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INTRODUCTION
Lageneria sphaerica is commonly known as wild melon
and are found in low lying areas in the tropical regions of
the world. The bitter taste of the fruits are attributed to
elastase activity (Ojiako and Igwe, 2007). This plant is a
perennial with a woody rootstock found naturally in
tropical and Southern Africa (Ellof et al., 2007).It grows in
full sun and semi-shade in forest margins, river-banks
and in dry river beds(Jeffrey, 1978). There are variable
reports on the medicinal uses of parts of this plant
(Fagbemi and Oshodi, 1991). The leaves of different
species of Lageneria sphaerica have been reportedly
used as food condiments while the Cucurbitacaes
oilseeds have been employed in domestic activities
(Odoemena, 2005).These oilseeds are good sources of
lipids and proteins with defatted cakes capable of being
Serum lipid profile and liver enzymes of Rats fed Lageneria sphaerica (Wild bottle gourd) supplemented diet
Chinyere et al.
028
Serum lipid profile and liver enzymes of Rats fed Lageneria sphaerica (Wild bottle gourd) supplemented diet
029
Table I. shows the mean weights of rats before and after feeding Lagneria sphearica supplemented diets (g)
GROUPS
Initial
average
weight of rats (g)
Mean change
weight(g)
Group I
Group II
group III
114.552.97
114.706.09
114.857.10
136.966.48
100.255.48
110.884.71
22.413.51
-14.450.61
-4.972.39
in
(ANOVA).Means
were separated using One way
analysis of variance.
RESULTS
From table I. there was an observed reduction in weight
of group II and III rats. Weight reduction was much more
pronounced in group II animals compared to group III and
control rats. Table II shows Serum lipid profiles and liver
enzymes activities of male albino rats fed Lageneria
sphaerica supplemented diet after 35 days. Results
indicates that AST, ALT and ALP activities significantly
increased (P<0.05) in group II rats compared to groups III
and control rats. Similarly triacylglycerol and total protein
in the serum were significantly higher (P>0.05) in the
group II rats than in group III and control. Although serum
total cholesterol levels remained unchanged significantly
(P<0.05) in all the test group and control, HDL level
significantly increased (P<0.05) in group II and III
compared to group I (control) animals. However
increased supplementation of the test diet led to
enhanced reduction (P<0.05) in serum LDL of the test
groups (II and III) compared to control group (I).
Interestingly all the parameters tested after 35 days of
feeding showed similar pattern after 42 and 49 days of
feeding the animals with Lageneria sphaerica
supplemented diet (Tables III and IV). Figures I- VII
show the histologies of livers from test and control
animals after 49 days (7 weeks) of feeding Lageneria
sphaerica supplemented diets. There was an enlarged
central vein (Fig II) and increased vascularity (Fig v) of
liver samples from group II animals after 49 days of
feeding test diets. Also there was evidence of fat
infiltration of liver of group II animals at the end of feeding
regimen. Similarly the group III animals liver samples
showed evidences of vascularization (Fig III) and
enlarged central vein with atropy of hepatocytes (Fig VII).
Statistical Analysis
DISCUSSION
The statistical analysis of result was done using students
package for social sciences(SPSS) and data collected
were
analyzed
using
Analysis
of
Variance
Serum lipid profile and liver enzymes of Rats fed Lageneria sphaerica (Wild bottle gourd) supplemented diet
Chinyere et al.
030
Table 2. shows Serum lipid profiles and selected liver enzymes activities of male albino rats fed Lageneria
sphaerica supplemented diets after 35 days
AST (I/)
ALT (I/)
ALP (I/)
TG(mg/dl)
TP(g/dl)
TC(mg/dl)
Group I
24.00 3.46
9.00 2.00
232.63 36.52
79.40 0.87
5.15 0.84
71.82 14.04
HDL(mg/dl)
30.50 0.16
LDL(mg/dl)
25.44 0.05
Group II
31.88 3.47
12.25 2.60
519.47 95.01
92.10 2.72
9.74 0.16
64.17 8.93
35.800.08
9.950.04
Group III
27.38 7.82
9.63 1.11
424.58 68.78
84.85 1.06
8.63 0.32
64.60 8.80
35.60 0.16
12.03 0.01
Results represent mean standard deviation (n=4). Values in the same roll having the same alphabet are not
significantly different (P>0.05)
Legend: Group I = Control (Fed normal rat chow)
Group II = Fed 60% sample and 40% normal rat chow
Group III =Fed 30% sample and 70% normal rat chow
Group II
a
23.503.79
a
11.132.25
a
306.3274.91
a
81.292.27
a
6.731.27
a
