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Article history:
Received 24 March 2009
Received in revised form 4 November 2009
Accepted 6 November 2009
Keywords:
Mentha arvensis
Vigna unguiculata
Intercropping
Green manure
Cymbopopogon martinii
Essential oil yield
a b s t r a c t
A eld experiment was conducted at Central Institute of Medicinal and aromatic Plants (CIMAP), Lucknow,
India in a sandy loam soil (entisol) during 2004 and 2005. Cowpea (Vigna unguiculata L. Walp.) was
intercropped with transplanted menthol mint (Mentha arvensis L.) for green manuring (GM) and for
fodder plus green manuring (F + GM) with four levels of urea N (0, 30, 60, 90 kg N ha1 ). In GM, cowpea
was incorporated in the soil 30 days after sowing (DAS), while in F + GM 50% (alternate) cow pea plants
were used for fodder at 30 DAS and 50% were incorporated in soil at 35 DAS. No signicant differences
were found between GM and F + GM with respect to herb and oil yield of menthol mint and succeeding
palmarosa crop and nitrogen economy. Fresh biomass yield of menthol mint increased by 23.4% and
essential oil yield by 25.2% by cowpea green manure (mean of GM and F + GM) as compared to without GM
across all N levels. The contribution of green manure, as a nitrogen source, was equivalent to 30 kg N ha1
when no fertilizer nitrogen was applied in menthol mint. The residual effect of cowpea GM was studied in
a succeeding crop of fast growing essential oil yielding palmarosa (Cymbopopogon martinii (Roxb.)Wats.
var motia Burk.) over two harvests (July and December). Averaged across N levels green manure resulted
in an increase of 18.5% in the fresh biomass and 17.7% in essential oil yield of palmarosa over no green
manuring.
2009 Elsevier B.V. All rights reserved.
1. Introduction
During the last decade there has been consistent decline in the
prices of menthol mint oil. Therefore, it is critical to minimize the
production inputs to offset the decrease in value. Declining organic
carbon content in Indian soil is of greater cause of concern for future
stability in food production. Yadav (1998) concluded that intensive
and continuous cereal cropping of paddy and wheat with fertilizer
nutrient has caused sharp decline in soil organic carbon status. This
is a major cause of concern in many countries in the world wishing
to increase or sustain the present productivity level.
Menthol mint is an important cash crop in India. It has become
most popular among small holders. In India, mint is cultivated
on approximately 1,60,000 ha of land with annual production of
16,000 t of oil (Singh and Khanuja, 2007). Today, India is the major
global producer and supplier of mint oil and its derivatives in the
world. Mint has a high nutrient demand. Singh (1994) calculated
the N, P and K removal to the extent of 150, 25 and 100 kg ha1 ,
respectively. Application of 160 kg N ha1 for delayed transplanted
menthol mint was recommended by Ram and Kumar (1998). Similar observations were also made by Patra et al. (1998) and Patra et
290
Table 1
Herb and essential oil yield of menthol mint and palmarosa, agronomic efciency (AE) and nitrogen removal as inuenced by cropping system and nitrogen levels (mean of
two years).
Herb yield (t fresh matter ha1 )
Menthol mint
Palmarosa
Menthol mint
Palmarosa
Menthol mint
Palmarosa
Menthol mint
Palmarosa
Total
14.16
17.07
17.87
1.40
12.70
14.79
15.32
0.90
125.6
153.6
160.8
12.6
44.8
51.8
53.7
3.2
0.95
1.23
1.26
0.12
0.17
0.18
0.19
NS
47.7
59.8
61.6
5.5
36.4
43.0
44.6
2.6
84.1
102.8
106.2
7.6
12.03
13.57
15.63
15.85
1.05
94.6
132.0
162.0
200.0
14.45
42.1
47.4
54.8
56.0
3.65
1.15
1.12
1.17
NS
0.18
0.21
0.15
0.02
35.7
50.1
61.5
76.9
6.3
34.2
39.2
45.5
46.1
3.0
69.9
89.3
107.0
123.0
8.8
Treatment
Cropping system
Without GM
Cowpea GM
Cowpea F + GM
LSD (P = 0.05)
GM, green manure; F, fodder; LSD, least signicant difference; P = 0.05, at 5% probability; NS, non signicant.
291
Table 2
Amount of green biomass and N, P and K (kg ha1 ) added by cowpea green manure under different nitrogen levels (mean of two years).
Cropping system
Cowpea GM
N0
N30
N60
N90
Cowpea F + GM
N0
N30
N60
N90
LSD (P = 0.05)
0
0
0
0
1656
2240
3110
3420
135.9
Amount addeda
Green biomass for GMb
4500
4810
6200
6850
20.4
21.7
27.9
30.8.
4.0
4.3
5.6
6.1
12.5
13.5
16.0
18.3
4450
5000
6389
6680
256.7
20.0
22.5
28.8
30.5
1.51
4.0
4.5
5.7
6.0
0.36
12.0
14.0
17.0
18.0
1.21
N, nitrogen.
a
15 cm soil depth.
b
Contained 15% dry matter.
