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DOI 10.1007/s11051-015-3004-7
RESEARCH PAPER
G. D. Mogosanu
Department of Pharmacognosy & Phytotherapy, Faculty
of Pharmacy, University of Medicine and Pharmacy of
Craiova, 2 Petru Rares Street, 200349 Craiova, Romania
R. Trusca
Metav SA-CD S.A., 31 Rosetti Street, 020015 Bucharest,
Romania
F. Iordache
Department of Fetal and Adult Stem Cell Therapy,
Institute of Cellular Biology and Pathology of Romanian
Academy, Nicolae Simionescu, 8, B.P. Hasdeu, 050568
Bucharest, Romania
M.-C. Chifiriuc A. M. Holban
Microbiology Department, Faculty of Biology, University
of Bucharest, Aleea Portocalelor No 1-3, 060101
Bucharest, Romania
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Introduction
Staphylococcus aureus is the most versatile and well
studied Gram-positive bacteria, causing severe infections in hospitalized patients, especially in intensive
care units and in patients bearing prosthetic devices
(Yan et al. 2013; Holban et al. 2013). S. aureus also
exhibits high resistance to many physical, chemical,
and biological agents (Yan et al. 2013; Holban et al.
2013). Methicillin-resistant S. aureus strains are resistant not only to penicillins and cephalosporins, but also
to other classes of anti-staphylococcal antibiotics
(fluoroquinolones, aminoglycosides), being responsible for staphylococcal infections with severe evolution
and high mortality rates (Tacconelli et al. 2008).
On the other hand, the opportunistic pathogen
Pseudomonas aeruginosa is one of the most lifethreatening Gram-negative bacteria, particularly in
immunocompromised and cystic fibrosis patients
(Holban et al. 2013). P. aeruginosa can easily adapt
to any environment, mainly due to its ability to form
biofilms on different industrial equipments and
medical surfaces, causing major health-related and
economical issues (Kim et al. 2013). P. aeruginosa is
naturally resistant to the majority of currently used
drugs and may also acquire resistance during or after
antibiotic treatment (Poole 2004).
In the recent years, finding new or alternative
treatments to cure dangerous, drug-resistant infections,
became one of the top priorities of the biomedical
research (Tacconelli et al. 2008). Developing antimicrobial therapies based on natural compounds
represents a desirable strategy to fight resistant infections, to decrease the rate of selecting resistant mutants
and also to reduce the side effects associated with the
use of synthetic drugs (Saviuc et al. 2013; Grumezescu
2013; Grumezescu et al. 2012; Chifiriuc et al. 2012;
Grumezescu et al. 2012; Saviuc et al. 2012; Anghel
et al. 2013; Anghel et al. 2013; Istrate et al. 2014).
M.-C. Chifiriuc
Life, Environment and Earth Sciences Section, Research
Institute of the University of Bucharest-ICUB, Spl.
Independentei 91-95, Bucharest, Romania
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Fig. 2 a N2 adsorptiondesorption isotherm of prepared NMS, b XRD pattern of NMS, c TG analysis of NMS, NMS/SO, and NMS/
CS
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Conclusions
Fig. 5 Fluorescence microscopy images of viable endothelial cells stained with RED CMTPX fluorophore, grown in the presence of
tested bio-active NMS (concentration of 1 mg/mL) versus control (endothelial cells grown in standard conditions)
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Fig. 9 Graphic
representation of the effect
of different concentrations
of NMS/SO, NMS/CS,
NMS/STR, and NMS/NEO
on the viability of S. aureus
(a) and P. aeruginosa
(b) tested strains
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