Exercise 2: Skeletal Muscle Physiology: Activity 4: Tetanus in Isolated Skeletal Muscle Lab Report

Pre-lab Quiz Results

You scored 100% by answering 3 out of 3 questions correctly.
1. Stimulus frequency refers to
You correctly answered: b. the rate that stimulating voltage pulses are applied to an isolated whole skeletal muscle.
2. Which of the following distinguishes a state of unfused tetanus from a state of complete (fused) tetanus?
You correctly answered: d. Muscle tension increases and decreases during a state of unfused tetanus.
3. When the stimulus frequency reaches a value beyond which no further increases in force are generated by the muscle,
the muscle has reached its
You correctly answered: a. maximal tetanic tension.

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Experiment Results
Predict Question:
Predict Question: As the stimulus frequency increases further, what will happen to the muscle tension and twitch
appearance with each successive stimulus? Will there be a limit to this response?
Your answer : b. As the stimulus frequency increases, the muscle tension generated by each successive stimulus will
increase. There will be a limit to this increase.
Stop & Think Questions:
What begins to happen at around 80 msec?
You correctly answered: c. unfused tetanus develops
How does the trace at 130 stimuli/sec compare with the trace at 50 stimuli/sec?
You correctly answered: b. Fused tetanus develops at this greater stimulus frequency.
How do the traces with 146150 stimuli per second compare with the trace at 130 stimuli per second?
You correctly answered: d. Maximal tetanic tension develops with these very high stimulation frequencies.
Experiment Data:
Voltage
8.5
8.5
8.5
8.5
8.5
8.5
8.5
8.5

Length
75
75
75
75
75
75
75
75

Stimuli/sec
50
130
140
142
144
146
148
150

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Active Force
5.12
5.88
5.91
5.94
5.94
5.95
5.95
5.95

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Passive Force
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00

Total Force
5.12
5.88
5.91
5.94
5.94
5.95
5.95
5.95

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Post-lab Quiz Results

You scored 100% by answering 3 out of 3 questions correctly.
1. The term tetanus refers to
You correctly answered: b. sustained muscle tension due to very frequent stimuli.
2. Which of the following distinguishes a state of unfused tetanus from a state of complete (fused) tetanus?
You correctly answered: d. Muscle tension increases and decreases between different values for an unfused tetanus.
3. When the stimulus frequency reaches a value beyond which no further increases in force are generated by the muscle,
the muscle has reached its
You correctly answered: a. maximal tetanic tension.

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Review Sheet Results

1. Describe how increasing the stimulus frequency affected the force developed by the isolated whole skeletal muscle in
this activity. How well did the results compare with your prediction?
Your answer:
Cuando la frecuencia de estmulo fue menor, la fuerza estaba en su nivel ms bajo de todos los experimentos. A medida
que el estmulo
frecuencia se increment a 130 estmulos por segundo la fuerza fue aumentado ligeramente, pero fusionado ttanos
desarrollados en la frecuencia ms alta.
Cuando la frecuencia del estmulo se increment a las cantidades de 146 a 150 estmulos por segundo, la fuerza lleg a
un mximo y se produjo la maxima tensin tetnica, donde no hay nuevos aumentos de la fuerza que se produce a partir
de la frecuencia del estmulo adicional. As que mi prediccin fue
correcta, medida que aumenta la frecuencia del estmulo, la tensin muscular generada por cada estmulo sucesiva
aumentar, y llegar a un limite maximo.
2. Indicate what type of force was developed by the isolated skeletal muscle in this activity at the following stimulus
frequencies: at 50 stimuli/sec, at 140 stimuli/sec, and above 146 stimuli/sec.
Your answer:
50 estmulos / s = no fijado ttanos
140 estmulos / s = ttanos fusionados
146 + estmulos / s = mxima tensin tetnica

3. Beyond what stimulus frequency is there no further increase in the peak force? What is the muscle tension called at this
frequency?
Your answer:
Mxima tensin tetnica

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