characteristics:
Phase with embedded polar group; pore size 110; particle sizes 1.8m,
3m and 5m; carbon content 17%; pH stability 19
Recommended application:
Exceptional selectivity for phenols and nitrogen containing compounds,
polar compounds like basic pharmaceuticals, organic acids, pesticides,
amino acids, water-soluble vitamins, etc.
USP L1 and L60
R1
OH
R2
Even after days or weeks of operation in purely aqueous eluents the C18 chains of NUCLEODUR PolarTec are
neither folded nor show any collapsing. A significant
reduction of retention time cannot be observed.
Stability of NUCLEODUR PolarTec
Column:
150 x 3mm NUCLEODUR PolarTec, 3m
Eluent:
30mmol/L KH2PO4, pH 3.0
Flow rate: 0.5mL/min
Temperature: 30C
Detection: UV, 220nm
Peaks:
1. Cytosine
1
2. Uracil
3. Adenine
4. Guanine
5. Thymine
min
2 3
4
198 h
0h
0
7 min
MN Appl. No. 124610
124
info@mn-net.com
hydrophobic properties and is very well suited for analyzing basic compounds.
Ordering information
30mm
50mm
75mm
100mm
125mm
ID
ID
ID
ID
760461.20
760461.30
760461.40
760461.46
760463.20 760465.20
760463.30
760463.40
760463.46
4 x 2mm: 761980.20
760466.20
760466.30
760466.40
760466.46
4 x 3mm: 761980.30
ID
ID
ID
ID
particle size 3m
760473.20
760473.30
760473.40
760473.46 760475.46
4 x 2mm: 761981.20
8 x 3mm: 761160.30
760476.20
760476.30
760476.40
760476.46
4 x 3mm:
8 x 4mm:
760477.20
760477.30
760477.40
760477.46
761981.30
761160.40
NUCLEODUR PolarTec, 5m
Analytical EC columns
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
VarioPrep columns
10mm
21mm
32mm
40mm
VP guard columns***
ID
ID
ID
ID
ID
ID
ID
ID
760468.20
760478.20
760478.30
760478.40
760478.46
particle size 5m
760483.20
760483.30
760483.40
760483.46 760485.46
4 x 2mm: 761982.20
8 x 3mm: 761161.30
762220.100
762220.210
760486.20
760486.30
760486.40
760486.46
4 x 3mm:
8 x 4mm:
760487.20
760487.30
760487.40
760487.46
761982.30
761161.40
760488.20
760488.30
760488.40
760488.46
762223.100
762223.210
762223.320
762222.400 762223.400
10 x 16mm: 762224.160 15 x 32mm: 762226.320
EC
CC
VP
760489.20
760489.30
760489.40
760489.46
762221.100
762221.210
10 x 8mm: 762224.80
760479.20
760479.30
760479.40
760479.46
2mm
3mm
4mm
4.6mm
4/2 (3)
8/3 (3)
4/3 (3)
8/3 (3)
4/3 (3)
8/4 (3)
4/3 (3)
8/4 (3)
8, 10mm
16, 21mm
32, 40mm
50mm
10/8 (2)
718251
10/16 (2)
718256
15/32 (1)
718253
15/50 (1)
718255
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
250mm
NUCLEODUR PolarTec, 3m
Analytical EC columns
150mm
www.mn-net.com
125
Recommended application:
Aromatic and unsaturated compounds, phenols, halogen compounds, isomers, polar compounds like pharmaceuticals, antibiotics; strong retention
of basic compounds
USP L43
Orthogonality in selectivity
Columns:
23
NUCLEODUR PFP offers four different retention mechanisms: polar interactions (H bonds), dipole-dipole,
-, and hydrophobic interactions. Especially the pronounced ion exchange capacity and distinct steric selectivity are typical for fluorinated phases. Due to low
bleeding characteristics NUCLEODUR PFP is also suitable for LC/MS. Based on a special surface modification
procedure NUCLEODUR PFP offers highest stability
also at low pH values.
Eluent:
Flow rate:
Detection:
Peaks:
1. o-Cresol
2. m-Cresol
3. 3,4-Dimethylphenol
4. 3,5-Dimethylphenol
5. 2,5-Dimethylphenol
6. 2,6-Dichlorophenol
7. 2,3-Dichlorophenol
8. 2,4-Dichlorophenol
9. 3,4-Dichlorophenol
10. 2,4-Dibromophenol
11. 3,5-Dibromophenol
6
7
1 2
8
9
10
8
1
0
2 34
5
6
10
8
15
7
20
min
While a typical C18 phase just provides hydrophobic interactions between stationary phase and analyte
126
11
12
0
34 5
6 7 8 9
6
10 11
10
12
14 min
info@mn-net.com
Ordering information
30mm
50mm
75mm
100mm
125mm
ID
ID
ID
ID
760431.20
760431.30
760431.40
760431.46
760433.20 760435.20
760433.30
760433.40
760433.46
4 x 2mm: 761975.20
760436.20
760436.30
760436.40
760436.46
4 x 3mm: 761975.30
ID
ID
ID
ID
particle size 3m
760443.20
760443.30
760443.40
760443.46 760445.46
4 x 2mm: 761976.20
8 x 3mm: 761145.30
760446.20
760446.30
760446.40
760446.46
4 x 3mm:
8 x 4mm:
760447.20
760447.30
760447.40
760447.46
761976.30
761145.40
NUCLEODUR PFP, 5m
Analytical EC columns
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
VarioPrep columns
10mm
21mm
32mm
40mm
VP guard columns***
ID
ID
ID
ID
ID
ID
ID
ID
760438.20
760448.20
760448.30
760448.40
760448.46
760449.20
760449.30
760449.40
760449.46
particle size 5m
760453.20
760453.30
760453.40
760453.46 760455.46
4 x 2mm: 761977.20
8 x 3mm: 761146.30
762210.100
762210.210
760456.20
760456.30
760456.40
760456.46
4 x 3mm:
8 x 4mm:
760457.20
760457.30
760457.40
760457.46
761977.30
761146.40
760458.20
760458.30
760458.40
760458.46
762211.100
762211.210
10 x 8mm: 762214.80
762213.100
762213.210
762213.320
762212.400 762213.400
10 x 16mm: 762214.160 15 x 32mm: 762216.320
EC
CC
VP
760459.20
760459.30
760459.40
760459.46
2mm
3mm
4mm
4.6mm
4/2 (3)
8/3 (3)
4/3 (3)
8/3 (3)
4/3 (3)
8/4 (3)
4/3 (3)
8/4 (3)
8, 10mm
16, 21mm
32, 40mm
50mm
10/8 (2)
718251
10/16 (2)
718256
15/32 (1)
718253
15/50 (1)
718255
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
250mm
NUCLEODUR PFP, 3m
Analytical EC columns
150mm
www.mn-net.com
127
bifunctional RP phase
Key features:
Distinct selectivity based on well-balanced bifunctional surface coverage
Widens the scope for method development based on additional - interactions
Suitable for LC/MS due to low bleeding characteristics
Technical characteristics:
Octadecyl and propylphenyl modified silica; pore size 110; particle sizes
1.8m, 3m and 5m; carbon content 15%;
pH stability 110; high reproducibility and consistent quality
Recommended application:
Quinolone antibiotics, sulfonamides, xanthines, substituted aromatics
USP L1 and L11
Alternative RP selectivity
Column:
Eluent:
Flow rate:
Detection:
1
Columns:
150 x 4.6mm
NUCLEODUR Sphinx RP, 5m
NUCLEODUR C18 Gravity, 5m
NUCLEODUR C8 Gravity, 5m
Eluent:
water methanol (40:60, v/v)
Flow rate:
1mL/min
Temperature: 30C
Detection: UV, 270nm
Injection:
3L
OH
1
Peaks:
OH
HO
R1
OH
HO
OH
OH
1. Catechin
2. Rutin
3. Fisetin
4. Quercetin
5. Kaempferol
6. Isorhamnetin
26
R3 O
R2
R1 = R3 = OH, R2 = O-Rutinose
R1 = R2 = OH, R3 = H
R1 = R2 = R3 = OH
R1 = H, R2 = R3 = OH
R1 = OCH3, R2 = R3 = OH
2
3
Sphinx RP
1st injection
0
C18 Gravity
min
128
C8 Gravity
0.0
2.5
5.0
7.5 min
MN Appl. No. 119830
info@mn-net.com
30mm
50mm
75mm
100mm
125mm
ID
ID
ID
ID
760821.20
760821.30
760821.40
760821.46
760822.20 760825.20
760822.30
760822.40
760822.46
4 x 2mm: 761920.20
760823.20
760823.30
760823.40
760823.46
4 x 3mm: 761920.30
ID
ID
ID
ID
particle size 3m
760806.20
760806.30
760806.40
760806.46 760813.46
4 x 2mm: 761921.20
8 x 3mm: 761557.30
760812.20
760812.30
760812.40
760812.46
4 x 3mm:
8 x 4mm:
760807.20
760807.30
760807.40
760807.46
761921.30
761557.40
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
VarioPrep columns
10mm
21mm
32mm
40mm
VP guard columns***
ID
ID
ID
ID
ID
ID
ID
ID
760824.20
760805.20
760805.30
760805.40
760805.46
particle size 5m
760800.20
760800.30
760800.40
760800.46 760815.46
4 x 2mm: 761922.20
8 x 3mm: 761550.30
762372.100
762372.210
760809.20
760809.30
760809.40
760809.46
4 x 3mm:
8 x 4mm:
760801.20
760801.30
760801.40
760801.46
761922.30
761550.40
760802.20
760802.30
760802.40
760802.46
762373.100
762373.210
762373.320
762371.400 762373.400
10 x 16mm: 762390.160 15 x 32mm: 762392.320
EC
CC
VP
760803.20
760803.30
760803.40
760803.46
762375.100
762375.210
10 x 8mm: 762390.80
760808.20
760808.30
760808.40
760808.46
2mm
3mm
4mm
4.6mm
4/2 (3)
8/3 (3)
4/3 (3)
8/3 (3)
4/3 (3)
8/4 (3)
4/3 (3)
8/4 (3)
8, 10mm
16, 21mm
32, 40mm
50mm
10/8 (2)
718251
10/16 (2)
718256
15/32 (1)
718253
15/50 (1)
718255
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
250mm
150mm
www.mn-net.com
129
Key features:
Reliable and durable standard RP phase for up-scaling to preparative scale,
suited for LC/MS
High loading capacity and excellent stability
Outstanding base deactivation
Technical characteristics:
High density octadecyl modification (C18); pore size 110;
particle sizes 1.8m, 3m, 5m, 7m and 10m for analytical and preparative
separations; carbon content 18%, pH stability 111
Recommended application:
Sophisticated analytical and preparative separations of basic, neutral and acidic
pharmaceuticals, derivatized amino acids, pesticides, fat-soluble vitamins, aldehydes, ketones and phenolic compounds
USP L1
The innovative endcapping procedure leads to exceptionally good base deactivation the Engelhardt test
demonstrates superb selectivity, peak symmetry and
peak shape over the entire polarity range. In addition
NUCLEODUR C18 HTec scores in low bleed characteristics and is therefore highly suitable for LC/MS.
Engelhardt test
Column:
150 x 4mm NUCLEODUR C18 HTec, 5m
Flow rate:
1mL/min
Detection:
UV, 254nm
Injection:
5L
pH 10:
pH 1:
Eluent: methanol 50mmol/L
Eluent: acetonitrile
triethylamine
1% TFA in water
(25:85, v/v); 50C
(50:50, v/v); 80C
% initial retention of
% initial N of
ethylbenzene
theophylline
693 injections
1034 injections
Column:
250 x 4mm NUCLEODUR C18 HTec, 5m
Eluent:
methanol water (49:51, v/v)
Flow rate:
1mL/min
Temperature: 40C
Detection:
UV, 254nm
Injection:
5L
Peaks:
1. Uracil
2
4
2. Aniline
3. Phenol
4. p-Ethylaniline
5. N,N-Dimethylaniline
6. Toluene
7. Ethylbenzene
3
5
%
100
80
60
40
20
10
20
30
40
min
20
40
60
80 h
40
80
120
160
200 h
Due to innovative surface coating procedures NUCLEODUR C18 HTec offers excellent analytical separation properties and is the first choice for up-scaling to preparative column dimensions.
130
info@mn-net.com
2
1
3
min
min
Ordering information
30mm
50mm
75mm
100mm
125mm
ID
ID
ID
ID
760301.20
760301.30
760301.40
760301.46
760305.20 760304.20
760305.30
760305.40
760305.46
4 x 2mm: 761925.20
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
www.mn-net.com
760321.20
760321.30
760321.40
760321.46 760322.46
4 x 2mm: 761926.20
8 x 3mm: 761120.30
250mm
150mm
Columns:
Capacity
760308.20
particle size 3m
760323.20
760323.30
760323.40
760323.46
4 x 3mm:
8 x 4mm:
760324.20
760324.30
760324.40
760324.46
761926.30
761120.40
760325.20
760325.30
760325.40
760325.46
760326.20
760326.30
760326.40
760326.46
131
30mm
50mm
75mm
100mm
125mm
150mm
Analytical EC columns
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
10mm
21mm
32mm
40mm
50mm
VP guard columns***
ID
ID
ID
ID
ID
particle size 5m
760311.20
760311.30
760311.40
760311.46 760312.46
4 x 2mm: 761927.20
8 x 3mm: 761110.30
760313.20
760313.30
760313.40
760313.46
4 x 3mm:
8 x 4mm:
760314.20
760314.30
760314.40
760314.46
761927.30
761110.40
10mm
21mm
32mm
40mm
50mm
VP guard columns***
ID
ID
ID
ID
ID
762554.100
762553.210 762554.210
762553.320
762555.320
762555.400
762553.500
762555.500
10 x 8mm: 762591.80 10 x 16mm: 762591.160
15 x 32mm: 762592.320 15 x 50mm: 762592.500
10mm
21mm
32mm
40mm
50mm
VP guard columns***
ID
ID
ID
ID
ID
760316.20
760316.30
760316.40
760316.46
762556.100
762556.210
762556.320
762556.400
762556.500
particle size 7m
762561.100
762561.210
762564.100
762563.210 762564.210
762563.320
762565.320
762565.400
762563.500
762565.500
10 x 8mm: 762591.80 10 x 16mm: 762591.160
15 x 32mm: 762592.320 15 x 50mm: 762592.500
760315.20
760315.30
760315.40
760315.46
762551.100
762551.210
250mm
762566.100
762566.210
762566.320
762566.400
762566.500
762571.100
762571.210
762574.100
762573.210 762574.210
762573.320
762575.320
762575.400
762573.500
762575.500
10 x 8mm: 762591.80 10 x 16mm: 762591.160
15 x 32mm: 762592.320 15 x 50mm: 762592.500
762576.100
762576.210
762576.320
762576.400
762576.500
EC
CC
VP
2mm
3mm
4mm
4.6mm
4/2 (3)
8/3 (3)
4/3 (3)
8/3 (3)
4/3 (3)
8/4 (3)
4/3 (3)
8/4 (3)
8, 10mm
16, 21mm
32, 40mm
50mm
10/8 (2)
718251
10/16 (2)
718256
15/32 (1)
718253
15/50 (1)
718255
132
info@mn-net.com
Key features:
Ideal and reliable standard RP phase for daily routine analysis and up-scaling for
preparative HPLC
Medium density octadecyl (C ) and octyl (C ) modification with exhaustive end18
8
capping
Wide range of application areas
Recommended application:
Basic, neutral or acidic drugs, derivatized amino acids, pesticides
fat-soluble vitamins, aldehydes and ketones, phenolic compounds
USP L1 (C18)/L7 (C8)
Loading capacity
Loading capacity, probably the most important feature for preparative LC applications, is determined by
pore size, pore volume and surface area of the packing. However, it can also be influenced by the molecular
weight of the analytes. In the figure below the mass
loading curve for acetophenone and butyrophenone on
a NUCLEODUR 100-20 C18 ec column describes the
correlation between the increase of column loading and
the decrease of separation efficiency.
1000 Inj.
750 Inj.
500 Inj.
3
2
250 Inj.
Start
3 min
Loading curve
1000 Inj.
theoret. plates
2000
butyrophenone
0
10
100
g / compound
www.mn-net.com
5 min
Chemical stability
acetophenone
2000
0
500 Inj.
0
3000
Technical characteristics:
Pore size 110; particle sizes 3m and 5m; 7m, 10m, 12m, 16m, 20m,
30m and 50m for preparative separations; carbon content 17.5% for C18,
10.5% for C8
1000
10000
133
In addition to NUCLEODUR C18 phases MACHEREYNAGEL offers octyl modified NUCLEODUR C8 Gravity
and NUCLEODUR C8 ec columns to expand the RP tool
box. Based on the same spherical high purity silica the
C8 phases exhibit the same chemical and mechanical
stability as the C18 counterparts. Indeed NUCLEODUR
C8 Gravity can also be run at pH extremes (pH 111)
by choosing appropriate elution parameters. Due to the
shorter chain and less hydrophobic properties of the
stationary phase the retention of non-polar compounds
is decreased, and in consequence a reduction in time
of analysis can be achieved. Moreover a stronger polar
selectivity, particularly with the separation of ionizable
analytes is frequently observed (as distinct from the
C18 phases). NUCLEODUR C8 ec and NUCLEODUR C8
Gravity are most suitable for the development of new
methods but also for robust routine analyses.
Separation of phenols
5
2
4
2
C8 ec
5
89
C18 ec
10
15
min
High density C8 and C18 phases allow tailing-free elution, also for very polar compounds.
Octyl phases (C8) show superior polar selectivity.
Ordering information
50mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
760050.20
760050.30
760050.40
760050.46
75mm
100mm
125mm
150mm
250mm
760051.20
760051.30
760051.40
760051.46
4 x 3mm:
8 x 4mm:
760053.20
760053.30
760053.40
760053.46
761931.30
761005.40
760052.20
760052.30
760052.40
760052.46
134
info@mn-net.com
50mm
Analytical EC columns
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
VarioPrep columns
10mm
21mm
32mm
40mm
VP guard columns***
ID
ID
ID
ID
760004.20
760004.30
760004.40
760004.46
ID 762003.100
ID 762003.210
ID
ID
VarioPrep columns
10mm
21mm
32mm
40mm
50mm
VP guard columns***
ID 762011.100
ID 762011.210
ID
ID
ID
100mm
125mm
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
760063.20
760063.30
760063.40
760063.46
NUCLEODUR 100-5 C8 ec
Analytical EC columns
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
VarioPrep columns
10mm
21mm
32mm
40mm
VP guard columns***
ID
ID
ID
ID
760700.20
760700.30
760700.40
760700.46
760013.20
760013.30
760013.40
760035.46
760013.46
4 x 2mm: 761932.20
8 x 3mm: 761100.30
760001.20
760001.30
760001.40
760001.46
4 x 3mm:
8 x 4mm:
760008.20
760008.30
760008.40
760008.46
761932.30
761100.40
762029.100
762029.210
10 x 8mm: 762090.80
15 x 32mm: 762311.320
762027.400
10 x 16mm: 762090.160
15 x 50mm: 762311.500
760002.20
760002.30
760002.40
760002.46
762022.100
762022.210
762022.320
762022.400
10 x 16mm: 762090.160
15 x 50mm: 762311.500
762010.100
762010.210
762010.320
762010.400
762010.500
760060.20
760060.30
760060.40
760060.46
760061.46
4 x 3mm: 761936.30
8 x 4mm: 761012.40
760062.20
760062.30
760062.40
760062.46
ID 762072.100
ID 762072.210
ID
ID
10 x 8mm: 762092.80
760701.20
760701.30
760701.40
760701.46
760702.46
4 x 3mm: 761937.30
8 x 4mm: 761704.40
762061.100
762061.210
10 x 16mm: 762092.160
EC and VarioPrep columns in packs of 1, guard columns see previous NUCLEODUR phases
www.mn-net.com
250mm
NUCLEODUR 100-3 C8 ec
Analytical EC columns
150mm
75mm
760703.20
760703.30
760703.40
760703.46
762062.100
762062.210
762062.320
762079.400
762062.400
15 x 32mm: 762321.320
135
HILIC
te
aly
an
NPC
ad
so
rbe
nt
NUCLEODUR HILIC
zwitterionic phase
Key features:
Ideal for reproducible and stable chromatography of highly polar
analytes
Suitable for analytical and preparative applications as well as LC/MS
Very short column conditioning period
IC
Technical characteristics:
Ammonium - sulfonic acid modified silica; pore size 110; particle
sizes 1.8, 3 and 5m; carbon content 7%; pH stability 28.5
eluent
Recommended application:
Hydrophilic compounds such as organic polar acids and bases, polar
natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water soluble vitamins
RPC
NUCLEODUR HILIC
Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase
HPLC the most common analytical method is often
limited. Here, hydrophilic stationary phases provide an
additional tool for the separation of polar analytes in
HPLC.
The expression HILIC (Hydrophilic Interaction Chromatography) was firstly published by Andrew Alpert
in 1990 since then it took quite some efforts to develop robust and reproducible hydrophilic HPLC phases
for HILIC chromatography [A. Alpert, J. Chromatography 499
(1990), 177196].
HILIC combines the characteristics of the 3 major methods in liquid chromatography reversed phase (RPC),
normal phase (NPC) and ion chromatography (IC):
Stationary phases (adsorbents) are mostly polar
modifications of silica or polymers (SiOH, NH2, Diol,
(zwitter) ions, ) like in NPC
Retention characteristic
Commonly HILIC is described as partition chromatography or liquid-liquid extraction system between mobile and stationary phases. Versus a water-poor mobile
phase a water-rich layer on the surface of the polar stationary phase is formed. Thus, a distribution of the analytes between these two layers will occur. Furthermore
HILIC includes weak electrostatic mechanisms as well
as hydrogen donor interactions between neutral polar
molecules under high organic elution conditions. This
distinguishes HILIC from ion exchange chromatography
- main principle for HILIC separation is based on compounds polarity and degree of solvation.
Separation of uracil and naphthalene
Columns:
Eluent:
Flow rate:
Detection:
1
Peaks:
1. Uracil
2. Naphthalene
SiO2
SO3
CH3
136
min
MN Appl. No. 122911/122912
Overall NUCLEODUR HILIC provides excellent chromatographic features and is hereby the perfect choice
for separation of polar or charged compounds.
Column:
Eluent:
Flow rate:
Detection:
1 Inj.
2 Inj.
MN Appl. No. 123100
378 Inj.
793 Inj.
0
10
12
14
16
min
30mm
EC columns
2mm
3mm
4mm
4.6mm
EC guard columns*
ID
ID
ID
ID
760521.20
760521.30
760521.40
760521.46
NUCLEODUR HILIC, 3m
EC columns
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
NUCLEODUR HILIC, 5m
EC columns
2mm
3mm
4mm
4.6mm
EC guard columns*
CC guard columns**
ID
ID
ID
ID
50mm
75mm
100mm
125mm
150mm
250mm
760523.20 760525.20
760523.30
760523.40
760523.46
4 x 2mm: 761960.20
760526.20
760526.30
760526.40
760526.46
4 x 3mm: 761960.30
760528.20
particle size 3m
760532.20
760532.30
760532.40
760532.46
4 x 2mm: 761961.20
8 x 3mm: 761580.30
760534.20
760534.30
760534.40
760534.46
4 x 3mm:
8 x 4mm:
760531.20
760531.30
760531.40
760531.46
761961.30
761580.40
760533.20
760533.30
760533.40
760533.46
760530.20
760530.30
760530.40
760530.46
Ordering information
particle size 5m
760552.20
760552.30
760552.40
760552.46
4 x 2mm: 761962.20
8 x 3mm: 761590.30
760554.20
760554.30
760554.40
760554.46
4 x 3mm:
8 x 4mm:
760551.20
760551.30
760551.40
760551.46
761962.30
761590.40
760553.20
760553.30
760553.40
760553.46
760550.20
760550.30
760550.40
760550.46
EC columns in packs of 1, guard columns in packs of 3; for details see page 189
www.mn-net.com
EC
CC
2mm
3mm
4mm
4.6mm
4/2
8/3
4/3
8/3
4/3
8/4
4/3
8/4
137
Key features:
High retention capacity especially for very polar and unsaturated
compounds
Multi-mode column (RP and NP) widens scope of selectivity
Stable against hydrolysis at low pH (working range pH 18)
Columns:
Eluent:
Flow rate:
Detection:
Tricyclic antidepressants
steroids
organic acids
USP L10
Technical characteristics:
Cyanopropyl-modified high purity silica; pore size 110; particle sizes
3m and 5m; carbon content 7%; special endcapping, high reproducibility from lot to lot; different retention characteristics in comparison to C8 and C18
Recommended application:
Column:
125 x 4mm NUCLEODUR 100-5 CN-RP
Eluent:
acetonitrile water, 2% TFA pH 1 (50:50, v/v)
Flow rate: 1.0mL/min
Temperature: 25C
Detection: UV, 254nm
Injection:
5L
Peaks:
1. Benzamide
2. Dimethyl phthalate
3. Phenetole
4. o-Xylene
5. Biphenyl
2
3
2
4
6
1
0
1st injection
6
4
12 min
MN Appl. No. 119340
138
6 min
MN Appl. No. 119350
info@mn-net.com
10
20
30
6+7
2
40
min
10
15
20 min
Ordering information
Length
50mm
125mm
EC columns
EC guard columns*
CC guard columns**
2mm
3mm
4mm
4.6mm
ID
ID
ID
ID
EC columns
EC guard columns*
CC guard columns**
760157.20
760157.30
EC guard columns*
CC guard columns**
760156.40
760156.46
4 x 3mm: 761941.30
8 x 4mm: 761430.40
4 x 2mm: 761941.20
8 x 3mm: 761430.30
4mm ID
4.6mm ID
NUCLEODUR 100-5 CN
EC columns
250mm
760159.20
150mm
760152.40
760154.46
760152.46
4 x 3mm: 761944.30
8 x 4mm: 761420.40
4mm ID
4.6mm ID
EC columns in packs of 1, guard columns in packs of 3; for details see page 189
760149.40
760150.40
760149.46
760150.46
4 x 3mm: 761943.30
8 x 4mm: 761419.40
www.mn-net.com
EC
CC
2mm
3mm
4mm
4.6mm
4/2
8/3
4/3
8/3
4/3
8/4
4/3
8/4
139
Key features:
Multi-mode columns (for RP, NP and IC)
Stable against hydrolysis at low pH (working range pH 28), 100% stable in water; suitable for LC/MS
Widens scope of analytical HPLC into the polar range
Technical characteristics:
Aminopropyl modified high purity silica; pore size 110; particle sizes 3, 5 and 7m;
carbon content 2.5%; not endcapped
Recommended application:
Polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditions
USP L8
Normal phase chromatography (NP) with hexane, dichloromethane or 2-propanol as mobile phase for
polar compounds such as substituted anilines, esters, chlorinated pesticides
Reversed phase chromatography (RP) of polar compounds in aqueous-organic eluent systems
Ion exchange chromatography of anions and organic acids using conventional buffers and organic modifiers
Multi-mode columns
Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases
both feature the important advantage, that they can
be run in the RP mode using aqueous-organic eluent
mixtures as well as in the NP mode, e.g., with hexane
as mobile phase.
Reversed phase separation of sugars
Column:
250 x 4mm NUCLEODUR 100-5 NH2-RP
Eluent:
acetonitrile water (79:21, v/v)
Flow rate: 2mL/min
Detection: RI
Peaks:
1. Fructose
2. Glucose
3. Saccharose
4. Maltose
5. Lactose
1
Columns:
Eluent:
Flow rate:
Detection:
Peaks:
1. Cyclohexane
2. 1-Phenyldodecane
3. 1,2-Dimethylbenzene
4. Hexamethylbenzene
5. Naphthalene
6. Dibenzothiophene
7. 9-Methylanthracene
31
5
6
10
min
10 min
NUCLEODUR NH2, too, belongs to the so-called multimode columns. It can be used for RP chromatography
of polar compounds such as sugars in aqueous-organic
eluent systems, for NP chromatography of substituted aromatics or chlorinated pesticides with organic
140
Column:
250 x 4mm
NUCLEODUR
100-5 NH2-RP
Eluent:
acetonitrile
water (80:20, v/v)
Flow rate:
0.6mL/min
Temperature: 35C
Pressure:
30bar
Detection:
UV, 254nm
Peaks:
1. Thymine
2. Uracil
3. Cytosine
4. Adenine
Column:
Eluent:
Flow rate:
Detection:
Peaks:
1. Aminomethylphosphonic acid
(AMPA)
after 3872min
1st injection
0
min
2
3
min
LC/MS. Additionally, the high batch-to-batch reproducibility of NUCLEODUR NH2 enables reliable analyses especially for routine work.
Ordering information
Length
100mm
EC columns
EC guard columns*
CC guard columns**
2mm ID
4.6mm ID
EC columns
EC guard columns*
CC guard columns**
2mm
3mm
4mm
4.6mm
ID
ID
ID
ID
125mm
150mm
250mm
760740.20
760741.20
4 x 2mm: 761951.20
8 x 3mm: 761035.30
760742.46
760739.46
4 x 3mm: 761951.30
8 x 4mm: 761035.40
760732.20
760732.30
760732.40
760731.46
760732.46
4 x 3mm: 761953.30
8 x 4mm: 761137.40
4mm ID
4.6mm ID
760722.40
760722.46
760721.46
4 x 3mm: 761952.30
8 x 4mm: 761130.40
EC guard columns*
CC guard columns**
EC columns in packs of 1, guard columns in packs of 3; for details see page 189
www.mn-net.com
10
Based on superspherical NUCLEODUR this phase features a high pressure stability, which makes it the perfect choice for preparative separations as well as for
EC
CC
2mm
3mm
4mm
4.6mm
4/2
8/3
4/3
8/3
4/3
8/4
4/3
8/4
141
Key features:
Technical characteristics:
Unmodified high purity silica; pore size 110; particle sizes 3 to 50m; pore volume 0.9mL/g;
surface area (BET) 340m2/g; pH stability 28; metal content < 10ppm (see page 110)
Recommended application:
Polar and midpolar compounds under normal phase conditions
USP L3
Ordering information
Eluent in column n-heptane
Length
50mm
125mm
150mm
NUCLEODUR 100-3
250mm
particle size 3m
EC columns
760170.46
4.6mm ID
760172.46
4 x 3mm: 761966.30
8 x 4mm: 761007.40
EC guard columns*
CC guard columns**
NUCLEODUR 100-5
EC columns
EC guard columns*
CC guard columns**
particle size 5m
4mm ID
4.6mm ID
VarioPrep columns
VP guard columns*
760173.46
10mm ID
21mm ID
40mm ID
760023.46
760007.40
760007.46
760012.46
4 x 3mm: 761967.30
8 x 4mm: 761055.40
762077.100
762077.210
762078.100
762078.210
10 x 8mm: 762094.80
15 x 32mm: 762330.320
762007.100
762007.210
762075.400
762007.400
10 x 16mm: 762094.160
EC
CC
VP
2mm
3mm
4mm
4.6mm
4/2 (3)
8/3 (3)
4/3 (3)
8/3 (3)
4/3 (3)
8/4 (3)
4/3 (3)
8/4 (3)
8, 10mm
16, 21mm
32, 40mm
50mm
10/8 (2)
718251
10/16 (2)
718256
15/32 (1)
718253
15/50 (1)
718255
142
info@mn-net.com
Designation
Test mixture for reversed phase
columns in acetonitrile *
Pack of
1mL
REF
722394
www.mn-net.com
143
1.7m
solid core of
silicon dioxide
0.5m
W!
E
N
Pore size 90; particle size 2.7m (core 1.7m); specific surface 130m2/g
lower back pressure enables use on conventional LC
systems
Pressure stability 600bar
Demands on HPLC separations are constantly increasing with respect to separation efficiency, detection limits, and the time requirements for each
analysis.
ki
k i + 1
N 1
Rs = resolution
= selectivity
ki = retention
N = theoretical plates N 1/dP
dP = particle size
Columns:
50 x 4mm
NUCLEOSHELL RP 18, 2.7m
NUCLEODUR C18 Gravity, 3m
NUCLEODUR C18 Gravity, 1.8m
Eluent:
acetonitrile water (60:40, v/v)
Flow rate: 1mL/min
Temperature: 25C
Detection: UV, 254nm
1
Peaks:
RS = 2.47
104 bar
1. Naphthalene
2. Ethylbenzene
Better resolution at
lower back pressure
and shorter
retention time
0.5
RS = 1.71
88 bar
22
0.0
RS = 2.17
190 bar
1.0
1.5
2.0
2.5
min
144
dp
[m]
2.7
1.8
3
5
L
[m]
1
1
1
1
HETP
[m]
4
4.5
7.5
12.5
Efficiency
[plates/m]
250000
222222
133333
80000
L
[mm]
100
100
150
250
Rs
25000
22000
20000
20000
112%
105%
100%
100%
Analysis
time
40%
40%
60%
100%
info@mn-net.com
20
18
16
14
Van-Deemter plots
B
h = A + --- + C u
u
NUCLEODUR C18 Gravity, 3 m
Poroshell 120 EC-C18
12
NUCLEOSHELL RP 18
10
Kinetex C18
6
4
Columns:
Eluent:
Temperature:
Sample:
2
0
Pressure drop
10
600
p =
500
Pressure [bar]
50 x 4.6 mm
CH3CN H2O (70:30, v/v)
25 C
Acenaphthene
12
LC u
dp2
p = pressure drop
= flow resistance (nondimensional)
LC = column length
= viscosity
u = linear velocity
dp = particle diameter
400
300
Kinetex C18
NUCLEOSHELL RP18
200
Columns:
50 x 4.6 mm
Eluent:
CH3CN H2O (70:30, v/v)
Temperature: 25 C
100
0
With conventional fully porous particles the mass transfer between stationary and mobile phase usually results
in peak broadening at higher flow rates (C-term in van
Deemter equation). The short diffusion paths in the
10
12
Core-shell particle technology from MACHEREY-NAGEL is an alternate route to gain highest column
efficiency and resolution in HPLC at short run time, but with moderate back pressure.
www.mn-net.com
145
Columns:
100
pH 10
12000
80
pH 1
60
10000
Plates (toluidine)
Column:
NUCLEOSHELL
Kinetex
40
20
0
5000
10000
15000
20000
Column volumes
25000
8000
6000
2000
30000
Stability test:
Column:
Eluent:
Temperature stability
Flow rate:
Detection:
Peaks:
1. Phenol
2. Naphthalene
NUCLEOSHELL
Ascentis Express
Poroshell 120
Kinetex
4000
5000
10000
15000
Column volumes
20000
Batch-to-batch reproducibility
Column:
Eluent:
2 3
38 h
34 h
Charge 1
30 h
26 h
22 h
0
Effficiency test:
Eluent:
acetonitrile water (60:40, v/v)
Flow rate:
0.33mL/min; temperature 25C
Detection:
UV, 254nm
Analyte: anthracene
HETP [m]
Start (t = 0)
5.2
End (t = 40h)
5.2
Charge 3
Asymmetry
0.98
1.01
146
Charge 2
min
10
min
info@mn-net.com
The peak capacity is a measure for the number of sample analytes that can be separated on HPLC columns per
time unit. Narrow peaks increase the peak capacity and
thus the efficiency of the analytical column.
Peak capacity
Columns:
100 x 4.6mm
NUCLEOSHELL RP 18, 2.7m
NUCLEODUR C18 Gravity, 1.8m
NUCLEODUR C18 Gravity, 3m
NUCLEODUR C18 Gravity, 5m
Eluent:
A) acetonitrile, B) water, 40100% A in 4min
Flow rate:
1.5mL/min
Temperature: 25C
Detection:
UV, 230nm
Peaks:
1. Acetophenone
4. Butyrophenone
2. Benzoin
5. Benzophenone
3. Propiophenone
6. Valerophenone
3
2
4
5
Loading capacity
Column:
Eluent:
Flow rate:
Detection:
Peaks:
1. Valerophenone
9 g
800
600
400
R2 = 0.9991
200
0
3 g
0
10
0.9 g
0.3 g
0
min
min
Max. pressure
[bar]
255
450
214
142
NUCLEOSHELL, 2.7m
NUCLEODUR, 1.8m
NUCLEODUR, 3m
NUCLEODUR, 5m
nc (normalized)
250
216
200
150
100
100
125
163
50
0
5 m
3 m 1.8 m 2.7 m
NUCLEODUR NUCLEOSHELL
nc = 1 + (
Resolution
(4, 5)
5.45
4.14
2.97
2.30
tg
0.4
Retention time
0.2
Efficiency
(at 10 % peak height)
10
NUCLEOSHELL modifications
Peak width
(at 50 % peak height)
The example shows, that in comparison with totally porous NUCLEODUR silica (1.8m) NUCLEOSHELL provides 33% higher peak capacity.
Loading capacity
0.6
Area
Peak capacity
The program of NUCLEOSHELL surface modifications now comprises the following phases:
NUCLEOSHELL RP 18
NUCLEOSHELL PFP
NUCLEOSHELL HILIC
147
Key features:
Technical characteristics:
Octadecyl modification, multi-endcapped; pore size 90,
particle size 2.7m, carbon content 7.5%
Recommended application:
Overall sophisticated analytical
separations, e.g., analgesics, antiinflammatory drugs, antidepressants;
herbicides; phytopharmaceuticals;
immunosuppressants
USP L1
ration of basic and other ionizable analytes. The extremely reduced silanol activity of the phase can be
demonstrated by applying basic analytes, such as tricyclic antidepressants. The chromatogram below shows
a sharp elution profile (superior resolution!) of these
highly polar compounds with an excellent asymmetry
value for amitriptyline of 1.12.
Columns:
50 x 4.6mm
NUCLEOSHELL RP 18, 2.7m
Ascentis Express C18
Kinetex 2.6m C18
Poroshell 120 EC-C18
Eluent:
methanol acetonitrile 25mmol/L KH2PO4, pH 7
(22.5:22.5:55, v/v/v)
Flow rate:
2mL/min
Pressure:
224bar, 239bar, 248bar, 212bar
Temperature: 40C
Detection:
UV, 220nm
NUCLEOSHELL
Ascentis Express
Kinetex
Poroshell
Asymmetry
(amitriptyline)
1.12
2.07
1.33
1.05
Peaks:
1. Protriptyline
2. Desipramine
3. Maprotiline
4. Nortriptyline
5. Doxepin
6. Imipramine
7. Amitriptyline
8. Clomipramine
9. Trimipramine
Resolution
(8, 9)
3.35
1.91
n.a.
