Institute of Clinical Laboratory Sciences

SILLIMAN UNIVERSITY
Rotational Examination
Microbiology
Set A
Name: ________________________

Score: ___________

Institution: _____________________

Date: ____________

INSTRUCTIONS: Choose the letter of the correct answer from the choices provided under each question. Write your answers on the space before
each item. Superimpositions are strictly not allowed.

___1. Bacteria characterized by the presence of Teichoic Acid and a think Peptidoglycan layer in the cell wall.
a)

Gram Negative Bacteria

c)

Gram Positive Bacteria

b)

Fungi

d)

Archeobacteria

e)

___2. An unusual component in the cell wall of Mycobacteria that results in its inability to be gram stained.

a)

Teichoic Acid

c)

Porins

b)

Mycolic Acid

d)

Murein

e)

___3. What reagent in gram staining differentiates gram positive and gram negative bacteria, and is considered to be the most important
reagent in gram staining.

a)

Crystal Violet

c)

Ethanol

b)

Grams Iodine

d)

Safranin

f)
a)
b)
e)
a)
b)
e)
a)
b)
e)
a)
b)
e)
a)
b)
e)

___4. This is the stage in the bacterial life cycle in which metabolic activities occur but the organisms do not divide.
Lag phase
c) Stationary phase
Log phase
d) Death phase
___5. All are common normal flora found in the urinary tract of females except:
S. agalactiae
c) E. coli
G. vaginalis
d) AOTA
___6. Which of the ff. organisms is NOT found in the respiratory tract of humans?
Neisseria spp.
c) Enterobacter spp.
Streptococcus spp.
d) Staphylococcus spp.
___7. Which of the ff agar plates would NOT be used in the culture of a urine specimen?
Blood Agar
c) Salmonella-Shigella Agar
MacConkey Agar
d) Both A and B
___8. What is the total magnification of the microscope when using the HPO?
400
c) 40
100
d) 1000
___9. What is the true bacterial count/ml if a total 100 organisms were counted on an agar plate inoculated with 0.1ml from a 1:10,000
dilution?
100,000/ml
d) No count should be made because the sample was diluted
1,000,000/ml
making it invalid
10,000,000/ml
___10. It is the complete destruction of all microorganisms including spores.
Sterilization
c) AOTA
Disinfection
d) NOTA
___11. At what temperature can an autoclave kill all organisms?
100 degrees Celsius at 15 PSI
c) 135 degrees Celsius at 10 PSI
121 degrees Celsius at 15 PSI
d) 125 degrees Celsius at 15 PSI

a)
b)
c)
f)
a)
b)
e)
a)
b)

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___12. True antibiotics are substances that can kill or inhibit the growth of susceptible organisms and are produced by:
Bacteria
c) Laboratories
Fungi
d) Both A and B
___13. In Ziehl Neilsen acid fast staining, what acts as the mordant?
Heat
c) Iodine
Acid alcohol
d) Tergitol
___14. Ziehl Neilsen acid fast staining, what do acid fast bacilli look like?
Blue against a red background
c) Blue against a blue background
Red against a blue background
d) Many different colors depending on the type of bacteria
___15. This biosafety cabinet is closed and air enters and exits through HEPA filters.
Class I
c) Class II A
Class II B
d) Class III
___16. What is the most specific test for the identification of S. aureus?
Catalase test
c) Bound coagulase
Culture on MSA
d) Free coagulase
___17. Which test differentiates Staphylococcus from Streptococcus?
Catalase test
c) Blood agar growth
Culture on MSA
d) NOTA
___18. What is the characteristic colony color S. aureus on MSA?
Yellow
c) White
Blue
d) Red
___19. This test is used to differentiate group D strep from other groups.
Bile esculin
c) Hemolytic Pattern
CAMP
d) Acetamide test
___20. This test is used to differentiate Enteric group D from Non-enteric group D strep.
6.5% NaCl tolerance
c) Bile Esculin
Catalase test
d) PYR test
___21. Differentiates S. pyogenes from S. agalactiae
Beta hemolysis on BAP
c) Bile hydrolysis
Bacitracin Susceptibility
d) Coagulase +
___22. The only sugar N. gonorrheae is able to ferment.
Maltose
c) Lactose
Glucose
d) Sucrose
___23. Which of the ff. enterobacteriaceae generally does not ferment lactose?
Escherichia spp.
c) Salmonella spp.
Klebsiella spp.
d) Enterobacter spp
___24. Which of the ff. enterobacteriaceae generally does not produce H 2S in TSI?
Salmonella spp.
c) E. coli
Proteus spp.
d) Edwardsiella spp.
___25. What is the positive reaction of the indole test?
Blue color development after addition of Erlichs rgt.
c) Yellow color development after addition of Erlichs rgt.
Pink color development after addition of Erlichs rgt.
d) Green color development after addition of Erlichs rgt.
___26. What is the positive reaction of the citric acid utilization test?
Blue color of the slant
c) AOTA
Growth on slant
d) NOTA
___27. What is the positive reaction of the Urease test?
No color change
Purple color change
Yellow color change
Blue color change

f)
a)
b)
e)
a)
b)
f)
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b)
e)

___28. In the CAMP test, what is the organism that is inoculated to enhance hemolysis?
S. epidermidis
c) S. sanguis
S.aureus
d) S. campinii
___29. What bacteria produces the V factor?
S. epidermidis
c) S. sanguis
S.aureus
d) S. campinii
___30. This bacteria is commonly transmitted sexually in young females.
Trichomonas vaginalis
c) Neisseria meningitides
E. coli
d) S. saprophyticus
___31. What species of Mycobacterium causes Leprosy?

e)
f) 41-48. Enumerate the four IMViC Tests and their positive reactions.
g)
h)
i)
j)
k)
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m)
n)
o) 49-50. Give two tests used in the work-up of beta-hemolytic strep.
p)
q)
r)

s)