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Relatively easy to get “bulk” rates of transformation of specific chemicals, but the
major question is, “who is doing it?”
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(1-3) 1. Radiotracers: Use of radioactive isotopes. For example C, S, P.
“bulk”
methods Biodegradation of specific compounds:
a. Spike labeled compounds into environmental samples. For example:
Pesticide: CO2 evolution Æ mineralization
CO2
Substrate
example: carbon
⎡
⎢
δ 13C = ⎣
( 13
C 12
C )
sample
-( 13
C 12
C ) std.
⎤
⎥
⎦ . 1000
( C)
13
C 12
std.
15 13
Heterotrophs are enriched in N (~3 pp thousand) and depleted in C (~ -2 pp
thousand)
Predators feed on
*
3 previous organisms
Fractionation excrete
enriched
* 1
*
lighter isotope 14N &
keep 15N so that they in � 15N Gives idea
� 15N
depleted in � 13C
Primary producer
� 13C
• Kinetics
• Tolerance
dB
= µB biomass
dt
Monod Equation
dt K s + ⎣⎡C⎦⎤
Limiting cases:
dB
dB µ ⎡C⎤ B
more realistic b) [C] << Ks = max ⎣ ⎦
environmental 1st order dt Ks
situation
Kinetics!
dC 1 ⎛ µmax ⎡⎣C⎤⎦ B ⎞
= ⎜ ⎟
dT Y ⎜⎝ K s + ⎣⎡C⎦⎤ ⎟⎠
Get these
“weeds” in lab
Tolerance Limits
Example:
is usually calostrophic
breakdown
µ
Temperature
1.89, Environmental Microbiology Lecture 17
Prof. Martin Polz Page 3 of 4
• Temperature: Q10 Rule within tolerance limit, there is a ~2–fold increase in
activity for each 10oC increase in temperature.