1.95
NUCLEOSHELL
2
Ascentis
Kinetex
5
3
Poroshell
0
min
7
8
148
10
12
14
16
18
min
info@mn-net.com
10
23
6 7 8
10
0.0
0.4
12
min
11 13
12
2.5 min
270 bar
23
10
6 7 8
0.8
1.6
1.2
2.0
min
Ordering information
12
11 13
50mm
100mm
150mm
EC guard columns*
2mm ID
3mm ID
4mm ID
4.6mm ID
763134.20
763134.30
763134.40
763134.46
763138.20
EC columns in packs of 1, guard columns in packs of 3; for details see page 189
763136.20
763136.30
763136.40
763136.46
4 x 3mm:
www.mn-net.com
EC
2mm
3mm
4mm
4.6mm
4/2
4/3
4/3
4/3
763138.30
149
Key features:
Technical characteristics:
Phase with pentafluorophenyl-propyl modification, multi-endcapping;
pore size 90, particle size 2.7m; carbon content ~3%;
pH stability 19; suitable for LC/MS
Recommended application:
Orthogonality in selectivity
Column:
Eluent:
Flow rate:
Detection:
Sample:
Ethylbenzene
NUCLEOSHELL PFP
120
100
While a typical C18 phase just provides hydrophobic interactions between stationary phase and analyte NUCLEOSHELL PFP offers four different retention
mechanisms: polar interactions (H bonds), dipole-dipole interactions, - interactions and hydrophobic
interactions. Especially the pronounced ion exchange
capacity and distinct steric selectivity are typical for the
character of fluorinated phases.
80
60
% initial retention
% initial plates
40
20
2000
Column volumes
4000
6000
Columns:
100 x 4.6mm
NUCLEOSHELL RP 18, 2.7m
NUCLEOSHELL PFP, 2.7m
Eluent:
A) acetonitrile + 0.1% formic acid
B) 0.1% formic acid
1035% A in 2.5min, 35-50% A in 2min
Flow rate:
1.7mL/min
Temperature: 25C
Detection:
UV, 280nm
Peaks:
1. Atenolol
2. Pindolol
3. Metroprolol
4. Labetalol
5. Alprenolol
6. Propranolol
150
3
3
4
info@mn-net.com
min
3
1
3
NUCLEOSHELL PFP
3 mL/min
10
min
10
20
30
40
min
NUCLEOSHELLPFP combines the benefits of core-shell technology, high stability, and orthogonal selectivity. Thus it is a useful complementary tool for highly efficient separations especially of isomers,
halogenated, aromatic and/or polar compounds.
Ordering information
50mm
100mm
150mm
EC guard columns*
2mm ID
3mm ID
4mm ID
4.6mm ID
763534.20
763534.30
763534.40
763534.46
763538.20
EC columns in packs of 1, guard columns in packs of 3; for details see page 189
763536.20
763536.30
763536.40
763536.46
4 x 3mm:
www.mn-net.com
EC
2mm
3mm
4mm
4.6mm
4/2
4/3
4/3
4/3
763538.30
151
HILIC
te
aly
an
NPC
ad
so
rbe
nt
IC
Technical characteristics:
Ammonium - sulfonic acid modified silica; pore size 90,
particle size 2.7m; carbon content 1.3%;
pH stability 28.5; suitable for LC/MS
eluent
RPC
Recommended application:
Hydrophilic compouds such as polar organic acids and bases, polar
natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water-soluble vitamins
Good separation of polar compounds like the physiologically important substances creatine and creatinine
can be achieved on NUCLEOSHELL HILIC as well as on
NUCLEODUR HILIC, 1.8m at similar retention, but
much lower back pressure.
Separation of creatine and creatinine
SiO2
NUCLEOSHELL HILIC
CH3
+N
SO3
CH3
0.0
0.2
0.4
0.6
0.8
min
152
info@mn-net.com
1.0
Separation of catecholamines
1 min
4 mL
4.5 min
4.5 mL
12 min
12 mL (solvent
consumption
1
per run)
4
3
4
3
0b n
ar
5
6
7
Core-Shell
Fully porous
0
10
min
NUCLEOSHELL HILIC provides stable and reproducible chromatography, comprising all the benefits of a
state-of-the-art core-shell silica.
Ordering information
50mm
100mm
150mm
EC guard columns*
2mm ID
3mm ID
4mm ID
4.6mm ID
763334.20
763334.30
763334.40
763334.46
763338.20
763336.20
763336.30
763336.40
763336.46
4 x 3mm:
EC
www.mn-net.com
2mm
3mm
4mm
4.6mm
4/2
4/3
4/3
4/3
763338.30
153
Still found in many analytical and preparative applications, it is an absolutely reliable choice in HPLC
Largest variety of modified HPLC silicas available
Physical properties
NUCLEOSIL is manufactured with different pore diameters (50, 100, 120, 300, 500, 1000 and 4000) and
particle sizes from 3m (only NUCLEOSIL 50, 100 and
120) to 10m with very narrow fractionation.
All narrow-pore NUCLEOSIL packings are stable up to
500bar (7250psi), the wide-pore NUCLEOSIL silicas
are stable up to 300 or 400bar (4200 or 5600psi).
154
NUCLEOSIL modifications
info@mn-net.com
Stability
Structure
NUCLEOSIL
(Si-O2)n
pH 29
15% C USPL1
Octadecyl phase,
high density
monomeric
modification,
endcapping
C18 HD
20% C USPL1
C18 AB
pH 19
25% C USPL1
C18 Nautilus
Octadecyl phase,
embedded polar
group, endcapping
16% C USPL60
Protect I
Special RP phase,
protective polar
group, monomeric
modification,
endcapping
pH 28
up to
100%
H 2O
pH 28
11% C
C8 ec
Octyl phase,
medium density
modification,
endcapping
pH 28
9% C USPL7
C8
C8 HD
Octyl phase,
no endcapping
8.5% C USPL7
Octyl phase, high
density modification, endcapping
pH 28
pH 28
13% C USPL7
C4
Butyl phase,
medium density
modification,
endcapping
pH 28
~ 2% C USPL26
C2
Dimethyl phase
3.5% C USPL16
www.mn-net.com
pH 28
Si OH
Si O Si(CH )
3 3
Page
hydrophobic
(van der Waals)
interactions
157
158
steric interactions
and hydrophobic
interactions
158
hydrophobic
interactions and polar
interactions
158
hydrophobic
interactions and polar
interactions
160
Pol
Si OH
Pol
Si O Si(CH )
3 3
pro
Si OH
pro
Si O Si(CH3)3
Si OH
Si O Si(CH )
3 3
Si OH
hydrophobic
(van der Waals)
interactions
160
hydrophobic
(van der Waals)
interactions
160
noticeable residual
silanol interactions
Si OH
hydrophobic
(van der Waals)
interactions
Si OH
Si O Si(CH )
3 3
Si OH
Si
Si
O
O
Si OH
Si(CH3)2
161
hydrophobic
(van der Waals)
interactions
161
hydrophobic
(van der Waals)
interactions
162
residual silanol
interactions
noticeable residual
silanol interactions
Octadecyl phase,
special crosslinked
modification,
endcapping
NUCLEOSIL NUCLEOSIL
(Si-O2)n
(Si-O2)n
pH 28
NUCLEOSIL
(Si-O2)n
C18
Octadecyl phase,
medium density
modification,
endcapping
NUCLEOSIL
(Si-O2)n
NUCLEOSIL RP-Phasen
Separation principle
155
C6H5 ec
Phenyl phase,
medium density
modification,
endcapping
Stability
pH 28
8% C USPL11
C6H5
Phenyl phase,
no endcapping
8% C USPL11
pH 28
Structure
NUCLEOSIL NUCLEOSIL
(Si-O2)n
(Si-O2)n
Si OH
Si O Si(CH )
3 3
Si OH
Si OH
CN/CN-RP
NO2
OH (Diol)
NH2/NH2-RP
N(CH3)2
SA
Cyano (nitrile)
phase
USPL10
Nitrophenyl
Diol
USPL20
Amino
USPL8
Dimethylamino
Sulfonic acid,
strongly acid cation exchanger
pH 28
pH 28
pH 28
pH 28
pH 28
pH 28
(SCX) USPL9
SB
pH 28
(SAX) USPL14
SiOH
Unmodified
spherical silica
USPL3
pH 28
C N
NO2
Si OH
Si OH
O
Si OH
O
Si OH
NO2
OH
OH
OH
OH
NH2
Si OH
NH2
Si OH
Si OH CH3
N
CH3
Si OH
SO3Na
Si OH
Si OH
SO3Na
Si OH
Si OH
Si OH
Si OH
CH3 Cl
+
N CH3
CH3
Separation principle
Page
interactions
and hydrophobic
interactions
200*
interactions
and hydrophobic
interactions
162
noticeable residual
silanol interactions
interactions,
polar interactions
and hydrophobic
interactions
164
interactions,
polar interactions
and hydrophobic
interactions
200*
polar interactions
(hydrogen bonds)
162
163
polar and
hydrophobic
interactions, weak
ion exchange
interactions
163
164
165
polar interactions
165
156
info@mn-net.com
-(CH2)17CH3
NUCLEOSIL C18 AB
Crosslinked hydrophobic phase, polymeric modification, inert towards acidic and basic substances with
high affinity for silica; pH stability 19; carbon content 25%; distinct steric selectivity USP L1
Corresponding NUCLEODUR phases see C18 Isis page 120
Ordering information
100mm
125mm
150mm
720098.46
4.6mm ID
EC columns
2mm
3mm
4mm
4.6mm
VarioPrep columns
ID
ID
ID
ID
720141.40
720141.46
720949.46
720002.20
720002.30
720002.40
720002.46
VarioPrep columns
8mm
10mm
16mm
21mm
ID
ID
ID
ID
www.mn-net.com
720133.40
720133.46
720120.20
720120.30
720120.40
720120.46
720014.20
720014.30
720014.40
720014.46
715340.100
715340.210
CC guard col.**
721473.30
721829.40
EC guard col.*
721022.30
721022.30
CC guard
columns**
721866.40
721866.40
10mm ID
21mm ID
EC columns
EC guard col.*
EC columns
720841.46
Guard columns
EC columns
4mm ID
4.6mm ID
250mm
EC guard col.*
721074.20
721074.30
721074.30
721074.30
CC guard col.**
721602.30
721602.30
721602.40
721602.40
VP guard col.***
715360.80
715360.160
720110.46
720018.40
720018.46
715332.80
715332.100
715332.160
715332.210
VP guard col.***
715360.80
715360.80
715360.160
715360.160
157
100mm
125mm
EC columns
4mm ID
4.6mm ID
4mm ID
4.6mm ID
720701.46
720149.40
720149.46
4mm ID
4.6mm ID
720040.40
720040.46
720140.46
720051.40
720051.46
720740.46
720730.46
720191.40
720191.46
ID
ID
ID
ID
158
4mm ID
4.6mm ID
CC guard col.**
721783.40
721783.40
CC guard col.**
721494.40
721494.40
720294.46
720280.40
720280.46
EC guard col.*
721072.30
721072.30
CC guard col.**
721853.40
721853.40
720305.46
EC columns
EC guard col.*
721196.30
721196.30
720193.46
EC guard col.*
721070.30
721070.30
EC columns
720041.40
720041.46
720043.40
720043.46
4mm ID
4.6mm ID
4mm ID
4.6mm ID
CC guard col.**
721606.40
721606.40
EC columns
EC guard col.*
721075.30
721075.30
720042.40
4mm ID
4mm ID
4.6mm ID
720055.40
720055.46
EC columns
EC columns
720023.40
720023.46
EC columns
Guard columns
EC columns
250mm
EC columns
150mm
720472.40
720472.46
720936.20
720936.30
720936.40
720936.46
EC guard col.*
721073.20
721073.30
721073.30
721073.30
CC guard col.**
721603.30
721603.30
721603.40
721603.40
720471.46
EC guard col.*
721649.30
721649.30
CC guard col.**
721611.40
721611.40
info@mn-net.com
100mm
125mm
150mm
250mm
EC columns
4mm ID
4.6mm ID
720430.40
720430.46
720432.46
Many biologically interesting molecules can not be separated using conventional narrow pore silicas with pore
sizes of about 100.
EC guard col.*
721133.30
721133.30
720431.40
720431.46
250mm
CC guard col.**
721140.40
721140.40
Length
EC columns
Guard columns
Guard columns
CC guard col.**
721608.40
721608.40
VarioPrep columns
VP guard col.***
715806.100
715806.210
10mm ID
21mm ID
715360.80
715360.160
EC columns
720074.46
4.6mm ID
EC columns
720077.46
4.6mm ID
EC columns
EC guard col.*
721085.30
721085.30
720065.40
720065.46
4mm ID
4.6mm ID
720085.46
4.6mm ID
EC and VarioPrep columns in packs of 1, guard columns see below
EC
CC
VP
2mm
3mm
4mm
4.6mm
4/2 (3)
8/3 (3)
4/3 (3)
8/3 (3)
4/3 (3)
8/4 (3)
4/3 (3)
8/4 (3)
8, 10mm
16, 21mm
32, 40mm
50mm
10/8 (2)
718251
10/16 (2)
718256
15/32 (1)
718253
15/50 (1)
718255
www.mn-net.com
159
Ordering information
Length:
125mm
150mm
250mm
4mm ID
4.6mm ID
EC
CC
guard columns* guard columns**
720174.46
720170.40
720170.46
721157.30
721157.30
721154.40
721154.40
-(CH2)7-CH3
Ordering information
Length:
125mm
NUCLEOSIL 100-5 C8 ec
4mm ID
4.6mm ID
NUCLEOSIL 100-7 C8
4.6mm ID
NUCLEOSIL 100-10 C8
4mm ID
4.6mm ID
160
250mm
EC guard
columns*
CC guard
columns**
4.6mm ID
NUCLEOSIL 100-5 C8
150mm
721096.30
721805.40
720001.40
720001.46
720990.46
720013.40
720013.46
721194.30
721194.30
721601.40
721601.40
info@mn-net.com
EC columns
Length:
NUCLEOSIL 120-3 C8
125mm
150mm
EC guard
columns*
250mm
CC guard
columns**
720071.40
720071.46
4mm ID
4.6mm ID
NUCLEOSIL 120-5 C8
721093.30
721093.30
720214.46
721785.40
721785.40
720050.40
720050.46
4mm ID
4.6mm ID
NUCLEOSIL 300-5 C8
720735.46
720052.40
720052.46
721095.30
721095.30
721787.40
721787.40
4.6mm ID
NUCLEOSIL 100-5 C8 HD
721061.30
721101.40
720196.40
720196.46
721071.30
721071.30
721500.40
721500.40
-(CH2)3CH3
For butyl phases for biochemical separations please refer to page 182.
Ordering information
Length:
EC guard
columns*
250mm
NUCLEOSIL 120-5 C4
CC guard
columns**
4mm ID
4.6mm ID
720096.46
4.6mm ID
NUCLEOSIL 300-5 C4
721083.30
721889.40
720059.40
720059.46
4mm ID
4.6mm ID
721916.30
721916.30
721607.40
721607.40
EC
CC
2mm
3mm
4mm
4.6mm
4/2
8/3
4/3
8/3
4/3
8/4
4/3
8/4
www.mn-net.com
161
-(CH3)2
Retention times are much shorter than for the other RP phases
Ordering information
Length:
EC guard
columns*
250mm
NUCLEOSIL 100-7 C2
CC guard
columns**
720089.46
4.6mm ID
721030.30
721069.40
-(CH2)3
Relatively nonpolar, non-endcapped phases for RP and ion pairing chromatography USP L11
Polarity similar to C8, but with different selectivity for PAHs, polar aromatics, fatty acids etc.
pH stability at 20C: 28; carbon content 8%
Ordering information
Length:
EC guard
columns*
250mm
CC guard
columns**
4.6mm ID
4mm ID
4.6mm ID
720956.46
721137.30
721862.40
720019.40
720019.46
(CH2)3
CH2
CH
CH2
OH OH
Less polar than unmodified silica, very easily wettable with water
pH stability at 20C: 2-8; carbon content 5%
Ordering information
Eluent in column is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is
necessary to rinse the columns with THF first.
EC columns
Length:
250mm
162
720143.46
EC guard
columns*
CC guard
columns**
721478.40
info@mn-net.com
-(CH2)3NH2
Aminopropyl modified polar silica phase pH stability at 20C: 28; carbon content 3.5% USP L8
Corresponding NUCLEODUR phases see page 140
NP chromatography with hexane, dichloromethane or 2-propanol as mobile phase for polar compounds
such as substituted anilines, esters, chlorinated pesticides
RP chromatography of polar compounds like carbohydrates in aqueous-organic eluent systems
Anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or
phosphate) in conjunction with organic modifiers (e.g., acetonitrile)
Ordering information
Eluent in column is n-heptane (except for NH2 RP). When using an eluent which is not miscible with n-heptane
(e.g., water), it is necessary to rinse the column with THF first.
Length:
EC guard
columns*
250mm
CC guard
columns**
4.6mm ID
4.6mm ID
720275.46
721933.30
720095.46
721020.30
EC column
721122.40
721605.40
4.6mm ID
721155.30
721605.40RP
-(CH2)3N(CH3)2
Weakly basic anion exchanger for the separation of many anions; pH stability at 20C: 28; 4% C
Can also be used in a similar way as the NH2 phase
Ordering information
Eluent in column is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is
necessary to rinse the columns with THF first.
EC columns
Length:
720994.46
4.6mm ID
EC guard
columns*
250mm
CC guard
columns**
721610.40
EC
CC
2mm
3mm
4mm
4.6mm
4/2
8/3
4/3
8/3
4/3
8/4
4/3
8/4
www.mn-net.com
163
-(CH2)3 CN
Ordering information
Eluent in column (except for NUCLEOSIL 100-5 CN-RP) is n-heptane. When using an eluent which is not
miscible with n-heptane (e.g., water), it is necessary to rinse the column with THF first.
EC columns
Length:
NUCLEOSIL 100-5 CN
250mm
EC guard
columns*
CC guard
columns**
4mm ID
4.6mm ID
721078.30
721078.30
721604.40
721604.40
4mm ID
4.6mm ID
NUCLEOSIL 100-10 CN
721039.30
721039.30
721917.40
721917.40
4mm ID
4.6mm ID
NUCLEOSIL 120-7 CN
4mm ID
4.6mm ID
NUCLEOSIL SA phases
-(CH2)3
SO3Na
Strongly acidic cation exchanger (SCX) with benzenesulfonic acid modification USPL9
Capacity ~ 1meq/g; pH stability at 20C: 28; carbon content 6.5%
Ordering information
EC columns
125mm
Length:
150mm
250mm
NUCLEOSIL 100-5 SA
4mm ID
4.6mm ID
164
720709.46
720182.46
720097.40
720097.46
EC guard
columns*
CC guard
columns**
721487.40
721487.40
info@mn-net.com
EC columns
Length:
150mm
125mm
EC guard
columns*
250mm
NUCLEOSIL 100-10 SA
CC guard
columns**
720028.46
4.6mm ID
NUCLEOSIL SB phases
-(CH2)3
CH2N+(CH3)3Cl
Strongly basic anion exchanger (SAX) with quaternary ammonium modification USPL14
Capacity ~ 1meq/g; pH stability at 20C: 28; carbon content 10%
Ordering information
125mm
EC guard
columns*
250mm
NUCLEOSIL 100-5 SB
4.6mm ID
720989.46
720183.46
720996.46
NUCLEOSIL 100-10 SB
721885.40
720029.46
4.6mm ID
NUCLEOSIL SiOH
CC guard
columns**
unmodified silica
For physical properties of unmodified NUCLEOSIL materials please see page 199;
maximum working pressure for the EC columns listed below is 400bar.
Ordering information
Eluent in column is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is
necessary to rinse the column with THF first.
EC columns
Length:
NUCLEOSIL 50-5
720093.46
4.6mm ID
NUCLEOSIL 100-5
720099.46
4.6mm ID
EC guard
columns*
250mm
CC guard
columns**
EC columns
721600.40
721872.40
EC
CC
2mm
3mm
4mm
4.6mm
4/2
8/3
4/3
8/3
4/3
8/4
4/3
8/4
www.mn-net.com
165
Phase
100 RP 8, 5m
100 RP 8 ec, 5m
100 RP 18, 5m
100 RP 18 ec, 5m
60 RP select B, 5m
Particle size
nom.
nom.
nom.
nom.
nom.
5m
5m
5m
5m
5m
Pore size
100
100
100
100
60
Modification
octyl
octyl
octadecyl
octadecyl
octyl
Endcapped
Carbon content
12.5%
12.5%
21%
21%
12%
ChromCart columns require the CC connecting kit (REF 721690, see page 192).
Ordering information
LiChrospher 100 RP 8, 5m
125mm
728025.20
728025.30
728025.40
728025.46
2mm
3mm
4mm
4.6mm
ID
ID
ID
ID
2mm
3mm
4mm
4.6mm
ID
ID
ID
ID
728028.20
728028.30
728028.40
728028.46
2mm
3mm
4mm
4.6mm
ID
ID
ID
ID
728031.20
728031.30
728031.40
728031.46
2mm
3mm
4mm
4.6mm
ID
ID
ID
ID
728034.20
728034.30
728034.40
728034.46
2mm
3mm
4mm
4.6mm
ID
ID
ID
ID
LiChrospher 60 RP select B, 5m
728037.20
728037.30
728037.40
728037.46
150mm
250mm
Guard columns
728027.46
728026.20
728026.30
728026.40
728026.46
728051.30
728051.30
728051.40
728051.40
728030.46
728029.20
728029.30
728029.40
728029.46
728052.30
728052.30
728052.40
728052.40
728033.46
728032.20
728032.30
728032.40
728032.46
728053.30
728053.30
728053.40
728053.40
728036.46
728035.20
728035.30
728035.40
728035.46
728054.30
728054.30
728054.40
728054.40
728039.46
728038.20
728038.30
728038.40
728038.46
728055.30
728055.30
728055.40
728055.40
8mm ChromCart guard column cartridges in packs of 3, all other columns in packs of 1.
166
info@mn-net.com
Environmental analysis
Anion exchange chromatography
of inorganic anions
RP chromatography of PAHs
Recommended column
NUCLEOGEL Anion I
NUCLEOSIL Anion II
NUCLEODUR C18 PAH
NUCLEOSIL 100-5 C18 PAH
Enantiomer separation
NUCLEODEX -PM, -PM, -PM
and -OH
NUCLEOCEL DELTA
Ligand exchange
NUCLEOSIL CHIRAL-1
Charge-transfer-, dipole-dipole
interactions and others
Enantioselective binding to chiral
protein surface structures
NUCLEOSIL CHIRAL-2,
NUCLEOSIL CHIRAL-3
Page
171
168
170
172
174
176
177
RESOLVOSIL BSA-7
175
NUCLEOGEN DEAE
178
180
NUCLEOGEL SAX
181
NUCLEOGEL SCX
181
Biological macromolecules
NUCLEOSIL MPN
Water-insoluble compounds
www.mn-net.com
NUCLEOGEL GPC
182
183
184
184
185
186
187
187
188
167
Base material NUCLEODUR silica, particle sizes 1.8 and 3m, pore size 110; polymeric coating USP L1
Allows efficient gradient separation of the 16 PAHs according to EPA
Detection of separated PAHs with UV (250280nm), diode array or fluorescence detection at different
wavelengths for excitation and emission (acenaphthylene cannot be analyzed with fluorescence detection)
Analysis of 16 EPA PAHs with or without acetonitrile
10
6
9
5
16
1
11
275
350
375
425
10
14 15
335
440
12
6 7
13
ex
em
3 min
275
350
375
425
335
440
315
405
330
420
375
460
16
14
15
ex
em
11 12
13
Peaks:
1. Naphthalene
2. Acenaphthylene
(not detectable by
fluorescence)
3. Acenaphthene
4. Fluorene
5. Phenantrene
6. Anthracene
7. Fluoranthene
8. Pyrene
9. Benz[a]anthracene
10. Chrysene
11. Benzo[b]fluoranthene
12. Benzo[k]fluoranthene
13. Benzo[a]pyrene
14. Dibenz[ah]anthracene
15. Benzo[ghi]perylene
16. Indeno[1,2,3-cd]pyrene
345
420
300 nm
500 nm
10
min
100mm
125mm
150mm
EC guard
columns*
250mm
760773.20
760773.30
760773.40
3mm ID
4mm ID
760783.30
760783.40
168
CC guard
columns**
761970.20
761970.30
761970.30
760784.30
760784.40
EC
CC
760785.30
760785.40
2mm
4/2
8/3
760786.30
760786.40
3mm
4/3
8/3
761971.30
761971.30
4mm
4/3
8/4
4.6mm
4/3
8/4
761780.30
761780.40
718966
721359
info@mn-net.com
0 No. 123840
1
MN Appl.
5
7
2-me-n
1-me-n
10 12
11
9
16
14 15
13
ex
em
2
275
350
345
420
6
300
500
nm
nm
7 min
benz[a]anthracene
benzo[a]pyrene
125 x 4mm
NUCLEODUR C18 PAH,
3m
Eluent:
A) methanol water
(70:30, v/v)
B) acetonitrile
020% B in 1.5min,
2050% B in 1.5min,
50100% B in 1.0min,
100%B for 3min,
1000% B in 0.5min
Flow rate:
1.5mL/min
Temperature: 35C
Injection:
UV: 1L,
fluorescence: 0.5L
Detection:
UV, 254nm
fluorescence
(see chromatogram)
Peaks:
(concentrations 10ng/L per compound)
1.16. see opposite page
1-me-n: 1-methylnaphthalene
2-me-n: 2-methylnaphthalene
References
www.mn-net.com
169
Base material NUCLEOSIL silica, particle size 5m, pore size 100; polymeric coating USP L1
Recommended for efficient gradient separation of the 16 PAHs according to EPA
Detection of the separated PAH with UV (250280nm), diode array or fluorescence detection at different
wavelengths for excitation and emission (acenaphthylene cannot be analyzed with fluorescence detection)
Separation of the PAH standard according to EPA (REF 722393)
7
4
10
11
14
12
13
15
150mm
250mm
EC guard
columns*
CC guard
columns**
720923.30
720923.40
720117.20
720117.30
720117.40
720117.46
721168.20
721168.30
721168.30
721168.30
721599.30
721599.30
721599.40
721599.40
10
16
15
min
Ordering information
Length
ID
ID
ID
ID
EC
CC
2mm
3mm
4mm
4.6mm
4/2
8/3
4/3
8/3
4/3
8/4
4/3
8/4
722393
170
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NUCLEOGEL Anion I
Strongly basic polymer-based anion exchanger, particle size 10m; pH stability 114
Eluent in column 4mmol/L salicylate buffer pH 7.8
NUCLEOSIL Anion II
Base material NUCLEOSIL silica, particle size 10m, pore size 300
strongly basic anion exchanger, exchange capacity 50eq/g, pH stability 27.5
Column:
Eluent:
Flow rate:
Detection:
Peaks:
1. F
2. Cl
3. NO2
4. Br
5. NO3
6. PO43
7. SO42
1
23
10 min
10 min
Ordering information
Length
120mm
4.6mm ID
719533
250mm
Column:
Eluent:
Flow rate:
Detection:
Peaks:
1. H2PO4
2. Cl
3. NO2
4. NO3
5. SO42
Guard columns
NUCLEOGEL Anion I
Valco type columns
719543
NUCLEOSIL Anion II
EC columns
4mm ID
720094.40
721452.40
NUCLEOGEL Anion I Valco type guard columns measure 21 x 4mm and require the guard column holder C, REF 719538, see
page 193 (columns in packs of 1, guard columns in packs of 2).
As guard columns for NUCLEOSIL Anion II EC columns use 8 x 4mm ChromCart cartridges with guard column adapter EC,
REF 721359 (see page 191, columns and guard column cartridges in packs of 1).
www.mn-net.com
171
Base material NUCLEOSIL silica, particle size 5m, pore size 100
modified cyclodextrins as chiral selectors
Separation based on hydrogen bonds and dipole interactions between functional groups of the analyte and
hydroxyl groups of the cyclodextrin
Examples for successful enantiomer separations: chlorthalidone and other compounds,
which require free hydroxyl groups for enantioselective interactions
Eluent in column CH3OH 0.1% TEAA pH 4 (55:45)
Spacer
OR
OR
O
RO
OR O
RO
O
OR
OR
O RO
OR
X
O
OR
OR O
RO
RO
RO
O
OR
NUCLEODEX phases are especially suited for the control of optical purity, but also for semipreparative separations and for the analysis of positional and cis-trans isomers.
For numerous separations on NUCLEODEX phases please visit our website: www.mn-net.com/apps.
Separation of the positional isomers of nitroaniline
Column:
Eluent:
Flow rate:
Pressure:
Detection:
Injection:
Peaks:
1. m-Nitroaniline
2. o-Nitroaniline
3. p-Nitroaniline
172
OR
RO
10
20 min
info@mn-net.com
Column:
Eluent:
Flow rate:
Pressure:
Detection:
Injection:
Column:
Eluent:
Flow rate:
Pressure:
Detection:
Injection:
O
O
CH3
N O
H 5C 2
NH
min
min
MN Appl. No. 105800
Ordering information
EC columns
NUCLEODEX -OH
4mm ID
NUCLEODEX -PM
4mm ID
NUCLEODEX -PM
4mm ID
NUCLEODEX -PM
4mm ID
Length:
200mm
EC guard
columns*
CC guard
columns**
720124.40
721171.30
721460.40
720127.40
721469.30
721464.40
720125.40
721176.30
721462.40
720752.40
721178.30
721466.40
721920
contains one CC 30/4 each with NUCLEODEX -OH, -PM, -PM and -PM as well as one CC column holder 30mm
* EC 4/3 guard columns for EC columns with 4mm ID require the Column Protection System guard column holder (REF
718966, see page 190).
** CC 8/4 guard columns for EC columns with 4mm ID require the guard column adapter EC (REF 721359, see page 191).
Columns and guard columns in packs of 1.
www.mn-net.com
173
OR
O
RO
Similar phases: Chiralcel OD, Kromasil CelluCoat, Eurocel 01, Lux Cellulose-1
High resolution type (S) with 5m particle size, allows use of shorter columns (150mm)
for faster separations, pressure stability up to ~150bar (2000psi), pH stability 19
OR
H
R=
CH3
N
O
CH3
Column:
250 x 4.6mm NUCLEOCEL DELTA S
Eluent:
n-heptane 2-propanol (90:10, v/v)
Flow rate:
1mL/min
Temperature: 25C
Detection:
UV, 254nm
Injection:
5L, 1g/L
O
= 1.29
Rs = 2.6
Column:
250 x 4.6mm NUCLEOCEL DELTA-RP S
Eluent:
acetonitrile water (40:60, v/v)
Flow rate:
0.5mL/min
Temperature: 40C
Detection:
UV, 254nm
Injection:
5L, 1g/L
10
Cl
= 1.3
Rs = 2.6
SO2NH2
HN
N
min
10
O
CH3
20 min
Ordering information
Length
EC columns
150mm
EC guard
columns*
CC guard
columns**
720445.46
4.6mm ID
4.6mm ID
250mm
721185.30
721002.40
720450.46
721186.30
721003.40
* EC 4/3 guard columns for EC columns with 4.6mm ID require the Column Protection System guard column holder (REF
718966, see page 190).
** CC 8/4 guard columns for EC columns with 4.6mm ID require the guard column adapter EC (REF 721359, see page 191).
Columns and guard columns in packs of 1
174
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Base material NUCLEOSIL silica, particle size 7m, pore size 300
chiral selector bovine serum albumin (BSA)
Separation based on selective interaction of proteins with low molecular compounds, i.e. principles of bioaffinity, including hydrophobic interactions (similar to a true reversed phase), interactions of polar groups
and steric effects
Recommended application:
Amino acid derivatives, aromatic amino acids, aromatic sulfoxides, barbiturates, benzodiazepinones, benzoin and benzoin derivatives, -blockers, coumarin derivatives, and for monitoring stereoselective microbial and enzymatic conversions
D
L
3
D
20
40
60 mL
S. Allenmark et al. in Affinity chromatography and biological recognition (I. Chaiken, M. Wilchek, and I. Parikh. Eds.), Academic Press,
New York, 1983, 259260
Column:
150 x 4mm RESOLVOSIL BSA-7
L L
Eluent:
50mmol/L phosphate buffer pH 6.5 + 1% 1-propanol
Flow rate:
0.70mL/min
Detection:
UV, 225nm
1
Peaks:
1. Serine
2
D
2. Alanine
3. Phenylalanine
Ordering information
Length:
200mm
EC guard
columns*
CC guard
columns**
720046.40
721402.30
721702.40
RESOLVOSIL BSA-7
4mm ID
* EC 4/3 guard columns for EC columns with 4mm ID require the Column Protection System guard column holder (REF
718966, see page 190).
** CC 8/4 guard columns for EC columns with 4mm ID require the guard column adapter EC (REF 721359, see page 191).
Columns and guard columns in packs of 1
www.mn-net.com
175
O H2N
*
Cu
R
O
(SiO2)n
HO
Recommended application:
Enantiomers with two polar functional groups with the correct spacing such as -amino acids,
-hydroxycarboxylic acids (e.g., lactic acid), N-alkyl--amino acids etc.
D,L-alanine enantiomers
D,L-threonine enantiomers
10 min
10 min
10 min
Ordering information
EC guard columns*
CC guard columns**
720081.40
721188.30
721455.40
NUCLEOSIL CHIRAL-1
EC columns
4mm ID
* EC 4/3 guard columns for EC columns with 4mm ID require the Column Protection System guard column holder (REF
718966, see page 190).
** CC 8/4 guard columns for EC columns with 4mm ID require the guard column adapter EC (REF 721359, see page 191).
Columns and guard columns in packs of 1
176
info@mn-net.com
enantiomer separation
in organic eluent systems
NO2
Base material NUCLEOSIL silica, particle size 5m, pore size 100
chiral selector for NUCLEOSIL CHIRAL-2 is N-(3,5-dinitrobenzoyl)D-phenylglycine, for CHIRAL-3 the optical antipode is used,
brush type phases CHIRAL-3 = USP L36
O2N
H
N
Spacer
(SiO2)n
For control of optical purity of a substance, the columns NUCLEOSIL CHIRAL-2 and NUCLEOSIL CHIRAL-3
allow to select conditions such that the minor enantiomer, present as an impurity, is eluted before the
main peak. Overlapping peaks are avoided. This makes an exact quantification of the impurity much easier.
a)
95 % D
b)
Column:
Eluent:
Flow rate:
Detection:
95 % D
O
N
OO
5% L
5% L
10 min
10 min
10
20
Columns:
Eluent:
Flow rate:
Detection:
Injection:
min
MN Appl. No. 105690
Ordering information
EC columns
NUCLEOSIL CHIRAL-2
4mm ID
NUCLEOSIL CHIRAL-3
4mm ID
Length 250mm
EC guard columns*
CC guard columns**
720088.40
721190.30
721458.40
720350.40
721190.30
721458.40
www.mn-net.com
177
(SiO2)n
Spacer
CH3
CH3
Column:
Eluent:
A260
A260
10
4
0.02
0.04
0,04
20
7S RNA
tRNA
PSTV
30
37
0
0
50
100
min
0
0
50
100
For information on DNA/RNA purification kits please ask for our catalog Bioanalysis
178
min
info@mn-net.com
plasmid
A260
0.1
supercoiled
0.04
relaxed
0
0
20
40 min
15
30 min
Ordering information
Eluent in column methanol
Length
EC columns
125mm
Guard columns
VarioPrep columns
736596.40
736400.40
736597.100
736400.40
736598
736400.40
736599.100
736400.40
736601
736400.40
736602.100
736400.40
linear
RNA
ChromCart NUCLEOGEN guard columns are 30mm long and require the CC column holder 30mm, REF 721823, see page
192 (columns in packs of 1, guard columns in packs of 2).
www.mn-net.com
179
Base material NUCLEOSIL silica, particle size 7m, pore size 4000
polymeric, covalently bonded polyethyleneimine network, weakly basic anion exchanger
ion exchange capacity 0.15mmol/g; protein binding capacity 61mg BSA/g
pH stability 28.5; max. working pressure 250bar
Separation principle: reversible adsorption of negatively charged substances to positively charged groups
on the exchanger material and their subsequent displacement by either increasing ionic strength or pH
changes in the mobile phase
High selectivity for numerous proteins; e.g., -lactoglobulins A and B, two proteins differing in just two
amino acids, can be separated in only 10 minutes; biological activity of purified proteins is preserved
Good binding and desorption kinetics for nucleotides as well
More examples for the purification of different peptides and proteins can be found in our application database at www.mn-net.com/apps
Column:
Eluent:
Flow rate:
Sample:
Recovery of proteins
Protein
Myoglobin
Transferrin
Ovalbumin
Bovine serum albumin
Glucose oxidase
-Amylase
Soybean trypsin inhibitor
-Lactoglobulin
Ferritin
Recovery [%]
100
95
98
100
100
100
100
97
85
Columns:
Eluent:
Detection:
Enzyme
Catalase (bovine liver)
L-Lactic dehydrogenase LDH-1 isoenzyme (porcine heart)
Callicrein (porcine pancreas)
Glucose oxidase (Aspergillus niger)
Peroxidase (horseradish)
Column:
Eluent:
Flow rate:
Pressure:
76bar
Detection:
UV, 280nm
Injection:
20L
Peaks:
1. Catalase
2. Myoglobin
3. a-Amylase
4. Transferrin
5. a-Lactalbumin
6. Glucose oxidase
7. Soybean trypsin inhibitor
5
4
Recovery [%]
93
102
98
104
100
10
20 min
Ordering information
Eluent in column methanol
EC colunns
Length 125mm
CC guard columns
720402.40
721091.40
CC 8/4 guard columns for EC columns with 4mm ID require the guard column adapter EC (REF 721359, see page 191).
