Total number of hours (per semester) from

the educational plan

(55 )

THE SHEET OF DISCIPLINE

Name of the department

Year of study

BACTERIOLOGY-VIROLOGY
II

Semester *

Type of final evaluation (E / V / C)

Status disciplinei (Ob madatory, Op optional, F - facultative)

Ob Credits number

Total hours from the educational

plan

25

Head of Departament

55

Total hours of individual study

Total hous per

semester

Prof. Univ. Dr. Luminita Smaranda Iancu

* Dac disciplina are mai multe semestre de studiu, se completeaz cte o fi pentru
fiecare semestru
Faculty

Medicine
Total

Department

C**

Medicale II
55

Profile

27

28

Medicine

Specialization
** C lectures, L labs, P practical activities, projects
Knowledges prior to Microbiology courses: biology, biochemistry,
physiology, biophysics, immunology, genetics
Objectives of the discipline
The main objectiv of Medical Microbiology is to teach the future medical
doctor to see the microorganisms with the eyes of the mind and to judge
appropiately every medical issue:
-A. To aknowledge the diversity of microorganisms on/ in human body (both
healthy and sick individuals) as well as in the environement.
-B. To know pathways of microorganisms spreading and the consequences
of contamination.
-C. To aknowledge the risk of contamination in medical settings.
-D. To understand the role of microorganisms related to health and diseases
condition.
Lecture Analytical Curricula
Hours
1. Microbiology, microbial world, microbiology disciplines.
1

E2
6
80

2. Bacterial morphology and functions. Classification of structures.

Structures important from a medical point of view and their
biological, taxonomic and pathogenic significance: genome, bacterial
wall, glycocalyx, flagella, axial fiber, bacterial endospore
3. Basic principles of nutrition, metabolism and bacterial cutivation and
growth.
4. Microbial genetics (the role of bacterial genetics in pathogenicitz of
infections and bacterial strains evolution, and the role of genetics in
microbiological diagnosis)
5. Relations microorganism-host. Human indigenous microbiota.
Microbial pathogenicity. Anti-infective defence.
6. Infection (definition, sources, transmission, susceptible hosts).
7. Microbiological bases for infection prophylaxis and therapy
(definition, classification, their mechanism of action and activity
spectrum, basic principles of infections prophylaxis, with indication
and contraindication).
For the main genus implicated in human patholohy will be teach: brief
definition, microscopical and cultivation particularities, resistance to
environment factors, pathogenicity factors, list of the main diseases,
microbiological diagnosis, basic principles regarding treatment and
prevention.
8. Staphylococcus genus.
9. Streptococcus and Enterococcus genus.
10. Enterobacteriaceae family.
11. Vibrio, Campylobacter and Helicobacter. genus. Pseudomonades of
medical interes. Acinetobacter genus.
12. Gram negative cocobacilli. Neisseria and Moraxella genus.
Laboratories Analytical Curricula
1. Asessing the ubiquity of microorganisms in the environment. Washing,
disinfection, antiseptization.
2. Sterilization by moist heat, dry heat. Quality control.
3. Bacterial cultivation: culture media, pure culture techniques, culture
characteristics, techinque of a smear from bacterial culture.
4. Microscopy: microscopical preparations, differential stains, oil immersion
microscopy, identifications of microscopic categories
5. Laboratory diagnosis of infection. Antigen-antibody reaction in
microbiology laboratory.
6. Antibiogram (diffusimetric method)
7. Antibiogram (dilution method)
8. Blood culture in laboratory diagnosis of infection. Identification of
coagulase negative staphyilococci and alpfa hemolytic steptococci . MCQ
general microbiology
9. Laboratory diagnosis of pus. Identification of coagulase positive
staphyilococci.

2
1

2
1
4

3
4
2
2
2
2
2

2
2
2
2

10. The exam of the pharynx exsudate. Identification of beta hemolitic

streptococci.
11. The cyto bacteriologic examination of the bronchial alveolar exsudate.
Identification of pneumococci.
12.Laboratory examination of the wound and plagues. Identification of
Pseudomonas.
13.Etiological investigation of meningitis. Identification of meningococci.
14.Cytobacteriological examination of urine. Identification of Gram
negative aerobic /facultative anaerobic bacilli.
Competences
The students must be able to:
1. Prevent microbial contamination in specific conditions from medical
and surgical practice.
2. Collection, transport and storage of biological samples for
microbiological investigation.
3. To make a smear for microscopic examination of bacteria.
4. To perform and to interpretate Gram and Ziehl Neelsen stainings.
5. To identify the main microorganisms involved in human infections.
6. To understand the result received from the laboratory (serological
diagnosis, bacterial susceptibility testing).
7. To understand the clinical significance of a certain isolate.

For the final mark will be taken into account

2
2
2
2

Part of final mark in percentage

(Total = 100%)
50%

-answers from the final MCQs

-answers from the practical exam

35%

-marks from the tests during the semester

15%

-continous testing during the semester

-activities such as essays/ translations/projects
- other activities
The final evaluation will be
Written exam MCQs

Practical and oral exam

Total hours per semester required for individual study of the student
1. Understanding the lecture notes

8. Preparing for oral presentations

9. Preparing final examination

10. Consulting

11. Field research

10

12. Internet research

6. Homework, translations, essay

13. Other activities...

7. Preparing for test

14. Other activities...

2. Study according to manual,

lectures
3. Study according to indicated
bibliography
4. Additional documentation in the
library
5. Specific activity in order to
prepare for labs

TOTAL hours of individual study per semester

(minimum) = 25

Compulsory bibliography
1. Lectures power point presentations
2. Laboratories protocols
3. Luca Mariana. Parazitologie.Micologie. Editura UMF. Iai, 2005.
Supplementary bibliography for Microbiology lectures and laboratories
1. Jawetz, Melnick and Adelbergs Medical Microbiology, Appleton & Lange, 2007.
2. Ferron A Bacteriologie medicale, Editions C. et R., Paris, oricare editie de dupa
1995.
3. Mims CA et al.: Medical Microbiology, Mosby Company, St. Louis, 2007.
4. Murray PR et al.: Medical Microbiology, Second Edition, Wolfe International
Student Edition, 2005.
5. Luca Mariana. Parazitologie.Micologie. Editura Medical. Bucureti, 1993.
6. Radulescu Simona. Parazitologie medical. Editura All Educational, Bucureti, 2000.
7. Parijia S. C., Textbook of Medical Parasitology, 1st ed., All India Publishers, 1997

Date:

Signature of Disciplines coordinator