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Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
General notes
Properties
Form
Liquid
Storage instructions
Shipped at 4C. Store at +4C short term (1-2 weeks). Upon delivery aliquot. Store at -20C long term. Avoid
freeze / thaw cycle.
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity
Clonality
Monoclonal
Clone number
EPR13134
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab179457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
Flow Cyt
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
IP
1/10 - 1/100.
Application notes
Target
Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase
(necessary for superoxide production).
Involvement in disease
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive
type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder
characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients
suffer from life-threatening bacterial/fungal infections.
http://www.abcam.com/NCF1-antibody-EPR13134-ab179457.html
Product Datasheet
Sequence similarities
Cellular localization
Cytoplasm.
Secondary
Standard HRP labeled goat anti-rabbit at 1/2000 dilution
developed using the ECL technique
Western blot - Anti-NCF1 [EPR13134] antibody
(ab179457)
http://www.abcam.com/NCF1-antibody-EPR13134-ab179457.html
Product Datasheet
Western blot analysis on Immunoprecipitation pellet from
either 1) Raji cell lysate, or 2) 1xPBS (negative control);
showing NCF1, using ab179457 at 1/10 dilution and
HRP-conjugated anti-rabbit IgG preferentially detecting
the non-reduced form of rabbit IgG.
Please note: All products are "FOR RESEARCH USE ONLYAND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
http://www.abcam.com/NCF1-antibody-EPR13134-ab179457.html