Columns in packs of 1, guard columns in packs of 2
180
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Polymer-based strongly basic anion exchanger -N+(CH3)3, gel matrix quaternized PEI;
particle size 8m, pore size 1000 USP L23
pH working range 113, max. working pressure 200bar
Recommended application:
purification of peptides, large proteins and oligonucleotides, high capacity for proteins even at pH 10
Ordering information
Length 50mm
NUCLEOGEL SAX
Column:
Eluent:
Flow rate:
Injection:
Detection:
Peaks:
1. Conalbumin
2. Ovalbumin
3. not identified
Column:
Eluent:
min
50 x 4.6mm
NUCLEOGEL
SCX 1000-8
A) 0.02mol/L
KH2PO4, pH 6.0
B) A + 0.5mol/L
NaCl, pH 6.0
0100% B
in 20min
1mL/min
UV, 280nm
20
NUCLEOGEL SCX
Flow rate:
Detection:
Peaks:
1. Myoglobin
2. a-Chymotrypsinogen A
3. Cytochrome C
4. Lysozyme
719600
min
20
719469
4.6mm ID
Sample:
Guard columns
Polymer-based strongly acidic cation exchanger -SO3, hydrophilic gel matrix; particle size 8m,
pore size 1000 USP L22
pH working range 113, max. working pressure 200bar
Recommended application: proteins, peptides and carbohydrates with high isoelectric point
Ordering information
NUCLEOGEL SCX
Length 50mm
4.6mm ID
719475
Guard columns
NUCLEOGEL SAX and SCX Valco type guard columns measure 5 x 3mm and require the guard column holder B, REF 719539
(see page 193). Columns in packs of 1, guard columns in packs of 2
www.mn-net.com
181
Silica-based reversed phase materials with monomerically bonded alkyl chains, brush type structure
predominantly hydrophobic forces with a small portion of hydrophilic interactions
NUCLEOSIL 100-5 C18 MPN: octadecyl phase, particle size 5m, pore size 100 USP L1
dynamic protein binding capacity per g packing: 6mg BSA, 110mg cytochrome C
NUCLEOSIL 300-5 C4 MPN: butyl phase, particle size 5m, pore size 300 USP L26
dynamic protein binding capacity per g packing: 14mg BSA, 27mg cytochrome C
especially suited for the purification of larger, hydrophobic peptides and very different proteins
pH working range 28, max. working pressure 250bar
Maximum separation efficiency can be achieved when the injected protein mass does not exceed 12% of
the maximum protein loading capacity.
Column:
Eluent:
Flow rate:
Detection:
Peaks:
1. Hem
2. -globin
3. -globin
4. AT-globin
5. G-globin
6. AI-globin
20
40 min
Ordering information
Eluent in column methanol
Length 250mm
EC columns
CC guard columns
720231.40
720245.40
721113.40
CC 8/4 guard columns for EC columns with 4mm ID require the guard column adapter EC (REF 721359, see page 191).
Columns in packs of 1, guard columns in packs of 2
182
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Column:
Eluents:
Flow rate:
Detection:
Peaks:
1. Trypsin + trypsinogen
2. Proelastase
3. Lipase
+ -chymotrypsin
4. Chymotrypsinogen
5. -Amylase
6., 7. Procarboxypeptidase
30 min
0
MN Appl. No. 108220
10
Column:
Eluent:
20 min
MN Appl. No. 108280
Ordering information
Eluent in column methanol
Length
EC columns
125mm
250mm
CC guard columns
720251.40
720252.40
721594.40
720257.40
720258.40
721687.40
CC 8/4 guard columns for EC columns with 4mm ID require the guard column adapter EC (REF 721359, see page 191).
Columns in packs of 1, guard columns in packs of 2
www.mn-net.com
183
Polystyrene resin cross-linked with divinylbenzene, available particle sizes 5m and 8m, available pore
sizes 100 and 300 USP L21
pH working range 113, max. working pressure 180bar
Small pore columns for reversed phase separation of small molecules such as pharmaceuticals with basic properties, e.g., organic heterocycles; also suited for separation of nucleosides and nucleotides up to
5000Da; allow gradient as well as isocratic elution
Wide pore columns are especially recommended for large biomolecules
higher background hydrophobicity compared to silica phases
Analysis of the synthetic acyl carrier protein ACP(65-74)
Column:
Eluent:
150 x 4.6mm
NUCLEOGEL RP 100-8
A) 0.1% TFA in acetonitrile water
(1: 99, v/v); B) 0.1% TFA in
acetonitrile water (99:1, v/v)
1060% B in 20min
1mL/min
UV, 220nm
2
4
1
Flow rate:
Detection:
Peaks:
1. ACP(66-74)(H-Gln)
2. ACP(65-74)
3. ACP(66-74)(Glp)
4. Thioanisole
10
min
Ordering information
50mm
150mm
250mm
Guard columns
NUCLEOGEL RP 100-5
4.6mm ID
NUCLEOGEL RP 100-8
719456
NUCLEOGEL RP 300-5
719520
719542
NUCLEOGEL RP 300-8
4.6mm ID
719542
4.6mm ID
4.6mm ID
719455
719542
719542
Valco type guard columns measure 5 x 3mm snd require the guard column holder B, REF 719539, see page 193.
Columns in packs of 1, guard columns in packs of 2
184
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Matrix: NUCLEOSIL silica with amino modification, particle size 10m USP L8
Recommended application: RP separation of mono- and disaccharides
Separation of sugars
Column:
250 x 4mm NUCLEOSIL Carbohydrate
Eluent:
acetonitrile water (79:21, v/v)
Flow rate:
2mL/min
Temperature: 25C
Detection: RI
Injection:
10L
Peaks:
1. Fructose
2. Glucose
3. Saccharose
4. Maltose
5. Lactose
10
min
MN Appl. No. 102480
Ordering information
Length 250mm
EC guard columns*
CC guard columns**
720905.40
721170.30
721595.40
NUCLEOSIL Carbohydrate
4mm ID
* EC 4/3 guard columns for EC columns with 4mm ID require the Column Protection System guard column holder (REF
718966, see page 190).
** CC 8/4 guard columns for EC columns with 4mm ID require the guard column adapter EC (REF 721359, see page 191).
Columns and guard columns in packs of 1
www.mn-net.com
185
separation of sugars
Sulfonated polystyrene - divinylbenzene resins in different ionic forms due to a different selectivity pattern compared to NUCLEOGEL SUGAR columns, the range of application is considerably enlarged
Separation mechanism includes ion exclusion, ion exchange, size exclusion, ligand exchange as well as NP
and RP chromatography
H+ form: separation of sugars, sugar alcohols and organic acids USP L17
eluent in column 5mmol/L H2SO4
Ca2+ form: separation of mono-, di- and oligosaccharides USP L19 eluent in column water
Organic acids and alcohols
Column:
300 x 7.8mm NUCLEOGEL SUGAR 810 H
Eluent:
5mmol/L H2SO4
Flow rate:
0.6mL/min
Temperature: 35C
Detection: RI
Injection:
5L
Oxalic acid
Citric acid
Orotic acid
Maleic acid
Tartaric acid
Pyruvic acid
Malic acid
Succinic acid
Lactic acid
Formic acid
Acetic acid
Fumaric acid
Methanol
Propionic acid
Pyroglutamic acid
Ethanol
i-Butyric acid
Butyric acid
5
10
retention time [min]
15
20
25
MN Appl. No. 113870
Column:
300 x 7.8mm NUCLEOGEL SUGAR 810 Ca
Eluent:
water , flow rate 0.6mL/min
Detection: RI
Maltotriose
Raffinose
Cellobiose
Trehalose
Maltose
Sucrose
Lactose
Palatinose
Melibiose
Lactulose
Glucose
Galactose
Xylose
Sorbose
Lactitol
Maltitol
Mannose
Rhamnose
Palatitol
Fructose
Arabinose
meso-Erythritol
Mannitol
Arabitol
Xylitol
Sorbitol
Ribose
5
retention time [min]
10
15
20
MN Appl. No. 113870
Ordering information
Valco type columns
Length 300mm
Guard columns
719574
719575
719570
719571
ChromCart NUCLEOGEL SUGAR 810 guard columns measure 30 x 4mm and require the CC column holder 30mm, REF
721823, see page 192.
Columns in packs of 1, guard columns in packs of 2
186
info@mn-net.com
separation of sugars
Sulfonated spherical PS/DVB resins in different ionic forms; mean particle size 10m, pore size 100
Separation mechanism includes steric exclusion, ligand exchange and partition effects, ligand exchange
being the predominant force, since the hydrated metal ions form strong interactions with the hydroxyl
groups of the sample molecules. The intensity of these interactions decreases in the sequence Pb, Ca, Na
NUCLEOGEL ION 300 OA: H+ form for separation of sugars, alcohols and organic acids USP L17
eluent in column 5mmol/L H2SO4
NUCLEOGEL SUGAR:
Ca2+ form: separation of mono- and oligosaccharides, sugar alcohols USP L19
Pb2+ form: separation of mono- and disaccharides from food and biological samples USP L34
Na+ form: separation of oligosaccharides from starch hydrolysates and food USP L58
10
1 2
5
4
20
30 min
Separation of carbohydrates
Column:
Ordering information
Valco type columns
Length 300mm
Guard columns
7.8mm ID
719501
719537
6.5mm ID
719531
719535
7.8mm ID
719530
719534
7.8mm ID
719532
719536
NUCLEOGEL SUGAR Na
Valco-Typ guard columns measure 21 x 4mm and require the guard column holder C, REF 719538, see page 193.
Columns in packs of 1, guard columns in packs of 2
www.mn-net.com
187
C 4H 9
CH2
CH
NUCLEOGEL GPC
50
100
500
3
103
10
11
104
102
103
104
105
106
107
[MW in Da]
12
13
14
VE
VZ
V0
Exclusion volume
Dead time
Ordering information
Eluent in column toluene
Phase
Exclusion limit
[kDa]
Application
Column
300 x 7.7mm
GPC
GPC
GPC
GPC
GPC
50
100
500
103
104
GPC
GPC
GPC
GPC
GPC
50
100
500
103
104
188
2
4
25
60
500
719402
719403
719404
719405
719406
719409
2
4
25
60
500
719410
719411
719412
719413
719414
719418
info@mn-net.com
MN column systems
EC standard columns for analytical HPLC
Analytical column system manufactured from stainless steel
M8 outer threads on both ends
combination of sealing element and very fine-meshed stainless steel
screen, PTFE ring and fitting adaptor
column heads SW 12, with inner threads M8 x 0.75 and UNF 10-32
(= 1/16" connection)
ID
[mm]
2
3
4
4.6
Length [mm]
20
30
50
75
100
125
150
200
250
300
2mm
EC column with ID
3mm
4mm
4.6mm
EC
4/2
4/3
4/3
4/3
REF 718966
CC
8/3
8/3
8/4
8/4
REF 721359
Pack of
REF
EC fitting adapter
718987
718988
718992
5 kits
718998
www.mn-net.com
189
MN column systems
Column Protection System
Innovative and universal guard column holder system
suitable for all analytical HPLC columns with 1/16" fittings
REF
718966
Contents
Cartridge holder
Ferrules
Capillaries
Wrenches
Manual
Pack of
REF
Ferrules
Replacement connector including O-ring
Capillary tubes, nuts and metal ferrules
Wrench (size 12 and 14mm)
5
1
3
1
718967
718968
718969
718970
EC 4/2 UNIVERSAL RP guard column (for main columns with 2mm ID)
EC 4/2 UNIVERSAL RP guard column (for main columns with 2mm ID), value pack
3
9
728777.20
728778.20
EC 4/3 UNIVERSAL RP guard column (for main columns with 3, 4 and 4.6mm ID)
EC 4/3 UNIVERSAL RP guard column (for main columns with 3, 4 and 4.6mm ID), value pack
3
9
728777.30
728778.30
If the contaminations are colorless, replace the cartridge as soon as the pressure rises or
the chromatographic performance decreases.
190
info@mn-net.com
MN column systems
EC guard column adapter
Standard built-in guard column adapter system
suitable for EC columns
Guard column length 8mm, 3mm ID (for main columns with 2 and
3mm ID) and 4mm ID (for main columns with 4 and 4.6mm ID)
REF
721359
www.mn-net.com
Pack of
191
MN column systems
ChromCart cartridge system
Analytical column system manufactured from stainless steel
(US patent 5,342,515)
8*
125
+
+
3
4
4.6
Length [mm]
+
+
+
+
150
+
+
+
+
250
+
+
+
+
* Please note that 3mm ID guard column cartridges are also applicable for 2mm ID CC columns, and 4mm ID guard column
cartridges are also used for 4.6mm ID CC columns.
Legend
1 Analytical column
2 Sleeve
3 Guide ring
4 Nut
5 Guard column
Pack of
REF
CC connecting kit (consists of 2 nuts with end fittings, two sleeves and two guide rings)
1 kit
721690
1 set
1
1
721691
721692
721693
192
1
1
721820
721823
info@mn-net.com
MN column systems
Microbore columns
Analytical column system for rapid HPLC and LC/MS analyses with
high resolution
Available lengths: 40, 60, 100, 125, 150, 200, 250 and 300mm,
available IDs: 0.05, 0.075, 0.1, 0.15, 0.3, 0.4, 0.5, 0.75, 1, 1.5mm
Microbore columns up to 0.3mm ID are fused silica capillaries,
while microbore columns with 0.31.5mm ID are stainless steel
columns.
On request, microbore columns and guard columns can be custompacked with NUCLEODUR and NUCLEOSIL phases.
Change of flow rate and solvent saving as a
function of the column inner diameter
ID [mm]
Flow rate
[mL/min]
Solvent saving
Increase in
sensitivity
4.6
1.3
4.0
1.0
~ 25%
~ 1.3
3.0
0.56
~ 57%
~ 2.4
2.0
0.25
~ 81%
~ 5.3
1.0
0.06
~ 95%
~ 21.7
www.mn-net.com
Pack of
1
REF
719539
719538
5
5
719485
719486
193
MN column systems
VarioPrep (VP) columns for preparative HPLC
Column system for preparative HPLC, manufactured from stainless
steel with two adjustable end fittings, suitable for frequent use of
back-flushing techniques
Available standard dimensions of VarioPrep columns with axially adjustable end fittings
ID
[mm]
8
10
16
21
Length [mm]
10*
15*
32
50
40
80
50
75
+
+
+
+
100
+
+
+
+
+
+
+
Length [mm]
125
150
+
+
+
+
+
250
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
500
+
+
* 10 x 8, 10 x 16, 15 x 32 and 15 x 50mm ID columns are used as guard columns and require the respective holders, see
next page.
Dead volume
Readjustment of fitting
Column reconstitution
810th injection
just after readjustment of fitting
800 injections
750 injections
~ 100 injections
194
info@mn-net.com
MN column systems
The improved guard column system
for (semi-) preparative HPLC
Easy handling and cartridge exchange
Robust hardware
VP 10/16
for
16 and 21mm
ID columns
VP 10/8
for
8 and 10mm
ID columns
VP 15/50
for
50mm
ID columns
250
200
150
100
200
150
50
50
min
H value: 13.9
Asymmetry: 1.20
Pressure: 71bar
Peaks:
1. Phenol
2. Naphthalene
3. Anthracene
100
50
50
250
H value: 14.1
Asymmetry: 1.12
Pressure: 62bar
min
Using VarioPrep guard columns provides ideal protection of your main column symmetry, pressure and retention stay almost constant.
Technical data
1/16" thread
Guard cartridge
VP
VP
VP
VP
10/8
10/16
15/32
15/50
Holder REF
718251
718256
718253
718255
Holder ID
8mm
16mm
32mm
50mm
stainless steel
Preferred capillary ID
Columns:
125 x 16mm NUCLEODUR C18 HTec, 5m
125 x 16mm NUCLEODUR C18 HTec, 5m + 10 x 16mm NUCLEODUR C18 HTec guard column
Eluent:
acetonitrile water (80:20, v/v)
Flow rate: 16mL/min
Temperature: 22C
112mL/min
232mL/min
5150mL/min
20250mL/min
Pack of
[guard columns]
2
2
1
1
Holder
ID
REF
8mm
718251
16mm
718256
32mm
718253
50mm
718255
For REF numbers of individual VP guard column cartridges see respective NUCLEODUR and NUCLEOSIL phases.
www.mn-net.com
195
Pack of
REF
1
1
1set
1set
1set
718837
718842
718931
718852
718848
VP
VP
VP
VP
VP
1
1
1set
1set
1set
718861
718862
718853
718854
718870
OD
x
x
x
x
x
1/16"
1/16"
1/16"
1/16"
1/16"
x
x
x
x
x
ID
0.25mm
0.5mm
0.12mm
0.25mm
0.5mm
Pack of
1
1
1
1
1
coil
coil
coil
coil
coil
REF
50mm
100mm
200mm
50mm
100mm
50mm
100mm
200mm
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
1/16"
1/16"
1/16"
1/16"
1/16"
1/16"
1/16"
1/16"
1/16"
1/32"
1/32"
1/32"
1/32"
1/32"
1/32"
1/32"
1/32"
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
0.12mm
0.12mm
0.12mm
0.12mm
0.25mm
0.25mm
0.25mm
0.5mm
0.5mm
0.12mm
0.12mm
0.12mm
0.25mm
0.25mm
0.5mm
0.5mm
0.5mm
2
2
2
2
5
5
5
5
5
2
2
2
2
2
2
2
2
718731
718732
718733
718734
718588
718635
718589
718516
718517
718670
718671
718672
718673
718674
718676
718677
718678
718637/719489
718583
718737
718738
718790
718735
718736
718582
Description
Capillary accessories
1/16" column end caps (plastic)
1/16" nut for connecting 1/16" capillaries
1/16" ferrule
Capillary unions
Type 1: 100mm x 1/16" x 0.25mm
Type 2: 100mm x 1/16" x 0.12mm
Knife file
Cutter for 1/16" capillary tubing
718584
Pack of
REF
4
5
718582
718583
718584
1
1
1
1
718637
719489
706121
706290
1
1
1
1
718750
718752
718751
718753
1/16" tubing
1/16" tubing
1/8" tubing
1/8" tubing
2m frits
10m frits
2m frits
10m frits
For accessories and replacement parts for EC columns see page 189, for accessories and replacement parts for ChromCart
cartridges see page 192, for accessories and replacement parts for VarioPrep columns see page 195 and above.
196
info@mn-net.com
PEEK
O
O
C
All fittings can be tightened by hand. The following table summarizes the available PEEK products.
Ordering information
Description
REF
718770
1
1
1
718771
718772
718775
718766
718767
718768
1
1
1
1
1
718765
718760
718761
718762
718763
718769
718755
PEEK fittings
ID [mm]
0.13
0.17
0.25
0.5
0.75
www.mn-net.com
Length
1m
1m
1m
1m
1m
Pack of
197
Throughput
demands
on
preparative
separations
Yield
Purity
ID x length [mm]
Linear scale-up factor
Typical sample mass* [mg]
Typical flow rate [mL/min]
80 x 250
400
10850
200600
*For RP material; the maximum amounts given here always depend on the separation problem and on the sample composition. In some cases half of the amount given can cause drastic overload, in other cases the maximum amounts can be even
higher still giving acceptable separations.
Pore size 110, pore volume 0.9mL/g, surface (BET) 340m2/g, density 0.47g/mL, pressure stability
600bar
Larger particles for preparative applications
Ordering information
Phase
Endcapped
Carbon content
Particle size
Pack of 100g
Pack of 1000g
yes
yes
yes
18% C
18% C
18% C
5m
7m
10m
713830.0100
713831.0100
713832.0100
713830.1
713831.1
713832.1
NUCLEODUR
NUCLEODUR
NUCLEODUR
NUCLEODUR
NUCLEODUR
NUCLEODUR
100-10
100-12
100-16
100-20
100-30
100-50
yes
yes
yes
yes
yes
yes
10m
12m
16m
20m
30m
50m
713611.0100
713618.0100
713621.0100
713601.0100
713631.0100
713550.0100
713611.1
713618.1
713621.1
713601.1
713631.1
713550.1
NUCLEODUR
NUCLEODUR
NUCLEODUR
NUCLEODUR
NUCLEODUR
NUCLEODUR
100-10
100-12
100-16
100-20
100-30
100-50
10m
12m
16m
20m
30m
50m
713610.0100
713615.0100
713620.0100
713600.0100
713630.0100
713551.0100
713610.1
713615.1
713620.1
713600.1
713630.1
713551.1
ec
ec
ec
ec
ec
ec
17.5%
17.5%
17.5%
17.5%
17.5%
17.5%
C
C
C
C
C
C
198
info@mn-net.com
50
100
120
300
500
1000
4000
50
100
120
300
500
1000
4000
Pore volume
0.8mL/g
1mL/g
0.65mL/g
0.8mL/g
0.8mL/g
0.8mL/g
0.7mL/g
Surface (BET)
420m2/g
350m2/g
200m2/g
100m2/g
35m2/g
25m2/g
10m2/g
Density
0.45g/mL
0.36g/mL
0.55g/mL
0.45g/mL
0.45g/mL
0.45g/mL
0.48g/mL
Pressure stability*
500bar
500bar
500bar
400bar
400bar
300bar
300bar
For description of individual modifications see chapter Columns with NUCLEOSIL from page 157.
* Maximum packing pressure of NUCLEOSIL bulk packings
Ordering information
Phase
Endcapped
Carbon content
Pore size
Particle size
Pack of 10g
Octadecyl phases
(CH2)17CH3
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
50-5 C18 ec
100-5 C18 AB
100-3 C18
100-5 C18
100-7 C18
100-10 C18
120-3 C18
120-5 C18
120-7 C18
120-10 C18
300-5 C18
300-7 C18
300-10 C18
500-7 C18
1000-7 C18
4000-7 C18
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
14.5%
24%
15%
15%
15%
15%
11%
11%
11%
11%
6.5%
6.5%
6.5%
2%
~ 1%
<1%
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
50
100
100
100
100
100
120
120
120
120
300
300
300
500
1000
4000
5m
5m
3m
5m
7m
10m
3m
5m
7m
10m
5m
7m
10m
7m
7m
7m
712031.10
712952.10
712370.10
712130.10
712140.10
712150.10
712460.10
712470.10
712480.10
712490.10
712520.10
712530.10
712540.10
712760.10
712790.10
712926.10
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
50-5 C8 ec
100-5 C8 ec
100-5 C8
100-7 C8
100-10 C8
120-3 C8
120-5 C8
120-7 C8
120-10 C8
300-5 C8
300-7 C8
300-10 C8
500-7 C8
yes
yes
no
no
no
no
no
no
no
no
no
no
no
9%
9%
8.5%
8.5%
8.5%
6.5%
6.5%
6.5%
6.5%
~ 3%
~ 3%
~ 3%
<1%
C
C
C
C
C
C
C
C
C
C
C
C
C
50
100
100
100
100
120
120
120
120
300
300
300
500
5m
5m
5m
7m
10m
3m
5m
7m
10m
5m
7m
10m
7m
712032.10
712101.10
712100.10
712110.10
712120.10
712570.10
712580.10
712500.10
712590.10
712650.10
712550.10
712660.10
712830.10
Octyl phases
www.mn-net.com
Pack of 100g
712031.100
712952.100
712370.100
712130.100
712140.100
712150.100
712460.100
712470.100
712480.100
712490.100
712520.100
712530.100
712540.100
712760.100
712790.100
712926.100
(CH2)7CH3
712032.100
712101.100
712100.100
712110.100
712120.100
712570.100
712580.100
712500.100
712590.100
712650.100
712550.100
712660.100
712830.100
Phase
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
199
Endcapped
Carbon content
Pore size
Particle size
Pack of 10g
Phenyl phases
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
100-5 C6H5 ec
100-5 C6H5
100-7 C6H5
120-7 C6H5
300-7 C6H5
500-7 C6H5
1000-7 C6H5
Butyl phases
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
120-5 C4
300-5 C4
300-7 C4
300-10 C4
500-7 C4
1000-7 C4
4000-7 C4
Dimethyl phases
NUCLEOSIL 100-7 C2
(CH2)3C6H5
yes
no
no
no
no
no
no
yes
yes
yes
yes
yes
yes
yes
no
100-5 CN
100-10 CN
120-7 CN
300-7 CN
500-7 CN
Diol phases
100-7 OH (Diol)
300-7 OH (Diol)
500-7 OH (Diol)
1000-7 OH (Diol)
4000-7 OH (Diol)
Amino phases
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
100-5 NH2
100-10 NH2
120-7 NH2
300-7 NH2
Dimethylamino phases
NUCLEOSIL 100-5 N(CH3)2
NUCLEOSIL 100-10 N(CH3)2
C
C
C
C
C
C
C
~ 4%
~ 2%
~ 2%
~ 2%
<1%
<1%
<1%
C
C
C
C
C
C
C
3.5% C
NUCLEOSIL 100-5 SA
NUCLEOSIL 100-10 SA
200
5m
5m
7m
7m
7m
7m
7m
712311.10
712310.10
712340.10
712510.10
712670.10
712923.10
712924.10
712311.100
712310.100
712340.100
712510.100
712670.100
712923.100
712924.100
120
300
300
300
500
1000
4000
5m
5m
7m
10m
7m
7m
7m
712290.10
712620.10
712630.10
712640.10
712750.10
712780.10
712925.10
712290.100
712620.100
712630.100
712640.100
712750.100
712780.100
712925.100
100
7m
712080.10
712080.100
(CH2)3CN
5%
5%
~ 3%
~ 2.5%
~ 2%
C
C
C
C
C
100
100
120
300
500
5m
10m
7m
7m
7m
712160.10
712170.10
712600.10
712820.10
712840.10
712160.100
712170.100
712600.100
712820.100
712840.100
(CH2)3C6H4NO2
~ 4.5% C
~ 4.5% C
100
100
5m
10m
712180.10
712190.10
712180.100
712190.100
(CH2)3OCH2CH(OH)CH2OH
5% C
~ 1.5% C
~ 1.5% C
~ 1% C
~ 1% C
100
300
500
1000
4000
7m
7m
7m
7m
7m
712350.10
712560.10
712740.10
712770.10
712927.10
712350.100
712560.100
712740.100
712770.100
712927.100
(CH2)3NH2
3.5%
3.5%
~ 2%
~ 2%
C
C
C
C
100
100
120
300
5m
10m
7m
7m
712200.10
712210.10
712610.10
712919.10
712200.100
712210.100
712610.100
712919.100
(CH2)3N(CH3)2
4% C
4% C
100
100
100
120
300
500
1000
(CH3)2
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
8%
8%
8%
6.5%
~ 3%
~ 2%
~ 1%
(CH2)3CH3
Nitro phases
Pack of 100g
6.5% C
6.5% C
100
100
5m
10m
712220.10
712230.10
712220.100
712230.100
(CH2)3C6H4SO3 Na
100
100
5m
10m
712240.10
712250.10
712240.100
712250.100
info@mn-net.com
Endcapped
Carbon content
Pore size
Particle size
10% C
10% C
Pack of 10g
(CH2)3C6H4CH2N+(CH3)3Cl
100
100
712260.10
712270.10
5m
10m
Unmodified silica
712260.100
712270.100
SiOH
50-3
50-5
50-7
50-10
100-3
100-5
100-7
100-10
120-3
120-5
120-7
120-10
300-5
300-7
300-10
500-5
500-7
500-10
1000-5
1000-7
1000-10
4000-5
4000-7
4000-10
50
50
50
50
100
100
100
100
120
120
120
120
300
300
300
500
500
500
1000
1000
1000
4000
4000
4000
712000.10
712010.10
712020.10
712030.10
712360.10
712040.10
712050.10
712060.10
712390.10
712400.10
712410.10
712420.10
712430.10
712440.10
712450.10
712680.10
712690.10
712700.10
712710.10
712720.10
712730.10
712850.10
712860.10
712870.10
3m
5m
7m
10m
3m
5m
7m
10m
3m
5m
7m
10m
5m
7m
10m
5m
7m
10m
5m
7m
10m
5m
7m
10m
712000.100
712010.100
712020.100
712030.100
712360.100
712040.100
712050.100
712060.100
712390.100
712400.100
712410.100
712420.100
712430.100
712440.100
712450.100
712680.100
712690.100
712700.100
712710.100
712720.100
712730.100
712850.100
712860.100
712870.100
Pore size
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
60
100
300
1000
60
100
300
1000
Pore volume
0.75mL/g
1mL/g
0.8mL/g
0.8mL/g
Surface (BET)
350m2/g
280m2/g
100m2/g
25m2/g
Density
Pressure stability
0.45g/mL
0.35g/mL
0.45g/mL
0.45g/mL
600bar
400bar
400bar
300bar
www.mn-net.com
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
NUCLEOSIL
Pack of 100g
201
Endcapped
Carbon content
Pore size
Particle size
Pack of 10g
Octadecyl phases
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
60-5 C18
60-7 C18
60-10 C18
100-5 C18
100-7 C18
100-10 C18
300-7 C18
1000-7 C18
POLYGOSIL 60-5 C8
POLYGOSIL 60-7 C8
POLYGOSIL 60-10 C8
yes
yes
yes
yes
yes
yes
yes
yes
12%
12%
12%
14%
14%
14%
4%
~ 1%
C
C
C
C
C
C
C
C
60
60
60
100
100
100
300
1000
5m
7m
10m
5m
7m
10m
7m
7m
711330.10
711340.10
711350.10
711560.10
711570.10
711580.10
711710.10
711992.10
POLYGOSIL 300-7 C4
POLYGOSIL 1000-7 C4
711330.100
711340.100
711350.100
711560.100
711570.100
711580.100
711710.100
711992.100
(CH2)7CH3
no
no
no
7% C
7% C
7% C
60
60
60
5m
7m
10m
711300.10
711310.10
711320.10
yes
yes
~ 1% C
<1% C
300
1000
7m
7m
711680.10
711991.10
~ 5% C
~ 5% C
60
60
5m
10m
711380.10
711390.10
~ 4.5% C
~ 4.5% C
60
60
5m
10m
711400.10
711410.10
Butyl phases
711300.100
711310.100
711320.100
(CH2)3CH3
711680.100
711991.100
(CH2)3CN
Nitro phases
711380.100
711390.100
(CH2)3C6H4NO2
711400.100
711410.100
Unmodified silica
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
POLYGOSIL
SiOH
60-5
60-7
60-10
100-5
100-7
100-10
300-7
1000-7
60
60
60
100
100
100
300
1000
5m
7m
10m
5m
7m
10m
7m
7m
711010.10
711280.10
711020.10
711510.10
711520.10
711530.10
711600.10
711890.10
Amino phases
POLYGOSIL 60-5 NH2
POLYGOSIL 60-10 NH2
711010.100
711280.100
711020.100
711510.100
711520.100
711530.100
711600.100
711890.100
(CH2)3NH2
~ 3% C
~ 3% C
60
60
5m
10m
Dimethylamino phases
202
(CH2)17CH3
Octyl phases
Pack of 100g
711360.10
711370.10
711360.100
711370.100
(CH2)3N(CH3)2
~ 3.5% C
~ 3.5% C
60
60
5m
10m
711420.10
711430.10
711420.100
711430.100
info@mn-net.com
Pore size
60
100
300
1000
60
100
300
1000
Pore volume
0.75mL/g
1mL/g
0.8mL/g
0.8mL/g
Surface (BET)
350m2/g
280m2/g
100m2/g
35m2/g
Density
0.45g/mL
0.35g/mL
0.45g/mL
0.45g/mL
Pressure stability
600bar
400bar
400bar
300bar
Ordering information
Phase
Octadecyl phases
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
60-12 C18
60-20 C18
60-30 C18
60-50 C18
60-80 C18
60-130 C18
100-12 C18
100-20 C18
100-30 C18
100-50 C18
300-12 C18
300-20 C18
300-30 C18
300-50 C18
1000-30 C18
1000-50 C18
Octyl phases
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
60-12
60-20
60-30
60-50
C8
C8
C8
C8
Butyl phases
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
Endcapped
Carbon content
Pore size
Particle size
Pack of 100g
(CH2)17CH3
no*
no*
no*
no*
no*
no*
no*
no*
no*
no*
yes
yes
yes
yes
yes
yes
12%
12%
12%
12%
12%
12%
14%
14%
14%
14%
4%
4%
4%
4%
~ 1%
~ 1%
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
60
60
60
60
60
60
100
100
100
100
300
300
300
300
1000
1000
1015m
1525m
2540m
4063m
63100m
63200m
1015m
1525m
2540m
4063m
1015m
1525m
2540m
4063m
2540m
4063m
711009.100
711031.100
711480.100
711500.100
711011.100
711590.100
711018.100
711019.100
711032.100
711021.100
711024.100
711025.100
711720.100
711730.100
711028.100
711029.100
no*
no*
no*
no*
7%
7%
7%
7%
C
C
C
C
60
60
60
60
1015m
1525m
2540m
4063m
711007.100
711008.100
711470.100
711490.100
711007.1000
711008.1000
711470.1000
711490.1000
(CH2)3CH3
yes
yes
yes
yes
yes
yes
~
~
~
~
<
<
1%
1%
1%
1%
1%
1%
C
C
C
C
C
C
300
300
300
300
1000
1000
1015m
1525m
2540m
4063m
2540m
4063m
711022.100
711023.100
711690.100
711700.100
711026.100
711027.100
www.mn-net.com
711009.1000
711031.1000
711480.1000
711500.1000
711011.1000
711590.1000
711018.1000
711019.1000
711032.1000
711021.1000
711024.1000
711025.1000
711720.1000
711730.1000
711028.1000
711029.1000
(CH2)7CH3
300-12 C4
300-20 C4
300-30 C4
300-50 C4
1000-30 C4
1000-50 C4
POLYGOPREP 60-12 CN
POLYGOPREP 60-20 CN
POLYGOPREP 60-30 CN
Pack of 1kg
711022.1000
711023.1000
711690.1000
711700.1000
711026.1000
711027.1000
POLYGOPREP
POLYGOPREP
POLYGOPREP
POLYGOPREP
(CH2)3CN
~ 4.5% C
~ 4.5% C
~ 4.5% C
60
60
60
1015m
1525m
2540m
711015.100
711016.100
711017.100
711015.1000
711016.1000
711017.1000
203
Amino phases
Endcapped
Carbon content
~ 3% C
~ 3% C
~ 3% C
Pore size
60-12
60-20
60-30
60-50
60-80
60-130
100-12
100-20
100-30
100-50
100-80
100-130
300-12
300-20
300-30
300-50
1000-12
1000-20
1000-30
1000-50
Particle size
Pack of 100g
Pack of 1kg
(CH2)3NH2
Pore size
60
60
60
60
60
60
100
100
100
100
100
100
300
300
300
300
1000
1000
1000
1000
Particle size
60
60
60
1015m
1525m
2540m
711012.100
711013.100
711014.100
711012.1000
711013.1000
711014.1000
Pack of 100g
Pack of 1kg
Pack of 5kg
711004.100
711610.100
711620.100
711630.100
711035.100
711036.100
711005.100
711006.100
711001.1000
711240.1000
711250.1000
711260.1000
711270.1000
711037.1000
711002.1000
711003.1000
711540.1000
711550.1000
711033.1000
711034.1000
711004.1000
711610.1000
711620.1000
711630.1000
711035.1000
711036.1000
711005.1000
711006.1000
711001.5000
711240.5000
711250.5000
711260.5000
711270.5000
711037.5000
711002.5000
711003.5000
711540.5000
711550.5000
711033.5000
711034.5000
SiOH
1015m
1525m
2540m
4063m
63100m
63200m
1015m
1525m
2540m
4063m
63100m
63200m
1015m
1525m
2540m
4063m
1015m
1525m
2540m
4063m
Silica FIA for the fluorescence indicator adsorption procedure for the
determination of hydrocarbon groups in the testing of liquid fuels in
accordance with DIN 51791 and ASTM D 1319-58T
The FIA method determines saturated hydrocarbons, olefins and aromatic hydrocarbons of a sample chromatographically by adsorption
and desorption in a column filled with FIA silica, in the presence of a
fluorescent dye mixture.
info@mn-net.com
60, 0.0150.04mm
60, 0.0250.04mm
60, 0.040.063mm
60 M, 0.040.063mm
60, 0.050.1mm
60, 0.050.2mm
60, 0.0630.2mm
60, < 0.063mm
60, < 0.08mm
60, 0.10.2mm
60, 0.20.5mm
60, 0.51.0mm
FIA fine
FIA coarse
Particle size
1kg
5kg
815650.1
815300.1
815380.1
815381.1
815390.1
815320.1
815330.1
815400.1
815310.1
815340.1
815350.1
815360.1
815410.1
815430.1
230400mesh
230400mesh
130270mesh
70270mesh
70230mesh
+230mesh
+190mesh
70130mesh
3570mesh
1835mesh
0.0710.16mm
0.0710.63mm
815650.5
815300.5
815380.5
815381.5
815390.5
815320.5
815330.5
815400.5
815310.5
815340.5
815350.5
815360.5
25kg
815650.25
815300.25
815380.25
815381.25
815390.25
815320.25
815330.25
815400.25
815310.25
815350.25
815360.25
Aluminium oxide
Aluminium oxides produced by dehydration of different aluminium hydroxides, e.g., hydrargillite between
400 and 500C
Activity grade I, particle size 50200m, specific surface (BET) ~ 130m2/g
Ordering information
Type
Aluminium oxide 90 basic
Aluminium oxide 90 neutral
Aluminium oxide 90 acidic
pH
pH 9.5 0.3
pH 7 0.5
pH 4 0.3
1kg
815010.1
815020.1
815030.1
5kg
815010.5
815020.5
815030.5
25kg
815010.25
815020.25
815030.25
Kieselguhr
Naturally occurring amorphous silicic acids of fossil origin, also known as diatomaceous earth or diatomite
purified for chromatographic applications
Compared to silica, kieselguhr has a small surface of low activity application in partition chromatography; impregnated with various substances (paraffin, silicone oil, undecane) it can be used for reversed
phase chromatography
The following grades of kieselguhr are manufactured by Johns-Manville. They are narrowly classified with
homogeneous particle size distributions and high purity.
