120:360 - Biochemistry

Fall 2015
Sample questions for Exam 2

1)

Which of the following is a diastereoisomer of D-glucose?

a. D-galactose
b. D-lactose
know the definition + sugar structures
c. D-fructose
d. L-glucose
e. none of the above

2) Which of the following contains a glycosidic linkage?

a. glucose
b. fructose
c. ribose
d. -D-glucopyranose
e. lactose
3)

A net change which occurs during glycolysis is the

a. synthesis of glucose-6-phosphate
b. conversion of NAD+ to NADH
c. synthesis of phosphoenolpyruvate
d. conversion of ATP to ADP + Pi
e. all of the above

4)

The activity of chymotrypsin would be expected to be decreased at low pH

because of the loss of the negative charge on the R group of
a. phenylalanine
b. aspartate
know charge of AAs + what chymotrypsin is
c. serine
d. alanine
e. all of the above

5)

When a reversible reaction is at equilibrium, ________ is equal to zero.

a. Keq
b. G
c. Go
d. Go
e. none of the above

6)

A compound which contains a phosphoric acid-carboxylic acid anhydride is

a. fructose6phosphate
b. fructose1,6bis-phosphate
c. glyceraldehyde3phosphate
know glycolysis structures
d. 1,3bisphosphoglycerate
e. all of the above
1

7)

The following graph shows the results obtained when the rate of an enzymecatalyzed reaction is measured as a function of substrate concentration.

From these results we can conclude that the enzyme probably

a. functions by a ping-pong mechanism
b. follows classical Michaelis-Menten kinetics
c. has multiple substrate binding sites
d. is a serine protease
e. is inhibited by CTP
8)

During catalysis by serine proteases such as chymotrypsin the first step involves a
nucleophilic attack by
a. a carbonyl group
b. a hydroxyl group
know catalysis of serine proteases. read.
c. a carboxyl group
d. an imidizole (histidine R) group
e. H2O

9)

The first enzyme in glycolysis (hexokinase) catalyzes the

a. addition of inorganic phosphate to glucose
b. conversion of ATP to ADP and inorganic phosphate
c. transfer of a phosphate from ATP to glucose
d. both a and b
e. none of the above

Study what hexokinase does

10) The large negative free energy of hydrolysis of phosphoenolpyruvate results mainly
from its restricted
a. rotation around its peptide bond
b. stereochemistry
Study glycolysis structures and concepts
c. tautomerization to the keto form
d. resonance
e. entropy

11) A bisubstrate reaction consists of:

S1 + S2

P1 + P2

In bisubstrate reactions which proceed via a ping pong (double displacement)

mechanism, a modified enzyme (E) is temporarily formed. What is E in the
chymotrypsin reaction?
a. Enzyme covalently linked to one of the products.
b. Enzyme covalently linked to both of the products.
Study chymotrypsin
c. acyl-enzyme intermediate
d. both a and c
e. both b and c
12) The rate (v) of an enzyme reaction S
P is measured as a function of [S].
The same experiment is then carried out with a higher concentration of enzyme.
Which of the following will most likely be different in the second experiment?
a. Km
b. Keq
c. Vmax
d. both a and b
e. both a and c
13) In the Michaelis-Menten model for an enzyme-catalyzed reaction:
k1
E+S

ES

k2

EP

k3

E+P

k-1
the parameter which is a function of the energy of activation for the rate limiting step is
a. k1
b. k-1
c. k2
d. k3
e. None of the above
14) In the Michaelis-Menten model (see previous question) the equation
k1[E]free[S] = k-1[ES] + k2[ES]
is a mathematical expression of
a. the extent of catalysis (i.e. rate acceleration) by the enzyme
b. the Keq for the reaction S P in the presence of the enzyme
c. competitive inhibition
d. non-competitive inhibition
know what the EQ is for
e. the steady state assumption
know what SS assumption is

15) The following equation

Vmax[S]

v=

_________________________

(1+[I]/KI)(Km) + [S]
describes the situation in the presence of a(n) ___________ inhibitor
a. allosteric
b. competitive
c. non-competitive
d. non-specific
e. metabolic (hormonal)
16) Identify the following compound:

a.
b.
c.
d.
e.

Phosphoenolpyruvate
Glyceraldehyde-3-phosphate
3-phosphoglycerate
2-phosphoglycerate
Dihydroxyacetone phosphate

know glycolysis structures

17) The following are graphs of v vs. [S] for phosphofructokinase-1 in the absence or
presence of ADP.

These graphs indicate that ADP is

a. a competitive inhibitor of the enzyme
F1,6P = Allos. activator of Pyruv. kin.
ADP = prohibits the inhib of PFK by ATP
b. an non-competitive inhibitor of the enzyme
c. an allosteric inhibitor of the enzyme
d. an allosteric activator of the enzyme
e. able to overcome the inhibitory effect of ATP on the enzyme

18) In the following structure of ATP which of the bonds represent(s) an anhydride
linkage?

a.
b.
c.
d.
e.

1
2
3
Both 1 and 2
Both 2 and 3

19) You have carried out an experiment in which you incubated 14C-labelled glucose
with a liver extract. The only radioactive compound you observe produced is
14
pyruvate with C in its methyl carbon. Which carbons of the glucose you started
14
with could have contained the C (where carbon #1 is the aldehyde)?
a. 1 and 6
b. 2 and 5
as you study glycolysis structures, keep track of carbon numbers
c. 3 and 4
and see where they end up
d. 1,2 and 3
e. 4,5 and 6
20) Picture A shows the enzyme-substrate complex of a serine protease. Picture B
shows how hydrogen bonding stabilizes the

a.
b.
c.
d.
e.

substrate
product
transition state
enzyme
both a and b

Study the ppt

on serine protease. read book.