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ENZYME TECHNOLOGY
Enzyme:-A protein with catalytic properties due to its power of
specific activation
Introduction
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cont.
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Firefly bioluminescence
is produced by an
oxidation
reaction
catalyzed
by
the
enzyme firefly luciferase
The oxidized substrate
(product of the reaction)
is in an electronically
excited state that emits
light as it returns to the
ground state.
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Enzyme..
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Specificity of Enzymes
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Nature of Enzyme
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Enzyme structure
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cont
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cont..
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Enzyme Nomenclature
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Enzymes are commonly named by adding a suffix "ase" to the root name of the substrate molecule it is
acting upon. For example, Lipase catalyzes the
hydrolysis of a lipid triglyceride. Sucrase catalyzes the
hydrolysis of sucrose into glucose and fructose.
A few enzymes discovered before this naming system
was devised are known by common names. Examples
are pepsin, trypsin, and chymotrypsin which catalyzes
the hydrolysis of proteins.
The latest systematic nomenclature system known as the
International Enzyme Commission (IEC) system is based
upon the type of reaction catalyzed.
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Enzyme catalysis
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E
E
E
Enzymesubstrate
complex
Enzyme may be
used again
P
P
Reaction coordinate
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ENZYME KINETICS
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Michaelies-Menten/rapid equilibrium
Approach(1913)
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Michaelis constant
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Eadie-Hofstee
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