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The 6th National Biotechnology Congress of Iran

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The 6th National Biotechnology Congress of Iran

13-15 Aug, 2009, Milad Tower Conference Hall, Tehran-Iran

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The 6th National Biotechnology Congress of Iran

13-15 Aug, 2009, Milad Tower Conference Hall, Tehran-Iran

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The 6th National Biotechnology Congress of Iran

13-15 Aug, 2009, Milad Tower Conference Hall, Tehran-Iran

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References:
1.

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17.
18.

Alizadeh, A., Arlat, M., Sarrafi, A., Boucher, C. A., and Barrault, G. 1997. Restriction fragment
length polymorphism analyses of Iranian strains of Xanthomonas campestris from cereals and
grasses. Plant Dis. 81:31-35.
Alizadeh, A.; Rahimian, H. (1989) Bacterial leaf streak of Gramineae in Iran. Bulletin OEPP/EPPO
Bulletin 19, 113-117.
Bradbury, J.F. (1986) Guide to plant pathogenic bacteria. CAB International, Wallingford, UK.
Bragard, C., Singer, E., Alizadeh, A., Vauterin, L., Maraite, H., and Swings, J. 1997. Xanthomonas
translucens from small grains: Diversity and phytopathological relevance. Phytopathology
87:1111-1117.
Conf. Plant Pathogenic Bact., 8th. M. Lemattre, S. Freigoun, K. Rudolph, and J. G. Swings, EDS.
INRA Edition, Paris.
Cowan, S. T. 1974. Cowan and Steels manual for identification of medical bacteria 2nd ed.
Cambridge university press, Cambridge. UK.
Cunfer, B. M. , and Scolari, B. L. (1982) Xanthomonas campestris pv. translucens on triticale and
other small grains. Phytopathology 72:683-686.
Duveiller, E. (1989) Research on Xanthomonas translucens at CIMMYT. Bulletin OEPP/EPPO
Bulletin 19, 97-103.
Dye, D. W. 1968. A taxonomic study of the genus Erwinia I. The amylovora group. N. Z. J. Agric.
Sci. 11, 590-607.
Fahy, P. C. and Hayward, A. C. 1983. Media and methods for isolation and diagnostic tests. In: P.
C. Fahy and G. J. Persley (eds). Plant bacterial diseases, A diagnostic guide. Academic Press,
Sydney. pp. 337-378.
Honeycutt, R. J., Sobral, B. W. S. & McClelland, M. (1995). tRNA intergenic spacers reveal
polymorphisms diagnostic for Xanthomonas albilineans. Microbiology 141, 32293239.
Klement. Z., Farkas, G. L. and Lovrekovich, L. 1963. Hypersensitive Reaction induced by
phytopathogenic bacteria in the Tobacco leaf. Phytopathology 54, 474-477.
Kovacs, N. 1956. Identification of pseudomonas pyocyanea by the Oxidase reaction. Nature (lond.
) 178, 703.
Oliver, D. M. and Bean, P. 1999. Principles and applications of methods for DNA- based typing of
microbial organisme. Journal of Clinical Microbiology. 37, 1661-1669.
Reddy, C.S., Godkin, J. and Johnson, A.G. 1924.
J.Agr. Res. 28: 1039-1040.
Schaad, N. W. , Jones. J. B. , and Chun. W. 2001 Laboratory Guide For The Identification of Plant
Pathogenic Bacteria. 3the ed. APS Press, St. Pual, Minnesot 373 pp.
Stackebrandt E and Goebel BM. 1994. A Natural View of Microbial Biodiversity within Hot
Spring Cyan bacterial Mat Communities. Int. J. Syst. Bacteriol. 44:4:846-849.
Suslow, T. V., Schroth, M. N. and lsaka, M. 1982. Application of a rapid method for gramdifferentiation of palnt pathogenic and saprophytic bacteria without staining. Phytopathology 72,
917-918.

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The 6th National Biotechnology Congress of Iran

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Diversity and phenotypic characterizations of Xanthomonas translucens

cause of leaf streak disease on wheat, barley in Iran
H. Abootalebi*1**, H. Rahimian2, A. Ghasemi3, and F. Rakhshandeh roo1
1- Department of Plant Pathology, Science and Research Branch of Islamic Azad University, Tehran
2- College of Agronomic Sciences, Sari University of Agriculture and Natural Resources, Sari
3- Iranian Research Institute of Plant Protection, Department of Plant Pathology

Abstract
Barley, wheat and ryegrass plants with leaf streak symptoms on them were randomly collected from fields
located in Kerman, Khorasan razavi and Tehran provinces of Iran in two consecutive growing seasons of
2007and 2008, from which 63 bacterial strains were isolated. Physiological and biochemical tests revealed
that. Strains were catalase positive, oxidase and arginine dihydrolase negative and produced H2S from
cysteine. Tests for lecithinase, gelatin liquefaction, and hydrolysis of casein and starch were positive
however indole or acetoin were not produced by the strains. They utilized cellobiose, fructose, galactose,
and trehalose but not adonitol, dulcitol, maltose, mannitole, keto-glutarate or D-tartrate as carbon sources
for growth. Thirteen strains were pathogenic only on Barley remaining strains were pathogenic also on
Wheat and grass. According to the phenotypic tests and Host rang 23 strains were identified as
Xanthomonas translucens pv. hordei and 40 strains were Xanthomonas translucens pv. cerealis. When
enzyme digestion patterns (BamHI, EcoRI, Hind6I, HindIII, MspI and TaqI) of ITS amplification product
were compared, there were no dramatic differences among the strains. The results implicated that the
Iranian bacterial leaf streak disease of cereals comprised only two pathovar, Xanthomonas translucens pv.
hordei and Xanthomonas translucens pv. cerealis are the cause of this disease in Iran.