For columns packed with kieselguhr please see CHROMABOND XTR for liquid-liquid extraction, page 56.
Ordering information
Designation
Filter-Cel
Hyflo Super-Cel
Celite 503
Celite 535
Celite 545
www.mn-net.com
1kg
815510.1
815530.1
815540.1
815550.1
815560.1
5kg
815510.5
815530.5
815540.5
815550.5
815560.5
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
205
Hard granular magnesia silica gel: MgO 15.50.5% SiO2 84.00.5% Na2SO4 1.0%; 60/100mesh
Typical applications: sample preparation (see chapter Solid phase extraction, page 34); clean-up of
pesticide residues, separation of chlorinated pesticides, extraction of steroids, sex hormones, antibiotics,
lipids etc.
Ordering information
Designation
Florisil standard 60/100mesh
Particle size
0.15/0.25mm
1kg
815710.1
5kg
815710.5
Polyamide
Polyamide 6 = -aminopolycaprolactam
separation mechanism mainly based on hydrogen bonds
For SPE columns packed with polyamide see CHROMABOND PA page 34.
Ordering information
Designation
Polyamide CC 6, < 0.07mm
Polyamide CC 6, 0.050.16mm
Polyamide CC 6, 0.100.30mm
Particle size
< 0.07mm
0.050.16mm
0.100.30mm
1kg
815610.1
815620.1
815600.1
Unmodified cellulose
Cellulose MN 100: native fibrous cellulose, standard grade
average degree of polymerization 620680, fiber length (85%) 20100m,
specific surface acc. to Blaine ~ 6500cm2/g; residue on ignition at 850C < 10000ppm,
< 20ppm Fe, < 5ppm Cu, < 7ppm P, CH2Cl2 extract < 0.20%
Cellulose MN 2100: native fibrous cellulose, purified grade (washed with different eluents)
average degree of polymerization 620680, fiber length (85%) 2075m,
specific surface acc. to Blaine ~ 5500cm2/g
residue on ignition at 850C < 1000ppm, < 2ppm Fe, < 1ppm Cu, < 2ppm P, CH2Cl2 extract < 0.15%
Grade MN 2100ff is a defatted cellulose MN 2100 with a CH2Cl2 extract < 0.02%
Ordering information
206
Designation
Cellulose MN 100
Cellulose MN 2100
Cellulose MN 2100ff (cellulose MN 2100 defatted)
1kg
815050.1
815060.1
815070.1
5kg
815050.5
815060.5
25kg
815050.25
815060.25
info@mn-net.com
TLC Contents
Basic principles of TLC
208
211
216
213
219
224
Cellulose layers
225
226
Chromatography papers
229
TLC micro-sets
Introductory kits for TLC
230
Visualization reagents
233
233
Glass plates
www.mn-net.com
234
207
Separated substances can be subjected to subsequent analytical procedures (e.g., IR, MS) at a later
date
Rapid and cost-efficient optimization of the separation due to easy change of mobile and stationary
phase
For a better understanding of a thin layer chromatographic separation we describe here the basic steps:
Sample preparation
Sample application
For separation the sample must meet several requirements to obtain good results. Since the TLC plate is a
disposable product, sample preparation in general is
not as demanding as for other chromatographic methods. However, eventually several steps for sample pretreatment may be necessary. These include sampling,
mechanical crushing, extraction steps, filtration and
sometimes enrichment of interesting components or
clean-up, i.e. removal of undesired impurities.
Sample application
208
silica layer
concentrating
zone
arbitrary application
in the conc. zone
developed
chromatogram
The most frequently used separation technique is ascending TLC in a trough chamber (standard method,
linear development). Usually it is applied as single development. However, multiple development, with or
without change of eluent (step technique) can improve
separation results. For 2-dimensional development only
1 spot of the sample is applied in one edge of a plate.
After chromatography in the first direction the plate is
dried, turned by 90 and developed in the 2nd dimension with another eluent. Thus complicated mixtures
give 2-dimensional chromatograms taking advantage
of the different separating properties of two eluents.
For selection and optimization of the eluent numerous publications are available. A generally applicable
standardized optimization method is described by H.
Keuker et al. [in Proceedings of the International Symposium on
Support with
adsorbent layer
Solvent
vapor
Filter paper
Eluent
A) normal saturation, arrows
show evaporation of the
eluent from the layer
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Evaluation depends on the purpose of the chromatographic analysis. For qualitative determination often localization of substances is sufficient. This can be easily
achieved by parallel runs with reference substances.
A parameter often used for qualitative evaluation is the
Rf value (retention factor) or the 100fold value hRf. The
Rf value is defined as follows:
distance starting line middle of spot
Rf =
b
a
Quantitative evaluation is possible by suitable calibration measurements. For this purpose either the area of
a substance spot is measured or a photometric evaluation is performed directly on the layer. The latter procedure, however, requires a higher instrumental expense.
The following paragraphs describe the most frequently
used methods for evaluation in TLC.
Solvent front
Substance spot
a
b
Starting line
209
210
Quantitative evaluation
Classical adsorbents
for ~80% of all TLC separations silica 60 (mean
pore diameter 60 = 6nm) is used. Other classical
adsorbents are aluminium oxide, cellulose, kieselguhr, ion exchangers and polyamide.
Special phases
reversed phases, mainly C18 (octadecyl) modified
silica, but also cyano, amino, diol and RP-2 modified silica. Special layers for specific separations,
like our CHIRALPLATE for enantiomer separation,
complete the versatile range of TLC plates.
POLYGRAM
ALUGRAM/ALUGRAM Xtra
Material
Glass
Thickness (approx.)
1.3mm
Weight, packaging and storage requirements high
Torsional strength
ideal
Temperature stability
high
Susceptible to breakage
yes
Can be cut with scissors
no
Polyester
0.2mm
low
low
max. 185C
no
yes
Aluminium
0.15mm
low
relatively high
high
no
yes
against solvents
against mineral acids and conc. ammonia
high
high
high
high
high
low
depending on
phase
very suitable
ALUGRAM: limited
suitability; ALUGRAM Xtra:
very suitable
www.mn-net.com
211
Support*
Standard silica
ADAMANT
DURASIL
G P A Ax
SIL G
SILGUR
Ax
Ax
Nano-SIL
A Ax
Nano-ADAMANT
Nano-DURASIL
RP-2/UV254
Nano-SIL CN
Nano-SIL NH2
Nano-SIL DIOL
Aluminium oxide
Alox-25/Alox N
G P A
Layer
Page
213
215
216
216
218
219
220
217
G P A
G P
CEL 400
CEL 300 AC
CHIRALPLATE
SIL N-HR
SIL G-25 HR
Nano-SIL PAH
Alox/CEL-AC-Mix
SILCEL-Mix
G = glass plates
212
218
220
221
222
Aluminium oxide
224
223
215
225
225
226
Perlon = -aminopolycaprolactame
226
226
226
High purity silica 60, special binder system, higher gypsum content
227
228
228
High purity silica 60 with gypsum, recommended for aflatoxin analysis 227
Nano silica with special impregnation for PAH analysis
info@mn-net.com
228
228
229
229
Silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60, specific pore volume 0.75mL/g,
particle size 517m
Outstanding hardness and abrasion resistance due to an optimized binder system
Increased separation efficiency due to an optimized particle size distribution
High suitability for trace analyses resulting from a UV indicator with increased brilliance and a lownoise background of the layer
Separation of steroids
Separation of barbiturates
ADAMANT UV254
Rf
Cortisone
Corticosterone
Testosterone
Deoxycorticosterone
Progesterone
Migration distance
Layer:
ADAMANT UV254
Eluent:
chloroform acetone (95:5, v/v)
Migration distance: 73mm in 20min
Sample volume: 1L
Detection: UV
SIL G/UV254
ADAMANT
0.37
0.43
0.50
0.55
0.73
5.0cm
SIL G
0.27
0.30
0.39
0.46
0.62
5.7cm
Substance
Thiamylal (0.5%)
Thiopental (1.0%)
Hexobarbital (5.0%)
Pentobarbital (1.0%)
Phenobarbital (1.0%)
Rf
0.69
0.65
0.41
0.26
0.18
MN Appl. No. 402950
Layers:
ADAMANT UV254, SIL G/UV254
Eluent:
chloroform methanol (97:3, v/v)
Developing time: 10min
Sample:
0.1% solution in CHCl3
Detection: UV
For more applications of ADAMANT ready-to-use layers, check our application database at www.mn-net.com/apps.
Ordering information
Plate size [cm] 2.5 x 7.5
Pack of [plates]
100
Glass plates
ADAMANT
ADAMANT UV254
821005
www.mn-net.com
50
821040
821010
5 x 10
200
5 x 20
100
10 x 10
25
10 x 20
50
20 x 20
25
Thickness Fluorescent
of layer
indicator
821040.200
821010.200
821015
821050
821020
821025
821060
821030
0.25mm
0.25mm
UV254
213
Silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60, specific pore volume 0.75mL/g,
particle size 517m; standard grade
Outstanding wettability for precise colorization results, even with 100% aqueous detection reagents
Excellent separation efficiency and reproducibility from lot to lot
Easy and reliable cutting due to an optimized binder system, no flaking of silica
Binder: highly polymeric product, which is stable in almost all organic solvents and resistant towards aggressive visualization reagents; also completely stable in purely aqueous eluents
Anethole
Myristicin
Eugenol
Linalool
Sample:
Ordering information
Plate size [cm] 2.5 x 7.5
Pack of [plates]
200
4x8
50
5 x 7.5
20
5 x 10
50
5 x 20
50
10 x 20
20
20 x 20
25
Thickness Fluorescent
of layer
indicator
818331
818230.20
818330.20
818261
818360
818232
818332
818362
818233
818333
0.20mm
0.20mm
214
818329
info@mn-net.com
UV254
Silica 60, specific surface (BET) ~ 500m /g, mean pore size 60, specific pore volume 0.75mL/g,
particle size 517m; standard grade
2
Thickness of layer for analytical plates 0.25mm, for preparative plates 0.5 and 1mm;
for 2mm preparative layers a slightly coarser material is used
Indicators: manganese activated zinc silicate with green fluorescence for short-wave UV (254nm); special
inorganic fluorescent pigment with blue fluorescence for long-wave UV (366nm)
Binders: highly polymeric products, which are stable in almost all organic solvents and resistant towards
aggressive visualization reagents; binder system for POLYGRAM sheets (as for ALUGRAM Xtra sheets) is
also completely stable in purely aqueous eluents
Glass plates
SIL
SIL
SIL
SIL
SIL
SIL
SIL
SIL
SIL
2.5 x 7.5
100
809028.100
5 x 10
50
200
809017
809017.200
809027
809027.200
(preparative TLC)
5 x 20
100
809011
809021
809121
10 x 10
25
809020
10 x 20
50
809012
809022
809122
(preparative TLC)
(preparative TLC)
2.5 x 7.5
200
805902
805901
4x8
50
805032
805021
2.5 x 7.5
200
4x8
50
818129
818131
5 x 20
50
805012
805022
DURASIL
5 x 7.5
20
818030.20
818130.20
5 x 10
50
818161
818160
5 x 20
50
818032
818132
20 x 20
25
809013
809023
809123
20
809051
809053
15
809061
809063
12
809073
809083
Thickness
of layer
0.25mm
0.25mm
0.25mm
0.50mm
0.50mm
1.00mm
1.00mm
2.00mm
2.00mm
20 x 20
25
805013
805023
40 x 20
25
805014
805024
805017
0.20mm
0.20mm
0.20mm
10 x 20
20
818163
818162
20 x 20
25
818033
818133
0.20mm
0.20mm
Ordering information
Silica 60, specific surface (BET) ~ 500m /g, mean pore size 60, specific pore volume 0.75mL/g,
particle size 517m
2
Ordering information
Plate size [cm]
Pack of [plates]
Glass plates
DURASIL-25
DURASIL-25 UV254
50
5 x 10
812005
www.mn-net.com
200
5 x 20
100
10 x 20
50
20 x 20
25
Thickness of
layer
Fluorescent
indicator
812005.200
812006
812003
812007
812004
812008
0.25mm
0.25mm
UV254
215
Silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60, specific pore volume 0.75mL/g,
particle size 517m
Kieselguhr zone for rapid sample application: because kieselguhr is completely inert towards a large
number of compounds, the samples always form a narrow band at the interface of the two adsorbents,
irrespective of shape, size or position of the spots in the concentrating zone (see page 208). Separation
then takes place in the silica layer.
Available as glass plate and as ALUGRAM Xtra aluminium sheet with or without fluorescent indicator (for
advantages of ALUGRAM Xtra see page 214)
Ordering information
Plate size [cm]
10 x 20
20 x 20
Thickness of layer
Fluorescent indicator
Pack of [plates]
SILGUR-25
SILGUR-25 UV254
50
810012
810022
25
810013
810023
0.25mm
0.25mm
UV254
Pack of [plates]
SILGUR
SILGUR UV254
20
818412
818422
25
818413
818423
0.20mm
0.20mm
UV254
arbitrary application in
the concentrating zone
applied substances
are concentrated
applied substances at
the phase boundary
developed
chromatogram
Glass plates
Silica layer
Concentrating zone
Nano-SILGUR
Nano silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60,
specific pore volume 0.75mL/g, particle size 210m
Narrow fractionation of the silica for sharper separations, shorter developing times, shorter migration distances, lower amount of samples and an increased detection sensitivity compared to SILGUR plates
Kieselguhr zone for rapid sample application (see SILGUR above)
Ordering information
Plate size 10 x 10cm, pack of 25 plates
Thickness of layer
Fluorescent indicator
Nano-SILGUR-20
Nano-SILGUR-20 UV254
811032
811042
0.20mm
0.20mm
UV254
Nano-SILGUR
Nano-SILGUR UV254
818432
818442
0.20mm
0.20mm
UV254
Glass plates
216
info@mn-net.com
Nano silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60, specific pore volume 0.75mL/g,
particle size 210m
Outstanding hardness and abrasion resistance due to an optimized binder system
Increased separation efficiency due to an optimized particle size distribution
High suitability for trace analyses resulting from a UV indicator with increased brilliance and a lownoise background of the layer
A) ADAMANT
B) Nano-ADAMANT
1L, about 0.1%
toluene cyclohexane (4:3, v/v)
A) 30min, B) 15min
2
3
10
20
30
40
50
mm
1
2
3
10
20
30
40 mm
Ordering information
Glass plates
Nano-ADAMANT
Nano-ADAMANT UV254
www.mn-net.com
10 x 10
25
10 x 20
50
Thickness of layer
Fluorescent
indicator
821140
821110
821150
821120
0.20mm
0.20mm
UV254
Narrow fractionation of the silica particles allows theoretical plate heights, which are one order of magnitude smaller than on standard silica layers with the advantage of sharper separations, shorter developing times, shorter migration distances, lower amount of samples, and increased detection sensitivity with
equal selectivity.
217
Nano silica 60, specific surface (BET) ~ 500m /g, mean pore size 60, specific pore volume 0.75mL/g,
particle size 210m
indicator: manganese activated zinc silicate with green fluorescence for short-wave UV (254nm)
binder: highly polymeric product, which is stable in almost all organic solvents and resistant towards aggressive visualization reagents
2
Narrow fractionation of the silica particles allows sharper separations, shorter developing times, shorter
migration distances, smaller samples and an increased detection sensitivity compared to SIL G plates.
Available as glass plate and as ALUGRAM Xtra aluminium sheet with or without fluorescent indicator (advantages of ALUGRAM Xtra see page 214)
Ordering information
Plate size [cm]
Pack of [plates]
Glass plates
Nano-SIL-20
Nano-SIL-20 UV254
5x5
100
5 x 20
50
811011
811021
10 x 10
25
10 x 20
50
811012
811022
811013
811023
Nano-SIL G
Nano-SIL G/UV254
818240
818342
Nano-SIL G
Nano-SIL G/UV254
Nano-DURASIL
20 x 20
25
Thickness of layer
Fluorescent
indicator
0.20mm
0.20mm
UV254
818241
818343
0.20mm
0.20mm
UV254
818141
818143
0.20mm
0.20mm
UV254
Nano silica 60, specific surface (BET) ~ 500m /g, mean pore size 60, specific pore volume 0.75mL/g,
particle size 210m
indicator: manganese activated zinc silicate with green fluorescence for short-wave UV (254nm)
hard, water-resistant and wettable layers due to a special binder system
2
Narrow fractionation of the silica particles allows sharper separations, shorter developing times, shorter
migration distances, smaller samples and an increased detection sensitivity compared to DURASIL plates
different selectivity compared to ADAMANT and SIL-G plates
no reversed phase tendency, more polar than Nano-SIL
Ordering information
Glass plates
Nano-DURASIL-20
Nano-DURASIL-20 UV254
218
10 x 10
25
10 x 20
50
Thickness of layer
Fluorescent
indicator
812010
812013
812011
812014
0.20mm
0.20mm
UV254
info@mn-net.com
Base material:
Nano silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60,
specific pore volume 0.75mL/g, particle size 210m, pH stability 210
Indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254nm); UV-absorbing substances appear as dark-blue to black spots on a light-blue background
Partial (50%) or complete (100%) octadecyl modification, carbon content 7.5 and 14%, respectively
Order of polarity: silica > DIOL > NH2 > CN > RP-2 >
Recommended application:
Alkaloids, amino acids, preservatives, optical brighteners, barbiturates, polycyclic aromatic hydrocarbons
(PAH), drugs, peptides, flavonoids, phenols, indole derivatives, steroids
Ordering information
Plate size [cm]
Pack of [plates]
Glass plates
C18-50
C18-50 UV254
C18-100
C18-100 UV254
} 50% silanized
} 100% silanized
v/v
Methanol H2O
2:1
1:1
1:2
1:3
1:4
0:1
57
52
50
40
30
0
45
21
0
0
0
0
44
40
43
45
46
54
2:1
1:1
1:2
1:3
1:9
62
52
51
48
20
46
30
27
15
0
66
54
46
44
42
68
64
71
Acetonitrile H2O
Thickness of layer
Fluorescent
indicator
811054
811064
811052
811062
0.20mm
0.20mm
0.20mm
0.20mm
UV254
UV254
Trichloromethane
www.mn-net.com
60
Nano-SIL
Nano-SIL
Nano-SIL
Nano-SIL
10 x 10
25
c)
40
a)
b)
20
0
100
80
60
40
20
Reversed phase separation mode with eluents from anhydrous solvents to mixtures with high concentrations of water (see table and figure below)
0% H2O
methanol water;
acetonitrile water
219
Base material:
Nano silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60,
specific pore volume 0.75mL/g, particle size 210m;
for preparative plates (1mm thickness of layer) standard silica 60, particle size 517m
pH stability 210
Indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254nm); UV-absorbing substances appear as dark-blue to black spots on a light-blue background
Partial octadecyl (C18) modification, wettable with water, carbon content 14%
Order of polarity: silica > DIOL > NH2 > CN > RP-2 > C18-50 >
NP or RP separation with eluents from anhydrous solvents to mixtures with high concentrations of water
(see table and figure on previous page); relative polarity of the eluent determines the polarity of the layer
Recommended application: aminophenols, barbiturates, preservatives, nucleobases, polycyclic aromatic
hydrocarbons, steroids, tetracyclines, plasticizers (phthalates)
Ordering information
Plate size [cm]
4x8
5 x 10
5 x 20
Glass plates
Pack of [plates]
50
RP-18 W/UV254
Pack of [plates] (preparative TLC)
RP-18 W/UV254
811073
Pack of [plates]
RP-18 W/UV254
50
818145
10 x 10
25
811075
10 x 20
50
811072
20 x 20
Thickness of
layer
Fluorescent
indicator
25
811071
15
811074
0.25mm
UV254
1.00mm
UV254
25
818146
0.15mm
UV254
RP-2/UV254
50
818144
50
818152
25
818147
Base material:
Silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60,
specific pore volume 0.75mL/g, particle size 517m, pH stability 210
Indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254nm); UV-absorbing substances appear as dark-blue to black spots on a light-blue background
Normal phase or reversed phase separation modes with purely organic, organic - aqueous or purely aqueous eluents
Recommended application: active plant constituents, steroids
Ordering information
Glass plates
RP-2/UV254
220
10 x 20
50
20 x 20
25
Thickness of layer
Fluorescent
indicator
811081
811082
0.25mm
UV254
818171
0.15mm
UV254
info@mn-net.com
Base material:
Nano silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60,
specific pore volume 0.75mL/g, particle size 210m, pH stability 28
Indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254nm);
UV-absorbing substances appear as dark-blue to black spots on a light-blue background
* * Cortisone
Rf
1.0
0.8
0.6
0.4
0.2
0.0
*0 * 20
***
****
Partition
(reversed phase)
**
Adsorption
(normal phase)
40
100 80
60
% Petroleum ether
* *0
60
80 100 80
% Acetone
60
40
20
40
20
40
60
80 100
% Water
20
Nano-SIL CN/UV
400nL
ethanol water glacial acetic acid
(20:80:0.2) with 0.1mol/L tetraethyl
ammonium chloride
Migration distance: 7.3cm in 30min
Detection:
TLC scanner, UV 254nm
Peaks:
1. Propyl p-hydroxybenzoate
2. Ethyl p-hydroxybenzoate
3. Methyl p-hydroxybenzoate
4. Benzoic acid
5. Sorbic acid
2
start
Cholesterol
Androsterone
Separation of preservatives
Layer:
Sample volume:
Eluent:
73 mm
NP or RP separation modes depending on the polarity of the developing solvent (see figure below)
Ordering information
Plate size [cm]
Pack of [plates]
Glass plates
4x8
50
Nano-SIL CN/UV
Nano-SIL CN/UV
www.mn-net.com
818184
10 x 10
25
10 x 20
25
Thickness of
layer
Fluorescent
indicator
811115
811116
0.20mm
UV254
0.15mm
UV254
221
Base material:
Nano silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60,
specific pore volume 0.75mL/g, particle size 210m, pH stability 28
Indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254nm);
UV-absorbing substances appear as dark-blue to black spots on a light-blue background
NH2 > CN > RP-2 > C18-50 > RP-18 W > C18-100
Available with or without fluorescent indicator, as glass plates or ALUGRAM aluminium sheets
Layer can be wetted equally well with pure water as with organic solvents
Recommended application:
Vitamins, sugars, steroids, purine derivatives, xanthines, phenols, nucleotides and pesticides
Influence of eluent composition on the
separation of nucleotides
Rf
1.0
Adenosine
c-AMP
0.8
0.6
Nano-SIL NH2/UV
ethyl acetate pyridine water glacial
acetic acid (60:30:10:5, v/v/v/v)
Migration distance: 8cm in 45min, double development
Sample volume: 0.5L
Detection:
dry layer at 160C for 5min,
TLC scanner, UV 254nm
AMP
0.4
ADP
0.2
0.0
Separation of sugars
Layer:
Eluent:
*
0
100
** *
*
** ** *
20
80
40
60
60
40
80
20
ATP
5
4
100 % H2O
0
% MeOH
Layer:
Eluent:
Nano-SIL NH2/UV
MeOH H2O according to fig. + 0.18mol/L
NaCl
Migration distance: 7cm
c-AMP, AMP: adenosine monophosphate
ADP: adenosine diphosphate
ATP: adenosine triphosphate
50
mm
MN Appl. No. 401590
Ordering information
Plate size [cm]
Pack of [plates]
Glass plates
4x8
50
Nano-SIL NH2/UV
Nano-SIL NH2/UV
222
818182
10 x 10
25
10 x 20
25
Thickness of layer
Fluorescent
indicator
811111
811112
0.20mm
UV254
0.15mm
UV254
info@mn-net.com
Base material:
Nano silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60,
specific pore volume 0.75mL/g, particle size 210m, pH stability 28
Indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254nm);
UV-absorbing substances appear as dark-blue to black spots on a light-blue background
DIOL > NH2 > CN > RP-2 > C18-50 > RP-18 W > C18-100
Recommended application:
Steroids, pesticides and plant constituents; for critical separations an alternative to silica, since it is less
sensitive to the water content of the environment; leads to more reproducible results compared to silica
Separation of herbicides
Layer:
Sample volume:
Eluent:
Nano-SIL DIOL/UV
2L
petroleum ether
(4060C) acetone
(80:20, v/v)
Migration distance: 7cm
Detection:
TLC scanner, 238nm
Peaks:
(0.07% each in methanol)
1. Metoxuron
2. Monuron
3. Metobromuron
2
3
12.0
45.3
85.0 mm
Ordering information
Glass plates
Nano-SIL DIOL/UV
Thickness of layer
Fluorescent indicator
811120
0.20mm
UV254
Ordering information
Description
Glass plates:
3 plates 10 x 10cm (scored to 5 x 10cm) each of Nano-SIL C18-100/UV254, RP-18 W/UV254, RP-2/UV254, NanoSIL CN/UV, Nano-SIL NH2/UV, Nano-SIL DIOL/UV
ALUGRAM aluminium sheets:
5 sheets 4 x 8cm each of RP-18 W/UV254, RP-2/UV254, Nano-SIL CN/UV, Nano-SIL NH2/UV, Nano-SIL DIOL/UV
www.mn-net.com
REF
811001
818001
223
Aluminium oxide, specific surface (BET) ~ 200m2/g, mean pore size 60; inert organic binder
Indicator manganese-activated zinc silicate
Recommended application:
Terpenes, alkaloids, steroids, aliphatic and aromatic compounds
We recommend to activate aluminium oxide layers before use by heating 10 minutes at 120C.
Rf values
0.14
0.26
Compound
Bis[bis(4-phenyloxazolin)methane]fullerene 1
Bis[bis(4-phenyloxazolin)methane]fullerene 2
Ph
Ph
N
Ph
N
O
O
N
Ph
N
Layer:
Sample volume:
Eluent:
Alox-25 UV254
1000nL
toluene cyclohexane
(2:1, v/v)
Migration distance: 10.8cm in 15min
Detection:
TLC scanner,
UV 254nm
Peaks:
1. Indophenol
1
2. Sudan red G
3. Sudan blue II
4. Butter yellow
O
Ph
Ph
Ph
Ph
0.0
25.0
50.0
75.0
125.0 mm
MN Appl. No. 403010
Ordering information
Plate size [cm]
5 x 20
20 x 20
Pack of [plates]
Alox-25 UV254
Pack of [plates] (preparative TLC)
Alox-100 UV254
100
807021
25
807023
15
807033
Pack of [plates]
Alox N/UV254
50
802022
50
818024
Glass plates
4x8
Thickness of layer
Fluorescent indicator
0.25mm
UV254
1.00mm
UV254
25
802023
0.20mm
UV254
25
818023
0.20mm
UV254
50
802021
Pack of [plates]
Alox N/UV254
224
info@mn-net.com
Fiber length (95%) 220m, average degree of polymerization 400500, specific surface acc. to Blaine
15000cm2/g
20ppm Fe, 6ppm Cu, 7ppm P; CH2Cl2 extract 0.25%; residue on ignition at 850C 1500ppm
Recommended application:
Partition chromatography of polar substances such as amino acids, carboxylic acids or carbohydrates
Ordering information
Plate size [cm]
4x8
5 x 20
20 x 20
Pack of [plates]
300-10
300-10 UV254
300-25
300-25 UV254
Pack of [plates] (preparative TLC)
CEL 300-50
CEL 300-50 UV254
Pack of [plates]
CEL 300
CEL 300 UV254
50
818155
0.10mm
0.10mm
0.25mm
0.25mm
UV254
UV254
0.50mm
0.50mm
UV254
25
801013
801023
0.10mm
0.10mm
UV254
25
818153
818156
0.10mm
0.10mm
UV254
25
808013
808023
808033
808043
20
808053
808063
CEL
CEL
CEL
CEL
Pack of [plates]
CEL 300
CEL 300 UV254
Fluorescent
indicator
50
801022
50
818157
Prepared by hydrolysis of high purity cellulose with HCl; average degree of polymerization 40200
Recommended application:
Carboxylic acids, lower alcohols, urea and purine derivatives
Ordering information
Glass plates
CEL 400-10
CEL 400
CEL 400 UV254
www.mn-net.com
10 x 20
50
20 x 20
25
Thickness of layer
Fluorescent
indicator
808072
808073
0.10mm
801113
801123
0.10mm
0.10mm
UV254
Glass plates
Thickness of
layer
225
Recommended application: analysis of nucleic acids, and of mutagenic substances with the
ling procedure (see application 402260 at www.mn-net.com/apps)
32
P postlabel-
Ordering information
Plate size [cm]
Pack of [plates]
20 x 20
25
Thickness of layer
Fluorescent
indicator
801053
801063
0.10mm
0.10mm
UV254
Fibrous cellulose with 10% content of acetylated cellulose for reversed phase chromatography
Ordering information
Plate size [cm]
Pack of [plates]
Acetyl content
10%
Polyamide-6
20 x 20
25
Thickness of layer
Fluorescent indicator
801033
0.10mm
Separation mechanism based on hydrogen bonds to amide groups of the polymer matrix as well as on
ionic, dipole and electron donor-acceptor interactions
Recommended application:
Natural compounds, phenols, carboxylic acids, aromatic nitro compounds and especially amino acids
Ordering information
Plate size [cm]
Pack of [plates]
5 x 20
50
20 x 20
25
Thickness of layer
Fluorescent indicator
803012
803022
803013
803023
0.10mm
0.10mm
UV254
CHIRALPLATE
Reversed phase nano silica impregnated with Cu2+ ions and a chiral selector (a proline derivative,
DP3143726 and EP 0 143 147)
Separation based on ligand exchange, i.e. formation of ternary mixed-ligand complexes with the Cu(II)
ions; differences in the stability of the diastereomeric complexes cause chromatographic separation
A review on the application of CHIRALPLATE has been given by K. Gnther [J. Chromatogr. 448 (1988) 1130].
226
info@mn-net.com
a)
b)
c)
d)
5 x 20
10 x 10
10 x 20
20 x 20
25
811059
4
811056
25
811055
25
811058
Glass plates
Pack of [plates]
CHIRALPLATE
Pack of [plates]
CHIRALPLATE
50
811057
SIL N-HR
Thickness of
layer
Fluorescent
indicator
0.25mm
UV254
0.25mm
UV254
High purity silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60,
specific pore volume 0.75mL/g, particle size 517m
Ordering information
Plate size [cm]
Pack of [plates]
5 x 20
50
20 x 20
25
Thickness of layer
Fluorescent indicator
804022
804023
0.20mm
UV254
SIL G-25 HR
Ordering information
High purity silica 60 with gypsum and a very small quantity of a polymeric organic binder
softer than the standard silica layer, i.e. spots can be scratched and the layer absorbs faster
Recommended application: aflatoxins
Ordering information
Plate size [cm]
Pack of [plates]
Glass plates
SIL G-25 HR
SIL G-25 HR/UV254
www.mn-net.com
20 x 20
25
Thickness of layer
Fluorescent indicator
809033
809043
0.25mm
0.25mm
UV254
227
Ordering information
Glass plates
Thickness of layer
Fluorescent indicator
810063
0.25mm
Nano-SIL PAH
Base material: nano silica 60, specific surface (BET) ~ 500m2/g, mean pore size 60,
specific pore volume 0.75mL/g, particle size 210m; impregnated with caffeine, an electron acceptor for
PAH analysis based on charge-transfer complexes
Recommended application: 6 PAHs according to German drinking water specifications (TVO) in accordance with German standard DIN 38407 part 7 (see application 402400 at www.mn-net.com/apps)
Ordering information
Glass plates
Thickness of layer
Fluorescent indicator
811051
0.20mm
Nano-SIL PAH
IONEX
IONEX-25 SA-Na: mixture of silica and a strongly acidic cation exchanger coated to polyester sheets
IONEX-25 SB-AC: mixture of silica and a strongly basic anion exchanger coated to polyester sheets
Both layers contain an inert organic binder
Recommended application:
Amino acids, e.g., in protein and peptide hydrolyzates, in seeds and fodder, in biological fluids; for racemate separation in peptide syntheses, for the separation of nucleic acid hydrolyzates, aminosugars, amino
acids, antibiotics, inorganic phosphates, cations and other compounds with ionic groups
Ordering information
POLYGRAM polyester sheets
IONEX-25 SA-Na
IONEX-25 SB-AC
228
20 x 20
25
Thickness of layer
Fluorescent
indicator
806013
806023
0.20mm
0.20mm
info@mn-net.com
Ordering information
Plate size [cm]
Pack of [plates]
Alox/CEL-AC-Mix-25
SILCEL-Mix-25 UV254
Thickness of layer
Fluorescent indicator
810053
810043
0.25mm
0.25mm
UV254
Chromatography papers
Chromatography papers possess a preferred direction of the fibers with higher absorption properties (with
our sheets 58 x 60cm, the longer edge). We recommend to use them in the direction of higher absorption.
Ordering information
Code
MN
MN
MN
MN
MN
MN
MN
MN
214
218
260
261
827
866
866
214 ff
Weight
[g/m2]
140
180
90
90
270
650
650
140
Thickness
[mm]
0.28
0.36
0.20
0.18
0.70
1.70
1.70
0.28
Description
smooth
smooth
smooth
smooth
soft carton
soft carton
soft carton
MN 214 defatted *
Flow rate
90100mm/30min
90100mm/30min
120130mm/30min
90100mm/30min
130140mm/10min
100120mm/10min
100120mm/10min
90100mm/30min
Size [cm]
58
58
58
58
58
38
80
56
x
x
x
x
x
x
x
x
60
60
60
60
60
38
80
58
Pack of
REF
100sheets
100sheets
100sheets
100sheets
100sheets
100sheets
100sheets
100sheets
817001
817002
817003
817004
817005
817006
817007
817008
Glass plates
20 x 20
25
For further papers, filters and membranes, feel free to ask for our catalog Filtration
www.mn-net.com
229
Beginners set
features separations with simple developing solvents; samples are colored thus eliminating the need for
visualization. All equipment needed is contained in the set.
Advanced sets
require some experience and skill from the user: some of the samples have to be pretreated before separation, and for identification of substances spray reagents have to be used
TLC micro-set M
Ordering information
Designation
Test dye mixture 1*, solution of 4 lipophilic dyes in toluene (components see above)
Test dye mixture 2*, solution of 7 anthraquinone dyes in toluene cyclohexane (2:2, v/v)
(components see above)
Test dye mixture 3, aqueous solution of 7 food dyes (components see above)
Collection of 4 individual components of test dye mixture 1*
Collection of 7 individual components of test dye mixture 2*
Collection of 7 individual components of test dye mixture 3
Sodium citrate, 2.5g in 100mL bottles to fill up with distilled water
Pack of
REF
1 kit
814000
8mL
8mL
814001
814002
8mL
4 x 8mL
7 x 8mL
7 x 8mL
2.5g
814003
814011
814012
814013
814029
1 kit
814100
* These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.
230
info@mn-net.com
TLC micro-set F3
www.mn-net.com
TLC micro-set F2
231
Pack of
REF
1 kit
814200
8mL
4 x 8mL
8mL
2 x 8mL
814201
814202
814204
814205
1 kit
814300
8mL
814301
1 kit
814400
8mL
8mL
8mL
814405
814406
814407
4 x 50
4 x 50
4 x 50
1 set
814025
814026
814027
814028
TLC
TLC
TLC
TLC
polyester
polyester
polyester
polyester
sheets
sheets
sheets
sheets
POLYGRAM
POLYGRAM
POLYGRAM
POLYGRAM
* These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.
Ordering information
Designation
Pack of
REF
1 set
814500
232
info@mn-net.com
REF
814019
814018
814021
814101
814022
814102
814104
814023
814024
818666
814103
531011
814030
Designation
Simultaneous developing chamber for TLC, 20 x 20cm, for up to 5 plates
Simultaneous developing chamber for TLC, 10 x 10cm, for up to 2 plates
Developing chambers for TLC micro-sets
Glass laboratory sprayer with rubber bulb
Glass capillaries 1L
Rubber caps for capillaries
Plastic syringe, 1mL content with graduation
Spotting guides
Measuring cylinders, glass, 10mL content
MN ALUGRAM scissors, ground blade, black handle
Filter paper MN 713, 15 x 21cm
Folded filters MN 615 1/4, 11cm diameter
Chromatography paper MN 260, 7.5 x 17cm (for chamber saturation)
814019
818666
814018
Visualization reagents
Small selection of frequently used spray reagents for postchromatographic detection reactions in TLC
suited for spraying or dipping TLC plates
A detailed description of many more detection procedures for TLC is available on request.
