Professional Documents
Culture Documents
BIOCHEMISTRY I
Lecture 1
42
Exam Dates: Friday 9/18/15, Friday 10/16/15, Friday 11/6/15, and Friday 12/4/15.
Final Exam: Wed 12/16/15.
Lecture 1 8/24/15
Lecture 1 8/24/15
Biochemistry:
The study of the chemistry of living things
Chemistry:
Life:
Lecture 1 8/24/15
Lecture 1 8/24/15
Lecture 1 8/24/15
Lecture 1 8/24/15
Lecture 1 8/24/15
10
Lecture 1 8/24/15
11
Cells
12
Prokaryotic Cells
Lecture 1 8/24/15
13
Eukaryotic Cells
5-100 m diameter
More complicated internal structures.
Organelles
Membrane-enclosed
compartments within cytoplasm
Specialized functions
Cytoskeleton
Networks of protein laments
Provide structure, organization to
cytoplasm
Cell shape, motility
Smaller protein subunits which
assemble/disassemble to make
dynamic laments
Multicellular eukaryotes have
dierent cell types with dierent
properties and functions.
Lecture 1 8/24/15
14
Lecture 1 8/24/15
15
Subcellular Fractionation
Dierent compartments in the cell
(cytosol, organelles) contain dierent
molecules.
Organelles (and the proteins and other
molecules they contain) can be separated
from one another by dierential
centrifugation.
Cells are resuspended in an aqueous
solution and homogenized to rupture the
plasma membrane.
The crude homogenate is centrifuged.
Dierent organelles have dierent sizes
and will sediment at dierent rates.
102 Chapter 4 Centrifugation Techniques in Biochemistry
Sucrose-density centrifugation ner
separation of particles based on dierent
ratios of lipid to protein.
FIGURE 4.5
Each fraction can then be studied to
Rotors for a highspeed centrifuge.
characterize its biochemical contents.
A Fixed-angle;
B Swinging-bucket;
C Vertical.
Courtesy of
Beckman Coulter.
Lecture 1 8/24/15