The epidermis has a high level of metabolic activity due to which the

secretion of different kinds of biomarkers can be used to develop produces

that are non-invasive to measure the skin in order to monitor and measure
disorders and then identify a strategy for their treatment or therapy.

The focus of this report is on the measurement of cytokine. It can be

measured by adopting ELISA assay after the collection of samples of skin.
The level of cytokine in the skin has been measured so far by using lengthy
invasive procedures such as punch biopsy, scrapping and using blister fluid.
Therefore, using ELISA assay has many significant effects because it provides
access to cytokine with the least assault and high accuracy. Therefore the
monitoring of cytokine in disorders related to skin i.e. inflammatory and antiinflammatory or Th1 and Th2 types will facilitate in evaluating the sheerness
of the injury, what kind of harm and the role of the therapy. Till now the
evaluation of the markets is mostly done by using invasive and lengthy
procedures like biopsies of skin and separation of tissues. In recent times,
there has been an attempt to use techniques that can assess these markers
with the least attack, high accuracy and fast measurement. As mentioned
earlier that epidermis contains a high level of metabolic activity therefore the
secretion of different types of biomarkers take place throughout the SC for
example the antioxidants, cytokines, DNA, RNA etc. This secretion however
can be used to make the procedure for measurement of skin that are noninvasive and monitor the disorders of the skin. When the evaluation of these
biomarkers is conducted, the profile obtained will help to identify particular
clinical condition that includes the endogenous diseases and this pattern can
even be used to monitor the effectiveness and efficiency of the treatments.
In addition to this, a different and unique array of network of cutaneous
biomarkers has the potential to be used as a fingerprint for every individual
and hence it can be used to develop cultured, refined and customized
methods for treatments.

TAPE STRIPPING:
Tape Stripping is a procedure that is semi-invasive, and it can be used
for any skin are and also allows to measure the amount of cytokine in the
different layers of SC. In this procedure, a commercial tape strip is chosen.
This strip is usually used in the skin research, and it has a water soluble
adhesive that is often used for the recovery of cytokine from the tape. After
the collection, the tape strip is removed and then the exposed site is stripped
some times till the time SC is completely removed. The strips of tape are
taken from every site and are then placed in vessels made up of glass that
are immediately put on dry ice and then placed for storage at a temperature
of 70-degree centigrade.
TAPE STRIPPING TECHNIQUE:
One of the simplest ways to reduce the barrier that is forced by SC is to
remove it. In theoretical context, an adhesive tape is used for the removal of
the layer of corneocytes but practically in vivo, it is usually done by TS and is
removed through repeated use of the adhesive tapes on the surface of the
skin. This method of tape stripping that uses adhesive tape a modern and
widely accepted method for the examination of the location and spread of
the substances in the stratum corneum (SC). This method has a very less
invasive and removes SC by repeated use of the proper adhesive tapes.