Ordering information
Spray reagent
Aniline phthalate
Solvent
2-propanol ethanol
(1:1)
Bromocresol green
2-propanol
Reagent for caffeine
water acetone
2,7-Dichlorofluorescein
2-propanol
4-(Dimethylamino)-benzaldehyde 2-propanol
according to Dragendorff-Munier water
Iron(III) chloride
water
Potassium hexacyanoferrate(III)
water
Molybdatophosphoric acid
ethanol
Ninhydrin
ethanol
Rhodamin B
ethanol
Rubeanic acid
ethanol
Detection of
reducing sugars, oxohalic acids
organic acids
caffeine
lipids (saturated, unsaturated)
terpenes, sugars, steroids
alkaloids and other nitrogen compounds
Pack of
REF
100mL 814919
100mL
100mL
100mL
100mL
100mL
100mL
acetylsalicylic acid, paracetamol
100mL
lipids, sterols, steroids, reducing compounds 100mL
amino acids, amines and amino sugars
100mL
lipids
100mL
heavy metal cations
100mL
814920
814401
814921
814922
814402
814403
814404
814302
814203
814923
814206
These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.
www.mn-net.com
233
Pore size 60, pore volume 0.75mL/g, specific surface (BET) ~ 500m /g, pH 7 for a 10% aqueous suspension
2
Silica G: standard grade, particle size 220m, Fe < 0.02%, Cl < 0.02%, 13% gypsum as binder,
supplied with or without fluorescence indicator UV254
Silica N: standard grade, particle size 220m, Fe < 0.02%, Cl < 0.02%, no binder,
supplied with or without fluorescence indicator UV254
Silica G-HR: high purity grade, particle size 320m, Fe < 0.002%, Cl < 0.008%, gypsum as binder,
supplied without fluorescence indicator
Silica P: preparative grade, particle size 550m, Fe < 0.02%, Cl < 0.02%, organic binder,
supplied with fluorescence indicator UV254
Silica P with gypsum: preparative grade, particle size 550m, Fe < 0.02%, Cl < 0.02%, gypsum as
binder, supplied with fluorescence indicator UV254
Ordering information
Designation
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Fluorescent indicator
G
G/UV254
N
N/UV254
G-HR
P/UV254
P/UV254 with gypsum
UV254
UV254
UV254
UV254
1kg
5kg
816310.1
816320.1
816330.1
816340.1
816410.1
816380.1
816400.1
Polyamide
816310.5
816320.5
816330.5
816340.5
816410.5
816380.5
816400.5
Ordering information
Designation
Polyamide TLC 6
Polyamide TLC 6 UV254
Fluorescent indicator
UV254
Cellulose MN 301
1kg
816610.1
816620.1
Native fibrous cellulose, standard grade; fiber length (95%) 220m, average degree of polymerization
400500, specific surface acc. to Blaine 15000cm2/g
20ppm Fe, 6ppm Cu, 7ppm P, CH2Cl2 extract 0.25%, residue on ignition at 850C 1500ppm
Ordering information
Designation
Cellulose MN 301
Fluorescent indicator
1kg
816250.1
5kg
816250.5
Fluorescent indicators
UV254: manganese activated zinc silicate with absorption maximum at 254nm; green fluorescence; relatively susceptible towards acids; thus its fluorescence can be completely quenched by acidic solvents
UV366: inorganic fluorescent pigment with absorption maximum at 366nm; blue fluorescence
Ordering information
Fluorescent indicator UV254
Fluorescent indicator UV366
234
Composition
green
blue
Pack of 100g
816710.01
816720.01
info@mn-net.com
GC Contents
Basic principles of GC
236
238
264
Fast GC
Enantiomer separation
Analysis of biodiesel
High temperature GC
Untreated capillaries, retention gaps and deactivated capillaries
266
267
268
272
274
276
280
Reagents for GC
Derivatization reagents
282
Methylation reagents
284
Acylation reagents
Silylation reagents
283
285
289
291
Accessories for GC
Ferrules for GC
293
Septa
296
Valco fused silica adapters and other connectors for capillary columns
Tools and general accessories for GC
www.mn-net.com
294
297
Gas Chromatography
Amine separation
242
235
Chromatographic separation is achieved through continuous distribution of each sample component between the mobile and the stationary phase:
In GC, the mobile phase is always a gas, either He, N2,
Ar, or H2.
1
3
5
6
7
The chromatogram
2. Sample injector:
During direct injection, the sample is applied
to the column without touching any other parts
made from glass or metal (on-column injection).
During indirect injection, the sample is brought
into an evaporator and is then transferred onto
the column either completely, or partially (split
technique).
Both techniques allow working at low temperatures, high temperatures and the use of temperature programming.
236
tR 1
t0: dead time = residence time of a solute in the mobile phase (time required by a component to migrate through the chromatographic system without
any interaction with the stationary phase)
t Ri t 0
t0
info@mn-net.com
k1
The relative retention does not provide any information on the quality of a separation. For equal
values of two very broad peaks may overlap (as
shown in a), or may be completely resolved (as in
b), if they are accordingly narrow.
resolution: a measure for the quality of a separation, taking w1/2 into account according to
t R2 t R1
(w1/2)2 + (w1/2)1
( w )
Nth = 5.54
t Ri
1/2
h = HETP =
Nth
The Golay equation shows how the plate height h depends on the flow velocity u:
HETP
B
HETP = ---- + C u
u
hmin
B
u
uopt.
B molecular axial diffusion; B is a function of the diffusion coefficient of the component in the respective carrier gas
www.mn-net.com
A. Stationary phase
Different chemical structures of stationary phases
are responsible for the type of interaction (selectivity) between the phase and the analytes. The
stationary phase also limits the temperature range
for chromatography. For a detailed summary of MN
phases for GC please see the following chapter.
B. Film thickness
ranges from 0.1 to 5.0m. The standard film
thickness is 0.25m. Thin films (0.10.2m) are
very well suited for high-boiling, temperaturesensitive or almost contemporaneously eluting
substances. Increasing the film thickness will
increase the capacity, the retention for low-boiling
substances and the inertness of the column. This is
especially helpful for samples with a broad range of
concentrations, or the separation of volatile polar
substances.
Thick films, however, always mean more stationary phase in the column, hence increased column
bleeding. Therefore, maximum operating temperatures for thick-film columns are reduced. In addition, thick-film columns may have a lesser separating capacity.
C. Column length
The separating efficiency (better the number of
plates N) of a column is directly proportional to its
length. Most routine separations are carried out on
25 or 30m columns, while more complex samples
may require 50 or 60m. 10m columns are common for Fast GC (see page 267).
t [min]
R=
k2
R:
237
Polar phases offer additional interactions that may improve a separation. When the polarity is increased, e.g.,
by introducing phenyl and/or cyanopropyl groups, differences in dipole moment and charge transfer effects,
e.g., in 550% diphenylpolysiloxane phases, gain more
and more influence on the separation. Typical analytes
are hydrocarbons containing oxygen, sulfur, nitrogen,
phosphorus or halogens, as well as unsaturated, polarizable molecules and aromatics.
Phase
The selectivity of a column has to be optimized for either the critical pair of components, or the main constituent. Always select the least polar column your
separation works on. About 70% of all separations can
be accomplished on non- to midpolar columns. These
columns generally show a high temperature stability.
For GC columns for special separations, please go to
page 266.
Max. temperature 1
USP
Similar phases
OPTIMA 1
100% dimethylpolysiloxane
340/360 C
G1
G2
G38
OPTIMA 1 MS
OPTIMA
1 MS Accent
100% dimethylpolysiloxane
340/360 C
G1
G2
G38
340/360 C
G27
G36
340/360 C
G27
G36
340/360 C
G27
G36
OPTIMA 5
OPTIMA 5 MS
OPTIMA
5 MS Accent
5% phenyl
95% methylpolysiloxane
5% diphenyl
95% dimethylpolysiloxane
silarylene phase with selectivity
similar to 5% diphenyl
95% dimethylpolysiloxane
OPTIMA XLB
340/360 C
OPTIMA -3
340/360 C
OPTIMA -6
OPTIMA 1301
OPTIMA 624
OPTIMA 624 LB
OPTIMA 1701
238
Composition
Page
242
243
244
245
246
247
248
G49
no similar phases
249
340/360 C
no similar phases
250
6% cyanopropylphenyl
94% dimethylpolysiloxane
300/320 C
G43
251
6% cyanopropylphenyl
94% dimethylpolysiloxane
280/300 C
G43
280/300 C
G43
252
14% cyanopropylphenyl
86% dimethylpolysiloxane
300/320 C
G46
253
info@mn-net.com
Composition
Max. temperature 1
USP
Similar phases
G28
G32
G42
OPTIMA 17
phenylmethylpolysiloxane, 50%
phenyl
320/340 C
G3
OPTIMA 17 MS
340/360 C
G3
OPTIMA 210
trifluoropropylmethylpolysiloxane
(50% trifluoropropyl)
260/280 C
G6
OPTIMA 225
260/280 C
G7
G19
OPTIMA 240
260/280 C
360/370 C
Page
254
255
256
257
258
no similar phases
259
PERMABOND CW 20 M (page265),
DB-Wax, Supelcowax, HPWax,
HPINNOWAX, RtxWax, CP-Wax
52 CB, Stabilwax, 007-CW, BP20,
ATWax, ZB-Wax
DB-Wax, Supelcowax, HPWax,
HPINNOWAX, RtxWax, CP-Wax 52
CB, Stabilwax, 007-CW, BP20, ATWax, ZB-Wax
OPTIMA WAX
240/250 C
G16
OPTIMA WAXplus
260/270 C
G16
OPTIMA FFAP
240/250 C
G35
G25
262
250/260 C
G35
G25
263
OPTIMA 35 MS
260
261
First temperature for isothermal operation, second value for short isotherms in a temperature program
Please note that for columns with 0.53mm ID and for columns with thicker films temperature limits are generally lower.
For details refer to the description of individual phases.
2
Phases which provide a similar selectivity based on chemical and physical properties
3
See description on page 241
1
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.
Columns have fused ends or are sealed with septa, to protect them from atmospheric oxygen. A standard test mixture is included with every column.
On request, all columns can be supplied on a 5 inch (13cm) cage for the Agilent GC 6850. To order, please add an E at the
end of the REF number (e.g., 726470.30E)
To prolong column life, even at highly contaminated or matrix-containing samples, MN offers the option to add an integrated
guard column. All capillary columns are available with a 10m guard column with respective deactivation. To order, please add
V1 at the end of the REF number. Guard column combinations with other lengths, IDs or different deactivation are available on
request.
www.mn-net.com
239
2
36 7
12
3 6
7
OPTIMA FFAP
OPTIMA FFAPplus
8 9 10
OPTIMA WAX
OPTIMA WAXplus
8 9 10
1 2
3
6 7 4
8 9 10
OPTIMA 240
5
2
8 9 10
OPTIMA 225
5
2
1 3
1
3
All columns:
Sample:
Injection:
Carrier gas:
Temperature:
Detector:
8 9 10
OPTIMA 210
46
546
2
45 6
2
4 5
6
8 9 10
8 9 10
546
240
6 74
8 9 10
8 9 10
8 9 10
8 9 10
OPTIMA 17
OPTIMA 17 MS
OPTIMA 1701
increasing polarity
OPTIMA 35 MS
OPTIMA 1301
OPTIMA 5
OPTIMA 5 MS
OPTIMA 5 MS Accent
OPTIMA 5 HT
OPTIMA 1
OPTIMA 1 MS
OPTIMA 1 MS Accent
Peaks:
1. Undecane
2. Dodecane
3. Octanol
4. Dimethylaniline
5. Decylamine
6. Methyl decanoate
7. Methyl undecanoate
8. Henicosane
9. Docosane
10. Tricosane
info@mn-net.com
OPTIMA
1 2
3
5 46 7
1 2
3 54 6
8 9 10
OPTIMA 17
8 9 10
1 2
5
3
4 6 7
8 9 10
1 2
3 45
6 7
8 9 10
OPTIMA 1701
OPTIMA -6
240
M
A
P TI M A
OPTI
1701
0
21
6
-
3
-
OPTIM
A
permanent
+ induced
dipole: variable
interactions =
autoselectivity
1 2
3 45 6 7
OPTIMA -3
8 9 10
OPTIMA 1301
241
100% dimethylpolysiloxane
Nonpolar phase
CH3
Si
CH3
USP G1/G2/G38
Thick film columns 3m film are especially recommended for solvent analysis.
Ordering information
Length
10m
12m
15m
726024.10
film
film
film
film
0.10m
0.25m
0.50m
1.00m
film
film
film
film
726038.10
726050.10
726081.10
0.10m
0.25m
0.35m
0.50m
1.00m
3.00m
5.00m
film
film
film
film
film
film
film
726301.10
726302.10
726302.15
726304.10
726323.10
726323.15
0.50m
1.00m
2.00m
5.00m
film
film
film
film
726834.12
726837.12
726038.15
726050.15
726931.10
726529.10
726521.10
726926.10
30m
726832.25
726834.25
726837.25
0.10m
0.20m
0.35m
0.50m
25m
50m
60m
726024.20
726025.20
20m
726519.15
726529.15
726834.50
726837.50
726839.50
726038.25
726050.25
726081.25
726802.25
726038.30
726050.30
726081.30
726802.30
726050.50
726081.50
726802.50
726301.25
726302.25
726821.25
726304.25
726323.25
726805.25
726931.25
726301.30
726302.30
726821.30
726304.30
726323.30
726805.30
726931.30
726301.50
726302.50
726821.50
726304.50
726323.50
726805.50
726931.50
726519.25
726529.25
726521.25
726926.25
726519.30
726529.30
726521.30
726926.30
726521.50
726926.50
726038.60
726050.60
726081.60
726802.60
726301.60
726302.60
726821.60
726304.60
726323.60
726805.60
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.
Columns have fused ends or are sealed with septa, to protect them from atmospheric oxygen. A standard test mixture is included with every column.
On request, all columns can be supplied on a 5 inch (13cm) cage for the Agilent GC 6850. To order, please add an E at the
end of the REF number (e.g., 726470.30E)
To prolong column life, even at highly contaminated or matrix-containing samples, MN offers the option to add an integrated
guard column. All capillary columns are available with a 10m guard column with respective deactivation. To order, please add
V1 at the end of the REF number. Guard column combinations with other lengths, IDs or different deactivation are available on
request.
242
info@mn-net.com
11. 1-Methoxy-2-propanol
12. Isooctane
13. Ethyl glycol
14. Isoheptane
15. Methyl isobutyl ketone
16. 1-Ethoxy-2-propanol
17. Toluene
18. Isobutyl acetate
19. Butyl acetate
20. 4-Hydroxy-4-methyl-2-pentanone
21. 1-Methoxy-2-propyl acetate
22. Xylene
23. Cyclohexanone
24. Ethyl glycol acetate
25. Butyl glycol
26. Heptanol
0
27. Ethyl diglycol
28. Butyl diglycol
29. Butyl glycol acetate
30. Butyl diglycol acetate
11
3
5
12
19
15
13
14
16
28
29
25
10
20
30
min
100% dimethylpolysiloxane
Max. temperature for isothermal operation
340C, max. temperature for short isotherms
in a temperature program 360C
CH3
27
24
17
20
26
21
22
OPTIMA 1 MS
30
23
USP G1/G2/G38
Column:
OPTIMA 1, 1.0m film, 60m x 0.32mm ID
Sample:
solvent mixture, courtesy of J. Lutz, Alcan Rorschach, Switzerland
Injection:
0.4L, split 1: 60
Carrier gas:
H2, 120 kPa
Temperature:
50C (9min) 90C, 4C/min 280C (2min), 14C/min
Detector:
FID 300C
Peaks:
1. Methanol
24
2. Ethanol
6
1
3. Acetone
4. 2-Propanol
5. Methyl acetate
9
6. n-Propanol
10
7. Methyl ethyl ketone
8. Ethyl acetate
9. Isobutanol
18
7
10. n-Butanol
Ordering information
0.2mm ID (0.4mm OD)
Length
0.20m film
0.35m film
12m
15m
25m
30m
726201.25
726203.12
726205.15
50m
726201.50
726205.30
0.25m film
726202.30
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
www.mn-net.com
60m
726205.60
726202.60
243
CH3
Si
CH3
Column:
Sample:
Injection:
Carrier gas:
Temperature:
100% dimethylpolysiloxane
Lowest column bleed, nonpolar phase, ideal for ion trap and
quadrupole MS detectors
perfect inertness for basic compounds
solvent rinsing for removal of impurities applicable
Detector:
Peaks:
1. Dichlorvos, 2. Hexamethylphosphoramide, 3.Mevinphos,
4.Trichlorfon, 5. TEPP, 6. Thionazin, 7.Demeton-O, 8.Ethoprop
9.Tributyl phosphate(IS), 10. Dicrotophos, 11. Monocrotophos,
12.Naled, 13.Sulfotepp, 14. Phorate, 15.Dimethoate, 16.DemetonS,
17. Dioxathion, 18. Terbufos, 19.Fonophos, 20.Phosphamidon isomer,
21.Diazinon, 22. Disulfoton, 23.Phosphamidon, 24.Dichlorofenthion,
25.Parathionmethyl, 26.Chlorpyrifos methyl, 27.Ronnel,
28.Fenitrothion, 29.Malathion, 30. Fenthion, 31.Aspon,
32.Parathionethyl, 33.Chlorpyrifos, 34.Trichloronate,
35.Chlorfenvinphos, 36.Merphos, 37. Crotoxyphos, 38.Stirofos,
39.Tokuthion, 40.Merphos oxidation product, 41.Fensulfothion,
42.Famphur, 43.Ethion, 44.Bolstar, 45.Carbophenothion, 46.Triphenyl
phosphate(IS), 47.Phosmet, 48. EPN, 49. Azinphos-methyl,
50.Leptophos, 51.Tri-o-cresyl phosphate, 52.Azinphos-ethyl,
53.Coumaphos
13
6 9
10
11
12 8
1
4
51
31
32
33
43
44
24
25
42
18
19
45
27
48
36
30
46 47
26 28
37
15
41
29
14
49 50
34
21
38
5253
22
35 39
40
23
16
17
7
20
12
16 min
Ordering information
Length
15m
0.25m film
0.50m film
30m
725801.25
0.20m film
25m
725805.15
50m
60m
725801.50
725805.30
725806.30
725805.60
725806.60
725802.30
725807.30
725802.60
725807.60
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.
Columns have fused ends or are sealed with septa, to protect them from atmospheric oxygen. A standard test mixture is included with every column.
244
info@mn-net.com
Nonpolar phase
CH3
Si
CH3
O
Si
CH3
Length
10m
15m
25m
30m
50m
Ordering information
60m
726846.10
film
film
film
film
0.10m
0.25m
0.35m
0.50m
1.00m
film
film
film
film
film
0.10m
0.25m
0.35m
0.50m
1.00m
3.00m
5.00m
film
film
film
film
film
film
film
726313.10
0.50m
1.00m
2.00m
5.00m
film
film
film
film
726523.10
726541.10
726525.10
726916.10
726854.25
726857.25
726860.25
726863.25
726056.15
726313.15
726314.15
726325.15
726934.15
726541.15
726857.50
726860.50
726863.50
726911.25
726056.25
726623.25
726099.25
726807.25
726911.30
726056.30
726623.30
726099.30
726807.30
726911.50
726056.50
726623.50
726099.50
726807.50
726911.60
726056.60
726623.60
726099.60
726807.60
726313.25
726314.25
726628.25
726316.25
726325.25
726809.25
726934.25
726313.30
726314.30
726628.30
726316.30
726325.30
726809.30
726934.30
726313.50
726314.50
726628.50
726316.50
726325.50
726809.50
726934.50
726313.60
726314.60
726628.60
726316.60
726325.60
726809.60
726523.25
726541.25
726525.25
726916.25
726523.30
726541.30
726525.30
726916.30
726525.50
726916.50
726525.60
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
On request, all columns can be supplied on a 5 inch (13cm) cage for the Agilent GC 6850. To order, please add an E at the
end of the REF number (e.g., 726470.30E)
To prolong column life, even at highly contaminated or matrix-containing samples, MN offers the option to add an integrated
guard column. All capillary columns are available with a 10m guard column with respective deactivation. To order, please add
V1 at the end of the REF number. Guard column combinations with other lengths, IDs or different deactivation are available on
request.
www.mn-net.com
245
CH3
O
Si
Si
CH3
USP G27/G36
Column:
OPTIMA 5 MS, 30m x 0.25mm ID, 0.25m film
Sample:
5ppm of each compound except N-i-propylaniline (9.4ppm)
Method:
SPME
Temperature:
40C (2min) 240C, 6C/min 320C, 20C/min
Detector:
MSD
Peaks:
1. Toluene-D8
2. Phenol
3. 2-Methylphenol (o-Cresol)
4. Nitrobenzene-D5
5. N-i-Propylaniline
6. 2,4-Dichlorophenol
7. 4-Chlorophenol
8. 4-Bromo-2-chlorophenol
9. 3-Bromophenol
10. 4-Chloro-3-methylphenol
11. 2,4-Dibromophenol
12. 2-Hydroxybiphenyl
13. 2-Cyclohexylphenol
14. Hexafluorobisphenol A
12
14
10
5
7
1
34
13
2
6
10
11
20
30
min
Ordering information
Length
12m
15m
726210.12
726215.12
25m
726210.25
726215.25
726220.15
50m
726212.25
60m
726210.50
726215.50
726220.30
726225.30
726226.30
30m
726211.30
726213.30
726220.60
726225.60
7262267.60
726212.50
726212.60
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
246
info@mn-net.com
silarylene phase
Si
O
m
CH3
CH3
Si
Si
CH3
CH3
CH3
O
Si
n
CH3
USP G27/G36
Recommended application: all-round phase for environmental analyses, trace analyses, EPA methods,
pesticides, PCB, food and drug analyses
Similar phases:
DB-5MS, HP-5MS, Ultra-2, Equity-5, CP-Sil 8 CB low
bleed/MS, Rxi-5MS, Rtx-5SIL-MS, Rtx5MS, 0075MS, BPX5, MDN-5S, AT-5MS, VF-5MS
FID signal in mV
107.8
90.2
Standard 5 MS phase
107.6
90
107.4
89.8
107.2
89.6
10
20
30 s
OPTIMA 5 MS Accent
10
20
30 s
Ordering information
Length
12m
15m
25m
725810.25
725815.12
725820.15
30m
725812.25
50m
60m
725810.50
725815.50
725820.30
725825.30
725826.30
725820.60
725825.60
725826.60
725811.30
725813.30
725811.60
725812.60
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.
Columns have fused ends or are sealed with septa, to protect them from atmospheric oxygen. A standard test mixture is included with every column.
www.mn-net.com
247
silarylene phase
Si
O
m
CH3
CH3
Si
Si
CH3
CH3
CH3
O
Si
CH3
Column:
Injection:
Carrier gas:
Temperature:
Detector:
8: PCB-31
9: PCB-28
5
82% separation
in less than 10min!
6
25
26
benzo[b]fluoranthene /
benzo[k]fluoranthene
75% separation in 17min!
11 13
15
21
8/9
10
12 14 1617 18 19 20
10
12
14
22
25 26 27
23
24
16
18
29
30
28
20 min
MN Appl. No. 212920
Peaks:
1. Naphthalene
2. 2-Methylnaphthalene
3. Acenaphthylene
4. Acenaphthene
5. Fluorene
6. Phenanthrene
7. Anthracene
8. PCB-31
9. PCB-28
10. PCB-52
11. Fluoranthene
12. PCB-101
13. Pyrene
14. PCB-77
15. 2-Methylfluoranthene
16. PCB-118
17. PCB-153
18. PCB-138
19. PCB-126
20. PCB-180
21. Benz[a]anthracene
22. Chrysene
23. PCB-169
24. PCB-194
25. Benzo[b]fluoranthene
26. Benzo[k]fluoranthene
27. Benzo[a]pyrene
28. Dibenz[ah]anthracene
29. Indeno[1,2,3-cd]pyrene
30. Benzo[ghi]perylene
Ordering information
Length
30m
60m
725850.30
725850.60
On request, all columns can be supplied on a 5 inch (13cm) cage for the Agilent GC 6850. To order, please add an E at the
end of the REF number (e.g., 726470.30E)
248
info@mn-net.com
USP G49
9
13
12
10
11
15
14
18
17
16
20
21
22
19
20
min
MN Appl. No. 250060
Ordering information
Length
10m
20m
726410.10
726410.20
25m
30m
726400.25
50m
60m
726400.50
0.25m film
0.50m film
726420.30
726421.30
726420.60
0.25m film
0.35m film
1.00m film
726440.30
726441.30
726442.30
726440.60
726441.60
726442.60
1.00m film
726443.30
Column:
OPTIMA -3, 0.25m film, 60m x 0.25mm ID
Injection:
1.0L, split 1: 80
5
Carrier gas:
He, 1.3bar
2
3
Temperature:
60C (3min) 320C, 6C/min
Detector:
MSD HP 5971
Peaks:
4
1. Phenol
2. 2-Chlorophenol
3. 2-Methylphenol
1
4. 4-Methylphenol
5. 3-Methylphenol
6. 2,4-Dimethylphenol
7. 2-Nitrophenol
8. 2,4-Dichlorophenol
9. 2,6-Dichlorophenol
10. 4-Chloro-3-methylphenol
11. 2,3,5-Trichlorophenol
12. 2,4,6-Trichlorophenol
13. 2,4,5-Trichlorophenol
14. 2,3,4-Trichlorophenol
15. 2,3,6-Trichlorophenol
16. 2,3,5,6-Tetrachlorophenol
17. 2,3,4,5-Tetrachlorophenol
18. 2,3,4,6-Tetrachlorophenol
19. 2,4-Dinitrophenol
20. 3,4,5-Trichlorophenol
10
21. 2-Methyl-4,6-dinitrophenol
22. 2-Isopropyl-4,6-dinitrophenol
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
www.mn-net.com
249
Column:
OPTIMA -6, 0.2m film, 50m x 0.2mm ID
Sample:
EPA 8140 OP pesticide calibration mix (Restek), 200g/mL each in hexane acetone (95:5)
Injection:
1L, split 1: 30
8
12 13
Carrier gas:
2.0bar He
10
Temperature:
150C 300C (10min), 2.5C/min
14
Detector:
MSD HP 5971
19
16
9
20
Peaks:
1
6
1. Dichlorvos
17
4
2. Mevinphos
3. Demeton-S
4. Ethoprop
5. Naled
6. Phorate
2
7. Demeton-O
8. Diazinon
9. Disulfoton
7
11
10. Ronnel
11. Parathion-methyl
12. Chlorpyrifos
5
15
13. Trichloronate
14. Fenthion
18
15. Merphos
16. Stirofos
17. Tokuthion
18. Merphos oxidation product
19. Fensulfothion
3
20. Bolstar
21. Azinphos-methyl
22. Coumaphos
20
40
21
22
60 min
Ordering information
Length
10m
25m
30m
50m
60m
726490.10
726465.25
726465.50
0.25m film
726470.30
726470.60
0.25m film
0.35m film
1.00m film
726480.30
726481.30
726482.30
726480.60
726481.60
726482.60
1.00m film
726483.30
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
250
info@mn-net.com
CH3
O
Si
Si
CH3
(CH2)3 m
CN
Carrier gas:
Temperature:
USP G43
Injection:
Detector:
Peaks:
1. Propyzamide
2. Vinclozolin
3. Bromophos-ethyl
4. 2,4-DDT
5. Brompropylate
4
3
2
Column:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
1. PCB-28
2. PCB-52
3. PCB-128
4. PCB-153
5. PCB-138
6. PCB-180
1
2
20
min
40
60
20
min
40
60
Ordering information
Length
25m
30m
50m
60m
0.25m film
726771.25
726771.30
726771.50
726771.60
0.25m film
1.00m film
726777.25
726777.30
726780.30
726780.50
726777.60
726780.60
1.00m film
726783.25
Column:
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.
Columns have fused ends or are sealed with septa, to protect them from atmospheric oxygen. A standard test mixture is included with every column.
www.mn-net.com
251
CH3
O
Si
CH3
(CH2)3 m
Si
CN
OPTIMA 624 LB
USP G43
Excellent Low Bleed columns for halogenated hydrocarbons, volatiles, aromatic compounds, solvents etc.
Column:
Injection:
Carrier gas:
Temperature:
OPTIMA 624 LB, 1.8m film, 30m x 0.32mm ID; retention gap Phe-Sil 0.5m x 0.53mm
1L (10ppm per substance in acetone),
15 17
1
14
cold on-column
1.1bar He
45C (3min) 150C (6C/min)
8
300C (18C/min), 20min 300C
16
13
FID 280C
Detector:
Peaks:
1. Acetone
2. Ethyl acetate
3. Tetrahydrofuran
4. Cyclohexane
5. 2-Methyl-2-butanol
6. 1-Butanol
7. Pyridine
8. Toluene
9. Dimethylformamide
10. Dimethylsulfoxide
11. Decane
12. 1-Octanol
13. Acetophenone
14. Butyrophenone
15. Heptanophenone
16. 5-Methoxyindole
17. Dibenzylamine
18. Methyl eicosanoate
19. Methyl cis-13-docosenoate
20. Methyl docosanoate
MN Appl. No. 212520
45
3 6
11 12
18
19
20
9 10
10
20
30
40 min
Ordering information
OPTIMA 624
Length
25m
50m
60m
726785.30
726785.50
726785.60
726787.30
726787.50
726787.60
726784.25
1.40m film
726785.25
1.80m film
726787.25
3.00m film
726789.25
OPTIMA 624 LB
30m
726789.30
726786.30
726786.50
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
On request, all columns can be supplied on a 5 inch (13cm) cage for the Agilent GC 6850. To order, please add an E at the
end of the REF number (e.g., 726470.30E)
252
info@mn-net.com
CH3
O
(CH2)3 m
Si
Si
CH3
CN
USP G46
Column:
OPTIMA 1701, 0.25m film, 25m x 0.32mm ID
Injection:
1L, split 1: 40
Carrier gas:
0.6bar N2
Temperature:
60C 120C, 4C/min
Detector:
FID 260C
Peaks:
1. Benzene
2. Toluene
3. Ethylbenzene
4. p-Xylene
5. o-Xylene
6. Phenol
7. 2-Methylphenol
8. 2,6-Dimethylphenol
9. 4-Methylphenol
10. 2,4-Dimethylphenol
11. 2,4,6-Trimethylphenol
4
9
3
6 7
10
10
11
15 min
Ordering information
Length
10m
15m
0.25m film
0.50m film
1.00m film
726058.10
0.25m
0.35m
0.50m
1.00m
film
film
film
film
726318.10
1.00m film
2.00m film
726545.10
30m
726841.25
0.20m film
25m
50m
60m
726841.50
726058.15
726058.25
726058.30
726064.30
726965.30
726058.50
726058.60
726064.60
726318.15
726318.25
726824.25
726320.25
726929.25
726318.30
726824.30
726320.30
726929.30
726318.50
726824.50
726320.50
726929.50
726318.60
726824.60
726320.60
726929.60
726545.15
726735.15
726545.25
726735.25
726545.30
726735.30
726735.50
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
www.mn-net.com
253
silarylene phase
Si
O
m
CH3
CH3
Si
Si
CH3
CH3
CH3
O
Si
n
CH3
USP G42
Column:
Injection:
Carrier gas:
Temperature:
Detector:
1
7 8
10
11
12
14
13
15
14
10
12
Peaks:
1. Naphthalene
2. Acenaphthylene
3. Acenaphthene
4. Fluorene
5. Phenanthrene
6. Anthracene
7. Fluoranthene
8. Pyrene
9. Benz[a]anthracene
10. Chrysene
11. Benzo[b]fluoranthene
12. Benzo[k]fluoranthene
13. Benzo[a]pyrene
14. Indeno[1,2,3-cd]pyrene
15. Dibenz[ah]anthracene
16. Benzo[ghi]perylene
14
16
18
20
22
24
26
28
16
30
min
Ordering information
Length
30m
60m
0.25m film
726154.30
726154.60
0.25m film
726157.30
726157.60
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.
Columns have fused ends or are sealed with septa, to protect them from atmospheric oxygen. A standard test mixture is included with every column.
254
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CH3
Si
Recommended application:
steroids, pesticides, drug analyses
USP G3
Sample:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
1. Dichlorphos
2. Naled
3. Vinclozolin
4. Chlorthalonil
5. Chlorpyrifos
6. Dichlofluanid
7. Procymidon
8. Captan
9. Folpet
10. Carbophenothion
11. Iprodion
12. Captafol
13. Coumaphos
10
13
34 6 7
89
10
1112
20
30 min
Ordering information
Length
10m
12m
15m
25m
30m
50m
60m
726848.10
726065.12
726065.25
726066.25
726022.15
726742.25
726022.25
726067.25
726742.30
726022.30
726067.30
726742.50
726022.50
726067.50
726742.60
726022.60
726067.60
726351.25
726757.25
726744.25
726755.30
726351.30
726757.30
726744.30
726351.50
726757.50
726744.50
726351.60
726757.60
726744.60
726747.25
726747.30
film
film
film
film
726747.10
726747.15
726065.50
726066.50
Analysis of pesticides
Column:
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
On request, all columns can be supplied on a 5 inch (13cm) cage for the Agilent GC 6850. To order, please add an E at the
end of the REF number (e.g., 726470.30E)
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255
silarylene phase
CH3
CH3
Si
Si
CH3
CH3 m
Si
Column:
Sample:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
1. Phenol
2. 2-Chlorophenol
3. 2,4-Dimethylphenol
4. 2-Nitrophenol
5. 2,4-Dichlorophenol
6. 4-Chloro-3-methylphenol
7. 2,4,6-Trichlorophenol
8. 4-Nitrophenol
9. 2,4-Dinitrophenol
10. 2-Methyl-4,6-dinitrophenol
11. Pentachlorophenol
Analysis of phenols
8
10
7
1
2
11
45
12
14
min
20
Ordering information
Length
30m
60m
0.25m film
726162.30
726162.60
0.25m film
726165.30
726165.60
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.
Columns have fused ends or are sealed with septa, to protect them from atmospheric oxygen. A standard test mixture is included with every column.
To prolong column life, even at highly contaminated or matrix-containing samples, MN offers the option to add an integrated
guard column. All capillary columns are available with a 10m guard column with respective deactivation. To order, please add
V1 at the end of the REF number. Guard column combinations with other lengths, IDs or different deactivation are available on
request.
256
info@mn-net.com
CH3
Si
(CH2)2 n
CF3
10
6
20 min
Column:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
1. Benzene
2. Toluene
3. Ethylbenzene
4. p-Xylene
5. m-Xylene
6. o-Xylene
Ordering information
Length
15m
25m
30m
50m
60m
0.25m film
0.50m film
726871.15
726871.25
726871.30
726874.30
726871.50
726874.50
726871.60
726874.60
0.25m film
0.50m film
726877.15
726877.30
726880.30
726877.50
726880.50
726877.60
726880.60
726880.25
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
On request, all columns can be supplied on a 5 inch (13cm) cage for the Agilent GC 6850. To order, please add an E at the
end of the REF number (e.g., 726470.30E)
www.mn-net.com
257
Si
(CH2)3
Column:
Injection:
Temperature:
Detector:
Peaks:
1. C4:0
2. C5:0
3. C6:0
4. C8:0
5. C10:0
6. C11:0
7. C12:0
8. C13:0
9. C13:1
10. C14:0
11. C14:1
12. C15:0
13. C15:1
14. C16:0
15. C16:1
16. C17:0
17. C17:1
Si
CN
CH3
m=n
18. C18:0
19. C18:1
20. C18:2
21. C18:3
22. C19:0
23. C20:0
24. C20:1
25. C20:2
26. C20:4
27. C20:3
28. C20:5
29. C22:0
30. C22:1
31. C22:2
32. C22:6
33. C24:0
34. C24:1
20
18
19
14
18 20
19
16
12
3 4
2
15
22
21
9 1113 17
24
25
10
22
28
30
23 26 3132
27 29 3334
2
5
min
1112
16
17
21
24
25
23 26
27
32
10
20
10m
15m
25m
30m
50m
60m
726118.15
726118.25
726118.30
726118.50
726118.60
726352.25
726352.30
726352.50
726352.60
10
20
min
Ordering information
Length
726080.10
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
On request, all columns can be supplied on a 5 inch (13cm) cage for the Agilent GC 6850. To order, please add an E at the
end of the REF number (e.g., 726470.30E)
258
info@mn-net.com
Si
CH3
O
Si
(CH2)3 m
CH3
CN
12
10
8
7
14
13
29
28
11
9
24
19
6
4
22
23
17
16 18
15
30
25
26
27
20
21
31
3
2
10
20
30
40
50 min
30m
50m
60m
726089.30
726090.30
726089.50
726089.60
726090.60
726091.30
726095.30
726096.30
726091.50
726091.60
726095.60
726096.60
Ordering information
Length
25m
726091.25
Column:
Sample:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
1. C4:0
2. C5:0
3. C8:0
4. C10:0
5. C11:0
6. C12:0
7. C13:0
8. C14:0
9. C14:1
10. C15:0
11. C15:1
12. C16:0
13. C16:1
14. C17:0
15. C17:1
16. C18:0
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.
Columns have fused ends or are sealed with septa, to protect them from atmospheric oxygen. A standard test mixture is included with every column.
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259
OH
Similar phases:
PERMABOND CW 20 M (page
265), DB-Wax, Supelcowax,
HPWax, HP-INNOWAX, RtxWax,
CP-Wax 52 CB, Stabilwax, 007CW, BP20, AT-Wax, ZB-Wax
USP G16
Column:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
1. Decane
2. Undecane
3. Octanol
4. Methyl decanoate
5. Dicyclohexylamine
6. Methyl undecanoate
7. Methyl dodecanoate
8. 2,6-Dimethylaniline
9. 2,6-Dimethylphenol
10
15
89
20 min
Ordering information
Length
25m
30m
50m
60m
0.25m film
726600.25
726600.30
726600.50
726600.60
0.25m film
0.50m film
726321.25
726296.25
726321.30
726296.30
726321.50
726296.50
726321.60
726296.60
1.00m film
2.00m film
726549.25
726549.30
726548.30
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
On request, all columns can be supplied on a 5 inch (13cm) cage for the Agilent GC 6850. To order, please add an E at the
end of the REF number (e.g., 726470.30E)
260
info@mn-net.com
OH
n
USP G16
Recommended application:
broad range of application, e.g., for solvents and alcohols, suitable for aqueous solutions
Columns:
Injection:
Carrier gas:
Temperature:
W
NE
Alcohols
Detector:
Peaks:
1. Methanol
2. 2-Propanol
3. Ethanol
4. 1-Propanol
5. 2-Methyl-1-propanol
6. 1-Butanol
7. 4-Methyl-2-pentanol
8. 1-Pentanol
9. 2-Methyl-1-Pentanol
10. 1-Hexanol
11. Cyclohexanol
12. 1-Heptanol
9
8
10
11
12
3
2
12
min
Ordering information
Length
30m
60m
0.25m film
0.50m film
726380.30
726381.30
726380.60
726381.60
0.25m film
0.50m film
726382.30
726383.30
726382.60
726383.60
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.
Columns have fused ends or are sealed with septa, to protect them from atmospheric oxygen. A standard test mixture is included with every column.
