2011

100003

2008 2009 2010

2010 ISEF
ISEF

ii

iii

2 l

iv

Study of the automatic injection system for silkworm

in engineered protein production

Abstract
This research aimed at developing an automatic injection system for silkworm to
produce silk with specified antimicrobial peptides. Silkworm was a good carrier to
produce good-quality engineered protein cheaply and efficiently. And an automatic
injection system could significantly increase the production efficiency. In this research,
the silkworm skin was tested to find the optimal injecting location. Then a controllable
silkworm-fixing device which applied electro-rheological fluid was designed. Besides,
image recognition was also applied to perform the dual-axis locating. A circular
conveyer system transported silkworm to be injected by a designed micro injector. At
the last part the concentration of baculovirus was controlled to make silkworm
producing a cocoon which contained specified antimicrobial peptides. Bandages made
of such silk could be helpful to prevent wound being infecting.


iv
1
2
6
7
7
16
--- 30
47
53
54

vi

[1,2]

1.
2.

3.
4.
5.
6.
7.

1.
2.
3.
4.
5.

1.
2.
3.

1.
2.
3.
4.
5.
6.

1.
2.
2.1
2.2
2-3
3.
4.
5.
6.

--

1.

2.

3V NXT NXTCam
NXTCamView NXT 9V
Robolab 2.9NXTG 2.0

1.
2.
3.

1.
4

163 [2] 1.1 [3]

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1.1 [3]
7

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1 411 [3]

212
2.

[1]
1.2

1.2

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a.

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c.
d.

(1)
(2)
(3)
3.

(1) 24

(2)
(3)

(1) ( 1.3) 1.4

(2)
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(1)
2
(2)
(3)
10

(1) C 10
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(2) ( 1.6) 1.7

(3)

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1.6

1.7

11

(1) 4% Paraformaldehyde/0.1M PBS ( 1.8) 4

(2) 15% Sucrose/0.1M PBS 4
(3) 30% Sucrose/0.1M PBS 4
(4) OCT
(5) 8 1 cm3-20(
1.9) 7~10 m
(6)

1.8

1.9

(1) 1.10
(2)
12

 1.11
(3) 1.12 1.13
(4) 1.12 1.13

(5) 1.12

(6) 3mm
3mm

1.10

1.11

1.12

1.13

13


(1)

(2) 3mm
3mm
4.

1.14
0.7mm

1.14
14


(1)
(2)
(3) 5~11

(4)

(1) 1.15
(2) 1.16 1.17
(3) 1.18

(1) 1.18
(a)
(b)
(c)
(2) [3]

(3) 8

15

1.15

1.16

1.17

5 6 7 8 9 10 11

1.18

(1) 10 m

(2) 8~11 1/3

2~3 mm

5l

16

 60

(1)
(2)
(3)
(4)

2.1

(1) 35 cm 6 cm

17

(2) LEGO 40 cm
(3) 9V
7

(1)
1 cm

(2) 2A.1
10 cm /sec

2A.1

(1)

18

(2)

(3)

2.1

2.1

(1) PE 6 cm x 2.5 cm
2B.1
(2) 12 cm 3.5 cm x 3 cm
2.5 cm

19

(3) 1.5 cm

2B.1

2B.2

2B.3

(1) 2B.2
20

(2) 30
( 2B.3a)( 2B.3b)
( 2B.3b)
(3)
(4) 2B.3a
2B.3b
C

8~11
1/3 2~3 mm

(1)
12 cm
12 cm

LEGO LEGO

(2)

21

2.2

2.2

22

2.3

2.3

(1) 3V 3V
2C.1
(2)
2C.2
(3)
(4) NXT

23

2C.1

2C.2

(1) 2C.2
(2)

(1)
(2) 2mm
(3) 5l

24

(1)
( 2D.1) 1 mm
(2) LEGO
24:1
3.1 mm( 2D.2)

2D.1

2D.2

(3)
(4)
1 mm ( 2D.3)
1 mm
2 mm
(5) 5l
24:1(
2D.4)

25

3.1 mm 24

0.5 ml 280 mm

0.129

mm
3.1
0.129
24
rev
0.5 ml
l

17.86
28 mm
mm

l
l
mm
17.86
2.3
rev
mm
rev

2.3l 2.174 ( 2
60 ) 5l

2D.3

2D.4

(1) 2D.1
(2)
(3)

(4) 1 mm

(1) 2D.2 2D.4

26

(2) 2 mm 780
5 l
E

(1) 10 mm 15 mm
(2)
(3) 5mm

(1) 2D.5
(2)
5 mm

(3)

27

2D.5

F
2F.1 Robolab 2.9
2F.2 NXT NXT 4
3 2.4

2F.1

2F.2 Robolab

28

 2.4

3, 4

(1) ( 2G..1)

(2)
30 2~3
(3)
(4)

(5) 40 cm x 24 cm x 24 cm

29

2G..1

---

(1)