101.6516.47
a
45.001.63
a
40.310.09
Group III
b
36.754.97
b
14.503.67
b
445.1046.69
b
107.331.41
b
11.390.61
b
85.5312.60
b
48.50 0.16
b
15.56 0.36
33.134.87
a
14.130.75
c
386.0827.78
97.564.08c
c
9.660.70
c
93.2113.29
a
46.00 1.63
c
27.70 0.24
Result represent mean standard deviation (n=4). Values in the same roll
having the same alphabet are not significantly different (P>0.05)
Legend:
Group I = Control (Fed normal rat chow)
Group II = Fed 60% sample and 40% normal rat chow
Group III =Fed 30% sample and 70% normal rat chow
Serum lipid profile and liver enzymes of Rats fed Lageneria sphaerica (Wild bottle gourd) supplemented diet
031
Table 4. Serum lipid profile and selected liver enzymes activities of male albino rats fed Lageneria sphaerica supplemented
diet after 49 days
Group I
AST (I/u)
ALT (I/u)
ALP (I/u)
TG (mg/dl)
TP (g/dl)
TC (mg/dl)
HDL (mg/dl)
LDL (mg/dl)
Group II
a
26.635.71
a
12.252.60
a
319.3883.15
a
93.648.58
a
7.501.82
a
109.7113.29
a
45.500.16
a
45.480.02
Group III
b
37.383.42
b
17.882.25
b
483.8662.09
b
121.711.70
b
13.231.90
b
89.039.72
b
48.800.08
b
15.890.09
32.504.53
c
16.754.50
a
398.948.51
c
112.135.54
c
10.240.41
a
93.496.68
b
48.210.06
c
22.860.11
Result represent mean standard deviation (n=4). Values in the same roll having the same alphabet are not significantly different
(P>0.05)
Legend:
Group I = Control (Fed normal rat chow)
Group II =Fed 60% sample and 40% normal rat chow
Group III = Fed 30% sample and 70% normal rat chow
Figure 1. Histological picture of liver of rats in group I (Control) showing normal liver morphology
Serum lipid profile and liver enzymes of Rats fed Lageneria sphaerica (Wild bottle gourd) supplemented diet
Chinyere et al.
032
Central vein
Figure 2. Histological section showing enlarged central vein of group II rats
Hepatocytes
Figure 3. Histological section of group III rats showing liver tissue with distorted interstitial (stroma) and structural hepatocytes (atrophy) after the
feeding period. Observe an enlarged central vein with evidences of degeneration within the stroma The laminae and portal triads are not distinct.
Serum lipid profile and liver enzymes of Rats fed Lageneria sphaerica (Wild bottle gourd) supplemented diet
033
Central vein
Figure 4. Shows the central vein of group II rats liver after the feeding period. Central vein enlarged.
Serum lipid profile and liver enzymes of Rats fed Lageneria sphaerica (Wild bottle gourd) supplemented diet
Chinyere et al.
034
Hepatocytes
Portal tract
Fig VI shows group I (control) rat liver .Histological picture indicating normal hepatocytes and portal tract.
Serum lipid profile and liver enzymes of Rats fed Lageneria sphaerica (Wild bottle gourd) supplemented diet
Hepatocytes
035
Figure 7. Histological section of the group III rat liver showing liver tissue with distorted interstitial (stroma)
and structure hepatocytes (atrophy). There is an enlarged central vein (cv) with evidence of degeneration
within the stroma. The lamina and portal triads are not distinct.
REFERENCES
Akiyama T. Almo SC, Smith DL, Danishefsky AT and
Ringe D (1997).The structural basis for the altered
substrate specificity of the R292D active site mutant of
aspartate aminotransferase from E. coli. Protein
England Journal of Medicine 7 (3): 405412.
Anaga AO, Njoku CJ, Ekejiuba ES, Esiaka MN, Asuzu IU
(2011). Investigation of the methanolic leaf extract of
Costus afer Ker for Pharmacological activities in vitro
and in vivo. Phytomedicine.11 (2-3): 242-248.
Bassey O.A, Lowery, O.H, and Brook M. (1946). One
point colometric method of determing alkaline
phosphatase in serum or plasma. Journal of Biological
Chemistry. 164:321.
Brunt, P.W., Losowskey, M.S. and Read, A.E. (1984). The
liver and biliary system, William Heinemann, Medical
Books Ltd London, Pp1357
Burtis, C.A and Ashwood, F.R. (1996). The fundamentals of
clicnical chemistry. Saunders philadephia P.A. Pp 299327.
Chinyere G.C ., Akubugwo E.I., Nwaukwa N.I and
Ugbogu A.E (2009). Nutritional value of Lagenaria
Serum lipid profile and liver enzymes of Rats fed Lageneria sphaerica (Wild bottle gourd) supplemented diet
Chinyere et al.
036
Copyright: 2015 Chinyere et al. This is an openaccess article distributed under the terms of the Creative
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provided the original author and source are cited.
Serum lipid profile and liver enzymes of Rats fed Lageneria sphaerica (Wild bottle gourd) supplemented diet