N removal (kg ha1 ) = Dry matter yield (kg ha1 ) N content (%)
in dry matter/100.
3. Results and discussion
3.1. Herb and oil yield of menthol mint and biomass yield of
cowpea
No signicant differences were found between the treatments
GM and F + GM with respect to herb and oil yield of menthol
mint and agronomic efciency. Incorporation of cowpea green
manure signicantly increased the fresh herb yield of menthol
mint by an average (mean of GM and F + GM) of 23.4% and oil
yield by 25.2% over the control without GM (Table 1). Averaged
across N levels, menthol mint yielded 1.23 and 1.26 kg of essential
oil kg1 applied N with cowpea GM and F + GM, respectively and
0.95 kg oil kg1 applied N without GM (Table 1). Menthol mint and
cowpea responded signicantly up to 90 kg N ha1 as the highest N
level 90 kg ha1 was much lower than the 160 kg N ha1 normally
recommended for menthol mint (Ram and Kumar, 1998). The contribution of green manure, as a nitrogen source, was equivalent
to 30 kg N ha1 when no fertilizer nitrogen was applied (Fig. 1).
Pandey and Pendleton (1986) also reported 28 kg N ha1 contribution of soyabean green manure incorporated at 42 days after
sowing in maize when no nitrogen was applied. In a similar study
Fig. 1. Effect of nitrogen levels with and without cowpea green manure on menthol
mint yield.
using soil and fertilizer labeling technique with 15 N on estimation of dinitrogen xation by cowpea and concurrent transfer of
x N to maize, Patra et al. (1986) reported a nitrogen benet of
about 30 kg ha1 to the companion maize in maize cowpea intercropping. A signicant increase in biomass yield of legume green
manure crops with the application of N up to 60 kg ha1 was also
recorded by Jeranyama et al. (2000) and Yadav (2004). The higher
biomass of mint and intercrop cowpea is understandable in view
of the fact that in the early stages, growth of menthol mint being
extremely low, cowpea could be able to utilize full advantages of
available growth resources whereas in the subsequent stage mint
did not face any competition with the companion crop which was
incorporated in soil at 3035 days after sowing. Trenbath (1979)
postulated that under non-limiting soil conditions, crops of different heights, like wheat and alfalfa, have an advantage in terms of
daily net photosynthesis.
Although the contribution of cowpea green manure was small
it may be signicant for small farmers whose cash input is limited. When cowpea was incorporated as green manure at 30 and
35 days after sowing, it produced 4450 (N0 GM) to 6850 (N90 ,
GM) kg green biomass which contained 2030.8 kg N, 4.06.1 kg P
and 12.018.3 kg K ha1 (Table 2). In F + GM treatment 50% (alternate) plants were cut at 30 DAS for fodder; the remaining 50%
plants were allowed to grow for ve more days till 35 DAS accumulated almost same amount of biomass as 100% plants at 30
DAS as it was the grand growth period of cowpea. There was no
interplant competition, soil moisture was sufcient and weather
conditions were suitable. Maximum temperature 3540 C, minimum temperature 2025 C and bright sunny days induced very
fast growth of cowpea as all the cultivars of cowpea are considered as warm season, adapted to hot and drought conditions and
respond well to optimum soil moisture. Aikins and Afuakwa (2008)
also reported about 50% increase in plant height, 25% increase
in stem girth and 100% increase in number of leaves per plant
in cowpea within a period of one week (between four and ve
weeks after emergence) at Kumasi, Ghana. Besides shoot biomass,
un-quantied amounts of decomposing roots and nodules might
have contributed nitrogen to the menthol mint (Patra et al., 1989).
Cowpea F + GM also yielded 16563420 kg ha1 green fodder as a
bonus which is of signicant value during summer in mint growing areas. The benecial effect of green manuring has also been
reported in other crops (not mint); ricewheat system by Yadav
et al. (2000) and Yadav (2004), in maize by Pandey and Pendleton
(1986) and Jeranyama et al. (2000) and in tomatomaize system
by Thonnissen et al. (2000). In intercropping system involving
wheat and alfalfa, protein yield of wheat crop was signicantly
292
Table 3
Soil organic carbon content and available nitrogen after harvest of menthol mint as
inuenced by cowpea green manure under different N levels (mean of two years)a .
Organic carbon
content (%)
Cropping system
Without GM
Cowpea GM
Cowpea F + GM
LSD (P = 0.05)
0.326
0.336
0.336
NS
161.2
173.8
175.6
9.3
0.328
0.331
0.334
0.335
NS
162.9
169.7
173.6
174.6
10.8
Treatment
In top 15 cm soil; N, nitrogen; GM, green manure; F, fodder; NS, non signicant.
Acknowledgement
The authors are thankful to the Director, Central Institute of
Medicinal and Aromatic plants, Lucknow for providing facilities.
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