Introduction

Part I: As an illustration, food microbiology is one of the microbial fields that requires the use of
growth curve. (Huang - author last name) describes it as significant tool for risk analysis and
shelf life prediction to analyze growth or inactivation of microorganisms in food.
Part II: Growth curve also plays a significant role in studying the growth and division of
microorganisms. (Ilyer-Biswas et. al) report that this helps to yield conclusions for different
biological processes such as the development of organisms, the growth of tumors, and
pathogen-host interactions.
(Part III): Dilution plate counts could have both advantages and disadvantages to itself. (Khalid)
shares that it is an advantageous method, because it measures the number of viable cells.
Part IV: Similarly, dilution plate counts is a labor intensive method to quantify the bacterial
growth. According to professor Khalid from the University of Khartoum, researchers must
be careful with their technique to prevent developing an excessive amount of colonies, because
the cells would not be able to grow, which increases the level of inaccuracy of the
measurement.
(Part V): Similar to the dilution plate counts method, this method also has its own advantages
and disadvantages for the users. In terms of its advantages (Durai Arun P - the paper did not
provide authors last name) reports that it is very efficient method that allow researchers to
accurately measure reflectance, absorbance, optical density of reaction mixtures. On the other
hand, the disadvantages of the 96 Well Plate method also exists. (Durai Arun P) reports that as
the system architecture of the microtiter plate readers evolves over time, it requires changes in
both hardware and software of the computer to ensure compatibility.
Introduction:
Growth curve has been a common graphical representation of bacterial growth and
enumeration process.(It is very important in different biology-related fields) (Part I, Part
II)This curve plots the incubation time against the log number of cell growth to outline the four
main phases of bacterial growth: Lag Phase, Exponential Phase, Stationary Phase, and Death
Phase. (Vlab) Lag phase happen at the beginning of the growth curve where no significant cell
growth would be observed. At this stage, bacterial cellular material would still be recovering from
the aseptic transfer and adjusting to the environment. Exponential phase or the Log Phase was
termed for the duplication of the number of cells in a regular time interval. This phase was used
extensively to determine that the batch culture was alive and to determine generation time.
Techniques to determine growth curve can varies. A classical method used was Dilution
Plate Count/Side Arm Flask, colonies growth collected on Tryptic Soy Agar (TSA) can be
taken as a measure of the viability of individual cells because growth indicate that the
bacterial population was alive and reproducing. ( - rephrase?) (Part III) (Jenningson)
Often times, plate counts can be time consuming and the results can be lower than the actual
counts due to (either) clumping or death of bacteria due to(because of the) unsuitable
environment of the plates. (Part IV) One of the more modern techniques (that was) introduced
for viable cell count was (the) 96 Well Plate using a microtiter plate readers to digitally measure
the Optical Density of a large amount of culture at one time. This method significantly
increased accuracy of the counts and would be more time consuming. (should be
removed) (Part V)