Primary Barrier

Biological

g
Safety
y Level,,
BSL

CDC

BMBL
BMBL
BSL
BSL

BMBL
BMBL
BSL
BSL

BMBL
BMBL
BSL
BSL

BMBL
BMBL
BSL
BSL

BSC Functions
Functions
Pressure Difference Control
Dilute Contaminated Air
Air Distribution
Cleanness Control
Exhaust Filtration

BSC Classification

Type A1
Type
yp A2

Type B1

Type B2

Class I BSC

HEPA

0 38 m/sec 75 ft/min
0.38

HEPA

HEPA
HEPA

DOP, 0.3m, 99.97%
HEPA0.3
m


HEPA

Class I BSC

HEPA filtration
High
g efficiency
y pparticulate air(HEPA)
(
) filter

Efficiently removes microscopic

contaminants from air
Removes particles 0.3 m with a minimum efficiency of 99.97%
DOES NOT capture volatile chemicals or gases

HEPA
A
B

A
A1
0.38 m/sec(75fpm)
A20.5m/sec
A1
A2

30%HEPA70%
HEPA
30%70%
HEPA
t b l t flow
turbulent
fl

A1

A1

A.
A
B.
C. HEPA
D HEPA
D.
Supply HEPA filter
E. Rear plenum
F.

A1

A1

A2

A2

A. front opening
B. sash
C. exhaust HEPA filter
D. supply HEPA filter
E positive pressure plenum
E.
F. negative pressure plenum

BSC Class II,

II type A2

BSC Fl
Flow Si
Simulation
l ti
(Class 2 type A2)

B1

B1

BHEPA

0.5 m/sec100 fpm

B1B2

B1

B1

B1
B1

30%
HEPA 70%
HEPA70%
HEPA


B

B1

B1

A. front opening
p
g
B. sash
C. exhaust HEPA filter
D. supply HEPA filter
E. negative
g
pressure
p
exhaust p
plenum
F. blower
G. additional HEPA filter for air
supply
Note: The cabinet exhaust needs to be
connected to the building exhaust.

B1
B1

Bench Top
Top

B2

B2

100%
100%

HEPA

HEPA
HEPA

0.5 m/sec 100 fpm

B2

B2

HEPA
HEPA

0 57 m3/s
0.57

safety interlock
interlock

B2

B2

A. front opening
B. sash
C. exhaust HEPA filter
D supply HEPA filter
D.
E. negative pressure exhaust plenum
F. supply blower G. filter screen
Note: The cabinet exhaust needs to
b connected
be
t d to
t the
th building
b ildi exhaust
h t
system.

10-5ml/sec

HEPA



HEPA
HEPA
HEPA

0.124 kPa
0.5 inWG

BSC Class III

A.
B.
C.
D.
E.

glove ports
sash
exhaust HEPA filter
supply HEPA filter
pass-through
p
g box

(Laminar)

(Laminar)
Laminar Flow Clean Bench

Supply air passes through HEPA filter over work surface, then is exhausted to
the room
Exhaust air actually blows into operators
operator s face
Never handle toxic or infectious materials in Laminar Flow Clean Bench


Class 2 type B2 100%

Class 3 BSC

Biological
safety Level,
BSL

1~3
1~3
4

I
II
A1,A2 B1,B2

III

m/sec

0.38

II, A1
II, A2
B3

HEPA FILTER
HEPA FILTER

0.38

70%HEPA
30%HEPA

0.50

A1

II, B1

0.50

30%HEPA
70%HEPA

HEPA FILTER

II, B2

0.50

HEPA FILTER

III

N/A

Secondary Containment
Secondar
HVAC&R System
y
EF
2
HUM

HEPA

SA 3000CMH
EA 3150CMH

OA

HEPA
UV BI/BO

HEPA

SA 150CMH SA 100CMH
EA 200CMH EA 150CMH

VIRUS P3

BUFFER-1 A/L-1

BSC

ESF
2

ACS
2

MF
PF

SA

MAU
1

EA

WASTE

CARRIDOR

C.D.

C.D.
RF


Hard Connect(B type)
(A type)

NSF 49

--

Bag in/Bag out
HEPA

--

Class 2

//

//

Interlock

 TAFLogo
(ISO17025)

Logo

Certification Report for Biological Safety

XX cm
XX cm

HEP
HEP

1.

--

--

2.HEPA

3.

4
4.

5.

--

--

1.
(1) 0~500
0 500 Pa5.0Pa
Pa5 0Pa
(2) 500Pa

(3)
a.
b.30 10%
(4) ___________________________

2. HEPA
((1)) Smoke Generator10ug/L1.3~11cm2

g
510-4m3/s10% & Aerosol PhotometerDownstream
Upstream 10-3%
(2) DOP Photometer scanner
HEPA 50mm/sec
(3) 0.01%
(4) ________________________________________
(5)

__________________________

4.
(1) 10%
((2))

15cm

30 cm 200~400
Lux
((3)) 850~1600 Lux 800~1880
Lux
160 Lux
500 Lux

(4) :Lux

cm

15

45

75

105

135

165

198

(5) __________________________

5.
(1) 2.5m rms
((2))
(3) 10Hz 10kHz
510-6 m rms
510-5 m rms
(4) :10-4m rms

Net

Amplitude
p

(5) __________________________

7.
((1))
(2)
(3)
i. UL3101-112.4
ii. 110 kg

1.6 mm

NSF-49ENASJIS
ISO

8.
(1) 0.02m/s
(2)
10cm
10cm
30cm
30cm

1515 cm15cm

(3) 0.03m/s0.3~

0.5m/s20%

25%

(4)
m/s

(5) ___________________________

10.
(1)
(2)

i.
10cm
ii 2.5cm15cm
ii.
2 5
15

iii. 4.0cm

iv. 5cm

11.
(1)
(2) 3.5
(3)
(4) ___________________________

12.
(1) 2%
(2) Down-flow HEPA
HEPA 50%
HEPA
50%
m3/s
(3) 10
(4) :mmAQ

CDC/NIH, 2000Primary Containment For

Biohazards: Selection, Installation And Use

Of Biological Safety Cabinets

NSF (National Sanitation Foundation)

Foundation),2008
2008
NSF 49 Class II (Laminar Flow) Biosafety
%
Cabinetry
Europe StandardEN12469, Biological
(5)

___________________________
Safety Cabinets
AS /NZS 2252Biological safety Cabinets
13. SF6

(1)SF6 110-7cc/s
(2)SF6 500Pa30 2.5
m/s Probe


19551

035912296

ISOTAF