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March 2014
www.ijsret.org
ABSTRACT:
Digital Holographic Microscopy (DHM) provides
the quantitative distribution of the optical path
created by transparent. We used the reference
beam in DHM at an offset angle. Images of object
are generally obtained using photographic
method. The use of angular spectrum method is
also helpful to solve number of problems related
to microscopic.
KEY WORDS:
Two- step process, interference, reconstruction of
the image, digital holography, quantitative phase
imaging, biological specimens, Zero background,
and living cells.
Figure 1
I.
INTRODUCTION:
Holography is a two- step process. First step is
the recording of hologram where the object is
transformed into a photographic record and the
second step is the reconstruction in which the
hologram is transferred into image. A hologram is
the result of interference occurring between two
waves, an object beam which is the light scattered
off the object and a coherent back ground, the
reference beam, where the light is reaching the
photographic plate directly.
Recording of the Hologram:
In fig (1)There are two parts of the laser beam
<1> reference beam goes directly to the
photographic plate <2> The second beam of light
is directly on to The object to be photographed.
These two beams interfere and form interference
fringes. This photograph is known as holograph as
shown in fig (1) but this is negative plate.
In fig (2) reconstruction of the image must be
necessary in order to get real image. This type of
hologram is known as a transmission hologram
since The image is seen by looking Through it.
Figure 2
II.
IMPORTANCE:
In this letter, digital holographic microscopy
(DHM) is very important to get high resolution,
high- fidelity image of quantitative phase contrast
microscopy. In DHM, interferometric technique is
used. DHM is very simple and easy to operate
technique based on fig. (1) and fig. (2) Digital
IJSRET @ 2014
March 2014
www.ijsret.org
Figure 3
IV.
CONCLUSION:
We have demonstrated that a DHM is an efficient
and easy- to operate interferometric technique.
It is used biological environment to visualize
living cells in a culture. Holographic phase
images are obtained with 0.9m diffraction
limited lateral resolution. In DHM photographic
plate, each contains information about the entire
object form even a small part of the hologram the
entire image can be reconstructed if only with a
reduced clarity and definition of the image.
REFERENCES:
(1) M.K.
Kim,
Tomographic
Threedimensional imaging of a biological
specimen using Wavelength- scanning
digital interference holography.
(2) J.W. Goodman, introduction to Fourier
optics.
(3) T.M. Kreis, M. Adams and W.P.O.
Jueptner, Methods of digital holography.
(4) A
text
book
of
optics,
N.
subrahmanyamBrijlal, M.N. Avadhanulu.
(5) U. Schnar and W. P. Jueptner, Digital
Holography.
IJSRET @ 2014