Product Datasheet

Anti-Chloramphenicol antibody ab35658

Overview
Product name

Anti-Chloramphenicol antibody

Description

Sheep polyclonal to Chloramphenicol

Specificity

ab35658 is specific to Chloramphenicol (CAP, Cm).

Tested applications

ELISA, FPIA

Immunogen

Aromatic amino derivative of chloramphenicol (prepared by reduction of the nitro group by high-pressure
hydrogenation) conjugated to KLH using the diazotisation procedure.

Properties
Form

Liquid

Storage instructions

Shipped at 4C. Upon delivery aliquot and store at -20C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide

Constituents: PBS, pH 7.2

Purity

IgG fraction

Purification notes

Prepared by Ammonium Sulphate fractionation.

Clonality

Polyclonal

Isotype

IgG

Applications
Our Abpromise guarantee covers the use of ab35658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application

Abreviews

Notes

ELISA
FPIA

Application notes

ELISA: 1/4000 - 1/500000.

ab35658 was used in a simple indirect ELISA with 400 ng of Chloramphenicol-BSA per well. The titres detailed
above were elucidated using an HRP labelled secondary antibody/TMB detection system.
FPIA: 1/1500.
When tested by polarisation fluoroimmunoassay, the final titre dilution was found to be 0.5 ml tracer + 0.5 ml diluted
antibody. Tracer is aromatic amino derivative of chloramphenicol labelled by reaction with fluorescein isothiocyanate
(10 nmol/L).

Not yet tested in other applications.

Optimal dilutions/concentrations should be determined by the end user.

Target
Relevance

Chloramphenicol is an antibiotic that was derived from the bacterium Streptomyces venezuelae. It was the first
antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of
microorganisms, but due to serious side effects (eg damage to the bone marrow) in humans, it is usually reserved
for the treatment of serious and life threatening infections (eg typhoid fever). It is also used in eye drops or ointment
to treat bacterial conjunctivitis.

Please note: All products are "FOR RESEARCH USE ONLYAND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Product Datasheet
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Terms and conditions

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