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261
(OCH2CH2)m
O2N
O
n
Recommended application:
fatty acid methyl esters (FAMEs), free carboxylic acids
FAME test
Column:
Carrier gas:
Temperature:
Injection:
Detector:
Peaks:
1. C4
2. C6
3. C8
4. C10
5. C12
6. C14
7. C16
8. C18
9. C18:1 cis/trans
10. C18:2
11. C18:3
12. C20
13. C22
14. C22:1
15. C24
9
7
8
10
20
30
11
10 12
40
13
14
50
15
60 min
Ordering information
Length
25m
30m
50m
60m
0.25m film
726116.25
726116.30
726116.50
726116.60
0.25m film
0.50m film
726341.25
726344.25
726341.30
726344.30
726341.50
726344.50
726341.60
0.50m film
1.00m film
726346.25
10m
726180.10
726345.30
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
To prolong column life, even at highly contaminated or matrix-containing samples, MN offers the option to add an integrated
guard column. All capillary columns are available with a 10m guard column with respective deactivation. To order, please add
V1 at the end of the REF number. Guard column combinations with other lengths, IDs or different deactivation are available on
request.
262
info@mn-net.com
Polar phase
O
(OCH2CH2)m
O2N
!
W
NE
Recommended application:
FAMEs, free carboxylic acids
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
Methyl esters of:
1. Caproic acid (C6:0)
2. Caprylic acid (C8:0)
3. Capric acid (C10:0)
4. Lauric acid (C12:0)
5. Myristic acid (C14:0)
6. Palmitic acid (C16:0)
7. Palmitoleic acid (C16:1)
8. Stearic acid (C18:0)
9. Oleic acid (C18:1 cis)
10. Linoleic acid (C18:2 cis)
11. Nonadecanoic acid (C19:0)
12. Linolenic acid (C18:3)
13. Arachidic acid (C20:0)
14. Behenic acid (C22:0)
15. Erucic acid (C22:1 cis)
16. Lignoceric acid (C24:0)
17. Nervonic acid (C24:1 cis)
5
4
3
11
13
2
14
10
12
15
16
10
12
17
14
min
Column:
Ordering information
Length
30m
60m
0.25m film
0.50m film
726241.30
726242.30
726241.60
726242.60
0.25m film
0.50m film
726243.30
726246.30
726243.60
726246.60
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
On request, all columns can be supplied on a 5 inch (13cm) cage for the Agilent GC 6850. To order, please add an E at the
end of the REF number (e.g., 726470.30E)
www.mn-net.com
263
100% dimethylpolysiloxane
Nonpolar phase
CH3
Si
CH3
Ordering information
Length
25m
50m
0.25m film
723052.25
723052.50
0.25m film
0.50m film
723306.25
723308.50
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
PERMABOND SE-52
Nonpolar phase
CH3
CH3
O
Si
Si
CH3
Ordering information
Length
25m
0.25m film
723054.25
0.25m film
0.50m film
723310.25
723312.25
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.
Columns have fused ends or are sealed with septa, to protect them from atmospheric oxygen. A standard test mixture is included with every column.
264
info@mn-net.com
OH
n
USP G16
Ordering information
Length
10m
50m
60m
0.10m film
723064.10
0.25m film
723060.10
723060.25
723060.30
723060.50
723060.60
0.25m film
0.35m film
0.50m film
723321.10
723827.10
723296.10
723321.25
723827.25
723296.25
723321.30
723321.50
723827.50
723296.50
723321.60
0.50m film
723515.10
1.00m film
723549.10
2.00m film
723517.10
In addition to this standard program we will be happy to
Polar phase
O
(OCH2CH2)m
O2N
723296.30
723296.60
723515.25
723549.25
723549.30
723517.25
723517.30
supply columns custom-made to your specifications.
PERMABOND FFAP
30m
O
n
Ordering information
Length
10m
20m
0.10m film
0.25m film
723180.10
723181.10
723180.20
0.10m film
0.25m film
723116.10
film
film
film
film
723830.10
723344.10
25m
30m
50m
60m
723936.25
723116.25
723116.30
723936.50
723116.50
723116.60
723356.25
723341.25
723830.25
723344.25
723341.30
723344.30
723356.50
723341.50
723830.50
723344.50
25m
723341.60
723344.60
1.00m film
723555.10
723555.25
723555.50
In addition to this standard program we will be happy to supply columns custom-made to your specifications.
www.mn-net.com
265
Chiral separations
Page
Fast GC
267
Enantiomer separation
Biodiesel
cyclodextrin phases
268
270
272
272
272
Triglycerides
OPTIMA 1-TG
OPTIMA 17-TG
274
High temperature GC
OPTIMA 5 HT
275
OPTIMA 5 Amine
FS-CW 20 M-AM
276
277
PERMABOND P-100
277
polyfunctional amines
amine separations
266
OPTIMA BioDiesel M
OPTIMA BioDiesel F
OPTIMA BioDiesel G
Amines
methanol analysis
FAME analysis
glycerol and triglycerides
FS-LIPODEX A, FS-LIPODEX B
FS-LIPODEX C, FS-LIPODEX D
FS-LIPODEX E, FS-LIPODEX G
FS-HYDRODEX -PM, FS-HYDRODEX -3 P
FS-HYDRODEX -6TBDM
FS-HYDRODEX -TBDAc, FS-HYDRODEX -TBDAc
278
PERMABOND Silane
279
PERMABOND CW 20 M-DEG
279
info@mn-net.com
Comparison of a separation on a 50m standard capillary with separation on a 10m fast GC column
A) Fast GC column
Column: OPTIMA 5, 0.1m film, 10m x 0.1mm ID
injection 1L, split 1: 40, carrier gas 0.75bar He
Both separations: temperature
80C 320C (10min), 8C/min,
detector: FID
While maintaining the temperature
program and halving the pressure
2
a time saving of 30% results with
4
identical separation efficiency
Peaks:
1
1. Octanol
3
2. Undecane
6 7
3. Dimethylaniline
5
4. Dodecane
5. Decylamine
6. Methyl decanoate
7. Methyl undecanoate
8. Henicosane
9. Docosane
10. Tricosane
MN Appl. No. 211260 0
Ordering information
Phase
OPTIMA 1
B) standard GC column
Column: OPTIMA 5, 0.25m film, 50m x 0.25mm ID
injection 1L, split 1: 35, carrier gas 1.5bar He
1
7
8
9
10
89
10
10
Max. temperature
340/360 C
OPTIMA 5
OPTIMA -3
OPTIMA -6
OPTIMA 17
OPTIMA 225
OPTIMA FFAP
PERMABOND CW 20 M
PERMABOND FFAP
340/360 C
340/360 C
340/360 C
320/340 C
260/280 C
250/260 C
OPTIMA 5 Amine
FS-CW 20 M-AM
FS-LIPODEX E
FS-HYDRODEX -6TBDM
300/320 C
220/240 C
220/240 C
220/240 C
200/220 C
230/250 C
20 min
ID [mm]
0.10
0.10
0.10
0.10
0.10
0.10
0.10
0.10
0.10
0.10
0.10
0.10
0.10
0.10
0.10
10
20
REF (10m)
726024.10
726846.10
726410.10
726490.10
726848.10
726080.10
726180.10
723064.10
723180.10
723181.10
30
min
REF (20m)
726024.20
726025.20
System requirements for Fast GC: high sensitivity detectors with small
volume and very short response time, as well as very rapid data acquisition and processing small inner diameters result in high column inlet
pressures and a lower volume flow of the mobile phase: very fast injection of very small samples against a high pressure amount of sample,
which can be injected, is limited by the inner diameter and the thin film
726410.20
723180.20
726361.10
733111.10
723382.10
723383.10
In addition to this standard program, all MN GC phases can be custom-made as fast GC columns.
www.mn-net.com
267
Base material: cyclic oligosaccharides consisting of six (-cyclodextrin), seven (-cyclodextrin) or eight
(-cyclodextrin) glucose units bonded through -1,4-linkages
Regioselective alkylation and/or acylation of the hydroxyl groups leads to lipophilic phases with varying
enantioselectivity, which are well suited for GC enantiomer analyses
Important advantage: many compounds can be analyzed without derivatization (however, for certain substances enantioselectivity can be favorably influenced by formation of derivatives)
A large number of separations have been achieved, however, it is not possible to make a general prediction, which phase could solve a given separation task. Even for compounds with small structural differences or within homologous series the enantiodifferentiation can be quite different. The descriptions below
list some of the typical separations possible with individual phases.
Water as solvent is strictly forbidden for all cyclodextrin phases. We recommend to dry the sample with our
CHROMAFIX Dry cartridges (page 47) and to dissolve it in an appropriate nonpolar solvent in any case.
LIPODEX A
hexakis-(2,3,6-tri-O-pentyl)--cyclodextrin
LIPODEX B
hexakis-(2,6-di-O-pentyl-3-O-acetyl)--cyclodextrin
Recommended application: lactones, diols (cyclic carbonates), aminols, aldols (O-TFA), glycerol derivatives
(cyclic carbonates)
LIPODEX C
octakis-(2,3-di-O-pentyl-6-O-methyl)--cyclodextrin
268
octakis-(2,6-di-O-pentyl-3-O-butyryl)--cyclodextrin
LIPODEX G
heptakis-(2,6-di-O-pentyl-3-O-acetyl)--cyclodextrin
LIPODEX E
heptakis-(2,3,6-tri-O-pentyl)--cyclodextrin
Recommended application: alcohols, cyanhydrins, olefins, hydroxycarboxylic acid esters, alkyl halides
LIPODEX D
info@mn-net.com
Column:
FS-LIPODEX E, 25m x 0.25mm ID
Volume:
1L, split ~ 1: 100
Carrier gas:
60kPa H2
Temperature:
90 190C, 4C/min
Detector:
FID 250C
Peaks:
(D is eluted before L except for proline: L before D)
1. Alanine
2. Valine
3. Leucine
4. Proline
5. Aspartic acid
6. Phenylalanine
2
1
4
5
16
10
20 min
67
Mentha arvensis
(China)
10
9
15
8
Mentha piperita
(Idaho)
1
12
11
13
14
10
18
Standard
17
20
40
60
min
Ordering information
Length
all columns 0.4mm OD
FS-LIPODEX
FS-LIPODEX
FS-LIPODEX
FS-LIPODEX
FS-LIPODEX
FS-LIPODEX
A
B
C
D
E
G
www.mn-net.com
10m
0.10mm ID
723382.10
25m
0.25mm ID
723360.25
723362.25
723364.25
723366.25
723368.25
723379.25
50m
0.25mm ID
723360.50
723362.50
723364.50
723366.50
723368.50
723379.50
269
Cyclodextrin derivatives with high melting point: for GC enantiomer separation diluted with polysiloxanes
HYDRODEX -PM
heptakis-(2,3,6-tri-O-methyl)--cyclodextrin (CD)
Recommended application: hydroxycarboxylic acid esters, alcohols, diols, olefins, lactones, acetals
HYDRODEX -3P
heptakis-(2,6-di-O-methyl-3-O-pentyl)--CD
Recommended application: terpenes, dienes, allenes, terpene alcohols, 1,2-epoxyalkanes, carboxylic acids (esters),
hydroxycarboxylic acid esters, pharmaceuticals, pesticides
HYDRODEX -6TBDM
heptakis-(2,3-di-O-methyl-6-O-t-butyldimethyl-silyl)--CD
HYDRODEX -TBDAc
heptakis-(2,3-di-O-acetyl-6-O-t-butyldimethyl-silyl)--CD
Column:
Injection:
Carrier gas:
Temperature:
Detector:
COOH
H 3C
2. Flurbiprofen
COOH
CH3
H 3C
COOH
3
2
1
SR
10
OH
CH CO2CH3
Cl
5. Ketoprofen
CH3
Cl
CH3O
CH3
3. Fenoprofen
min
20
MN Appl. No. 202542
270
COOH
CH3
10
4
5
15
20
30
40
50 min
info@mn-net.com
octakis-(2,3-di-O-acetyl-6-O-t-butyldimethyl-silyl)--CD
Recommended application: cyclic ketones, aromatic ketones, oxiranes, aromatic esters, aromatic amides etc.
HYDRODEX -DiMOM
octakis-(2,3-di-O-methoxymethyl-6-O-t-butyldimethylsilyl)--CD
Phase diluted with optimized polysiloxane
Column:
FS-HYDRODEX -TBDAc, 50m x 0.25mm ID
Injector:
220C
Carrier gas:
1.2bar H2
Temperature:
125C
Detector:
FID 220C
Peaks:
1. Fenchone (1.5mg/mL)
2. Menthone (0.5mg/mL)
3. Menthol (2mg/mL)
OH
3
1
2
2
10
20
30
min
10
min
12
Column:
FS-HYDRODEX -TBDAc, 50m x 0.25mm ID
Injection:
1L, 1: 1000 in CH2Cl2, split 25mL/min
Carrier gas:
1.5bar H2
Temperature:
100C
Detector:
FID 220C
Peaks:
1. (R)/(S)-Citronellal
2. (S)/(R)-Citronellal
3. (S)-Citronellol
4. (R)-Citronellol
Ordering information
all columns 0.4mm OD
FS-HYDRODEX
FS-HYDRODEX
FS-HYDRODEX
FS-HYDRODEX
FS-HYDRODEX
FS-HYDRODEX
Length
-PM
-3P
-6TBDM
-TBDAc
-TBDAc
-DiMOM
www.mn-net.com
10m
0.10mm ID
723383.10
25m
0.25mm ID
50m
0.25mm ID
723358.25
723358.50
723370.25
723370.50
723381.25
723381.50
723387.25
723387.50
723384.25
723388.25
723384.50
723388.50
271
OPTIMA BioDiesel M
for analysis of methanol in accordance with DIN EN 14110
A
1
0.8
0.6
0.4
0.2
0
0.2
0.4
OPTIMA BioDiesel F
for analysis of FAMEs in accordance with DIN EN 14103:2011
The analysis of biodiesel requires separation of typical FAMEs between myristic acid (C14) and nervonic acid (C24:1) methyl esters.
This analysis is possible on OPTIMA BioDiesel F in only 22min. Additionally, linolenic acid methyl ester can be determined due to the
good resolution.
The extended standard DIN EN 14103:2011 also covers smaller
FAMEs starting from C6 (see application 214510 on opposite page).
Change of the internal standard from C17 to C19 also allows the
analysis of animal fats.
Similar phases: Select Biodiesel for FAME, Trace TR-BioDiesel (F)
Max. temperature for isothermal operation 240C, max. temperature for short isotherms in a temperature program 250C
OPTIMA BioDiesel G
for analysis of glycerol and glycerides in accordance with DIN
EN 14105
Max. temperature for isothermal operation 380C, max. temperature for short isotherms in a temperature program 400C
272
info@mn-net.com
% CH3OH
8 10
11
12
13
1
14 15
16
10
12
14
16 min
Column:
Sample:
Injection:
Carrier gas:
Temperature:
OPTIMA BioDiesel G,
10m x 0.25mm ID
A) standard in n-heptane
B) biodiesel
2L, 350C, split 1: 2.6
CIS (15C 350C, 12C/s)
0.8bar H2
50C (3.5min) 180C, 15C/min
280C, 7C/min
370C (10min), 10C/min
FID 380C
Detector:
Peaks:
1. Glycerol (TMS)
2. Butanetriol (TMS), IS
3. Monoolein = glycerol monooleate (TMS)
+ monoacylglycerides
4. Tricaprin (glycerol tricaprate), IS
5. Diolein = glycerol dioleate (TMS)
+ diacylglycerides
6. Triolein = glycerol trioleate
+ triacylglycerides
1
A
Column:
Sample:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
1. C6:0
2. C8:0
3. C10:0
4. C12:0
5. C14:0
6. C16:0
7. C16:1
8. C18:0
9. C18:1
10. C18:2
11. C19:0, int. st.
12. C18:3
13. C20:0
14. C22:0
15. C22:1
16. C24:0
B
MN Appl. No. 213640
10
20
30
min
Ordering information
OPTIMA BioDiesel M
Length
10m
30m
726905.30
726900.30
OPTIMA BioDiesel F
OPTIMA BioDiesel G
www.mn-net.com
726903.10
273
OPTIMA 1-TG
100% dimethylpolysiloxane
offers separation according to carbon number
Similar phases:
SPB-1 TG, DB-1 HT, 400-1 HT, HT-5
USP G1/G2/G38
OPTIMA 17-TG
USP G3
Column:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
1. Cholesterol
2. T-30
3. T-34
4. T-38
5. T-42
6. T-46
7. T-50
8. T-54
4
7
3
6
5
160
0
278
10
MN Appl. No. 201790
328
20
370
30
40
Ordering information
OPTIMA 1-TG
OPTIMA 17-TG
274
Length
10m
25m
726133.10
726132.10
726133.25
726132.25
726131.10
726131.25
info@mn-net.com
min
Recommended application: for simulated distillation, hydrocarbon, fuel and oil analysis,
high-boiling analytes
USP G27/G36
Column:
Sample:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
1. Decane (C10)
2. Tetracontane (C40)
10
12
min
14
Ordering information
Length
15m
30m
0.10m film
0.25m film
726102.15
726106.15
726102.30
726106.30
0.10m film
0.25m film
726104.15
726108.15
726104.30
726108.30
www.mn-net.com
275
USP G27/G36
Column:
OPTIMA 5 Amine, 0.5m film, 30m x 0.25mm ID
Injection:
1L, split 1: 100
Carrier gas:
0.6bar H2
Temperature:
100C (3min) 280C, 10C/min
Detector:
FID 280C
Peaks:
1. Diethylamine
2. Di-isopropylamine
3. Triethylamine
4. Di-n-propylamine
5. Di-n-butylamine
6. Tri-n-propylamine
7. Di-isobutylamine
8. Tri-n-butylamine
9. Di-isohexylamine
10. Dicyclohexylamine
11. Dibenzylamine
12. Tri-n-hexylamine
12
10
23
11
6 7
12
16
Ordering information
Length
25m
30m
726361.10
726355.25
0.50m film
1.00m film
726354.30
726358.30
0.25m film
1.00m film
1.50m film
726360.30
726353.30
726356.30
1.00m film
3.00m film
726359.30
726357.30
276
10m
info@mn-net.com
min 20
USP G16
Ordering information
Length
25m
50m
0.25m film
733110.25
733110.50
0.25m film
0.35m film
733299.25
733299.50
733442.50
1.00m film
733551.25
10m
733111.10
PERMABOND P-100
USP G1/G2/G38
Ordering information
Length
www.mn-net.com
100m
723890.100
277
Max. temperature for isothermal operation 300C, max. temperature for short isotherms in a temperature program 320C
For the analysis of halogenated hydrocarbons, we recommend our optimized column PERMABOND SE-54HKW at 25 or 50m length with our approved polysiloxane phase SE-54. As an alternative, or to verify
analytical results, the OPTIMA 624 has proven itself
as advantageous, especially for the determination of
1,1,2-trichlorotrifluoroethane (F113) along with di-
chloromethane. Both phases are also suited for the determination of vinyl chloride as well as for the separation of cis/trans isomers of 1,2-dichloroethene. The
high film thickness secures a high capacity and an
outstanding resolution. For GC/MS coupling, we recommend OPTIMA 624 LB or OPTIMA 624 with 0.2 or
0.25mm ID.
Column:
PERMABOND SE-54-HKW, 50m x 0.32mm ID
Injection:
1L, split ~ 1: 30
Carrier gas:
0.9bar He
Temperature:
35C (25min) 160C (5min), 10C/min
Detector:
ECD 300C
Peaks:
1. Dichloromethane (795ng/mL)
2. Trichloromethane (75ng/mL)
3. 1,1,1-Trichloroethane (67ng/mL)
4. 1,2-Dichloroethane (100ng/mL)
5. Tetrachloromethane (15.9ng/mL)
6. Trichloroethene (14.6ng/mL)
7. Bromodichloromethane (20ng/mL)
8. Dibromochloromethane (122ng/mL)
9. Tetrachloroethene (81ng/mL)
10. Tribromomethane (28.9ng/mL)
MN Appl. No. 2124880
10
20
10
30
40 min
Column:
OPTIMA 624, 50m x 0.25mm ID
Injection:
1L, split 50mL/min
Carrier gas:
0.9mL/min He (constant flow)
Temperature:
40C (5min) 160C, 10C/min
Detector:
MSD 5971
Peaks:
1. Vinyl chloride
13. Trichloroethene
2. Trichlorofluoromethane (F 11)
14. Bromodichloromethane
3. Pentane
15. Toluene
4. 1,1,2-Trichlorotrifluoroethane (F 113) 16. Tetrachloroethene
5. Dichloromethane
17. Dibromochloromethane
6. trans-1,2-Dichloroethene
18. Chlorobenzene
7. Hexane
19. Ethylbenzene
8. cis-1,2-Dichloroethene
20. m- + p-Xylene
9. Trichloromethane
21. o-Xylene
1
10. 1,1,1-Trichloroethane
22. Tribromomethane
4
11. Tetrachloromethane
23. Bromobenzene
12. 1,2-Dichloroethane + benzene
17
20 22
16
18
19
21
13
11
4
8
5 6
2
3
6
14
15
910 12
7
8
10
12
14
16 min 18
MN Appl. No. 200160
Ordering information
Length
278
23
25m
50m
723945.25
723945.50
info@mn-net.com
Developed especially for the analysis of monomeric silanes and chlorosilanes (not for the separation of trimethylsilyl derivatives)
Ordering information
Length
0.32mm ID (0.5mm OD)
0.53mm ID (0.8mm OD)
25m
50m
723409.50
723411.25
Column:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
DEG standard
1. 1,4-Butanediol
2. Diethylene
glycol
3. Glycerol
PERMABOND CW 20 M-DEG,
25m x 0.25mm ID
0.5L, split ~1:40
1.2bar N2
80C 200C, 10C/min
FID 260C
1
4
3
1
7
4
min 10
10
15 min
MN Appl. No. 201500
PERMABOND CW 20 M-DEG
Chloromethylsilanes
Column:
PERMABOND Silane, 50m x 0.32mm ID
Injection:
0.5L gas, split 80mL/min
Carrier gas:
1mL/min He (constant flow)
Temperature:
50C 100C, 5C/min
Detector:
MSD 5971
Peaks:
23
1. Tetramethylsilane
2. Dichloromethane
3. Tetrachlorosilane
4. Chlorotrimethylsilane
5
5. Methyltrichlorosilane
6. Dichlorodimethylsilane
6
7. Hexamethyldisiloxane
Ordering information
Length
0.25mm ID (0.4mm OD)
0.32mm ID (0.5mm OD)
www.mn-net.com
0.25m film
0.25m film
25m
723063.25
723327.25
279
Ordering information
Length
0.025mm
0.05mm
0.075mm
0.10mm
ID
ID
ID
ID
(0.4mm
(0.4mm
(0.4mm
(0.4mm
OD)
OD)
OD)
OD)
1m
(pack of 3)
10m
(pack of 1)
723793.1
723790.1
723791.1
723792.1
723793.2
723790.2
723791.2
723792.2
Untreated capillaries
0.20mm
0.25mm
0.32mm
0.53mm
ID
ID
ID
ID
(0.4mm
(0.4mm
(0.5mm
(0.8mm
723148.10
723101.10
723151.10
723501.10
OD)
OD)
OD)
OD)
25m
(pack of 1)
723148.25
723101.25
723151.25
723501.25
Recommended applications:
Ordering information
Length
10m
25m
723106.10
723346.10
723558.10
723106.25
723346.25
723558.25
723108.10
723348.10
723560.10
723108.25
723348.25
723560.25
723105.10
723349.10
723562.10
723105.25
723349.25
723562.25
280
info@mn-net.com
Max. temperatures: for CW retention gaps 250C, for Me-Sil and Phe-Sil retention gaps 320C
Retention gaps can also be used as transfer lines or precolumns (contamination capacity about 510g).
Ordering information
Length
10m
25m
723706.10
723707.10
723708.10
723706.25
723707.25
723708.25
723709.10
723710.10
723711.10
723709.25
723710.25
723711.25
723712.10
723713.10
723714.10
723712.25
723713.25
723714.25
For a considerably longer lifetime, even for contaminated or matrix-containing samples, MN offers the option of integrated
precolumns. All capillary columns are available with a 10m guard column with matched deactivation. For ordering, please add
V1 at the end of the REF number. Guard column combinations with other lengths, IDs or different deactivation are available on
request.
www.mn-net.com
281
To improve volatility, increase thermal stability or to achieve a lower limit of detection in gas chromatography
Prerequisite: quantitative, rapid and reproducible formation of only one derivative
Halogen atoms inserted by derivatization, e.g., trifluoroacetates, allow the specific detection in an ECD
with the advantage of high sensitivity.
Specific derivatizations may influence elution orders and fragmentation patterns in a MS.
REF
701952
701950
701951
701953
Reagents for GC
Acylation kit
Alkylation kit
Silylation kit
Alcohols,
Phenols
ROH
sterically hindered
Amines
primary, secondary
hydrochlorides
Derivative
acylation
alkylation
silylation
silylation
acylation
ROCOR
ROR
ROTMS
RNRTMS
RNRCOR
RCONHCOR
RCH(NHTMS)COOTMS
silylation
ROTMS
silylation
silylation
RNRTMS
not stable
RCH(NHCO-R)COOR
Carboxylic acids
alkylation (a)
+ acylation (b)
silylation
salts
alkylation
silylation
Carbohydrates
silylation
Amides
Amino acids
(fatty acids)
Steroids
282
Method
acylation
silylation
acylation
silylation
acylation
RCOOTMS
susceptible to hydrolysis
RCOOR
RCOOTMS
susceptible to hydrolysis
Recommended reagents
MSTFA
a) MeOH/TMCS, TMSH
b) TFAA, HFBA, MBTFA, MBHFBA
BSA, MSTFA, MSHFBA, TMCS, TSIM,
SILYL-2110, SILYL-21, Silyl 1139
DMF-DMA, MeOH/TMCS (1 M), TMSH
TMCS
info@mn-net.com
Pentafluorobenzoyl chloride
PFBC:
C6F5COCl
Anhydrides
By-products of acylation with anhydrides: corresponding acids
excess reagent and the acid formed are to be removed
Bisacylamides
By-products: corresponding neutral acylamides: high volatility easily removed; due to the neutral conditions and their favorable chromatographic characteristics, the removal of surplus bisacylamides and their
by-products is often not necessary. Therefore, the sample preparation is much easier.
N-methyl-bis(trifluoroacetamide)
MBTFA: CF3CON(CH3)COCF3
N-methyl-bis(heptafluorobutyramide)
MBHFBA: C3F7CON(CH3)COC3F7
Reagents for GC
Acyl halides
By-product of acylation with acyl halides: corresponding hydrohalic acids
excess of reagent and acid have to be removed or trapped by a suitable base (e.g., pyridine)
Ordering information
Substance
HFBA*
MBTFA*
MBHFBA*
PFBC*
TFAA*
10 x 1mL
701420.101
701120.101
20 x 1mL
701110.201
701410.201
701420.201
Packing unit
1 x 10mL
701110.110
701410.110
5 x 10mL
701110.510
701410.510
701130.110
701130.510
* These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.
Due to their purpose, derivatization reagents are very reactive substances. For this reason, they should be stored cool and
protected from moisture. For easy access with a syringe, our derivatization reagents are supplied in vials with crimp caps. Vials
with pierced sealing disks have limited stability and should be used soon.
www.mn-net.com
283
Apart from a few exceptions, methylation is the most common alkylation type.
Methylation reagents
H 3C
N,N-dimethylformamide dimethylacetal
DMF-DMA M 119.17g/mol, Bp 106107C
H 3C
H 3C
Trimethylsulfonium hydroxide
TMSH (0.2mol/L in methanol) M 94.06g/mol
H 3C
CH3
O
CH3
CH3
OH
Reagents for GC
For GC separation of FAMEs from natural butter fat after derivatization with TMSH see Appl. 201680 at www.
mn-net.com
Methylation with DMF-DMA, under formation of N-dimethyl-aminomethylene amino acid methyl esters, is
applicable for fatty acids, primary amines and (partially)
amino acids [Thenot et al., Anal. Letters 5 (1972) 217223, 519
529]. Since DMF-DMA is a poor solvent, it is essential to
use a mixture of DMF-DMA with pyridine, THF, acetone
(barbiturates) or another solvent.
Procedure:
Add 1mL of a mixture of DMF-DMA and pyridine (1:1) to
150mg fatty acids. The sample can be injected as soon
as a clear solution has formed. It is recommended, however, to heat the solution to 60100C for 1015min.
Ordering information
Substance
DMF-DMA*
TMSH*
10 x 1mL
701520.101
20 x 1mL
701430.201
701520.201
Packing unit
1 x 10mL
701430.110
701520.110
5 x 10mL
701520.510
* These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.
Due to their purpose, derivatization reagents are very reactive substances. For this reason, they should be stored cool and
protected from moisture. For easy access with a syringe, our derivatization reagents are supplied in vials with crimp caps. Vials
with pierced sealing disks have limited stability and should be used soon.
284
info@mn-net.com
Reactivity of silylation reagents (acc. to M. Donike): TMS amides (e.g., BSA, MSTFA) > TMS amine = TSIM >
Enol-O-TMS ether > S-TMS ether > O-TMS ether > TMS-O-TMS
Stability of the TMS derivatives: O-TMS ether > S-TMS ether > Enol-O-TMS ether > TMS amine > TMS amide
BSA: R = CH3
Si(CH3)3
Si(CH3)3
Strong silylation reagent, creating very stable TMS derivatives of a multitude of compounds, e.g., alcohols,
amines, carboxylic acids, phenols, steroids, biogenic amines and alkaloids; not recommended for use with
carbohydrates or very low molecular weight compounds; good solvent for polar compounds, but frequently
used in combination with a solvent (pyridine, DMF etc.) or with other silylation reagents. Dissolved in DMF,
BSA is the prime derivatization reagent for phenols.
N,O-bis-trimethylsilyl-trifluoroacetamide
BSTFA: R = CF3
Powerful trimethylsilyl donor with approx. the same donor strength as the nonfluorinated analog BSA
Advantage of BSTFA over BSA: greater volatility of its reaction products, particularly useful for GC analysis
of low boiling TMS amino acids
BSTFA is nonpolar (less polar than MSTFA) and can be mixed with acetonitrile for improved solubility. For
the silylation of fatty acid amides, hindered hydroxyl groups and other difficult to silylize compounds, e.g.,
secondary alcohols and amines, we recommend BSTFA + 1% trimethylchlorosilane (TMCS), available under
the designation SILYL-991.
Procedure:
Add 0.5mL of the silylation reagent to 110mg sample;
if necessary, add some solvent (normally pyridine or DMF
[dimethylformamide]). Heat to 6080C for 20min to
increase the reaction rate. 12 drops of TMCS (trimethyl
chlorosilane) or TSIM will also speed up the reaction.
Reagents for GC
N,O-bis-trimethylsilyl-acetamide
RC
Procedure:
BSA alone silylates all sterically unhindered hydroxyl
groups of the steroid skeleton; addition of TMCS will enable reaction of moderately hindered OH groups (reaction
time 36h at 60C). After addition of TSIM even strongly
hindered hydroxyl groups will react (reaction time 624h
at 60C).
Ordering information
Substance
BSA*
BSTFA*
SILYL-991*
(BSTFA TMCS (99:1)
20 x 1mL
701220.201
701490.201
1 x 10mL
701210.110
701220.110
Packing unit
5 x 10mL
701210.510
701220.510
1 x 50mL
1 x 100mL
701490.150
701490.1100
701210.150
* These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.
www.mn-net.com
285
Reagents for GC
N-methyl-N-trimethylsilyl-trifluoroacetamide
MSTFA:
RCO
CH3
Si (CH3)2 R
R = CF3, R = CH3
Very strong TMS donor which does not cause noticeable FID fouling even during long-time measuring
series. The addition of protic solvents in submolar quantities, e.g., TFA for extremely polar compounds
(hydrochlorides) or pyridine for carbohydrates), can improve the already good dissolving power of MSTFA.
Recommended applications: carboxylic acids, hydroxy and ketocarboxylic acids, amino acids, amines, alcohols, polyalcohols, sugars, mercaptans and similar compounds with active hydrogen atoms. Even amine
hydrochlorides can be silylated directly.
Advantages: complete conversion with high reaction rates, even without a catalyst (12% TMCS or TSIM); the
by-product of the reaction (N-methyltrifluoroacetamide) shows a high volatility and a short retention time
N-methyl-N-trimethylsilyl-heptafluorobutyramide
Recommended applications: carboxylic acids, alcohols, phenols, primary and secondary amines and amino
acids; either applied alone or in combination with a catalyst (TMCS, TSIM) or another silylation reagent
with or without solvent; the by-product N-methylheptafluorobutyric amide has a lower retention time than
the silylating reagent; especially useful for flame ionization detection due to the large ratio of fluorine to
silicon of 7:1, since degradation of the surplus MSHFBA does not produce SiO2 but volatile, non-corrosive
silicon compounds
N-methyl-N-tert-butyldimethylsilyl-trifluoroacetamide
MBDSTFA
R = CF3, R = C4H9
(MTB-TFA):
Silylation reagent that donates a tert-butyldimethylsilyl group (TBDMS) for derivatizing active hydrogen
atoms in hydroxyl, carboxyl and thiol groups as well as primary and secondary amines; fast reactions (typically 520min) with high yields (> 96%); by-products are neutral volatiles
TBDMS ethers are 104 times more stable than the corresponding TMS ethers
Due to the large protecting group, chromatographic retention times are longer. This may have a beneficial
impact on some separations. The high concentration of M+-57 ions is an interesting topic for GC/MS.
MSTFA MN Appl. No. 213111 MSHFBA MN Appl. No. 213112 MBDSTFA MN Appl. No. 213113
Ordering information
10 x 1mL
MSHFBA*
MSTFA*
MBDSTFA*
701440.101
Packing unit
5 x 10mL
1 x 100mL
20 x 1mL
1 x 10mL
701260.201
701260.110
701260.510
701260.1100
701270.201
701270.110
701270.510
701270.1100
6 x 50mL
6 x 100mL
12 x 100mL
701260.6100
701270.650
701270.6100 701270.12100
701440.201
* These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.
286
info@mn-net.com
Dimethyldichlorosilane
DMCS: (CH3)2SiCl2
Used to form dimethylsilyl (DMS) derivatives; DMS derivatives are much more susceptible to hydrolysis than
TMS derivatives, it is therefore vital to have strictly anhydrous conditions during the conversion.
Hexamethyldisilazane
Weak TMS donor; used as a sole reagent, it is slow and not very effective.
Aprotic solvents like acetonitrile, pyridine, dimethylformamide, carbon disulfide and dimethylacetamide
recommend themselves for use with HMDS.
Trimethylchlorosilane
TMCS: (CH3)3SiCl
Reagents for GC
With catalytic quantities, e.g., 1% of, or as a mixture with TMCS (2:1, v/v; SILYL-21 and SILYL-2110) it is
perfectly suited for a quick and quantitative trimethylsilylation of organic compounds.
TSIM:
CH3
H3C
Si
CH3
It is remarkable that TSIM reacts quickly and smooth with hydroxyl (even tert. OH) and carboxyl groups,
but not with amines. Hence it is especially suited for multiple derivatizations, when compounds with various functional groups are to be derivatized in different ways (e.g., -O-TMS, -N-HFB derivatives of catecholamines).
Recommended applications:
alcohols, phenols, organic acids, steroids, hormones, glycols, nucleotides, narcotics
Ordering information
Substance
DMCS*
HMDS*
TMCS*
TSIM
20 x 1mL
701280.201 **
701310.201
1 x 10mL
Packing unit
5 x 10mL
701240.510
701310.110
701310.510
6 x 50mL
701230.650 **
701240.650 **
701280.650 **
* These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.
Due to their purpose, derivatization reagents are very reactive substances. For this reason they should be stored cool and
protected from moisture. For easy access with a syringe, our derivatization reagents are supplied in vials with crimp caps (** in
vials with screw caps). Vials with pierced sealing disks have limited stability and should be used soon.
www.mn-net.com
287
Composition
BSA - HMDS - TSIM (2:7:1)
TSIM - pyridine (11:39)
HMDS - TMCS (2:1)
HMDS - TMCS - pyridine (2:1:10)
BSTFA - TMCS (99:1)
20 x 1mL
701450.201
701460.201
701470.201
701480.201
701490.201
1 x 10mL
701450.110
5 x 10mL
701450.510
1 x 50mL
1 x 100mL
701490.150
701490.1100
* These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.
Due to their purpose, derivatization reagents are very reactive substances. For this reason, they should be stored cool and
protected from moisture. For easy access with a syringe, our derivatization reagents are supplied in vials with crimp caps. Vials
with pierced sealing disks have limited stability and should be used soon.
Reagents for GC
Procedure:
Carefully add SILYL-21 or SILYL-2110 to 110mg of the
sample. Precipitated ammonium chloride does not interfere. If the sample should not dissolve within 5min, heat
to 7585C. If no mutarotation is to be expected, you may
dissolve the sugar in warm pyridine first and then add the
silylation reagent. In some cases it may be advantageous
to use a different solvent instead of pyridine. For derivatization of 3-ketosteroids we recommend to use DMF
(dimethylformamide).