(2)

(3)
(4)

(5)

30

(6) 0.7mm

(7)
3

10

(1) 120 mm
(2) LEGO 1 9V
21

(3) 3 120

31

(1) 3A.1
21 3 sec / rev
(2)

(3) 14 cm x 12 cm x 6 cm
75%

3A.1

B
B.1
B

(1)

32

(2)
(3)

3B.1
3B.2

3B.1

3B.2

(1) 50 mm 25 mm 1.5 cm

(2) 20 mm 15 mm
(3)

33

(4)

(1) 3B.3
(2)

(3)
3B.4

(4)

3B.3

3B.4

B.2

(1)

34

(2)

(1)

(2)

()

( 10 ms)

35

(Electro-rheological Fluid)
(1~10 m)

[5]
3.1

3.1

B-3

(1)
(2) (
36

photo)
(3)

(1)

[5]

(1)
0.8 mm
(2) 200 cs
15%20%25%30%35%
(3) 1 c.c.

(4)

(1) 600V 1kV

(2) 3.2
(3) 35%
37

 3.2

15%

20%

25%

30%

35%

(mm)

0.8

0.8

0.8

0.8

0.8

(mm)

1.6

2.5

3.3

3.9

4.5

(2)

()

( 3.3)
()

3.3

PE

38

(
)

(1) 2 ()
( 3B.5)
(2) PE U

(3)
(4) U
(5)

(1) 3B.6 U
(2)
90

39

 3B.5

3B.6 U

B
(1)

(2)

(3)
(4)

40


NXT
NXTCam NXTCamView
NXTCam NXTCamView Mindsensors LEGO NXT
176x144
88x144 NXT

NXTCamView RGB
RGB
3C.1 NXTCam 3C.2 NXTCamView

3C.1 NXTCam

3C.2 NXTCamView

(1)
(2)
1

41

 1 411

9
8~11
120
120 NXTCam

(1) NXTCamView
(2)
(3) NXTCam NXT NXT-G
NXTCamView
(4) NXT-G
(5) NXT-G

(1) 3C.3 NXTCamView 3C.4

NXTCamView Colormap 3C.5

(2) NXTCam
LED

42

(3)

3C.3

3C.4 colormap 3C.5

(1)

(2) 0.7mm
(3)
3

43

(1)
(2) 0.21 mm
50 mm

10 mm ()

(3)
2
1/3
3D.1 3D.2 3D.3
1/2

3D.1

3D.2 0.21 mm

3D.3

44

F
NXTCam NXTCamView
Robolab LEGO NXT-G
3F.1

3F.1 NXT-G

3G.1 3G.2

(1)

(2)

45

 3G..1

3G.2

(1) 3H.1
(2) 15 cm x 27 cm x 10 cm 25%

(3) 20
4

46

OK

(1)
(2)

3~4 ( 4.1)

( 4.2)
105 pfu/5ml AcMNPV

AcMNPV

47

 4.1 [1]

4.2 1]

(1) AcMNPV :106 pfu/5ml 105 pfu/5ml 104 pfu/5ml

12 12

(2) 4
(3)

(1) 4.1
(2) ( 4.1)

OJ01 OJ03

48


AcMNPV :106 pfu/5ml107 pfu/5ml108 pfu/5ml109 pfu/5ml1010
pfu/5ml 8
8

(1) 4.2
(2) AcMNPV

(1) 104~1010 AcMNPV

(2) OJ01
OJ03 OJ01 OJ03
AcMNPV

OJ01 OJ03
OJ01 OJ03 20
8
3 AcMNPV :105 pfu/5ml104 pfu/5ml103 pfu/5ml
2 2

49

(1) 3 4.3
(2) 4.3 OJ01 OJ03

(3) 105 pfu/5ml 3

104 103 pfu/5ml 5
5

(1) 104 103 pfu/5ml AcMNPV OJ01 OJ03

(2) 5

(3)

4.1 AcMNPV
(10n pfu/5ml)

n=4

n=5

n=6

12

12

12

12

50

 4.2 AcMNPV
(10n pfu/5ml)

n=6

n=7

n=8

n=9

n = 10

4.3 AcMNPV
(10n pfu/5ml)

n=3

n=4

n=5

4.1

103 pfu/5ml

4.2

104 pfu/5ml

4.3 OJ01 OJ03

51

 AcMNPV

[6,7,8]
(1) Royalisin
(2) Pleurocidin
(3) Monodocin

(1) Royalisin
Royalisin
(2)
Royalisin
(3)
(4) OK

52

2~3

25%

OJ01 OJ03
104 103 pfu/5ml AcMNPV

53


1. 2008
2. pp.282008.11
3. http://www.silkworm.org.tw/silkworm/tsdf3-2.asp
4. 48 2008
5. 24
2007
6. Royalisin8:43-502006
7. pleurocidinNo.15pp.34-40
2008
8. (Baculovirus)
e 63 2006

54

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