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CHINA VEGETABLES
100097
063002
3 480.9 molkg-1
FW 318.8 molkg-1FW 213.9 molkg-1
FW 3 44 % 5 4-
-3-4--3-4--3-
-3- 4--3-6 4-
-3- 70.5 %87.5 %
HPLC
S631.1
1000-6346201018-0043-04
1Vegetable Research Center, Beijing Municipal Academy of Agriculture and Forestry, Beijing 100097, China;
2
the highest total glucosinolate content 480.9 molkg FW, followed byJingyanshuiguoxingluobo
-1
-1
318.8 molkg FW. The lowest concent is found inMantanghong213.9 molkg FW,
taking 44 % ofJinghong No.3content. After analyzing glucosinolates composition, we find 5 types of
radish glucosinolates, including 4-methylsulfinyl-3-butenyl-glucosinolates, 4-hydroxyindol-3methyl-glucosinolates, 4-methylthio-3-butenyl-glucosinolates, indol-3-methyl-glucosinolates and
4-methoxyindol-3-methyl-glucosinolates. The predominant glucosinolate in 6 radish varieties is
4-methylsulfinyl-3-butenyl-glucosinolate representing, taking about 70.5 %-87.5 % of the total
amount.
2010-05-102010-07-19
2008BADB1B03
E-mailzhangli@nercv.org
www.cnveg.org
44
CHINA VEGETABLES
2010 9
glucosinolatesGSL
R 3
myrosinaseisothiocyanates
Mithen2001
80 Mithen et al.2003Sarikamis et
al.2006Raphanus sativus L.
2 000
1.1
6 2 3
3 4 1 1 3
4 1
2009 8 7
2
6 m 40 25 cm 50 cm3 11 6
1.2
1.2.1
0.2 g
rmin 10 min 70 % 5 mL
-1
HPLC
Waters M820
510 717
1
/min
A/%
B/%
2487 229 nm
100
Novapak C18 20 L
100
21
100
26
100
31
100
A0.05 % B0.05 %
=4 V1 V
-1
1 mLmin 1 31
min
-1
molkg FW
=
www.cnveg.org
201018
2.1
45
1
3
-1
480.9 molkg FW
318.8 molkg
-1
F W 2 1 3 . 9
-1
molkg FW 3 44 %
3
4 266.3 molkg
-1
FW 1 257.6 mol kg
-1
FW
2.2
A 3 B 4 C 1 DE
F
15
Mithen2001 6 5
2 2 5 6
HPLC
2
4--3-
Glucoraphenin
4--3-
4-hydroxyglucobrassicin
4--3-
Glucoraphasatin
-3-
Glucobrassicin
4--3-
4-methoxyglucobrassicin
2.3
5 3 4--3-
70.5 %87.7 %
4 4--3-
www.cnveg.org
46
CHINA VEGETABLES
2010 9
-1
FW
3
5
-1
/molkg FW
4--3-
50.60.4
29.21.5
1
47.73.7
49.70.6
4--3-
4.60.6
3.00.6
3.90.7
2.50.2
4--3-
421.036.4
226.311.3
199.938.5
155.013.1
57.26.9
64.95.2
3.20.9
159.62.4
6.50.9
235.013.9
-3-
1.50.4
5.71.1
2.71.3
4.60.7
3.71.2
8.50.6
4--3-
3.11.9
2.00.6
3.50.9
2.20.8
2.60.5
4.00.6
6 5 4--3-
4--3-4--3-
-3- 4--3- 5
20082008
8 2--3- 64--3-
70 %
Schnitzler W H2002352192-197.
20083581205-1208
Mithen R2001Glucosinolates-biochemistrygenetics and biological activityPlant Growth Regulation3491-103.
Mithen RFaulkner KMagrath RRose PWilliamsom GMarquez J2003Development of isothiocyanate-enriched broccoliand its enhanced
ability to induce phase 2 detoxification enzymes in mammalian cellsTheor Appl Genet106727-734.
Sarikamis GMarquez JMacCormack RRichard NBennettRobert JMithen R2006High glucosinolate broccolia delivery system for
sulforaphaneMol Breeding18219-228.
7 33 44 552
21
47
12
100081
010-82109550
www.cnveg.org