Recommended applications: sugars, glycols, sterically unhindered alcohols, carboxylic acids, acids
in urine, hydroxy fatty acids, nucleotides, steroids,
vitamin D, xanthone derivatives
288
info@mn-net.com
Ordering information
Designation
Composition
Pack of
REF
722306
1mL
722307
1mL
722310
1mL
722316
1mL
722317
1mL
722320
Pack of
REF
(in mg/mL) n-decane (~2.8), n-undecane (~2.9), n-octanol (~3.6), 2,6dimethylphenol (~3.2), 2,6-dimethylaniline (~3.2), methyl decanoate (~4.2),
dicyclohexylamine (~3.1), methyl undecanoate (~4.2), methyl dodecanoate
(~4.1)
0.1% each of undecane, dodecane, octanol, dimethylaniline, decylamine,
methyl decanoate, methyl undecanoate, henicosane, docosane, tricosane
(chromatograms see page 240)
0.1% each of FAMEs C4, C6, C8, C10, C12, C14, C16, C18, C18:1 cis,
C18:1 trans, C18:2, C18:3, C20, C22, C22:1, C24 (chromatogram see page
262)
1% phenylethanol in CH2Cl2
1mL
722321
methylbutyrolactone
phenylethylamine (TFA)
phenylethanol (TFA)
1mL
1mL
1mL
722322
722323
722319
Reagents for GC
1mL
These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see
MSDS.
www.mn-net.com
289
Column:
Injection:
Carrier gas:
Temperature:
Detector:
6
4
3
4
Reagents for GC
10
min
MN Appl. No. 250020
Column:
OPTIMA 5, 1.0m film, 25m x 0.32mm ID
Injection:
1L, split 1: 40, 300C
Carrier gas:
0.6bar H2
Temperature:
150C 300C (8min), 10C/min
Detector:
FID 300C
Peaks:
1. TMS capric acid (C10)
2. Hexadecane (C16)
3. TMS myristic acid (C14)
4. Eicosane (C20)
5. TMS stearic acid (C18)
6. Tetracosane (C24)
7. TMS behenic aicd (C22)
8. Octacosane (C28)
1
2
3
10
15
min
Column:
OPTIMA 5, 1.0m film, 50m x 0.25mm ID
Injection:
1L, split 1: 40, 280C
Carrier gas:
1.5bar H2
Temperature:
80C 280C (10min), 8C/min
Detector:
FID 280C
Peaks:
1. n-Decane
2. 1-Octanol
3. n-Undecane
4. 2,6-Dimethylphenol
5. 2,6-Dimethylaniline
6. Methyl decanoate
7. Methyl undecanoate
8. Dicyclohexylamine
9. Methyl dodecanoate
6
1
7 9
3
2 4 5
8
min
290
10
15
20 min
MN Appl. No. 211250
info@mn-net.com
Pack of
REF
1mL
722311
1mL
722371
11 x 1mL
722312
1mL
722314
1mL
722331
1mL
722372
These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see
MSDS.
Column:
OPTIMA 5, 0.25m film, 30m x 0.32mm ID
Sample:
PAH test mixture according to EPA (20g/mL each in toluene)
Injection:
1.0L, split 1: 15
Carrier gas:
H2, 70kPa
Temperature:
100C, 7C/min 300C
Detector:
FID 300C
Peaks:
1
2 3
5 6
4
1. Naphthalene
2. Acenaphthylene
3. Acenaphthene
4. Fluorene
5. Phenanthrene
6. Anthracene
7. Fluoranthene
8. Pyrene
9. Benz[a]anthracene
10. Chrysene
11. Benzo[b]fluoranthene
12. Benzo[k]fluoranthene
13. Benzo[a]pyrene
14. Indeno[1,2,3-cd]pyrene
15. Dibenz[ah]anthracene
16. Benzo[ghi]perylene
www.mn-net.com
10
7 8
Reagents for GC
Composition
10
11 12
13
14
15
16
20
30 min
291
Column:
OPTIMA 5, 0.25m film, 25m x 0.,32mm ID
Injection:
2L, split 1: 10
Carrier gas:
0.6bar H2
Temperature:
80C 180C 300C, 4C/min
Detector:
FID 300C
Peaks:
1. Fluoranthene
2. Benzo[b]fluoranthene
3. Benzo[k]fluoranthene
4. Benzo[a]pyrene
5. Indeno[1,2,3-cd]pyrene
6. Benzo[ghi]perylene
1
Column:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
1. Dichloromethane
2. Trichloromethane
3. 1,1,1-Trichlorethane
4. Tetrachloromethane
5. Trichloroethene
6. Bromodichloromethane
7. Dibromochloromethane
8. Tetrachloroethene
9. Tribromomethane
Reagents for GC
3
3
2
4
5
6
1
20
min
10
15
Column:
Injection:
Carrier gas:
Temperature:
Detector:
Peaks:
M = methanol
1. Benzene
2. Toluene
3. p-Xylene
4. m-Xylene
5. Ethylbenzene
6. o-Xylene
min
M
1
34
292
10
56
20 min
info@mn-net.com
Vespel ferrules come in three types: pure Vespel, Vespel with 15%
graphite and Vespel with 40% graphite. All versions are temperature-stable up to 400C and reusable.
PTFE ferrules can only be used up to 250C. They are not reusable and not recommended for temperature programming. However, they show the best chemical inertness of all ferrules.
1/16" ferrules
450C
no bore
0.4mm
0.5mm
0.8mm
1.0mm
1.2mm
1/16"
708336
708309
708308
708301
708302
708303
706155
0.4mm
0.5mm
0.8mm
708338
708339
708340
0.4mm
0.5mm
0.8mm
708353
708354
708355
no bore
0.4mm
0.5mm
0.8mm
1/16"
1/8"
708341
708342
708343
708333
708158
708156
no bore
0.4mm
0.5mm
1/16"
1/8"
6.0mm
1/4"
708344
708345
708346
plain
400C
Vespel
+ 15% graphite
400C
706187
706167
706180
706160
706190
706168
706249
706240
+ 40% graphite
400C
706246
706247
706248
PTFE
250C
706177
706170
1/8" ferrules
1/4" ferrules
708348
706157
6mm ferrules
no bore
6.0mm
706188
706266
706268
706183
706181
706191
706169
706185
706186
706182
706178
Accessories for GC
max. temperature
Graphite
706171
706199
706164
706196
706192
706252
706176
706172
706259
If you are in doubt about the correct size or REF please send us an old, used ferrule as a sample.
www.mn-net.com
293
Accessories for GC
nut
fitting
capillary 2
one-piece polyimide
adapters
fitting
standard ferrule
liner
capillary 2
ferrule
nut
liner
nut
valve body
FSR:
liner
ferrule
slot
rotor
0.25mm orifice
294
info@mn-net.com
Description
Pack of
REF
for capillary OD
< 0.25mm
0.250.4mm
0.40.5mm
< 0.4mm
0.40.5mm
0.60.8mm
5
5
5
5
5
5
724405
724243
724244
724406
724407
724408
FSR.25-5
FSR.4-5
FSR.5-5
FS1R.5-5
FS1R.8-5
1/32"
1/32"
1/32"
1/16"
1/16"
< 0.25mm
0.250.4mm
0.40.5mm
< 0.5mm
0.50.8mm
5
5
5
5
5
724409
724410
724411
724335
724334
FSL.25-5
FSL.4-5
FSL.5-5
FS1L.5-5
FS1L.8-5
1/32"
1/32"
1/32"
1/16"
1/16"
< 0.25mm
0.250.4mm
0.40.5mm
< 0.5mm
0.50.8mm
5
5
5
5
5
724412
724413
724414
724415
724416
ZCN1
1/16"
counterbored
724417
Replacement liners
Accessories for GC
FS.25-5
FS.4-5
FS.5-5
FS1.4-5
FS1.5-5
FS1.8-5
For standard Vespel ferrules as well as standard nuts please have a look at the Valco program, which is available on request.
Unions, Tees and crosses for fused silica adapters (without ferrules, but incl. standard nuts)
ZU.5TJ
ZU1TJ
1/32" 1/32"
1/16" 1/16"
ZX.5J
ZX1CJ
1/32"
1/16"
ZT.5J
ZT1CJ
1/32"
1/16"
Tee
Tee, capillary bore
cross
cross, capillary bore
1
1
1
1
724418
724333
724421
724336
1
1
724422
724337
1
1
724423
724424
In case of a broken tubing in a through-bore union, remove the nut and the intact tubing on the opposite site
of the broken one. Clear the fitting by pushing a fine
wire or capillary drill through the center.
For other fittings and valves for GC please ask for our VICI / Valco program.
www.mn-net.com
295
Glass connectors for fused silica capillary columns from 0.2 to 0.53mm ID
manufactured from deactivated glass with slightly tapered inner diameter; used to join two fused silica
capillaries of equal or different diameters. Advantages compared to stainless steel fittings are easy connection without tools, optical control during connection, negligible heat capacity and no dead volume.
PTFE shrink tube
also applicable for capillary connection. The minimum ID of the expanded tubing is 1.17mm, the maximum ID of the shrunk tube is 0.40mm. Shrinking occurs above 310C. Connections with PTFE shrink tube
are applicable up to 200C only. They should never be used above 250C.
Ordering information
Accessories for GC
Description
Pack of
REF
Specification
0.4mm bore
0.5mm bore
0.8mm bore
10 ferrules
10 ferrules
10 ferrules
708337
708318
708319
1
linear
linear
Y splitter
5 connectors
10 connectors
1 connector
707971
707972
707973
1m
708305
Septa for GC
Designation
Material
Thickness
Hardness
max. Temp.
1
2
3 2
3
beige silicone
transparent silicone
4mm
60shore A
3mm
60shore A
320C *
3mm
45shore A
250C
* If used at considerably higher temperatures and working without septum purge interfering peaks can occur due to
thermal decomposition of the material.
296
info@mn-net.com
9mm
N9
702609
702619
702602
10mm
N 10
702610
702620
702625
Outer diameter
11mm
12mm
N 11
N 12
702611
702621
702604
702626
702612
702622
702605
702627
13mm
N 13
702613
702623
702606
702628
17mm
N 17
702632
706141
Accessories for GC
Diamond file:
a useful tool for cutting capillaries and smoothing ends of capillaries. Square capillary ends are especially
important for butt connections (e.g., in Valco unions).
0 1 2 3 4 5 6 7 8 9 10
Diamond file
Ordering information
Description
Glass wool
Specification
for cutting capillaries and straightening capillary ends
magnification 8x
www.mn-net.com
Pack of
REF
1
1
708300
706296
50g
25g
25g
1
1 reel
706201
718002
718587
706117
706512
297
Appendices
298
Amino-modified silica
CHROMABOND Flash RS columns
CHROMABOND SPE phase
layers for HPTLC
NUCLEODUR NH2 columns for HPLC
NUCLEOSIL columns for HPLC
NUCLEOSIL packings for HPLC
POLYGOPREP packings for preparative HPLC
POLYGOSIL packings for HPLC
-Aminopolycaprolactam seePolyamide
Analytical columns for HPLC
NUCLEODUR
NUCLEOSHELL Core-shell silica phases
NUCLEOSIL
other manufacturers: LiChrospher
special separations
Anion analysis by HPLC
Anion exchangers
for HPLC
NUCLEOGEL Anion I polymer-based columns
NUCLEOGEL SAX polymer-based columns
NUCLEOGEN DEAE silica-based columns
NUCLEOSIL Anion II silica-based columns
NUCLEOSIL PEI silica-based columns
NUCLEOSIL SB silica-based columns
NUCLEOSIL SB silica-based packing
for SPE
CHROMABOND HR-XA polymer phase
CHROMABOND HR-XAW polymer phase
CHROMABOND PS-OH polymer phase
CHROMABOND SB silica phase
for TLC
cellulose-based layers
resin-based IONEX layers
Application database seewww.mn-net.com/apps
Application guides
GC
reversed phase HPLC
Aqueous eluents in reversed phase HPLC
NUCLEODUR PolarTec
NUCLEODUR Pyramid
NUCLEOSIL Nautilus
Aromatic hydrocarbons seeBTX, PAH and PCB
ASTM D 1319-58T FIA procedure
ASTM D 6751 biodiesel
Autoselectivity, OPTIMA columns
AVICEL cellulose layers for TLC
61
31
222
140
163
200
204
202
116
148
157
166
167
171
171
181
178
171
180
165
201
17
19
22
37
226
228
266
143
124
122
157
204
272
241
225
B
Base deactivated silica RP phases
116
Basic principles
GC
236
SPE
2
TLC
208
BIG-BOX, CHROMAFIL syringe filters
67
BIGpack, CHROMABOND SPE columns
1546
Bioanalysis
HPLC columns
167
nucleic acid and protein purification also ask forour
catalog Bioanalysis
Biodiesel, GC capillary columns for analysis of
methanol, FAMEs and glycerol/triglycerides
272
Biotage, CHROMABOND Flash cartridges
62
info@mn-net.com
103
175
177
285
285
292
291
192
www.mn-net.com
Appendices
CA seeCellulose acetate
Capillary accessories
for GC
293
for HPLC
196
Capillary columns for GC
OPTIMA high performance columns
summary
238
autoselectivity
241, 249250
fast GC
267
high temperature GC
275
low bleed
243, 246, 252, 261, 263
thick films
242, 245
ultra-low bleed
244, 247248, 254, 256
PERMABOND columns
264
special separations
summary
266
amines
276277
biodiesel, analysis of methanol, FAMEs
and glycerol/triglycerides
272
diethylene glycol analysis
279
enantiomer analysis
268271
environmental analysis
278
petrochemical analysis
277
silane analysis
279
triglyceride analysis
274
Capillary connectors for GC
296
Capillary electrophoresis
untreated fused silica capillaries
280
Capillary fittings for GC
294
Capillary tubing cutter
for HPLC stainless steel capillary tubing
196
Capillary tubing cutters
diamond file for GC capillaries
297
for PEEK HPLC capillaries
197
for stainless steel HPLC capillaries
196
Capillary tubing for HPLC
PEEK
197
stainless steel
196
Capillary unions for GC
296297
Capillary unions for HPLC
196
Carbohydrate analyses, HPLC columns
185187
Carlo Erba seeFisons
Cartridges CHROMAFIX for SPE
6
Cartridge system ChromCart for HPLC
192
Cation exchangers
for HPLC
NUCLEOGEL SCX polymer-based columns
181
NUCLEOSIL SA silica-based columns
164
NUCLEOSIL SA silica packing
200
for SPE
CHROMABOND HR-XC polymer phase
16
CHROMABOND HR-XCW polymer phase
18
CHROMABOND PCA, PSA silica phases
35
CHROMABOND PS-H+, PS-Ag+, PS-Ba2+
polymer phases
22
CHROMABOND SA silica phase
36
for TLC
resin-based IONEX layers
228
Celite kieselguhr
205
Cellulose
adsorbents for column liquid chromatography
206
adsorbents for TLC
234
layers for TLC
225
Cellulose acetate syringe filters CHROMAFIL
71
Cellulose, acetylated, layers for TLC
226
Cellulose aluminium oxide, mixed layers for TLC
229
Cellulose AVICEL, layers for TLC
225
Cellulose-based phase for HPLC enantiomer separation 174
Cellulose mixed esters
filter plates CHROMAFIL MULTI 96
76
syringe filters CHROMAFIL
70
Cellulose silica mixed layers for TLC
229
Chamber saturation in TLC
209
Chemical compatibility of CHROMAFIL filters
74
Chemically bonded phases for GC seeOPTIMA and
PERMABOND
Chiral phases for enantiomer separation
GC capillary columns
268271
HPLC columns
172177
CHIRALPLATE for TLC enantiomer separation
226
CHROMABOND phases for SPE
1449
summary
89
Flash RS columns
61
HR-Xpert concept
10
vacuum manifolds and accessories
5052
CHROMAFIL
filtration cartridges
75
MULTI 96 filter plates
76
syringe filters (membrane filters)
6674
integrated glass fiber prefilters
6768
sterile filters
71
CHROMAFIX cartridges for SPE
6
Chromatography papers
229
ChromCart cartridge system for HPLC
192
Closures for vials
89, 94, 101
crimp closures
85, 89, 100
screw closures
79, 81, 83, 9093, 95, 103
snap closures
87, 94
stoppers
89, 94, 101
Column accessories for HPLC
196198
Column Protection System
guard column system for analytical HPLC
190
Columns for GC seeCapillary columns for GC
Columns for HPLC seeAnalytical columns and Preparative
HPLC, NUCLEODUR
Columns for SPE, CHROMABOND
1449
Column systems and columns accessories for HPLC 189195
Compatibility of CHROMAFIL syringe filters
74
299
Appendices
Concentrating zone
layers for TLC
216
sample application in TLC
208
Conic vials seeVials
Connectors for GC capillaries
glass connectors, PTFE shrinking tube
296
Valco fused silica adapters
294295
Connectors for SPE vacuum manifolds
51
Containers for vials
8889, 100
Core-shell technology
phases for analytical HPLC
144
Crimp closures
aluminium
78, 85, 89, 100
magnetic
8485, 101
Crimping tools
86, 89, 98
Crimp neck vials and closures
8486, 89, 98101
Crosses (Valco) for fused silica adapters for GC
295
Crosslinks, enrichment, CHROMABOND phase for SPE
40
Cutter for stainless steel capillaries in HPLC
196
Cyano-modified silica
CHROMABOND Flash RS columns
62
CHROMABOND SPE phase
30
layers for HPTLC
221
NUCLEODUR columns for HPLC
138
NUCLEOSIL columns for HPLC
164
NUCLEOSIL packings for HPLC
200
POLYGOPREP packings for preparative HPLC
203
POLYGOSIL packings for HPLC
202
Cyclodextrins as chiral stationary phases
HYDRODEX for GC
270
LIPODEX for GC
268
NUCLEODEX for HPLC
172
D
Deactivated capillaries for GC
280
DEAE anion exchangers
silica-based HPLC columns NUCLEOGEN DEAE
178
Derivatization
method development kits
282
reagents and procedures for GC
283288
selection guide for important functional groups
282
Detection of substances in TLC
210
Detection reagents for TLC
233
Developing a thin layer chromatogram
209
Developing chambers for TLC
209, 233
Diamino-modified silica phase for QuEChERS method
48
Diamond file for GC
297
Diethylaminoethyl seeDEAE
Diethylene glycol analysis, capillary column for GC
279
Dimethyldichlorosilane (DMCS)
287
N,N-dimethylformamide dimethylacetal (DMF-DMA)
284
Dimethyl-modified silica
layers for TLC
220
NUCLEOSIL columns for HPLC
162
NUCLEOSIL packings for HPLC
200
SPE phase CHROMABOND C2
27
DIN 38407 part 5 halogenated hydrocarbons
291
DIN 38407 part 7 PAH in drinking water
228
DIN 38409 - H22 AOX from waters
41
DIN 51527 PCB from oil
46
DIN 51791 FIA procedure
204
DIN EN 14214 biodiesel
272
DIN H-53 hydrocarbons from water
43
300
Diol-modified silica
CHROMABOND Flash RS columns
62
CHROMABOND SPE phase
30
layers for HPTLC
223
NUCLEOSIL columns for HPLC
162
NUCLEOSIL packings for HPLC
200
Disaccharide separation, HPLC columns
185187
Dispersive SPE, QuEChERS method
48
Disposable cartridges CHROMAFIX for SPE
6
Disposable columns CHROMABOND for SPE
6
Disposable filters CHROMAFIL
66
DMCS (dimethyldichlorosilane)
287
DMF-DMA (N,N-dimethylformamide dimethylacetal)
284
Drug analysis
HPLC enantiomer separation
173
HPLC of basic drugs
116
SPE of acidic, neutral and basic drugs from urine
or plasma
38
SPE of tetracyclines from biological samples
40
SPE of THC and acidic analytes from biological fluids
39
Drying of organic samples, CHROMAFIX cartridges
47
DURASIL glass plates for TLC and HPTLC
215, 218
Dye analysis, TLC chromatograms
217, 224
E
Easy, polar adsorbent resin for SPE
EC column system for analytical HPLC
Electrophoresis, untreated fused silica capillaries
Eluent filters, stainless steel
Empty CHROMABOND columns for SPE
Enantiomer separation
GC capillary columns
HPLC columns
TLC plates
Environmental analysis
GC capillary columns
HPLC columns
SPE method development
SPE polymer phases
SPE special phases for AOX and hydrocarbons
SPE special phases for PAH
SPE special phases for PCB
test mixtures for GC
EPA 610
GC chromatogram
EPA 610, PAH standard
EPA 8140/8141 organophosphorus pesticides
GC chromatograms
EPA screw neck vials
Evaluation of a thin layer chromatogram
Exclusion chromatography
also seeGPC
20
189191
280
196
52
268271
172177
226
278
168171
7
1421
41, 43
42, 44
4446
291
254
170
244, 250
102
209
159
F
FAMEs
GC analysis in biodiesel
GC chromatograms
FAME test mixture for GC
Fast GC
Fast HPLC
Fatty acid methyl esters seeFAME
273
258259, 262263, 273
289
267
111, 144
info@mn-net.com
G
GC accessories seeAccessories for GC
GC columns seeCapillary columns for GC
GC/MS and low bleed columns 243244, 246248, 252, 254
Gel permeation chromatography (GPC), columns
188
Glass bottles seeVials
Glass columns CHROMABOND for SPE
6
Glass connectors for fused silica capillaries
296
Glass fiber
filtration cartridges CHROMAFIL
75
prefilters, CHROMAFIL Combi Filters
6768
syringe filters CHROMAFIL
73
Glass fiber wadding silanized, very fine fibers
297
Glass plates for TLC
211
Glass wool, long fibers, DMCS treated
297
Glycerol and glycerides in biodiesel
GC capillary columns for analysis
272
GPC columns
188
Graphite and graphite/Vespel ferrules
293
Gravity, high density NUCLEODUR RP phases
116
Grob test mixture
289
chromatograms
260, 290
195198
190
191
195
280
197
H
Halogenated hydrocarbons
GC capillary column
278
GC chromatograms
278, 292
test mixtures for GC
291
Heptafluorobutyric acid anhydride (HFBA)
283
Herbicide analysis seePesticide analysis
Hexamethyldisilazane (HMDS)
287
HFBA (heptafluorobutyric acid anhydride)
283
High density RP phases for HPLC
116119, 130132
NUCLEODUR
148149
NUCLEOSHELL
NUCLEOSIL
157158, 160161
High performance capillary columns for GC
seeOPTIMA capillary columns
High performance plates for TLC seeHPTLC
High purity silica for HPLC seeNUCLEODUR
High temperature GC
OPTIMA capillary columns
275
High temperature septa for GC
297
High-throughput screening
with CHROMABOND Flash
6067
High-throughput SPE
53
HILIC (Hydrophilic Interaction Chromatography)
NUCLEODUR phase
136
NUCLEOSHELL phase
152
HMDS (hexamethyldisilazane)
287
HPLC columns seeAnalytical columns
and Preparative HPLC, NUCLEODUR
HPLC column systems
189195
HPTLC plates
216223, 226, 228
HR-P adsorbent resin for SPE
21
HR-X, spherical hydrophobic adsorbent resin for SPE
14
HR-XA, strong mixed-mode anion exchanger for SPE
17
HR-XAW, weak mixed-mode anion exchanger for SPE
19
HR-XC, strong mixed-mode cation exchanger for SPE
16
HR-XCW, weak mixed-mode cation exchanger for SPE
18
HR-Xpert concept
10
Hydrocarbon analysis
FIA procedure, silica
204
SPE phase CHROMABOND Na2SO4/Florisil
43
also seeBTX, Halogenated hydrocarbons and PAH
HYDRODEX columns for GC enantiomer separation
270
Hydrophilic RP phases for HPLC
NUCLEODUR HILIC
136
NUCLEODUR PolarTec
124125
NUCLEODUR Pyramid
122
NUCLEOSIL Nautilus
158
Hyflo Super-Cel kieselguhr
205
Appendices
Ferrules
for HPLC columns
196
graphite, Vespel, PTFE for GC
293
Graphseal for GC
296
PEEK
197
FFAP (Free Fatty Acid Phase)
262263, 265
FIA procedure, silica
204
Fibrous cellulose seeCellulose
File (diamond) for GC
297
Film thickness of GC capillary columns
237
Filter-Cel kieselguhr
205
Filter plates CHROMAFIL MULTI 96
76
Filters
CHROMAFIL syringe filters
66
stainless steel, for HPLC eluents
196
also ask forour catalog Filtration
Filtration cartridges CHROMAFIL
75
Fisons GC instruments, accessories
ferrules
293
Fittings
PEEK for HPLC
197
stainless steel for HPLC
196
Valco for capillary GC
294
Flash chromatography
adsorbents
64
glass columns and accessories
59
solutions for specific instruments
61
FlashMaster systems, CHROMABOND Flash cartridges 61
Florisil magnesium silicate
for column chromatography
206
for SPE
34, 43
Fluorescence indicator adsorption procedure, silica
204
Fluorescent indicators for TLC
234
Food analysis, HPLC columns
185187
Fused silica adapters for capillary GC
294
Fused silica capillaries seeCapillary columns
I
Immobilized phases for GC seeOPTIMA and PERMABOND
Impregnated cellulose PEI anion exchange layers for TLC 226
www.mn-net.com
301
Appendices
Kieselguhr
adsorbents for column chromatography
phase for liquid-liquid extraction
Knife file for cutting stainless steel capillary tubing
205
5657
196
L
LC accessories
for stainless steel columns
189196, 196200
made from PEEK
197
Length of GC capillary columns
237
Lens with scale, 8fold magnification
297
LiChrospher ChromCart cartridges
166
Ligand exchange for enantiomer separation
CHIRALPLATE for TLC
226
HPLC columns NUCLEOSIL CHIRAL-1
176
LIPODEX columns for GC enantiomer separation
268
Liquid-liquid extraction, CHROMABOND XTR
5657
Low bleed capillary columns for GC
243, 246, 252, 254, 261, 263
also seeUltra-low bleed columns
Low pressure column chromatography, adsorbents
204
M
Magnesium silicate seeFlorisil
Magnetic closures for vials
crimp closures
8485, 101
screw closures
95
Magnifying lens with scale, 8fold
297
MBDSTFA (N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide)
286
MBHFBA (N-methyl-bis(heptafluorobutyramide))
283
MBTFA (N-methyl-bis(trifluoroacetamide))
283
Membrane filters CHROMAFIL
74
Methanol, GC capillary column
for analysis in biodiesel
272
Method development kits
for derivatization in GC
282
for HPLC enantiomer separation (NUCLEODEX)
173
for HPTLC
223
for SPE
7
302
N
Nano-ADAMANT glass plates for HPTLC
217
Nano silica layers for HPTLC
216223, 226, 228
Nautilus, hydrophilic NUCLEOSIL RP phase
158
Needles for SPE vacuum manifolds
51
Nucleic acid analysis
HPLC columns
178
also ask forour catalog Bioanalysis
NUCLEOCEL for HPLC enantiomer separation
174
NUCLEODEX for HPLC enantiomer separation
172
NUCLEODUR high purity silica for HPLC
110
phase summary
112115
preparative packings (bulk)
198
NUCLEOGEL columns
for anion analysis by HPLC
171
for GPC
188
for ion exchange HPLC of biological macromolecules 181
for RP separation of biological macromolecules
184
for sugar analysis by HPLC
186
NUCLEOGEN DEAE for separation of nucleic acids
178
NUCLEOSHELL core-shell silica
phases
148
technology
144
NUCLEOSIL standard silica for HPLC
154
columns, phase summary
155
packings (bulk)
199
Nylon 6 seePolyamide
O
Oligosaccharide separation, HPLC columns
Optical purity seeEnantiomer separation
info@mn-net.com
186187
238
267
275
252, 254
242,
254,
244,
126,
276
272
274
245
256
250
150
www.mn-net.com
Appendices
PA seePolyamide
Packings for HPLC
NUCLEODUR spherical silica for preparative HPLC
198
199201
NUCLEOSIL spherical silica
POLYGOPREP irregular silica
203204
201202
POLYGOSIL irregular silica
Packings for low pressure column chromatography 204, 206
PAH analysis
GC chromatograms
248, 254, 292
GC test mixtures
291
HPLC columns
168, 170
HPLC standard according to EPA
170
SPE phases
42, 44
TLC plates
228229
Particle size in HPLC
111, 144
PCB analysis
GC chromatograms
248, 251
GC low bleed columns
244, 247248, 254
SPE phases
4446
PE seePolyethylene
PEEK fittings and capillary tubing for HPLC
197
PEI anion exchangers
HPLC columns NUCLEOGEL SAX, polymer-based
181
180
HPLC columns NUCLEOSIL PEI, silica-based
TLC layers, impregnated cellulose
226
Pentafluorobenzoyl chloride (PFBC)
283
Peptide analysis, HPLC columns
180183
PERMABOND capillary columns for GC
264265
diethylene glycol analysis
279
petrochemical analyses
277
silane analysis
279
volatile halogenated hydrocarbons
272, 275, 278
PES syringe filters CHROMAFIL
71
Pesticide analysis
GC chromatograms
244, 250251, 255256
GC low bleed columns
244, 247248, 254
SPE phases
1421
TLC chromatogram
223
PET seePolyester
Petrochemical analyses, GC capillary column
277
PFBC (pentafluorobenzoyl chloride)
283
PFP (pentafluorophenyl)
hydrophobic NUCLEODUR phase
126
hydrophobic NUCLEOSHELL phase
150
Phase separation, CHROMABOND PTS, PTL
58
Phenol analysis
GC chromatogram
249
SPE phases
1415, 2021
Plasmid analysis
HPLC columns
179
also ask forour catalog Bioanalysis
Plugs for shell vials
94
PMD technique in TLC
209
Polarity mixture for GC capillary columns
289
PolarTec, hydrophilic NUCLEODUR RP phase for HPLC
124
Polyamide
adsorbents for column chromatography
206
adsorbents for TLC
234
layers for TLC
226
SPE phase CHROMABOND PA
34
syringe filters CHROMAFIL
72
Polychlorinated biphenyls seePCB
Polycyclic aromatic hydrocarbons seePAH
Polyester
filtration cartridges CHROMAFIL
75
syringe filters CHROMAFIL
69
with integrated glass fiber prefilter
68
Polyester sheets POLYGRAM for TLC
211
Polyethersulfone syringe filters CHROMAFIL
71
Polyethylene
caps for vials
85, 102
filter plates CHROMAFIL MULTI 96
76
plugs for shell vials
94
snap caps
94
snap ring closures
87
Polyethyleneimine seePEI
POLYGOPREP irregular silica for preparative HPLC
203
POLYGOSIL irregular silica for HPLC
201
POLYGRAM polyester sheets for TLC
211
Polymerically modified silica RP phases
for HPLC
120-121, 157-158, 183
Polymer phases for HPLC seeNUCLEOGEL columns
Polymer phases for SPE
1018
Polypropylene columns and cartridges for SPE
6
Polytetrafluoroethylene seePTFE
Polyvinylidene difluoride seePVDF
Post-chromatographic detection methods in TLC
210
visualization reagents
233
Precolumns (fused silica) for GC
280
Prefilters
CHROMAFIL syringe filters with integrated glass fiber
prefilters
6768
Preparative HPLC
NUCLEODUR C18 HTec with high capacity
130
NUCLEODUR high purity silica phases
116
NUCLEODUR packings (bulk)
198
POLYGOPREP bulk packings
203
special phases for biochemical separations
179, 183
Principles of GC
236
Principles of SPE
2
Principles of TLC
208
Programmed Multiple Development in TLC
209
Protect, hydrophilic NUCLEOSIL RP phase for HPLC
160
Protein analysis by HPLC
180183
Protein phase RESOLVOSIL
for HPLC enantiomer separation
175
PSA
CHROMABOND Diamino phase for SPE (QuEChERS)
48
propylsulfonic acid cation exchanger for SPE
35
303
Q
Quality management
Quartz wool, very fine fibers
QuEChERS method, SPE phase CHROMABOND
Diamino and QuEChERS mixes
5, 143
297
48
Appendices
RC seeRegenerated cellulose
Reaction vials and closures
Ready-to-use layers for TLC
Reagents for
derivatization in GC
post-chromatographic detection in TLC
TLC micro-sets
Regenerated cellulose
filter plates CHROMAFIL MULTI 96
filtration cartridges CHROMAFIL
syringe filters CHROMAFIL
with integrated glass fiber prefilters
Removal of glass wool from tubes, tool
Replacement parts
for EC columns
for stainless steel HPLC columns
for the Column Protection System
for TLC micro-sets
for VarioPrep columns
Resolution in HPLC
RESOLVOSIL for HPLC enantiomer separation
Retention factor in TLC, definition
Retention gaps (fused silica) for GC
Retention in GC, basic terms and definitions
Reversed phase chromatography
HPLC columns for special separations
HPLC columns with NUCLEODUR phases
summary
HPLC columns with NUCLEOSHELL phases
HPLC columns with NUCLEOSIL phases
layers for TLC and HPTLC
SPE phases CHROMABOND
test mixture for HPLC columns
RP seeReversed phase
91
212
282288
233
230232
76
75
69
68
297
189
196198
190
230232
196
111, 144
175
209
281
236
167
112113
148151
155
219223
8
143
S
Sample clarification, syringe filters CHROMAFIL
Sample injection in GC
304
66
236
Sample preparation
derivatization reagents and procedures
282
solid phase extraction (SPE)
8
syringe filters CHROMAFIL
66
Saturation of the vapor atmosphere in a TLC developing
chamber
209
SAX anion exchangers
CHROMABOND HR-XA polymer phase for SPE
17
CHROMABOND SB silica phase for SPE
37
NUCLEOGEL SAX columns for HPLC
181
NUCLEOSIL SB columns for HPLC
165
NUCLEOSIL SB packings for HPLC
201
Screw neck vials
7980, 8283, 90, 9293, 95, 102
SCX cation exchangers
CHROMABOND HR-XC polymer phase for SPE
16
CHROMABOND SA silica phase for SPE
36
NUCLEOGEL SCX columns for HPLC
181
NUCLEOSIL SA columns for HPLC
164
NUCLEOSIL SA packings for HPLC
200
Separation efficiency in HPLC
111, 144
Septa for GC, septum remover
296
Septa for vial closures
80, 82, 85, 90, 101
Serum bottles seeVials
Shell vials and closures
94
Shrinking tube (PTFE) for GC capillaries
296
Silane analysis, PERMABOND columns for GC
279
Silanized glass fiber wadding, very fine fibers
297
Silanized silica, layers for TLC
220
Silanized vials
80, 84
Silarylene phases for GC
247248, 254, 256
Silica
for column chromatography, adsorbents
204
for HPLC
NUCLEODUR high purity silica
bulk packings
198
columns, summary
112115
NUCLEOSIL standard silica
bulk packings
199
columns, summary
155
POLYGOPREP bulk packings
203
POLYGOSIL bulk packings
201
for SPE, phase summary
8
for TLC
adsorbents
234
layers
212
Silica cellulose mixed layers for TLC
229
Silica ion exchange resin layers for TLC
228
Silicone liners for caps
7895
Silicone septa for GC
297
Silylation
method development kit
282
reagents and procedures
285288
Size exclusion chromatography
159
Snap cap vials and closures
94
Snap ring vials and closures
86
Solid phase extraction
high-throughput devices
53
phase summary
89
principles
2
vacuum manifolds and accessories
50
Solvents, GC chromatograms
243, 252
SPE seeSolid Phase Extraction
Spherical polymer phases for SPE
1015
Sphinx, bifunctional NUCLEODUR RP phase
128
info@mn-net.com
327
326
210
234
V
Vacuum manifolds for SPE
50, 55
Valco fused silica adapters and fittings for capillary GC 294
Valco type columns for HPLC
193
VarioPrep columns for preparative HPLC
194
packed with NUCLEODUR high purity silica
119135
Vespel and Vespel/graphite ferrules for GC
293
Vial Kits
82, 86, 88
Vials and closures
containers
8889, 100
crimp neck vials
78, 8485, 89, 98101
screw neck vials
79, 80, 83, 90, 95, 102
snap ring vials
87
special vials
91, 94
storage vials
9294
Visualization of substances in TLC
210
Visualization reagents for TLC
233
Volatile hydrocarbons
GC capillary columns
252, 278
W
Water analysis
SPE of aromatics, pesticides and phenols
1418
SPE of hydrocarbons and AOX
41, 43
SPE of PAH
42
Water removal
from organic samples, CHROMAFIX Dry cartridges
47
WAX (weak anion exchanger)
CHROMABOND HR-XAW polymer phase for SPE
19
WCX (weak cation exchanger)
CHROMABOND HR-XCW polymer phase for SPE
18
CHROMABOND PCA silica phase for SPE
35
96-well format for SPE and filtration
6
Wine analysis
determination of diethylene glycol by GC
279
TLC wine set
232
Appendices
USP specification
of GC phases
of HPLC phases
UV absorption in TLC
UV indicators for TLC
U
Ultra-fast HPLC (UHPLC)
111, 144
Ultra-low bleed capillary columns for GC 244, 247248, 254
Unions
for capillary GC
296297
for HPLC
197
Untreated fused silica capillaries
280
www.mn-net.com
305
Appendices
REF Page
REF Page
REF Page
REF Page
531011 233
701110.110 283
701110.201 283
701110.510 283
701120.101 283
701130.110 283
701130.510 283
701210.110 285
701210.150 285
701210.510 285
701220.110 285
701220.201 285
701220.510 285
701230.650 287
701240.510 287
701240.650 287
701260.110 286
701260.1100 286
701260.201 286
701260.510 286
701260.6100 286
701270.110 286
701270.1100 286
701270.12100 286
701270.201 286
701270.510 286
701270.6100 286
701270.650 286
701280.201 287
701280.650 287
701310.110 287
701310.201 287
701310.510 287
701410.110 283
701410.201 283
701410.510 283
701420.101 283
701420.201 283
701430.110 284
701430.201 284
701440.101 286
701440.201 286
701450.110 288
701450.201 288
701450.510 288
701460.201 288
701470.201 288
701480.201 288
701490.1100 285
701490.1100 288
701490.150 285
701490.150 288
701490.201 285
701490.201 288
701520.101 284
701520.110 284
701520.201 284
701520.510 284
701950 282
701951 282
701952 282
701953 282
702001 85
702002 78
702003 78
702004
79, 92
702005
79, 84
702006 80
702007 80
702008 80
702009 80
702011 83
702012 83
702013 83
702014 85
702015 85
702016 85
702017 94
702018 94
702019 94
70201CG 84
70201HP 84
70202.1 94
702020 98
702021 102
702022 102
702023 103
702024 103
702025 78
702026 81
