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IN R E S E A R C H L A B O R A T O R I E S T H A T U S E H U M A N T I S S U E S
AGENTS
SUMMARY: The personnel in laboratories that utilize tissue and fluids from humans and other primates are at
risk for infection with agents, including the viruses causing hepatitis, AIDS, and other infective agents such as
mycobacteria tuberculosis. To minimize the chance of infection of laboratory personnel, carefully organized
policies and procedures to minimize exposure to infective agents must be established in research laboratories. We
outline some of the approaches of hospital clinical laboratories which have proved most effective in minimizing
transmission of infections from samples to laboratory personnel. Also, we discuss simple considerations important in the use and in the selection of safety equipment. These guidelines and references to other safety information are provided to aid research laboratories in establishing safety procedures that will minimize chances
of personnel contamination with infective agents from research samples.
Key words: laboratory safety; infected human samples; hepatitis; AIDS; HIV; laboratory personnel.
I.
INTRODUCTION
With the accelerated utilization of human tissue in
biological research and with the spread of viral infections within our population, there is an increased risk
of transmission of infection to workers who come in
contact with human tissues and fluids. Personnel
working with human tissues and fluids in clinical settings have been informed of these dangers, and clinical
laboratory workers in hospitals usually have access to
extensive guidelines concerning protective measures
necessary to avoid the transmission of viral and other
infections 11-231.
In contrast, research workers using human tissues are
frequently uninformed as to precautions for avoiding
contamination with infective agents; yet many human
tissues may be obtained from patients with asymptomatic viral or other infections. The literature contains
some general discussions of laboratory safety to prevent
infection with transmissible agents from human tissues.
However, specifics of protective measures are not
described in detail, and most guidelines are published in
clinically related journals.
When the National Cancer Institute funded the
Cooperative Human Tissue Network to increase the
supply of human tissues available for cancer research, it
was mandated that safety guidelines be established for
investigators who received human tissues. In meeting
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M A T E R I A L S AND METHODS
This section is devoted to a general consideration of
safety equipment that is used to reduce the possibility of
transmission of infections to laboratory personnel
working with human and other tissues.
For any piece of safety equipment to be protective, it
must be used. If safety equipment is too complicated or
too uncomfortable, then the utilization of this equipment by laboratory personnel will be reduced. Thus,
with any safety equipment, one must compromise
between ease and comfort of use and its protective
characteristics. Another consideration may be cost.
A. Gloves.
Protective gloves vary considerably in their
strength, comfort, and in their interference with
dexterity of the hands. There is also a large variation
in the cost of protective gloves. The protective glove
should be matched to its use. Thus, for work which
requires a high degree of protection but less dexterity,
a very strong protective glove may be used such as
heavy duty (guage) latex or neoprene latex gloves.
Examples of such jobs are washing contaminated
dishes and instruments, performing autopsies, or
handling tissues when there is a reduced requirement
for manual dexterity. In other uses, such as in
dissection of organs or in tissue preparation, a high
degree of manual dexterity as well as a high degree of
protection may be required. In such cases more expensive, high quality, surgical gloves may be
necessary. Finally, in other uses that require a high
degree of manual dexterity but less risk of puncturing
or tearing the gloves, a glove that permits maximum
dexterity but does not require the great strength or
expense of surgical gloves might be used, such as
disposable latex gloves. Their advantage over surgical
gloves would be one of cost in that surgical gloves may
cost $1-$2 per pair, whereas 100 disposable latex
gloves can be purchased for approximately $15-$20.
An even less expensive glove, but one that offers less
dexterity, is polyethylene or polyvinyl chloride gloves.
All these gloves can be obtained from standard
scientific supply houses (e.g., American Scientific
Preducts) and the final selection of gloves depends on
the balance of ease of use, protection, strength, and
cost.
B. Face protection.
Protection of the face can range from safety glasses
to complete face masks. Contact lenses provide no
protection from splashes and may prevent adequate
cleansing following a splash to the eyes. Thus contact
lenses should never be worn in the laboratory unless
covered by goggles. As a minimum, safety glasses
should be used by all personnel requiring correction of
vision and goggles may be used by other personnel.
Safety glasses and goggles provide no protection for
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III.
PROCEDURE
2. Gloves
a. Wear gloves whenever there is any possibility of
contamination {e.g., handling specimens, test
vessels, or containers), particularly if hands are
cut or abraded. Gloves may become contaminated while handling specimens and
reagents and must not then be used to handle
clean objects te.g., laboratory equipment,
doors, records, etc.}. Discard gloves (into
contaminated waste) immediately after use to
minimize the possibility of contamination.
i. Wear disposable gloves when handling tissue
specimens, when removing plasma or serum
from blood, and when handling any human
fluids or performing any tests that use human
fluids.
ii. Wear gloves while changing dialyzer membranes on automated chemistry equipment or
any other cleaning operation on equipment
when hands are likely to contact residual
human biological specimens or contamination directly.
iii. Do not touch unprotected areas of the body
{e.g., eyes, face, mouth, etc.) while wearing
gloves.
iv. Remove and properly dispose of gloves before
leaving the work area and before touching
telephones, procedures, reports, logs,
doorknobs, etc.
v. If in ususual circumstances it becomes
necessary to touch the telephone, doorknobs,
or other clean objects while wearing gloves,
use a paper towel (discard appropriately) to
handle the equipment and disinfect equipment immediately after use.
vi. Any puncture or tear of the glove renders the
glove useless. Discard the glove and replace
it immediately.
vii. Use disposable gloves only once and
decontaminate reusable gloves after use.
V.
REFERENCES
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