Professional Documents
Culture Documents
Research Design
This descriptive study is designed to assess the microbial
population of microorganisms in street-vended food stuff from
selected mobile food stall in West Visayas State University La
Paz, Iloilo City. This research study was performed in West
Visayas State University, Research Laboratory.
Materials
The experimental materials are food samples from a selected
food stall in West Visayas State University La Paz, Iloilo City,
Mannitol Salt Agar, Nutrient Agar, E. Coli Broth and distilled
water.
The glassware include petri dishes, graduated cylinder,
Erlenmeyer flask, beakers, culture tubes, glass jar,and Pasteur
pipettes.
Autoclave, centrifuge, analytical balance, and
electric water bath and other equipments are classified
under the non-glassware materials.
Methodology
Sterilization of Materials. The researcher washed the glass
wares dry and wrapped them with an old cloth or newspaper. Then,
they were placed inside the autoclave at 15 psi for 15-20 minutes
at 121C. After sterilization, the researchers dried them.
Afterwards, the materials were stored for later use.
Sample Collection. Only one vending site was considered.
Fishballs (cooked) prepared and/or sold by vendors were collected
for microbial analysis.
Five samples of about 10 g of each food on sale were placed
in sterile ziplock plastics adequately labeled and transported to
the laboratory for analysis within two-three hours of collection.
several culture tubes with durham tubes. This was allowed to cool
and store for later use.