702027 81
702028 81
702029 81
70203 89
702030 81
702031 81
702032
81, 92
702033
81, 92
702034
81, 92
702035 81
702036 81
702037 81
702038 81
702039 81
70204.36 98
702040 81
702041 82
702042 82
702043 82
702044 83
702045 83
702046 83
702047 83
702048 83
702049 83
70205.36 98
702050 90
702051
90, 93
702052
90, 93
702053 90
702054 94
702055 95
702056 100
702057 101
702058 103
702059 103
70206.36 99
702060 103
702061 103
702062 103
702063
87
702064 87
702065 85
702066
79, 92
702067 79
702068
79, 92
702069 79
702070 80
702071 100
702072 95
702073 103
702074 103
702075 84
702076 84
702077
80, 83, 84, 87
702078
80, 92
702079
80, 92
70208.36 99
702080 81
702081 81
702082 81
702083 81
702084 81
702085 81
702086 99
702088 80
702089
90, 93
70209.1
99
702093 100
702094 100
702096 93
702097 93
702098 93
702099 93
70210.36 99
702100 99
702101 101
702101HP 86
702102 103
702103
90, 93
702104 93
702105 93
702106 93
702107 81
702109 81
702110 95
702112 100
70212 78
70212.1 78
702128 102
702129 102
70213
79, 92
70213.2
79, 92
702130 103
702131 103
702132 102
702133 102
702134
85, 87
702135 80
702141 85
70214CG 84
70215.36 98
70216.36 98
70217.36 99
702201 82
702202 82
702203 82
702204 82
702205 82
702206 82
702207 82
702208 82
702209 82
702210 91
702211 82
702212 82
702213 82
702214 82
702215 86
702216 86
702217 86
702218 86
702219 86
702220 91
702221 86
702222 86
702223 86
702224 86
702225 88
702226 82
702227 88
702228 88
702229 88
702230 91
702231 88
702232 88
702233 88
702234 88
702235 88
702236 88
702237 88
702240 91
306
info@mn-net.com
REF Page
REF Page
702261 99
702263 99
702280 91
702282
80, 92
702283
80, 92
702284
80, 92
702286 81
702287 81
702287.1 81
702288 81
702288.1 81
702292
90, 91
702293
80, 92
702300 91
70231 85
70231.1 85
70231.2 85
70231.3 85
70231.4 85
702311 93
70232 89
70233 100
70234 100
70234.8 100
70234.9 100
70235 101
70236 100
70236.1 100
70237 100
70238 101
702401
85, 87
702437 79
70245 79
70248 80
702481 80
70249 79
70250 79
70251 78
702514 88
702515 89
702516 100
70252.1 78
70254 99
702540 99
702541 99
70255 89
70256 85
70257 89
70258 89
70260 90
702602 297
702604 297
702605 297
702606 297
702609 297
70261 80
702610 297
702611 297
702612 297
702613 297
702619 297
70262 85
702620 297
702621 297
702622 297
702623 297
702625 297
702626 297
702627 297
702628 297
70263 85
702632 297
70264 85
70266 102
70267 102
70269 102
702709 87
70271 94
702710 87
702710.1 87
702712 87
702713 87
702714 87
702716
80, 83, 84, 87
702717.2
87
702718 87
702718.1 87
702719 88
70272 94
70273 94
702730 85
702731 87
702732 81
70274 94
70275 94
70277 101
702773 100
702774 101
702775 100
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80, 83, 84, 87
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80, 83, 84, 87
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79, 84
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80, 83, 84, 87
702826 95
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79, 84
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90, 93
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702D20TB 101
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723706.10 281
723706.25 281
723707.10 281
723707.25 281
723708.10 281
723708.25 281
723709.10 281
723709.25 281
723710.10 281
723710.25 281
723711.10 281
723711.25 281
723712.10 281
723712.25 281
723713.10 281
723713.25 281
723714.10 281
723714.25 281
723790.1 280
723790.2 280
723791.1 280
723791.2 280
723792.1 280
723792.2 280
723793.1 280
723793.2 280
723827.10 265
723827.25 265
723827.50 265
723830.10 265
723830.25 265
723830.50 265
723890.100 277
723936.25 265
723936.50 265
723945.25 278
723945.50 278
724243 295
724244 295
724333 295
724334 295
724335 295
724336 295
724337 295
724405 295
724406 295
724407 295
724408 295
724409 295
724410 295
724411 295
724412 295
724413 295
724414 295
724415 295
724416 295
724417 295
724418 295
724421 295
724422 295
724423 295
724424 295
725801.25 244
725801.50 244
725802.30 244
725802.60 244
725805.15 244
725805.30 244
725805.60 244
725806.30 244
725806.60 244
725807.30 244
725807.60 244
725810.25 247
725810.50 247
725811.30 247
725811.60 247
725812.25 247
725812.60 247
725813.30 247
725815.12 247
725815.50 247
725820.15 247
725820.30 247
725820.60 247
725825.30 247
725825.60 247
725826.30 247
725826.60 247
725850.30 248
725850.60 248
726022.15 255
726022.25 255
726022.30 255
726022.50 255
726022.60 255
726024.10
242, 267
726024.20
242, 267
726025.20
242, 267
726038.10 242
726038.15 242
726038.25 242
726038.30 242
726038.60 242
726050.10 242
726050.15 242
726050.25 242
726050.30 242
726050.50 242
726050.60 242
726056.10 245
726056.15 245
726056.25 245
726056.30 245
726056.50 245
726056.60 245
726058.10 253
726058.15 253
726058.25 253
726058.30 253
726058.50 253
726058.60 253
726064.30 253
726064.60 253
726065.12 255
726065.25 255
726065.50 255
726066.25 255
726066.50 255
726067.25 255
726067.30 255
726067.50 255
726067.60 255
726080.10
258, 267
726081.10 242
726081.25 242
726081.30 242
726081.50 242
726081.60 242
726089.30 259
726089.50 259
726089.60 259
726090.30 259
726090.60 259
726091.25 259
726091.30 259
726091.50 259
726091.60 259
726095.30 259
726095.60 259
726096.30 259
726096.60 259
726099.25 245
726099.30 245
726099.50 245
726099.60 245
726102.15 275
726102.30 275
726104.15 275
726104.30 275
726106.15 275
726106.30 275
726108.15 275
726108.30 275
726116.25 262
726116.30 262
726116.50 262
726116.60 262
726118.15 258
726118.25 258
726118.30 258
726118.50 258
726118.60 258
726131.10 274
726131.25 274
726132.10 274
726132.25 274
726133.10 274
726133.25 274
726154.30 254
726154.60 254
726157.30 254
726157.60 254
726162.30 256
726162.60 256
726165.30 256
726165.60 256
726180.10
262, 267
726201.25 243
726201.50 243
726202.30 243
726202.60 243
726203.12 243
726205.15 243
726205.30 243
726205.60 243
312
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726210.12 246
726210.25 246
726210.50 246
726211.30 246
726212.25 246
726212.50 246
726212.60 246
726213.30 246
726215.12 246
726215.25 246
726215.50 246
726220.15 246
726220.30 246
726220.60 246
726225.30 246
726225.60 246
726226.30 246
7262267.60 246
726241.30 263
726241.60 263
726242.30 263
726242.60 263
726243.30 263
726243.60 263
726246.30 263
726246.60 263
726296.25 260
726296.30 260
726296.50 260
726296.60 260
726301.10 242
726301.25 242
726301.30 242
726301.50 242
726301.60 242
726302.10 242
726302.15 242
726302.25 242
726302.30 242
726302.50 242
726302.60 242
726304.10 242
726304.25 242
726304.30 242
726304.50 242
726304.60 242
726313.10 245
726313.15 245
726313.25 245
726313.30 245
726313.50 245
726313.60 245
726314.15 245
726314.25 245
726314.30 245
726314.50 245
726314.60 245
726316.25 245
726316.30 245
726316.50 245
726316.60 245
726318.10 253
726318.15 253
726318.25 253
726318.30 253
726318.50 253
726318.60 253
726320.25 253
726320.30 253
726320.50 253
726320.60 253
726321.25 260
726321.30 260
726321.50 260
726321.60 260
726323.10 242
726323.15 242
726323.25 242
726323.30 242
726323.50 242
726323.60 242
726325.15 245
726325.25 245
726325.30 245
726325.50 245
726325.60 245
726341.25 262
726341.30 262
726341.50 262
726341.60 262
726344.25 262
726344.30 262
726344.50 262
726345.30 262
726346.25 262
726351.25 255
726351.30 255
726351.50 255
726351.60 255
726352.25 258
726352.30 258
726352.50 258
726352.60 258
726353.30 276
726354.30 276
726355.25 276
726356.30 276
726357.30 276
726358.30 276
726359.30 276
726360.30 276
726361.10 267
726361.10 276
726380.30 261
726380.60 261
726381.30 261
726381.60 261
726382.30 261
726382.60 261
726383.30 261
726383.60 261
726400.25 249
726400.50 249
726410.10
249, 267
726410.20
249, 267
726420.30 249
726420.60 249
726421.30 249
726440.30 249
726440.60 249
726441.30 249
726441.60 249
726442.30 249
726442.60 249
726443.30 249
726465.25 250
726465.50 250
726470.30 250
726470.60 250
726480.30 250
726480.60 250
726481.30 250
726481.60 250
726482.30 250
726482.60 250
726483.30 250
726490.10
250, 267
726519.15 242
726519.25 242
726519.30 242
726521.10 242
726521.25 242
726521.30 242
726521.50 242
726523.10 245
726523.25 245
726523.30 245
726525.10 245
726525.25 245
726525.30 245
726525.50 245
726525.60 245
726529.10 242
726529.15 242
726529.25 242
726529.30 242
726541.10 245
726541.15 245
726541.25 245
726541.30 245
726545.10 253
726545.15 253
726545.25 253
726545.30 253
726548.30 260
726549.25 260
726549.30 260
726600.25 260
726600.30 260
726600.50 260
726600.60 260
726623.25 245
726623.30 245
726623.50 245
726623.60 245
726628.25 245
726628.30 245
726628.50 245
726628.60 245
726735.15 253
726735.25 253
726735.30 253
726735.50 253
726742.25 255
726742.30 255
726742.50 255
726742.60 255
726744.25 255
726744.30 255
726744.50 255
726744.60 255
726747.10 255
726747.15 255
726747.25 255
726747.30 255
726755.30 255
726757.25 255
726757.30 255
726757.50 255
726757.60 255
726771.25 251
726771.30 251
726771.50 251
726771.60 251
726777.25 251
726777.30 251
726777.60 251
726780.30 251
726780.50 251
726780.60 251
726783.25 251
726784.25 252
726785.25 252
726785.30 252
726785.50 252
726785.60 252
726786.30 252
726786.50 252
726787.25 252
726787.30 252
726787.50 252
726787.60 252
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Appendices
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726789.25 252
726789.30 252
726802.25 242
726802.30 242
726802.50 242
726802.60 242
726805.25 242
726805.30 242
726805.50 242
726805.60 242
726807.25 245
726807.30 245
726807.50 245
726807.60 245
726809.25 245
726809.30 245
726809.50 245
726809.60 245
726821.25 242
726821.30 242
726821.50 242
726821.60 242
726824.25 253
726824.30 253
726824.50 253
726824.60 253
726832.25 242
726834.12 242
726834.25 242
726834.50 242
726837.12 242
726837.25 242
726837.50 242
726839.50 242
726841.25 253
726841.50 253
726846.10
245, 267
726848.10
255, 267
726854.25 245
726857.25 245
726857.50 245
726860.25 245
726860.50 245
726863.25 245
726863.50 245
726871.15 257
726871.25 257
726871.30 257
726871.50 257
726871.60 257
726874.30 257
726874.50 257
726874.60 257
726877.15 257
726877.30 257
726877.50 257
726877.60 257
726880.25 257
726880.30 257
726880.50 257
726880.60 257
726900.30 273
726903.10 273
726905.30 273
726911.25 245
726911.30 245
726911.50 245
726911.60 245
726916.10 245
726916.25 245
726916.30 245
726916.50 245
726926.10 242
726926.25 242
726926.30 242
726926.50 242
726929.25 253
726929.30 253
726929.50 253
726929.60 253
726931.10 242
726931.25 242
726931.30 242
726931.50 242
726934.15 245
726934.25 245
726934.30 245
726934.50 245
726965.30 253
727400 59
727401 59
727402 59
727403 59
727404 59
727405 59
727406 59
727407 59
727420 59
727421 59
727422 59
727423 59
727424 59
727450 59
727451
59
728025.20 166
728025.30 166
728025.40 166
728025.46 166
728026.20 166
728026.30 166
728026.40 166
728026.46 166
728027.46 166
728028.20 166
728028.30 166
728028.40 166
728028.46 166
728029.20 166
728029.30 166
728029.40 166
728029.46 166
728030.46 166
728031.20 166
728031.30 166
728031.40 166
728031.46 166
728032.20 166
728032.30 166
728032.40 166
728032.46 166
728033.46 166
728034.20 166
728034.30 166
728034.40 166
728034.46 166
728035.20 166
728035.30 166
728035.40 166
728035.46 166
728036.46 166
728037.20 166
728037.30 166
728037.40 166
728037.46 166
728038.20 166
728038.30 166
728038.40 166
728038.46 166
728039.46 166
728051.30 166
728051.30 166
728051.40 166
728051.40 166
728052.30 166
728052.30 166
728052.40 166
728052.40 166
728053.30 166
728053.30 166
728053.40 166
728053.40 166
728054.30 166
728054.30 166
728054.40 166
728054.40 166
728055.30 166
728055.30 166
728055.40 166
728055.40 166
728777.20 190
728777.30 190
728778.20 190
728778.30 190
729004 70
729004.400 70
729006 70
729006.400 70
729007 70
729007.400 70
729008 70
729008.800 70
729009 70
729009.800 70
729010 72
729011 72
729012 72
729012.400 72
729013 72
729013.400 72
729014 70
729015 70
729020 69
729020.400 69
729021 69
729021.400 69
729022 69
729022.800 69
729023 69
729023.800 69
729024 71
729025 71
729026 71
729026.400 71
729027 71
729027.400 71
729028 73
729028.400 73
729030 69
729030.400 69
729031 69
729031.400 69
729032 68
729032.400 68
729033 68
729033.400 68
729034 73
729036 69
729036.800 69
729037 69
729037.800 69
729039 68
729039.400 68
729043 72
729043.800 72
729044 72
729044.800 72
729048 72
729048.800 72
729049 72
729049.800 72
729050 68
729050.400 68
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729051 68
729051.400 68
729052 71
729053 71
729054 71
729054.800 71
729055 71
729055.800 71
729100 76
729101 76
729102 76
729204 70
729204.400 70
729205 70
729205.400 70
729206 70
729206.400 70
729207 70
729207.400 70
729212 72
729212.400 72
729213 72
729213.400 72
729218 72
729218.400 72
729219 72
729219.400 72
729220 69
729220.400 69
729221 69
729221.400 69
729226 71
729226.400 71
729227 71
729227.400 71
729228 73
729228.400 73
729229 69
729229.400 69
729230 69
729230.400 69
729231 69
729231.400 69
729240 71
729240.400 71
729241 71
729241.400 71
729242 71
729242.400 71
729247 70
729247.400 70
730001 24
730002 24
730003 24
730003.250 24
730003G 24
730004 24
730004.250 24
730004G 24
730005 24
730005.250 24
730005G 24
730007 34
730008 24
730009 24
730010 23
730011 23
730011MPS 53
730012 23
730012G 23
730012MPS 53
730013 23
730013.250 23
730013G 23
730013MPS 53
730014 23
730014.250 23
730014G 23
730015 23
730015.250 23
730015G 23
730016 23
730017 33
730018 23
730020 33
730020G 33
730021 26
730022 26
730023 26
730024 26
730024G 26
730026 52
730028 24
730031 31
730033 31
730033.250 31
730033G 31
730034 52
730049 16
730049P45 16
730051 30
730053 30
730061 30
730063 30
730068.320 75
730068.345 75
730068.620 75
730068.645 75
730070 32
730070G 32
730071 32
730071ASP 53
730072 32
730073 32
730073.250 32
730073ASP 53
730073G 32
730073MPS 53
730075 32
730075.250 32
730075ASP 53
730075G 32
730075MPS 53
730076 36
730077 36
730077.250 36
730078 37
730079 37
730079.250 37
730081 34
730082 34
730082.250 34
730082G 34
730083 29
730084 29
730085 46
730085.250 46
730085G 46
730100.4 52
730101 52
730102 52
730103 52
730104.4 52
730105 52
730106 51
730107 32
730107.250 32
730107G 32
730108 21
730108.250 21
730108G 21
730109 45
730111 21
730111.AOX 41
730111G 21
730112 44
730117 21
730118 21
730118G 21
730119 21
730119.AOX 41
730125 46
730126 34
730127 34
730130 24
730132 45
730132.250 45
730134 26
730135 44
730135.250 44
730135G 44
730139 33
730139G 33
730141 23
730149 45
730149.250 45
730149G 45
730150 50
730151 50
730152 51
730153 51
730154 51
730157 51
730158 52
730159 52
730160 52
730161 52
730162 52
730163 52
730164 52
730166 42
730166G 42
730168 38
730168.250 38
730169 27
730171 52
730172 52
730173 50
730174 50
730175 50
730176 50
730177 50
730178 50
730179 51
730180 31
730180G 31
730183.12 51
730184.12 51
730185 51
730187 51
730188 51
730189.1 51
730189.12 51
730191 52
730192 52
730194 51
730197 7
730199 7
730205 7
730206 7
730207 7
730208 7
730209 7
730212 36
730214 32
730214G 32
730214MPS 53
730217 32
730221 27
730223 49
730225 27
730227 27
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Appendices
REF Page
315
Appendices
REF Page
REF Page
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730230 52
730233 51
730235 45
730238 34
730238G 34
730239 34
730239G 34
730243 51
730249 43
730249G 43
730249G.250 43
730250 33
730259 23
730261 24
730269 23
730275 36
730280 21
730288 7
730294 25
730295 25
730296 25
730296G 25
730297 25
730297G 25
730298 25
730298G 25
730299 25
730299G 25
730300 25
730300G 25
730301 25
730302 25
730310 22
730315 41
730322 37
730323 37
730344 22
730349 7
730350.4 52
730351 52
730355 52
730356 52
730360 50
730365 50
730366 51
730376 22
730377 22
730378 22
730380 52
730381 52
730382 52
730384 34
730385 52
730386 51
730387 51
730388 52
730389 52
730394 22
730396 22
730400 24
730402 24
730403 24
730404 23
730405 23
730406 32
730409 27
730410 27
730411 29
730413 31
730417 30
730418 30
730420 30
730421 30
730425 36
730426 37
730429 33
730442 28
730443 28
730444 28
730446 33
730447 33
730452 33
730453 33
730455 33
730457 34
730458 40
730460 35
730462 35
730464 35
730466 33
730467 33
730473 32
730474 52
730475 52
730482 35
730483 35
730484 35
730487 57
730487.250 57
730489 57
730490 7
730491 7
730492 7
730493 7
730494 7
730496 7
730501 57
730502 57
730505 57
730506 57
730507 57
730507.100 57
730508 57
730509 57
730517.3100 75
730517.6100 75
730533 47
730561 49
730562 49
730564 51
730566 52
730570.320 75
730570.345 75
730570.620 75
730570.645 75
730578.320 75
730578.345 75
730578.620 75
730578.645 75
730579.320 75
730579.345 75
730579.620 75
730579.645 75
730585 57
730586 57
730595.1000 57
730595.500 57
730595.5000 57
730601 26
730602 24
730603 31
730605 30
730606 29
730607 30
730608 32
730609 36
730610 37
730611 23
730612 24
730613 23
730615 21
730616 42
730618 43
730618G 43
730619 45
730620 43
730620G 43
730622 34
730626 31
730626G 31
730628 25
730629 35
730630 35
730631 28
730640 33
730641 33
730642 33
730651 27
730652 27
730653 49
730653.20 49
730660 34
730661 20
730663 15
730664 16
730666 62
730667 62
730671 17
730673 19
730674 18
730680 39
730681 38
730682 38
730683 39
730684 38
730685 39
730686 39
730690 22
730692 22
730693 22
730710 58
730712 58
730714 58
730716 58
730718 58
730720 58
730722 58
730723 7
730724 58
730726 7
730727 17
730727P45 17
730728 19
730728P45 19
730729 19
730729P45 19
730730 58
730731 18
730731P45 18
730732 58
730733 18
730733P45 18
730734 58
730735 18
730735P45 18
730736 58
730737 18
730738 58
730739 18
730739P45 18
730740 58
730741 18
730742 58
730743 18
730744 19
730745 19
730747 19
730747P45 19
730748 19
730748P45 19
730749 19
730751 20
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730752 20
730753 20
730754 20
730754.250 20
730755 20
730755.250 20
730756 20
730757 20
730758 20
730759 20
730765 22
730767 62
730768 62
730798 60
730880 62
730881 62
730884 62
730885 62
730886 62
730887 62
730888 62
730890 62
730891 62
730892 62
730915 62
730931 15
730931.250 15
730931MPS 53
730931P45 15
730934 15
730934P45 15
730935 15
730935MPS 53
730935P45 15
730936 15
730936P45 15
730937 15
730938 15
730938.250 15
730939 15
730939.250 15
730939MPS 53
730940 15
730941 15
730950 17
730950P45 17
730951 17
730951P45 17
730952 16
730952P45 16
730953 16
730954 17
730955 16
730956 16
730956P45 16
730957 16
730958 17
730966 17
730968 17
730968P45 17
730969 16
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762311.500 135
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762330.320 142
762371.400 129
762372.100 129
762372.210 129
762373.100 129
762373.210 129
762373.320 129
762373.400 129
762375.100 129
762375.210 129
762390.160 129
762390.80 129
762392.320 129
762403.100 121
762403.210 121
762403.320 121
762403.400 121
762404.100 121
762404.210 121
762405.100 121
762405.210 121
762406.400 121
762420.160 121
762420.80 121
762422.320 121
762551.100 132
762551.210 132
762553.210 132
762553.320 132
762553.500 132
762554.100 132
762554.210 132
762555.320 132
762555.400 132
762555.500 132
762556.100 132
762556.210 132
762556.320 132
762556.400 132
762556.500 132
762561.100 132
762561.210 132
762563.210 132
762563.320 132
762563.500 132
762564.100 132
762564.210 132
762565.320 132
762565.400 132
762565.500 132
762566.100 132
762566.210 132
762566.320 132
762566.400 132
762566.500 132
762571.100 132
762571.210 132
762573.210 132
762573.320 132
762573.500 132
762574.100 132
762574.210 132
762575.320 132
762575.400 132
762575.500 132
762576.100 132
762576.210 132
762576.320 132
762576.400 132
762576.500 132
762591.160 132
762591.160 132
762591.160 132
762591.80 132
762591.80 132
762591.80 132
762592.320 132
762592.320 132
762592.320 132
762592.500 132
762592.500 132
762592.500 132
763132.20 149
763132.30 149
763132.40 149
763132.46 149
763134.20 149
763134.30 149
763134.40 149
763134.46 149
763136.20 149
763136.30 149
763136.40 149
763136.46 149
763138.20 149
763138.30 149
763332.20 153
763332.30 153
763332.40 153
763332.46 153
763334.20 153
763334.30 153
763334.40 153
763334.46 153
763336.20 153
763336.30 153
763336.40 153
763336.46 153
763338.20 153
763338.30 153
763532.20 151
763532.30 151
763532.40 151
763532.46 151
763534.20 151
763534.30 151
763534.40 151
763534.46 151
763536.20 151
763536.30 151
763536.40 151
763536.46 151
763538.20 151
763538.30 151
801011 225
801013 225
801022 225
801023 225
801033 226
801053 226
801063 226
801113 225
801123 225
802021 224
802022 224
802023 224
803012 226
803013 226
803022 226
803023 226
804022 227
804023 227
805012 215
805013 215
805014 215
805017 215
805021 215
805022 215
805023 215
805024 215
805032 215
805901 215
805902 215
806013 228
806023 228
807021 224
807023 224
807033 224
808013 225
808023 225
808033 225
808043 225
808053 225
808063 225
808072 225
808073 225
809011 215
809012 215
809013 215
809017 215
809017.200 215
809020 215
809021 215
809022 215
809023 215
809027 215
809027.200 215
809028.100 215
809033 227
809043 227
809051 215
809053 215
809061 215
809063 215
809073 215
809083 215
809121 215
809122 215
809123 215
810012 216
810013 216
810022 216
810023 216
810043 229
810053 229
810063 228
811001 223
811011 218
811012 218
811013 218
811021 218
811022 218
811023 218
811032 216
811042 216
811051 228
811052 219
811054 219
811055 227
811056 227
811057 227
811058 227
811059 227
811062 219
811064 219
811071 220
811072 220
811073 220
811074 220
811075 220
811081 220
811082 220
811111 222
811112 222
811115 221
322
info@mn-net.com
REF Page
REF Page
811116 221
811120 223
812003 215
812004 215
812005 215
812005.200 215
812006 215
812007 215
812008 215
812010 218
812011 218
812013 218
812014 218
814000 230
814001 230
814002 230
814003 230
814011 230
814012 230
814013 230
814018 233
814019 233
814021 233
814022 233
814023 233
814024 233
814025 232
814026 232
814027 232
814028 232
814029 230
814030 233
814100 230
814101 233
814102 233
814103 233
814104 233
814200 232
814201 232
814202 232
814203 233
814204 232
814205 232
814206 233
814300 232
814301 232
814302 233
814400 232
814401 233
814402 233
814403 233
814404 233
814405 232
814406 232
814407 232
814500 232
814919 233
814920 233
814921 233
814922 233
814923 233
815010.1 205
815010.25 205
815010.5 205
815020.1 205
815020.25 205
815020.5 205
815030.1 205
815030.25 205
815030.5 205
815050.1 206
815050.25 206
815050.5 206
815060.1 206
815060.25 206
815060.5 206
815070.1 206
815300.1 205
815300.25 205
815300.5 205
815310.1 205
815310.25 205
815310.5 205
815320.1 205
815320.25 205
815320.5 205
815330.1 205
815330.25 205
815330.5 205
815340.1 205
815340.5 205
815350.1 205
815350.25 205
815350.5 205
815360.1 205
815360.25 205
815360.5 205
815380.1 205
815380.25 205
815380.5 205
815381.1 205
815381.25 205
815381.5 205
815390.1 205
815390.25 205
815390.5 205
815400.1 205
815400.25 205
815400.5 205
815410.1 205
815430.1 205
815510.1 205
815510.5 205
815530.1 205
815530.5 205
815540.1 205
815540.5 205
815550.1 205
815550.5 205
815560.1 205
815560.5 205
815600.1 206
815610.1 206
815620.1 206
815650.1 205
815650.25 205
815650.5 205
815710.1 206
815710.5 206
816250.1 234
816250.5 234
816310.1 234
816310.5 234
816320.1 234
816320.5 234
816330.1 234
816330.5 234
816340.1 234
816340.5 234
816380.1 234
816380.5 234
816400.1 234
816400.5 234
816410.1 234
816410.5 234
816610.1 234
816620.1 234
816710.01 234
816720.01 234
817001 229
817002 229
817003 229
817004 229
817005 229
817006 229
817007 229
817008 229
818001 223
818023 224
818024 224
818030.20 215
818032 215
818033 215
818129 215
818130.20 215
818131 215
818132 215
818133 215
818141 218
818143 218
818144 220
818145 220
818146 220
818147 220
818152 220
818153 225
818155 225
818156 225
818157 225
818160 215
818161 215
818162 215
818163 215
818171 220
818182 222
818184 221
818230.20 214
818232 214
818233 214
818240 218
818241 218
818261 214
818329 214
818330.20 214
818331 214
818332 214
818333 214
818342 218
818343 218
818360 214
818362 214
818412 216
818413 216
818422 216
818423 216
818432 216
818442 216
818666 233
821005 213
821010 213
821010.200 213
821015 213
821020 213
821025 213
821030 213
821040 213
821040.200 213
821050 213
821060 213
821110 217
821120 217
821140 217
821150 217
www.mn-net.com
Appendices
REF Page
323
Trademarks
MACHEREY-NAGEL trademarks
ALUGRAM
coated aluminium sheets for TLC
CHROMABOND columns for solid phase extraction (SPE)
CHROMAFIL
syringe filters (membrane filters)
CHROMAFIX
cartridges for solid phase extraction (SPE)
ChromCart
cartridge system for HPLC
LIPODEX
fused silica capillary columns with cyclodextrin phases for GC enantiomer separation
NUCLEODUR
spherical high purity silica for HPLC
NUCLEOGEL
polymer-based HPLC columns
NUCLEOGEN
HPLC ion exchange columns for nucleic acid analyses
NUCLEOSHELL core-shell silica phases for HPLC
NUCLEOSIL
spherical standard silica for HPLC
OPTIMA
fused silica high performance capillary columns with immobilized phases
OPTIMAWAXplus fused silica high performance capillary columns with optimized polyethylene glycol phase
PERMABOND
fused silica capillary columns with immobilized phases
POLYGOSIL
irregular silica for HPLC
POLYGRAM
coated polyester sheets for TLC
Appendices
Accubond
Acquity
Allure
Aqua
Ascentis
Atlantis
AutoTrace
AVICEL
Biomek
Biotage
Bond Elut
Celite
Cheminert
ChiralCel
ChiralPak
Clean Screen
CLEAN-UP
CombiFlash
Companion
Discovery
Duran
epMotion
Eurocel
EXtrelut
Fiolax
Florisil
Gemini
Hypersil
HyPurity
Inertsil
Isco
Isolute
324
Kromasil
LiChrolut
LiChrospher
Luna
Microlab
MultiProbe
Oasis
Polaris
ProntoSil
Purospher
Pyrex
Quadra 3
RapidTrace
Rxi
Rtx
Sep-Pak
SOTAX
Spherisorb
Stabilwax
Styre Screen
Superspher
Symmetry
Synergi
Vespel
VICI
Viton
Xterra
YMC
ZIC
Zorbax
Zymark
Zymate
Trademarks
Common law trademarks ()
AmyCoat
ASPEC
AT
Bakerbond
Benchmate
BPX
Carbowax
CelluCoat
Chem Elut
DB
Equity
FlashMaster
Flash 12i
Focus
Genesis
Hydromatrix
HyperSep
Hypersil
HyPURITY
Kinetex
Lux
Obelisc
Nukol
PEEK
Porapak
Poroshell
SPB
Select
Sequant
Strata
SunFire
Supelclean
Supelcosil
Supelcowax
SymmetryShield
Disclaimer
Regarding these products or services we can not grant any guarantees regarding selection, efficiency or operation.
MACHEREY-NAGEL chromatography products are intended, developed, designed and sold FOR RESEARCH
AND DEVELOPMENT PURPOSES AND ANALYTICAL QUALITY CONTROL / ROUTINE MEASUREMENTS ONLY, except, however, any other function of the product being EXPRESSLY set forth in original MACHEREY-NAGEL
product leaflets.
MACHEREY-NAGEL products are intended for GENERAL LABORATORY USE ONLY!
MACHEREY-NAGEL products are suited for QUALIFIED PERSONNEL ONLY !
Appendices
All used names and denotations can be brands, trademarks or registered labels of their respective owner also if
they do not have a special denotation. To mention products and brands is only a kind of information, i.e. it does
not offend against trademarks and brands and can not be seen as a kind of recommendation or assessment.
MACHEREY-NAGEL products shall in any event be used wearing adequate PROTECTIVE CLOTHING.
For detailed information please refer to the respective Material Safety Data Sheet of the product !
MACHEREY-NAGEL products shall exclusively be used in an ADEQUATE TEST ENVIRONMENT.
MACHEREY-NAGEL does not assume any responsibility for damages due to improper application, abuse, misuse, storage or maintance of our products. Prior to application the user has to read carefully and understand
the instruction or product leaflets included in the product package (if applicable or available on the webpage)
- in case of any doubts the customer has to contact MACHEREY-NAGEL.
Application on the human body is STRICTLY FORBIDDEN. The respective user is liable for any and all damages
resulting from such application.
The user has to ensure that the products used are suitable for the intended application.
MACHEREY-NAGEL does not warrant the reproducibility of published applications.
Image rights: Alexander Maier (19), Alexander Raths (13), anderssehen (46), anutka (221), Birgit Reitz-Hofmann (49), christian42 (277), Ewa Walicka (47, 115), eyeami (13),
Falko Matte (178), Filipebvarela (13, 31, 115, 126), Graa Victoria (224), hfng (275), idrutu (114), isma (39, 114), LUNAMARINA (25, 115), MAK (228), Marcel Nijhuis (29, 115),
maxi (57, 115), Michael Kempf (13, 114), Olga Langerova (185), ollirg (169), Otmar Smit (41, 114), Ramn Lpez (13, 115), Rebel (223), Sebastian Duda (13), SibylleMohn (188),
Stefan Redel (187), Stihl024 (49), Sven Hoppe (13, 114, 184), sylada (49), Tomislav (37, 114), Worytko Pawel (49) fotolia.de
www.mn-net.com
325
Appendices
Code
USP L1
Specification
Octadecyl silane chemically bonded to porous silica particles,
1.8 to 10m diameter
USP L3
USP L7
USP L8
USP L9
USP L10
USP L11
USP L14
USP L16
USP L17
USP L19
USP L20
USP L21
USP L22
USP L23
USP L26
USP L32
USP L34
USP L36
USP L40
USP L43
USP L45
326
MN HPLC phases
NUCLEODUR C18 ec
NUCLEODUR C18 Gravity
NUCLEODUR C18 HTec
NUCLEODUR C18 Isis
NUCLEODUR C18 PAH
NUCLEODUR C18 Pyramid
NUCLEODUR PolarTec
NUCLEODUR Sphinx RP
NUCLEOSHELL RP 18
NUCLEOSIL C18
NUCLEOSIL C18 AB
NUCLEOSIL C18 HD
NUCLEOSIL C18 MPN
NUCLEOSIL C18 PAH
NUCLEOSIL C18 PPN
NUCLEODUR SiOH
NUCLEOSIL SiOH
NUCLEODUR C8 ec
NUCLEODUR C8 Gravity
NUCLEOSIL C8
NUCLEOSIL C8 HD
NUCLEODUR NH2/NH2-RP
NUCLEOSIL Carbohydrate
NUCLEOSIL NH2/NH2-RP
NUCLEOSIL SA
Page
133
116
130
120
168
122
124
128
148
157
144
158
182
170
183
142
165
133
116
160
161
161
185
163
164
NUCLEODUR CN/CN-RP
NUCLEOSIL CN/CN-RP
NUCLEODUR Sphinx RP
NUCLEOSIL C6H5
NUCLEOSIL SB
138
164
128
162
165
NUCLEOSIL C2
NUCLEOGEL ION 300 OA
NUCLEOGEL SUGAR 810 H
NUCLEOGEL SUGAR 810 Ca
NUCLEOGEL SUGAR Ca
NUCLEOSIL OH (Diol)
162
187
186
186
187
162
NUCLEOGEL RP
NUCLEOGEL SCX
184
181
NUCLEOGEL SAX
181
NUCLEOSIL C4
NUCLEOSIL C4 MPN
NUCLEOSIL CHIRAL-1
161
182
176
NUCLEOGEL SUGAR Pb
187
NUCLEOSIL CHIRAL-3
177
NUCLEOCEL DELTA
174
NUCLEODUR PFP
126
150
172
NUCLEOSHELL PFP
NUCLEODEX -OH, -PM
info@mn-net.com
Specification
Strong cation exchange resin consisting of sulfonated cross-linked PS/DVB
copolymer in the Na form, ~ 7 to 11m particles
Spherical porous silica gel, covalenty modified with alkyl amide groups and
endcapped, 3 or 5m particles
Specification
Dimethylpolysiloxane oil
USP G3
USP G6
USP G7
USP G16
Trifluoropropylmethylpolysiloxane
50% 3-cyanopropyl 50% phenylmethylpolysiloxane
Polyethylene glycol (av. mol. weight ~ 15000); a high molecular weight
compound of polyethylene glycol and a diepoxide
USP G19
USP G25
USP G27
USP G28
USP G32
USP G35
USP G36
1% vinyl 5% phenylmethylpolysiloxane
USP G38
Dimethylpolysiloxane oil
USP G42
USP G43
USP G46
USP G49
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Page
187
NUCLEODUR PolarTec
NUCLEOSIL C18 Nautilus
124
148
MN GC phases
Page
242
OPTIMA 1
243
OPTIMA 1 MS
244
OPTIMA 1 MS Accent
274
OPTIMA 1-TG
264
PERMABOND SE-30
277
PERMABOND P-100
255
OPTIMA 17
256
OPTIMA 17 MS
274
OPTIMA 17-TG
257
OPTIMA 210
258
OPTIMA 225
260
OPTIMA WAX
261
OPTIMA WAXplus
265
PERMABOND CW 20 M
PERMABOND CW 20 M-DEG 279
FS-CW 20 M-AM
277
258
OPTIMA 225
262
OPTIMA FFAP
263
OPTIMA FFAPplus
265
PERMABOND FFAP
245
OPTIMA 5
276
OPTIMA 5 Amine
275
OPTIMA 5 HT
246
OPTIMA 5 MS
247
OPTIMA 5 MS Accent
264
PERMABOND SE-52
254
OPTIMA 35 MS
254
OPTIMA 35 MS
262
OPTIMA FFAP
263
OPTIMA FFAPplus
265
PERMABOND FFAP
245
OPTIMA 5
276
OPTIMA 5 Amine
275
OPTIMA 5 HT
246
OPTIMA 5 MS
247
OPTIMA 5 MS Accent
278
PERMABOND SE-54 HKW
242
OPTIMA 1
243
OPTIMA 1 MS
244
OPTIMA 1 MS Accent
274
OPTIMA 1-TG
264
PERMABOND SE-30
277
PERMABOND P-100
254
OPTIMA 35 MS
251
OPTIMA 1301
252
OPTIMA 624
252
OPTIMA 624 LB
253
OPTIMA 1701
249
OPTIMA -3
Appendices
Code
USP G1/
G2
MN HPLC phases
NUCLEOGEL SUGAR Na
327
Notes
Appendices
328
info@mn-net.com
Filtration
Filter papers
Membrane filters
Extraction thimbles
Rapid Tests
Test papers
Test strips
Urine test strips
Chromatography
Appendices
Water Analysis
HPLC GC DC SPE
Vials and caps
Syringe filters
Bioanalysis
Kits for purification of nucleic acids
Kits for purification of proteins
Transfer membranes
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329
An optimal
chromatography product
from MACHEREY-NAGEL
for every application
local distributor
ChromCat e8/15/0/6.12 PD
Printed in Germany
KATEN200001
www.mn-net.com
MACHEREY-NAGEL
USA:
MACHEREY-NAGEL Inc.
Tel.: +1 484 821 0984
Fax: +1 484 821 1272
E-mail: sales-us@mn-net.com