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Alkhedhairy et al.
(54)
(75)
Publication Classi?cation
(51)
(SA)
(73) Assignee:
(21) Appl. No.:
(22)
Filed:
(52)
Int. Cl.
A01N 25/08
A01P 1/00
A01N 59/16
C22B 11/00
C22C 5/06
(2006.01)
(2006.01)
(2006.01)
(2006.01)
(2006.01)
(SA)
(57)
12/776,425
ABSTRACT
US 2011/0274736 A1
100
102
tryptophan)
l
Maintain the Solution at 28C Under Constant
Agitation
106
108
110
and Filtering
l
Acidify the Recovered Supernatant To Achieve A pH \
of 2.0 Using HCl
112
l
Dissolve the PCA Crystals In Deionized Water
116
l
x{Filter the Solution (PCA Crystals + Deionized Water))
118
Fig. 1
US 2011/0274736 A1
[
202
200
204
206 \
.
l
.
Nanoparticles In A Region To
Inhibit Growth of
.
/- 20a
Fig. 2
Growth of MicroOrganisms In
?bswame
Wavehangth {Wm}
US 2011/0274736 A1
US 2011/0274736 A1
w
E,
N
ism
55
IE @
Fig_ 4
$3
hiawmn
am:
am
Waw?angth {mm}
US 2011/0274736 A1
US 2011/0274736 A1
{m} -
3%
US 2011/0274736 A1
:n
363%
max}
553;;
mm
US 2011/0274736 A1
{m
g;
a?
a;
be:
ii
'
333%:
493%}
w?
mi
?imiaimgih {mm}
Fig. 8
US 2011/0274736 A1
BACKGROUND
[0004]
[0013]
[0017]
DETAILED DESCRIPTION
OvervieW
[0014] The methods described herein relate to the produc
tion of silver nanoparticles for use in antimicrobial and other
US 2011/0274736 A1
[0024]
[0026]
over time.
10 nm to 38 nm (nanometers).
[0021]
[0022]
US 2011/0274736 A1
tion Was relatively fast during the ?rst tWo hours, then gradu
ally sloWed and attained equilibrium after 72 hours.
[0036] The redox potential of the PCA Was assessed using
[0034]
constant stirring.
[0038] The color of the aqueous medium changed from
pale yelloW to reddish-broWn after tWo hours, and the color
intensity increased for a time period up to 72 hours. At regular
time points, aliquots of 1 ml Were collected and monitored for
Surface Plasmon Resonance of silver nanoparticles. After 48
hours, the reddish-broWn aqueous solution Was freeZe-dried,
and the resulting poWder of silver nanoparticles Was stored at
comprising:
preparing a liquid solution containing phenaZine-1-car
boxylic acid; and
US 2011/0274736 A1
bating the combined silver metal salt and liquid solution for
approximately tWo hours at approximately 28 C.
3. A method as recited in claim 1 further comprising incu
method comprising:
preparing a liquid solution containing a redox-active
phenaZine metabolite;
adding silver metal salt to the liquid solution; and
incubating the liquid solution at an ambient temperature to
salt is AgNO3.
nanoparticles.
9. A method as recited in claim 1 Wherein the method is
performed at an ambient temperature.
10. A method as recited in claim 1 Wherein the silver
silver nanoparticles.
19. Silver nanoparticles produced by the method of claim
12.
boxylic acid;
adding silver metal salt to the liquid solution to produce a
that region.
21. A method as recited in claim 20 Wherein distributing at