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Fundamental Genetics
Lecture 8
Linkage and
Chromosome Mapping
in Eukaryotes
Linked Genes
Genes located in the same
chromosomes
Initiated by Thomas Morgan and
Alfred Sturtevant
Will not segregate independently
Affected by crossing over
Chromosome Mapping
Determining the distances between genes and the order
of sequence in a chromosome
Uses the frequency of crossing
The shorter the distance between linked genes, the lower the
frequency of crossing-over.
The longer the distance, the higher the frequency of crossing over
to occur.
00.5 %
34.0 %
35.4 %
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Single Crossover
Multiple Crossover
Three-Point Mapping
The percentage of crossing over could be used
to map genes in a chromosome
Three criteria needed for successful mapping:
Genotypes of organisms producing the
crossover gametes must be heterozygous
for all gene loci
Cross must be constructed so that the
genotypes of gametes could be determined
based on the phenotypes of the offspring.
Large number of offspring must be
produced
Traits considered:
1. Body color
Gray(y+)
dominant to
yellow (y)
Steps:
Determine 3 possible orders
w-y-ec (y at the middle)
y-ec- w (ec at the middle)
y-w-ec (w at the middle)
2. Eye color
Red eyes (w+)
dominant to
white (w)
3. Eye shape
Normal (ec+)
dominant to
echinus (ec)
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Total=1109
Coefficient of Coincidence
and Interference
Coefficient of Coincidence (C)
The measure of actual DCOs that occurred
C = Observed DCO / Expected DCO
= 0.078/0.097
= 0.804 or 80.4%
Interference (I)
phenomenon when a crossover event in one region of a
chromosome inhibits a second event to occur in a nearby
region)
I = 1-C
= 1-0.804 = 0.196 or 19.6%
Interpretation: 19.6% of expected DCO did not occur or only
80.4% of expected DCO was observed
Problem 1
A stock of corn homozygous for the recessive linked genes colorless (c), shrunken
(sh), and waxy (wx) was crossed to a stock of homozygous for the dominant wild
type alleles of the genes (+ + +). The F1 plants were then backcrossed to the
homozygous recessive stock. The F2 results were as follows:
Phenotype
Number
Phenotype
Number
+++
17,959
+ + wx
4,455
c sh wx
17,699
c sh +
4,654
+ sh wx
509
+ sh +
20
c++
524
c + wx
12
a.
b.
c.
d.
e.
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Problem 1: Solution
c sh wx
c sh wx
+ + +
+ + +
c sh wx
SCOI
SCOII
DCO
+ + +
c sh wx
+ sh wx
c + +
+ + wx
c sh +
+ sh +
c + wx
Total
=
=
=
=
=
=
=
=
=
c sh wx
+ + +
NCO
Problem 1: Solution
17,959
17,699
509
524
4,455
4,654
20
12
45,832
c sh wx
35,658 = 77.80 %
= 0.0232 or 2.32 %
1,033 = 02.25 %
32
= 00.07 %
= 0.1994 or 19.94 %
Distance between c and wx = 2.32 + 19.94
= 22.26
sh
Problem 2
wx
2.32 mu
9,109 = 19.87 %
Problem 1: Solution
c
19.94 mu
22.26 mu
In a variety of tomato plant, the mutant genes o (oblate fruit), h (hairy fruit), and c
(compound inflorescence) are all located in chromosome 2. These genes are recessive
to their wild type alleles round fruit, hairless and single inflorescence, respectively.
A
testcross mating of an F1 heterozygote for all three genes resulted in the following
phenotypes:
Phenotypes
Number
Phenotypes
Number
+++
73
++c
348
C = (0.0007) / (0.0232)(0.1984)
= 0.1521 or 15.21%
+h+
+hc
96
o++
110
o+c
oh+
306
ohc
63
I = 1-C
= 1-0.1521
= 0.8479 or 84.79 %
Problem 2: Solution
+++
ohc
NCO:
ohc
ohc
o h + = 306
+ + c = 348
DCO:
Problem 2: Solution
+h+ = 2
+oh
c++
NCO:
DCO:
o+c = 2
SCO I:
Find a sequence
that will satisfy
both NCO and
DCO
oh+
++c
+ch
o++
+oh
c++
SCOII:
oh+
++c
+h+
o+c
ohc
ohc
(same as + o h)
(same as c + +)
(same as + + h)
(same as c o +)
+++
o h c (same as c o h)
o + + (same as + o +)
+ h c (same as c + h)
= 306
= 348
= 2
= 2
=
=
=
=
73
63
110
96
Total = 1,000
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Problem 2: Solution
Problem 2: Solution
c
14 cM
21.0 cM
35 cM
C = (0.004) / (0.14)(0.21)
= 0.1361 or 13.61%
I = 1-C
= 1-0.1361
= 0.8639 or 86.39 %
= 35 cM